Peer-reviewed studies using the EVOS M5000 Imaging System for cancer cell imaging, proliferation, apoptosis, genome editing and tumour models. Each entry was discovered independently of Thermo Fisher's curated EVOS M5000 citations and verified to mention the EVOS M5000 Imaging System as a microscope in the full text. Paper cards link to Google Scholar, PubMed, PMC and DOI when available.
Cell type: epithelial | Technique: general | Disease/area: cancer | Molecular biology of the cell (2023)
The polarized distribution of membrane proteins into apical and basolateral domains provides the basis for specialized functions of epithelial tissues. The EGF receptor (EGFR) plays important roles in embryonic development, adult-epithelial tissue homeostasis,
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Cell type: T cell | Technique: EdU proliferation | Disease/area: cancer | Scientific reports (2023)
Notch signalling is one of the key molecular pathways involved in cell-to-cell signal transduction. Although the mechanisms of action of the NOTCH receptors are already relatively well known, their biological implications remain unclear, especially during the
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Cell type: macrophage | Technique: MTT | Disease/area: cancer, inflammation, wound healing | Frontiers in nutrition (2023)
Emulsifiers are extensively used as food additives and their consumption is increasing in Western countries. However, so far only few studies examined their potential effects on intestinal cellular functions and gut inflammation. The aim of this preliminary an
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Cell type: general | Technique: general | Disease/area: cancer, liver disease | Organogenesis (2023)
Primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC), the most common types of cholestatic liver disease (CLD), result in enterohepatic obstruction, bile acid accumulation, and hepatotoxicity. The mechanisms by which hepatocytes respond t
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Cell type: T cell, cancer cell | Technique: EdU proliferation | Disease/area: cancer | Life science alliance (2023)
Cancer cells make extensive use of the folate cycle to sustain increased anabolic metabolism. Multiple chemotherapeutic drugs interfere with the folate cycle, including methotrexate and 5-fluorouracil that are commonly applied for the treatment of leukemia and
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Cell type: cancer cell, cell line | Technique: EdU proliferation | Disease/area: cancer | Frontiers in oncology (2023)
Head and Neck Squamous Cell Carcinoma (HNSCC) remains a cancer with a poor prognosis, with a 5-year survival rate of less than 50%. Although epidermal growth factor receptor (EGFR) is almost always overexpressed, targeted anti-EGFR therapies have modest effica
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Cell type: endothelial | Technique: general | Disease/area: atherosclerosis, cancer, cardiovascular, inflammation | Frontiers in cardiovascular medicine (2023)
Atherosclerosis is a progressive disease that develops in areas of disturbed flow (d-flow). Progressive atherosclerosis is characterized by bulky plaques rich in mesenchymal cells and high-grade inflammation that can rupture leading to sudden cardiac death or
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Cell type: general | Technique: general | Disease/area: cancer | International journal of molecular sciences (2023)
Oral cancer is primarily squamous-cell carcinoma with a 5-year survival rate of approximately 50%. Lysyl oxidase (LOX) participates in collagen and elastin maturation. The propeptide of LOX is released as an 18 kDa protein (LOX-PP) in the extracellular environ
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Cell type: T cell, cancer cell, cell line | Technique: general | Disease/area: cancer | Nature communications (2023)
The testicular androgen biosynthesis is well understood, however, how cancer cells gauge dwindling androgen to dexterously initiate its de novo synthesis remained elusive. We uncover dual-phosphorylated form of sterol regulatory element-binding protein 1 (SREB
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Cell type: T cell, cancer cell, epithelial, fibroblast | Technique: confocal, immunofluorescence, live-cell imaging | Disease/area: cancer | Frontiers in immunology (2023)
Poor prognosis in colon cancer is associated with a high content of cancer-associated fibroblasts (CAFs) and an immunosuppressive tumor microenvironment. The relationship between these two features is incompletely understood. Here, we aimed to generate a model
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Cell type: general | Technique: CRISPR, EdU proliferation | Disease/area: cancer | Scientific reports (2023)
DNA methylation is important for establishing and maintaining cell identity and for genomic stability. This is achieved by regulating the accessibility of regulatory and transcriptional elements and the compaction of subtelomeric, centromeric, and other inacti
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Cell type: general | Technique: general | Disease/area: cancer | Frontiers in molecular biosciences (2023)
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide. Although many therapeutic options are available, several factors, including the presence of p53 mutations, impact tumor development and therapeutic resistance.
