CellInsight CX7 in Cancer Research
Curated literature review of peer-reviewed cancer studies that used the CellInsight CX7 High-Content Screening Platform. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
The CellInsight CX7 platform is widely adopted in oncology and drug-discovery programmes for multi-well, multi-parameter phenotypic screening. The papers below illustrate high-content imaging of cancer cell lines, spheroids, and engineered models for target validation, compound profiling, and mechanism-of-action studies.
A reversible broad-spectrum antiviral targets the human V-ATPase VO domain.
Cell type: HEK293 cells, A549 lung cancer cells, primary cells, stem cells, epithelial cells
Imaging technique: live-cell imaging, confocal microscopy, brightfield microscopy
Disease area: cancer, infectious disease, virology, COVID-19, drug screening
Increasing threats of viral disease underscore the urgent need for broad-spectrum antiviral drugs (BSADs). Host proteins utilized by human pathogenic viruses are key BSAD targets. The vacuolar-type H+-ATPase (V-ATPase) has been identified as a proviral factor for most pH-dependent enveloped viruses classified as pandemic threats. We report here the discovery of cladoniamide A (CA), a V-ATPase inhibitor with single-digit nanomolar antiviral activity and a high selectivity index (SI: 103-104) agai...
Research square — 2026 — PMID 42282011
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Single-cell transcriptomics uncovers heterogenous cell clusters and the biomarker FUT11 in ovarian cancer progression.
Cell type: fibroblasts, macrophages, endothelial cells, epithelial cells
Imaging technique: high-content imaging
Disease area: cancer, virology, fibrosis
BACKGROUND: Ovarian cancer (OC) is the most lethal of gynecological cancers and presents a poor prognosis due to difficulty in early diagnosis, extensive abdominal metastasis and chemo-resistance. We utilized single-cell transcriptomics to deconvolute intratumoral heterogeneity and identify biomarkers and therapeutic targets. METHODS: Single-cell RNA sequencing (scRNA-seq) were performed with 15 patient samples. Metastatic and chemo-resistant epithelial subclusters were discovered by copy-number...
Cancer cell international — 2026 — PMID 41987229
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A role for CASM in the repair of damaged Golgi architecture.
Cell type: HeLa cells, HEK293 cells, macrophages, neurons
Imaging technique: fluorescence microscopy, high-content imaging, confocal microscopy
Disease area: cancer, Alzheimer's disease, infectious disease, virology, bacterial infection, inflammation
The term CASM describes a process in which MAP1LC3B/LC3B and other Atg8-family proteins are covalently ligated to lipids in damaged endomembranes. While CASM is commonly described as a cytoprotective response to multiple types of membrane damage, how CASM helps cells maintain homeostasis is still unclear. Here, we show that CASM maintains Golgi apparatus architecture following the loss of TRIM46, a ubiquitin ligase with roles in microtubule organization. TRIM46 deficient cells were notable for e...
Autophagy — 2026 — PMID 42115886
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Identification of host lncRNAs that impact Venezuelan equine encephalitis virus TC-83 replication.
Cell type: A549 lung cancer cells, primary cells, neurons
Imaging technique: high-content imaging
Disease area: cancer, infectious disease, virology, COVID-19, inflammation
Venezuelan equine encephalitis virus (VEEV) causes encephalitis in humans and equids, and there are no vaccines or therapeutics for humans. In recent years, non-coding RNAs have emerged as critical regulatory factors affecting different cellular pathways. Specifically, long non-coding RNAs (lncRNAs) have been identified as regulators of antiviral pathways; however, their role in VEEV infection has not been assessed. Here, we show differential expression of several lncRNAs in primary mouse target...
Journal of virology — 2026 — PMID 42023950
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Computational epitope heterogeneity analysis in immunostainings from antibody-dilution series.
Cell type: HeLa cells, neurons
Imaging technique: high-content imaging
Disease area: cancer, diabetes
Antibodies are widely used in life sciences and medical therapy. Broadly applicable methods to determine epitope heterogeneity in immunostaining systems are missing. Here, we present a simple-to-use approach to characterize and quantify antibody binding properties that constitute the staining directly in the system of choice. We determine an epitope heterogeneity on the basis of a computational analysis of antibody-dilution immunofluorescence stainings. This allows us to choose signal-specificit...
