Fluorescence Dye Reference Chart

Free reference for microscopy labs. Updated 2026.

DyeTargetExcitationEmissionColor
Hoechst 33342Nucleus405nm460nmBlue
Alexa Fluor 488 ConAER/Cytoplasm470nm525nmGreen
SYTO 14Nucleoli480nm500nmGreen-Yellow
MitoTracker Deep RedMitochondria640nm662nmFar Red
Phalloidin (various)ActinVariesVariesVaries
WGA (various)Golgi/PlasmaVariesVariesVaries

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