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Cell type: fibroblast | Technique: immunofluorescence | Disease/area: cancer | STAR protocols (2023)
Imaging organoid culture provides an excellent tool for studying complex diseases such as cancer. However, retaining the morphology of intact organoids for immunolabeling has been challenging. Here, we describe a protocol for immunofluorescence staining in int
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Cell type: T cell, cell line, tumor cell | Technique: general | Disease/area: cancer | Glia (2023)
Schwann cell-derived neoplasms known as malignant peripheral nerve sheath tumors (MPNSTs) are the most common malignancy and the leading cause of death in individuals with neurofibromatosis Type 1. Using genome-scale shRNA screens, we have previously found evi
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Cell type: cancer cell | Technique: general | Disease/area: cancer | Autophagy (2023)
The challenge of rapid macromolecular synthesis enforces the energy-hungry cancer cell mitochondria to switch their metabolic phenotypes, accomplished by activation of oncogenic tyrosine kinases. Precisely how kinase activity is directly exploited by cancer ce
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Cell type: general | Technique: general | Disease/area: cancer, diabetes | Nature communications (2022)
Pancreatic islet transplantation efficacy for type 1 diabetes (T1D) management is limited by hypoxia-related graft attrition and need for systemic immunosuppression. To overcome these challenges, we developed the Neovascularized Implantable Cell Homing and Enc
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Cell type: fibroblast | Technique: EdU proliferation | Disease/area: cancer, cardiovascular, diabetes, fibrosis, infectious disease | Biology direct (2022)
Cardiac fibrosis is a leading cause of cardiac dysfunction in patients with diabetes. However, the underlying mechanisms of cardiac fibrosis remain unclear. This study aimed to investigate the role of the long non-coding RNA (LncRNA) Airn in the pathogenesis o
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Cell type: macrophage | Technique: general | Disease/area: cancer, inflammation | Science advances (2022)
Chronic exposure to airborne carbon black ultrafine (nCB) particles generated from incomplete combustion of organic matter drives IL-17A-dependent emphysema. However, whether and how they alter the immune responses to lung cancer remains unknown. Here, we show
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Cell type: T cell | Technique: general | Disease/area: cancer | Nature communications (2022)
Solid tumours are highly refractory to immune checkpoint blockade (ICB) therapies due to the functional impairment of effector T cells and their inefficient trafficking to tumours. T-cell activation is negatively regulated by C-terminal Src kinase (CSK); howev
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Cell type: cancer cell | Technique: knockout | Disease/area: cancer | Open biology (2022)
Inhibition of respiratory complex I (CI) is becoming a promising anti-cancer strategy, encouraging the design and the use of inhibitors, whose mechanism of action, efficacy and specificity remain elusive. As CI is a central player of cellular bioenergetics, a
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Cell type: general | Technique: general | Disease/area: cancer, infectious disease | Frontiers in oncology (2022)
A critical risk factor for head and neck squamous cell carcinoma (HNSCC), particularly of the oropharynx, and the response to radiotherapy is human papillomavirus (HPV) type-16/18 infection. Specifically, HPV-positive HNSCC display increased radiosensitivity a
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Cell type: cell line | Technique: MTT | Disease/area: cancer | Turkish journal of pharmaceutical sciences (2022)
Colorectal cancer (CRC) remains a crucial health problem due to the toxicity of 5-Fluorouracil (5-FU) as first-line chemotherapy agent for treating CRC. The anticancer effects of boron and its compounds have been shown in various cell lines. This study aimed t
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Cell type: general | Technique: general | Disease/area: bone disease, cancer | Stem cells international (2022)
Cytogenetic aberrations may emerge in human mesenchymal stromal cells (MSC) during ex vivo expansion for cell therapy. We have detected clonal trisomy 5 in two distinct autologous MSC products expanded from bone marrow which, based on the current quality contr
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Cell type: epithelial | Technique: EdU proliferation, flow cytometry, immunocytochemistry | Disease/area: cancer, inflammation | Scientific reports (2022)
The small intestinal mucosa constitutes a physical barrier separating the gut lumen from sterile internal tissues. Junctional complexes between cells regulate transport across the barrier, preventing water loss and the entry of noxious molecules or pathogens.