Communications biology — 2026 — PMID 41629448
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RNA editing for the treatment of alpha-1 antitrypsin deficiency.
Cell type: stem cells, iPSCs, hepatocytes
Imaging technique: high-content imaging, brightfield microscopy
Disease area: cancer, infectious disease, virology, fibrosis, inflammation
Alpha-1 antitrypsin deficiency (AATD) is both a gain- and loss-of-function disease that impacts the liver and lung. Most severe cases result from a homozygous missense mutation in the SERPINA1 gene (Z mutation). While current therapies and those in development may ameliorate lung or liver disease, few are designed to address both. We have developed SERPINA1-994, an N-Acetylgalactosamine-conjugated chemically modified oligonucleotide that elicits adenine-to-inosine RNA editing using endogenous ad...
Nucleic acids research — 2026 — PMID 41533577
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Interleukin-7R and chemokine receptor type 7 differentially regulate metabolism in CD4+ and CD8+ T cells.
Cell type: fibroblasts
Imaging technique: confocal microscopy
Disease area: cancer, inflammation
Naïve T cells are highly metabolically active, maintaining homeostatic function as well as continuously moving and surveying lymph nodes for dendritic cells (DCs) presenting cognate antigen. T-cell metabolism is thought to change throughout development: Naïve T cells have been found to predominantly utilize catabolism for naïve T-cell homeostasis while T-cell activation leads effector T cells to become glycolytic. There is still relatively less known about how individual and combinations of mole...
ImmunoHorizons — 2026 — PMID 41491655
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From in silico prediction to experimental validation: Identification of drugs and novel synergistic combinations that inhibit growth of inflammatory breast cancer cells.
Cell type: MCF-7 breast cancer cells, stem cells, macrophages, epithelial cells
Imaging technique: high-content imaging, brightfield microscopy
Disease area: cancer, virology, drug screening
Drug repurposing offers a promising approach for identifying novel treatments, especially for rare cancers like inflammatory breast cancer (IBC), an aggressive type with limited therapeutic options. Here, we present a comprehensive validation and verification study of compounds identified through two computational approaches: Literature Wide Association Studies (LWAS) and Gene Reversal Rate (GRR), using orthogonal cell viability assays in 2D models across IBC and non-IBC cell lines. In the SUM14...
bioRxiv : the preprint server for biology — 2025 — PMID 41427337
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ATG16L1 controls mammalian vacuolar proton ATPase.
Cell type: HeLa cells, HEK293 cells, macrophages, neurons, epithelial cells
Imaging technique: high-content imaging, confocal microscopy
Disease area: cancer, Alzheimer's disease, infectious disease, virology, bacterial infection, fibrosis, inflammation
The mechanisms governing mammalian proton pump V-ATPase function are of fundamental and medical interest. The assembly and disassembly of cytoplasmic V1 domain with the membrane-embedded V0 domain of V-ATPase is a key aspect of V-ATPase localization and function. Here, we show that the mammalian protein ATG16L1, primarily appreciated for its role in canonical autophagy and in noncanonical membrane atg8ylation processes, controls V-ATPase. ATG16L1 knockout elevated V-ATPase activity, increased V1...
The Journal of cell biology — 2025 — PMID 40923996
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Drug-induced phospholipidosis as an artifact in antiviral drug repurposing.
Cell type: A549 lung cancer cells, HepG2 hepatoma cells, neurons, hepatocytes, epithelial cells
Imaging technique: live-cell imaging, high-content imaging, confocal microscopy
Disease area: cancer, infectious disease, virology, COVID-19
Drug repurposing in principle can speed antiviral drug discovery. Among the molecules most frequently advanced in such repurposing efforts are a group of structurally diverse cationic amphiphilic drugs (CADs). While CADs have shown micromolar to mid-nanomolar antiviral activity in cell-based assays, they can induce phospholipidosis, confounding in COVID-19 repurposing. A barrier to the identification of phospholipidosis inducers has been the involved nature of the microscopy assays used to chara...
bioRxiv : the preprint server for biology — 2025 — PMID 41332603
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Early intervention with extracellular vesicles derived from human umbilical cord mesenchymal stem cells enhances survival and functional recovery after spinal cord injury in rats.
