EVOS M5000 in Cancer Research: Independent Studies — Wave 2
Peer-reviewed cancer studies discovered outside Thermo Fisher's curated list. Each paper was discovered independently of Thermo Fisher's curated EVOS M5000 citations and verified to mention the EVOS M5000 Imaging System as a microscope in the full text. Paper cards link to Google Scholar, PubMed, PMC and DOI.
EVOS M5000CancerIndependentWave 2
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Design, Synthesis, and Bioactivity Assessment of Modified Vemurafenib Analog.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Melanoma
Background: Metastatic melanoma is a highly aggressive malignancy with poor prognoses and frequent resistance to conventional chemotherapy. Approximately 40% of melanoma cases carry the BRAFV600E mutation, for which vemurafenib, a selective BRAFV600E inhibitor, is approved. Despite initial clinical benefits, vemurafenib often leads to drug resistance and relapse, highlighting t
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Lipid transfer proteins and PI4KIIα initiate nuclear p53-phosphoinositide signaling.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cancer
EVOS M5000 microscope (<em>F</em>) and quantified based on the extracted dye using a plate reader (<em>G</em>). <em>p</em> value denotes ANOVA with Bonferroni's multiple comparisons test. Scale bar, 300 μm <em>
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Regulation of Ferroptosis in Human Macrophage by Nitric Oxide Donors.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Leukemia
Ferroptosis is a programmed form of cell death in which iron-dependent lipid peroxidation is the main feature. Macrophages are the major cells of the immune system, they function in a pro-oxidative environment, so the study of their susceptibility to ferroptosis and the search for approaches to its regulation are important. The aim of the study was to investigate ferroptosis in
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The MALAT1-EZH2 axis regulates PRC2 activity and promotes the mesenchymal phenotype in pediatric atypical teratoid/rhabdoid tumors.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cancer
BACKGROUND: Atypical teratoid/rhabdoid tumors (AT/RT) are aggressive pediatric CNS malignancies characterized by SMARCB1 loss, which leads to the dysregulated expression of Enhancer of Zeste Homolog 2 (EZH2), a key catalytic component of the Polycomb Repressive Complex 2 (PRC2). This dysregulation results in aberrant trimethylation of histone H3 at lysine 27 (H3K27me3), driving
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Deciphering the effects of bixin on pulmonary alveolar adenocarcinoma migration and proliferation via targeting BAX/BCL-2 and Cyclin D1.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Lung cancer
There is a tremendous upsurge in lung cancer incidences due to changing lifestyles and other environmental risk factors. Unfortunately, the use of clinical therapeutics is causing serious side effects and drug-resistant tumors. Taking account of the severity of lung cancer malignancy and the pressing need for natural therapeutics, we investigated the anticancer potential of bix
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Unraveling the therapeutic potential of Elaeagnus angustifolia extract on triple-negative breast cancer (TNBC): An investigation using zebrafish model.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Breast cancer
Breast cancer is a widespread and aggressive disease, with 2.3 million new cases globally in 2022. Metastasis, the spread of cancer cells to distant organs, remains a leading cause of breast cancer-related mortality. Current treatment options, particularly traditional chemotherapeutic drugs, are often associated with severe side effects, emphasizing the urgent need for safer an
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Ultra-Small Iron-Based Nanoparticles with Mild Photothermal-Enhanced Cascade Enzyme-Mimic Reactions for Tumor Therapy.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer
Chemodynamic therapy (CDT), which utilizes the catalytic reactions of nanoparticles to inhibit tumor growth, is a promising approach in cancer therapy. However, its efficacy is limited by insufficient hydrogen peroxide (H2O2) concentration in tumor microenvironments and unsatisfactory enzymatic catalytic activity. To overcome these limitations, ultra-small iron-based (USIB) nan
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Cholesterol confers resistance to Apatinib-mediated ferroptosis in gastric cancer.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cancer
BACKGROUND: Gastric cancer is the fifth leading cause of cancer-related deaths worldwide. Apatinib is a third-line treatment for gastric cancer. However, the development of resistance significantly limits its efficacy, and effective and safe strategies to overcome Apatinib resistance remain elusive. RESULTS: We found that Apatinib-resistant gastric cancer cells (MGC803/AR and A
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Low-intensity pulsed ultrasound mitigates acute extremity compartment syndrome by mediating ferroptosis suppression.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer
Acute extremity compartment syndrome (AECS) is a severe surgical emergency with no effective non-invasive therapies available. Here, we demonstrate that low-intensity pulsed ultrasound (LIPUS) attenuates muscle damage by suppressing oxidative stress and ferroptosis. In skeletal muscle cells following hypoxia-reoxygenation (H/R), LIPUS reduced reactive oxygen species (ROS), malo