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Cell type: cardiomyocyte, epithelial | Technique: EdU proliferation | Disease/area: cancer, inflammation | ACS applied materials & interfaces (2022)
Chemotherapy-induced cardiac toxicity is an undesirable yet very common effect that increases the risk of death and reduce the quality of life of individuals undergoing chemotherapy. However, no feasible methods and techniques are available to monitor and dete
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Cell type: general | Technique: general | Disease/area: cancer | RSC advances (2022)
Herein, we have introduced a series of half-sandwich Ru(ii)arene(N^N bpy/phen)-based RAPTA complexes for brain cancer therapy. Among all the synthesized complexes, [(η
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Cell type: cell line, epithelial | Technique: general | Disease/area: cancer | Journal for immunotherapy of cancer (2022)
Triple negative breast cancer (TNBC) is the most lethal subtype of breast cancer due to its aggressive behavior and frequent development of resistance to chemotherapy. Although natural killer (NK) cell-based immunotherapy is a promising strategy for overcoming
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Cell type: endothelial | Technique: EdU proliferation | Disease/area: cancer | NPJ microgravity (2022)
The advancement of microgravity simulators is helping many researchers better understanding the impact of the mechanically unloaded space environment on cellular function and disfunction. However, performing microgravity experiments on Earth, using simulators
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Cell type: cancer cell, cell line, epithelial | Technique: general | Disease/area: cancer, inflammation | Journal of extracellular vesicles (2022)
Although cancer-derived extracellular vesicles (cEVs) are thought to play a pivotal role in promoting cancer progression events, their precise effect on neighbouring normal cells is unknown. In this study, we investigated the impact of pancreatic cancer ductal
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Cell type: general | Technique: general | Disease/area: cancer | Journal of personalized medicine (2022)
New medical treatments are urgently needed for advanced hepatocellular carcinoma (HCC). Recently, we showed the anticancer effects of novel thiophene-based kinase inhibitors. In this study, we further characterized the antineoplastic effects and modes of actio
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Cell type: cancer cell | Technique: general | Disease/area: cancer | Cancers (2022)
Star-shaped hydrophobic porphyrins, acting as powerful fluorescent two-photon photosensitizers for oxygen in organic solvents, can easily be loaded into PMLABe polymeric nanoparticles at various concentrations. In this contribution, the performance of these po
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Cell type: cancer cell, cell line | Technique: general | Disease/area: cancer, inflammation | Biomedicines (2022)
Pancreatic cancer (PCa), one of the most malignant solid tumors, has a high mortality rate. Although there have been many trials of chemotherapeutic drugs such as gemcitabine, the mortality rates remain significantly higher than for other types of cancer. Ther
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Cell type: cancer cell, cell line | Technique: EdU proliferation | Disease/area: cancer, infectious disease | Molecular therapy oncolytics (2022)
Oncolytic vaccinia virus (OVV) has been reported to induce cell death in various types of cancer; however, the oncolytic activity of OVV in drug-resistant ovarian cancer remains limited. In the present study, we established doxorubicin-resistant ovarian cancer
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Cell type: MCF-7, T cell, cancer cell, cell line, tumor cell | Technique: general | Disease/area: cancer | Cell communication and signaling : CCS (2022)
The Notch signaling pathway is a crucial regulator of cell differentiation as well as tissue organization, whose deregulation is linked to the pathogenesis of different diseases. NOTCH1 plays a key role in breast cancer progression by increasing proliferation,
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Cell type: cancer cell, epithelial | Technique: general | Disease/area: cancer | Scientific reports (2022)
Anticancer strategies aimed at inhibiting Complex I of the mitochondrial respiratory chain are increasingly being attempted in solid tumors, as functional oxidative phosphorylation is vital for cancer cells. Using ovarian cancer as a model, we show that a comp
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Cell type: T cell, cancer cell | Technique: general | Disease/area: cancer | Nature chemical biology (2022)
Proteoglycans are heterogeneous macromolecular glycoconjugates that orchestrate many important cellular processes. While much attention has focused on the poly-sulfated glycosaminoglycan chains that decorate proteoglycans, other important elements of their arc
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Cell type: fibroblast, tumor cell | Technique: EdU proliferation | Disease/area: cancer, inflammation | Cell reports (2022)
Reduced p62 levels are associated with the induction of the cancer-associated fibroblast (CAF) phenotype, which promotes tumorigenesis in vitro and in vivo through inflammation and metabolic reprogramming. However, how p62 is downregulated in the stroma fibrob