Cell type: stem cells, macrophages, neurons, endothelial cells
Imaging technique: confocal microscopy
Disease area: cancer, infectious disease, COVID-19, inflammation
INTRODUCTION: Spinal cord injury (SCI) is a devastating condition with high mortality and limited treatment options. Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have emerged as promising cell-free therapies due to their immunomodulatory and neuroprotective properties. Here, we evaluated the therapeutic potential of EVs derived from human umbilical cord MSCs in a rat model of SCI. METHODS: Adult male Wistar rats were randomized into three groups: control, SCI, and SCI treated...
Frontiers in pharmacology — 2025 — PMID 41311838
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Characterizing and controlling CRISPR repair outcomes in nondividing human cells.
Cell type: primary cells, stem cells, iPSCs, neurons
Imaging technique: confocal microscopy
Disease area: cancer, Alzheimer's disease, virology
Genome editing is poised to revolutionize treatment of genetic diseases, but poor understanding and control of DNA repair outcomes hinders its therapeutic potential. DNA repair is especially understudied in nondividing cells like neurons, limiting the efficiency and precision of genome editing in many clinically relevant tissues. Here, we address this barrier by using induced pluripotent stem cells (iPSCs) and iPSC-derived neurons to examine how postmitotic human neurons repair Cas9-induced DNA ...
Nature communications — 2025 — PMID 41249169
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The Neurolipid Atlas: a lipidomics resource for neurodegenerative diseases.
Cell type: stem cells, iPSCs, fibroblasts, macrophages, neurons
Imaging technique: fluorescence microscopy, confocal microscopy
Disease area: cancer, Alzheimer's disease, Parkinson's disease, virology, inflammation
Lipid alterations in the brain have been implicated in many neurodegenerative diseases. To facilitate comparative lipidomic research across brain diseases, we establish a data common named the Neurolipid Atlas that we prepopulated with isogenic induced pluripotent stem cell (iPS cell)-derived lipidomics data for different brain diseases. Additionally, the resource contains lipidomics data of human and mouse brain tissue. Leveraging multiple datasets, we demonstrate that iPS cell-derived neurons,...
Nature metabolism — 2025 — PMID 40983680
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Host cell and viral protease targets of human SERPINs identified by in silico docking.
Cell type: HEK293 cells, A549 lung cancer cells, macrophages, epithelial cells
Imaging technique: high-content imaging
Disease area: cancer, infectious disease, virology, COVID-19, cardiovascular disease, fibrosis, inflammation
Serine protease inhibitors (SERPINs) are involved in various physiological processes and diseases, such as inflammation, cancer metastasis, and neurodegeneration. Their role in viral infections is poorly understood, as their expression patterns during infection and the range of proteases they target have yet to be fully characterized. Here, we show widespread expression of human SERPINs in response to respiratory virus infections, both in bronchioalveolar lavages from COVID-19 patients and in po...
The EMBO journal — 2025 — PMID 40926121
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Phosphorylation of plectin by Akt3 promotes triple-negative breast cancer cell invasive migration.
Cell type: HEK293 cells, stem cells
Imaging technique: high-content imaging, confocal microscopy
Disease area: cancer, infectious disease, virology, diabetes
Triple-negative breast cancer (TNBC) lacks targeted therapeutics and is aggressive with a poor prognosis. The phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway, frequently deregulated in cancers, plays crucial roles in tumorigenesis and cancer progression. However, the distinct functions of the three Akt isoforms (Akt1, Akt2, Akt3) in these processes are not well understood. Here, we focus on Akt3, the least-studied Akt isoform, which is overexpressed in 28% of TNBC cases and significan...
iScience — 2025 — PMID 41079612
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Novel regulators of heparan sulfate proteoglycans modulate cellular uptake of α-synuclein fibrils.
Cell type: HeLa cells, HEK293 cells, SH-SY5Y neuroblastoma cells, stem cells, iPSCs, macrophages, neurons, epithelial cells
Imaging technique: fluorescence microscopy, live-cell imaging, high-content imaging, confocal microscopy, Z-stack imaging
Disease area: cancer, Parkinson's disease, infectious disease, virology
Synucleinopathies are characterized by the accumulation and propagation of α-synuclein (α-syn) aggregates throughout the brain, leading to neuronal dysfunction and death. In this study, we used an unbiased FACS-based genome-wide CRISPR/Cas9 knockout screening to identify genes that regulate the entry and accumulation of α-syn preformed fibrils (PFFs) in cells. We identified key genes and pathways specifically implicated in α-syn PFFs intracellular accumulation, including heparan sulfate proteogl...
Communications biology — 2025 — PMID 41053297
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Exploiting dysregulated iron homeostasis to eradicate persistent high-grade serous ovarian cancer.
Cell type: HeLa cells, stem cells, fibroblasts, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, virology, fibrosis
Treatments for high-grade serous ovarian cancer (HGSOC) are initially effective but most invariably fail. Although they can successfully suppress the bulk of the tumour cell population, residual cancer cells can enter alternative therapy-resistant cell fates highlighted by proliferative arrest. Understanding the nature of these fates and how cells may resume uncontrolled proliferation will lead to the development of new treatments for HGSOC. In this study, we examine the response of HGSOC cells ...
Cell death discovery — 2025 — PMID 40998801
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A role for CASM in the repair of damaged Golgi architecture.
Cell type: HeLa cells, HEK293 cells, macrophages, neurons
Imaging technique: high-content imaging, confocal microscopy
Disease area: cancer, Alzheimer's disease, infectious disease, virology, bacterial infection, inflammation
The term CASM describes a process in which LC3 and other Atg8 proteins are covalently ligated to lipids in damaged endomembranes. While CASM is commonly described as a cytoprotective response to multiple types of membrane damage, the ways in which CASM helps cells maintain homeostasis are still unclear. Here, we show that CASM contributes to the maintenance or repair of Golgi apparatus architecture following the loss of TRIM46, a ubiquitin ligase with roles in microtubule organization. TRIM46-de...
bioRxiv : the preprint server for biology — 2025 — PMID 40950167
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Identification of SRSF9 through pooled shRNA screening links BNIP3 splicing to autophagy and metabolic reprogramming in breast cancer.
Cell type: HEK293 cells, MCF-7 breast cancer cells, primary cells
Imaging technique: fluorescence microscopy, high-content imaging, confocal microscopy
Disease area: cancer, virology
Autophagy is a critical catabolic process that maintains cellular homeostasis, yet the role of alternative splicing in regulating hypoxia-induced autophagy remains largely unexplored. Here, through a pooled shRNA screen of RNA-binding proteins (RBPs) in hypoxic breast cancer cells, we identified the splicing factor SRSF9 as a key regulator. We found that SRSF9 expression is significantly reduced under hypoxia, while its restoration diminishes autophagosome formation. SRSF9 directly controls the ...
The Journal of biological chemistry — 2025 — PMID 40675219
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Therapeutic potential of human mesenchymal stromal cell-derived mitochondria in a rat model of surgical digestive fistula.
Cell type: stem cells, fibroblasts, macrophages, neurons, epithelial cells
Imaging technique: fluorescence microscopy, live-cell imaging, high-content imaging, confocal microscopy
Disease area: cancer, infectious disease, fibrosis, inflammation
Mitochondria are central to cellular energy metabolism and play a critical role in tissue regeneration. Mitochondrial dysfunction contributes to a range of degenerative conditions and impaired wound healing, driving increasing interest in mitochondrial transplantation as a novel therapeutic strategy. Gastrointestinal wound healing is particularly susceptible to failure, with complications such as post-surgical fistula formation commonly occurring after procedures like sleeve gastrectomy. Mitocho...
Scientific reports — 2025 — PMID 40783579
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Oral dosing of the nucleoside analog obeldesivir is efficacious against RSV infection in African green monkeys.
Cell type: HeLa cells, stem cells
Imaging technique: fluorescence microscopy
Disease area: cancer, infectious disease, virology, bacterial infection, COVID-19
Respiratory syncytial virus (RSV) is a significant cause of morbidity and mortality in high-risk populations. Although prophylactic options are available, there are no effective oral therapeutics for RSV infection. Obeldesivir (ODV) is an orally bioavailable prodrug of the nucleoside analog GS-441524, which is converted intracellularly to its active nucleoside triphosphate and inhibits the RSV RNA polymerase. Here we report the potent antiviral activity of ODV against geographically and temporal...
Nature communications — 2025 — PMID 40645925
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An injectable 2.5% cross-linked polyacrylamide hydrogel (2.5 iPAAG) demonstrates no neurotoxicity in human induced pluripotent stem cells-derived iCell® GlutaNeurons.