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Cell type: epithelial | Technique: general | Disease/area: cancer | Cancers (2022)
The alteration of the cellular metabolism is a hallmark of glioma. The high glycolytic phenotype is a critical factor in the pathogenesis of high-grade glioma, including glioblastoma multiforme (GBM). GBM has been stratified into three subtypes as the proneura
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Cell type: general | Technique: general | Disease/area: cancer | Frontiers in oncology (2021)
Sarcomas are highly aggressive cancers of mesenchymal origin whose clinical management is highly complex. This is partly due to a lack of understanding of the molecular mechanisms underpinning the transformation of mesenchymal stromal/stem cells (MSCs) which a
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Cell type: T cell, cancer cell, cell line, tumor cell | Technique: GFP, immunocytochemistry | Disease/area: cancer | Cell communication and signaling : CCS (2021)
Loss of the Ras GTPase-activating protein neurofibromin promotes nervous system tumor pathogenesis in patients with neurofibromatosis type 1 (NF1). Neurofibromin loss potentially hyperactivates classic Ras (H-Ras, N-Ras, K-Ras), M-Ras, and R-Ras (R-Ras, R-Ras2
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Cell type: cell line | Technique: immunofluorescence, western blot | Disease/area: cancer | Aging (2021)
Propose: Autophagy plays a complicated role in cancer progression. This study aims at assessing the function of ATG5-induced autophagy in progression of lung squamous cell carcinoma and its upstream mechanism. TCGA database of lung squamous cell carcinoma was
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Cell type: cancer cell, cell line | Technique: general | Disease/area: cancer | American journal of cancer research (2021)
Successful treatment of advanced larynx squamous cell carcinoma (LSCC) remains a challenge, mainly due to limited response to chemotherapy and the phenomenon of the drug resistance. Therefore, new chemotherapeutic solutions are needed. The aim of this study wa
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Cell type: general | Technique: general | Disease/area: bone disease, cancer | Advanced functional materials (2021)
Nucleotide-based drugs, such as antisense oligonucleotides (ASOs), have unique advantages in treating human diseases as they provide virtually unlimited ability to target any gene. However, their clinical translation faces many challenges, one of which is poor
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Cell type: HeLa, cancer cell | Technique: fluorescence | Disease/area: cancer | PloS one (2021)
Multicolor fluorescence microscopy is a powerful technique to fully visualize many biological phenomena by acquiring images from different spectrum channels. This study expands the scope of multicolor fluorescence microscopy by serial imaging of polystyrene mi
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Cell type: T cell, cell line | Technique: flow cytometry, immunofluorescence | Disease/area: cancer | Nature communications (2021)
A major roadblock prohibiting effective cellular immunotherapy of pancreatic ductal adenocarcinoma (PDAC) is the lack of suitable tumor-specific antigens. To address this challenge, here we combine flow cytometry screenings, bioinformatic expression analyses a
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Cell type: cell line, epithelial, fibroblast | Technique: EdU proliferation | Disease/area: cancer, fibrosis | Aging (2021)
Prostate cell proliferation, driven by testosterone, is a major characteristic of benign prostatic hyperplasia (BPH). GV1001, a human telomerase reverse transcriptase catalytic subunit, is an injectable formulation used as a cancer vaccine. It functions as a c
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Cell type: tumor cell | Technique: general | Disease/area: cancer | Journal of enzyme inhibition and medicinal chemistry (2021)
Colorectal cancer (CRC) is the third leading cause of cancer-related deaths in men and in women. The impact of the new pyrazolo[4,3-
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Cell type: T cell, cancer cell, keratinocyte | Technique: general | Disease/area: cancer, infectious disease, inflammation, skin disease | Journal of microbiology and biotechnology (2021)
Prodigiosins, which are natural tripyrrole red pigments and synthetic derivatives, reportedly have multiple biological effects mainly on various types of cancer cells. However, the effects of bacterial prodigiosin on non-cancerous HaCaT human skin keratinocyte
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Cell type: HeLa, T cell | Technique: CRISPR, EdU proliferation | Disease/area: cancer | Molecular & cellular proteomics : MCP (2020)
The cell cycle is a highly conserved process involving the coordinated separation of a single cell into two daughter cells. To relate transcriptional regulation across the cell cycle with oscillatory changes in protein abundance and activity, we carried out a
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Cell type: HeLa, cancer cell | Technique: confocal | Disease/area: cancer | Cells (2019)
The Golgi apparatus undergoes disorganization in response to stress, but it is able to restore compact and perinuclear structure under recovery. This self-organization mechanism is significant for cellular homeostasis, but remains mostly elusive, as does the r
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