Cell type: SH-SY5Y neuroblastoma cells, stem cells, iPSCs, neurons
Imaging technique: fluorescence microscopy
Disease area: cancer, Alzheimer's disease, inflammation, drug screening
INTRODUCTION: Arthrosamid®, Arthramid®, Bulkamid®, and Mictamid® are products for the management of osteoarthritis and female urinary incontinence. All four products include the same injectable hydrogel consisting of 2.5% crosslinked polyacrylamide termed 2.5 iPAAG that is polymerized from the neurotoxic compound acrylamide. METHODS: To investigate whether 2.5 iPAAG demonstrates any neurotoxic effects in vitro, human iCell® Glutaneurons were exposed to concentrations of up to 20% (w/w) 2.5 iPAAG...
Frontiers in toxicology — 2025 — PMID 40626196
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ATG9A facilitates the closure of mammalian autophagosomes.
Cell type: HeLa cells, HEK293 cells, macrophages, neurons, epithelial cells
Imaging technique: fluorescence microscopy, high-content imaging, confocal microscopy
Disease area: cancer, infectious disease, virology, bacterial infection, inflammation
Canonical autophagy captures within specialized double-membrane organelles, termed autophagosomes, an array of cytoplasmic components destined for lysosomal degradation. An autophagosome is completed when the growing phagophore undergoes ESCRT-dependent membrane closure, a prerequisite for its subsequent fusion with endolysosomal organelles and degradation of the sequestered cargo. ATG9A, a key integral membrane protein of the autophagy pathway, is best known for its role in the formation and ex...
The Journal of cell biology — 2025 — PMID 39745851
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Near Infrared Biomimetic Hybrid Magnetic Nanocarrier for MRI-Guided Thermal Therapy.
Cell type: stem cells, macrophages
Imaging technique: high-content imaging
Disease area: cancer, COVID-19
Cell-membrane hybrid nanoparticles (NPs) are designed to improve drug delivery, thermal therapy, and immunotherapy for several diseases. Here, we report the development of distinct biomimetic magnetic nanocarriers containing magnetic nanoparticles encapsulated in vesicles and IR780 near-infrared dyes incorporated in the membranes. Distinct cell membranes are investigated, red blood cell (RBC), melanoma (B16F10), and glioblastoma (GL261). Hybrid nanocarriers containing synthetic lipids and a cell...
ACS applied materials & interfaces — 2025 — PMID 38973727
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A multiparametric anti-aging CRISPR screen uncovers a role for BAF in protein synthesis regulation.
Cell type: primary cells, fibroblasts
Imaging technique: live-cell imaging, high-content imaging, confocal microscopy
Disease area: cancer, virology, bacterial infection, cardiovascular disease
Progeria syndromes are very rare, incurable premature aging conditions recapitulating most aging features. Here, we report a whole genome, multiparametric CRISPR screen, identifying 43 genes that can rescue multiple cellular phenotypes associated with progeria. We implement the screen in fibroblasts from Néstor-Guillermo Progeria Syndrome male patients, carrying a homozygous A12T mutation in BAF. The hits are enriched for genes involved in protein synthesis, protein and RNA transport and osteocl...
Nature communications — 2025 — PMID 39956852
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Serine starvation suppresses the progression of esophageal cancer by regulating the synthesis of purine nucleotides and NADPH.
Cell type: macrophages, neurons
Imaging technique: high-content imaging
Disease area: cancer, infectious disease, virology
Serine metabolism provides important metabolic intermediates that support the rapid proliferation of tumor cells. However, the role of serine metabolism in esophageal squamous cell carcinoma (ESCC) and the underlying mechanism remains unclear. Here, we show that serine starvation predominantly inhibits ESCC cell proliferation by suppressing purine nucleotides and NADPH synthesis. Mechanistically, serine depletion led to the accumulation of aminoimidazole carboxamide ribonucleoside (AICAR), an in...
Cancer & metabolism — 2025 — PMID 39948566
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AMG 193, a Clinical Stage MTA-Cooperative PRMT5 Inhibitor, Drives Antitumor Activity Preclinically and in Patients with MTAP-Deleted Cancers.
Cell type: macrophages
Imaging technique: high-content imaging
Disease area: cancer, virology
One of the most robust synthetic lethal interactions observed in multiple functional genomic screens has been the dependency on protein arginine methyltransferase 5 (PRMT5) in cancer cells with MTAP deletion. We report the discovery of the clinical stage MTA-cooperative PRMT5 inhibitor AMG 193, which preferentially binds PRMT5 in the presence of MTA and has potent biochemical and cellular activity in MTAP-deleted cells across multiple cancer lineages. In vitro, PRMT5 inhibition induces DNA damag...
Cancer discovery — 2025 — PMID 39282709
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A new class of natural anthelmintics targeting lipid metabolism.
Cell type: cell lines
Imaging technique: time-lapse microscopy, high-content imaging, confocal microscopy, brightfield microscopy
Disease area: cancer, infectious disease, bacterial infection, diabetes
Parasitic helminths are a major global health threat, infecting nearly one-fifth of the human population and causing significant losses in livestock and crops. Resistance to the few anthelmintic drugs is increasing. Here, we report a set of avocado fatty alcohols/acetates (AFAs) that exhibit nematocidal activity against four veterinary parasitic nematode species: Brugia pahangi, Teladorsagia circumcincta and Heligmosomoides polygyrus, as well as a multidrug resistant strain (UGA) of Haemonchus c...
Nature communications — 2025 — PMID 39746976
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From N-0385 to N-0920: Unveiling a Host-Directed Protease Inhibitor with Picomolar Antiviral Efficacy against Prevalent SARS-CoV-2 Variants.
Cell type: HEK293 cells, hepatocytes, epithelial cells
Imaging technique: high-content imaging
Disease area: cancer, infectious disease, virology, COVID-19, inflammation
The worldwide spread of new SARS-CoV-2 variants emphasizes the need to diversify existing therapeutic strategies. TMPRSS2, a host protease crucial for SARS-CoV-2 entry, has garnered significant research attention as a potential target for therapeutic intervention. Here, we optimized N-0385, a previously reported TMPRSS2 ketobenzothiazole-based peptidomimetic inhibitor, by screening 135 derivatives for target affinity and antiviral potency. Among the top candidates, N-0695 exhibited low nanomolar...
Journal of medicinal chemistry — 2025 — PMID 40163818
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mTORC1 activity oscillates throughout the cell cycle, promoting mitotic entry and differentially influencing autophagy induction.
Cell type: HeLa cells, U2OS osteosarcoma cells, fibroblasts
Imaging technique: high-content imaging, confocal microscopy
Disease area: cancer
Mechanistic Target of Rapamycin Complex 1 (mTORC1) is a master metabolic regulator that is active in nearly all proliferating eukaryotic cells; however, it is unclear whether mTORC1 activity changes throughout the cell cycle. We find that mTORC1 activity oscillates from lowest in mitosis/G1 to highest in S/G2. The interphase oscillation is mediated through the TSC complex but is independent of major known regulatory inputs, including Akt and Mek/Erk signaling. By contrast, suppression of mTORC1 ...
Cell reports — 2024 — PMID 39067023
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Effects of zinc in podocytes and cortical collecting duct in vitro and Dahl salt-sensitive rats in vivo.
Cell type: HeLa cells, neurons, epithelial cells
Imaging technique: confocal microscopy
Disease area: cancer, diabetes, fibrosis, inflammation
Zinc is one of the essential divalent cations in the human body and a fundamental microelement involved in the regulation of many cellular and subcellular functions. Experimental studies reported that zinc deficiency is associated with renal damage and could increase blood pressure. It was proposed that zinc dietary supplementation plays a renoprotective role. Our study aimed to investigate the effects of zinc on intracellular signaling in renal cells and explore the correlation between dietary ...
The Journal of biological chemistry — 2024 — PMID 39276935
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Cytokine-armed pyroptosis induces antitumor immunity against diverse types of tumors.
Cell type: HEK293 cells, primary cells, macrophages
Imaging technique: confocal microscopy
Disease area: cancer, virology, bacterial infection, inflammation
Inflammasomes are defense complexes that utilize cytokines and immunogenic cell death (ICD) to stimulate the immune system against pathogens. Inspired by their dual action, we present cytokine-armed pyroptosis as a strategy for boosting immune response against diverse types of tumors. To induce pyroptosis, we utilize designed tightly regulated gasdermin D variants comprising different pore-forming capabilities and diverse modes of activation, representing a toolbox of ICD inducers. We demonstrat...
Nature communications — 2024 — PMID 39737979
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