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Serine 89 Phosphorylation Controls Nuclear Localization and Transcriptional Activity of ARID3B.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Ovarian cancer
Transcription factors that control stem cell programs are central drivers of cancer progression, metastasis, and therapy resistance. ARID3B, a DNA-binding protein overexpressed across multiple tumor types, expands the cancer stem cell population by regulating these pathways. Yet, how ARID3B is regulated remains largely unknown. Here, we uncover phosphorylation at Serine 89 as a
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An Open-Source Automated Pipeline for Quantitative Morphological Analysis of 3D-Bioprinted Cancer Cell Spheroids.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Breast cancer
Three-dimensional (3D) culture systems that recapitulate the tumor microenvironment are essential for studying cancer cell behavior, drug response, and cell-matrix interactions. Here, we present a detailed protocol for generating 3D spheroid cultures from murine breast cancer cells using methacrylated gelatin (GelMA)-based bioink and a CELLINK BioX bioprinter. This method enabl
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Circular RNA circNRIP1 promotes glioma progression by regulating the miR-106a-5p/GPR133 pathway.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Glioma
Glioma is one of the most common and aggressive types of brain tumors, characterized by generally low survival rates. Circular RNAs (circRNAs) have been identified as key players in the development of glioma. Our study aims to investigate the effect of circNRIP1 on glioma progression and elucidate the underlying mechanisms involved. The mRNA expression of circNRIP1, miR-106a-5p
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DLK1 is a GATA1s-driven dependency and therapeutic target in Down syndrome-associated myeloid leukemia.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Leukemia
EVOS M5000 imaging system (Thermo Fisher Scientific) in the transmitted light channel are shown. Scale bar, 100 μm. n = 3 biological replicates (right). (C) Growth curve showing the rise in the percentage of AM
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Immune checkpoint inhibitor-induced AMPA2 antibody-positive encephalitis: a case report.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Lung cancer
Immune checkpoint inhibitors (ICIs) have revolutionized cancer treatment but are associated with a range of immune-related adverse events, including neurological complications. We report the case of a 64-year-old man diagnosed with small-cell lung cancer who developed AMPA2 antibody-positive autoimmune encephalitis after receiving tislelizumab combined with chemotherapy. The pa
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Cannabidiol potentiates p53-driven autophagic cell death in non-small cell lung cancer following DNA damage: a novel synergistic approach beyond canonical pathways.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Lung cancer
EVOS M5000 imaging system (Invitrogen). The antibodies and the experiment conditions are listed in Supplementary Table <a href="#MOESM2" class="usa-link">1</a>.</p></section><section id="Sec12"><h3 class="pmc_s
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Composites of Reduced Graphene Oxide Based on Silver Nanoparticles and Their Effect on Breast Cancer Stem Cells.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Breast cancer
Graphene and its related nanocomposites have garnered significant interest due to their distinct physiochemical and biological properties. In this study, reduced graphene oxide-silver hybrid nanostructures were synthesized for applications in biomedical nanotechnology, particularly in targeting cancer stem cells (CSCs). A range of analytical techniques, such as X-ray diffractio
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Protective Effects of a New Human Placental Extract Against Hair Graying and Chemotherapy-Induced Peripheral Neuropathy.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Liver cancer
As stem cell therapy in regenerative medicine becomes more socially accepted, human perinatal tissue is attracting attention as a source because it can be harvested non-invasively. Human placental extracts (HPEs), which are prepared using acid hydrolysis and autoclaving, have been approved for treating menopausal disorders and liver dysfunction. This study investigated a new HP
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Amoebicidal effect of synthetic indoles against Acanthamoeba spp.: a study of cell death.
Cell type: Parasite | Technique: Fluorescence imaging | Disease/area: Cancer
Organic and synthetic chemistry plays a crucial role in drug discovery fields. Moreover, chemical modifications of available molecules to enhance their efficacy, selectivity and safety have been considered as an attractive approach for the development of new bioactive agents. Indoles, a versatile group of natural heterocyclic compounds, have been widely used in pharmaceutical i
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Cytosolic Immunostimulatory DNA Ligands and DNA Damage Activate the Integrated Stress Response, Stress Granule Formation, and Cytokine Production.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cancer
The presence of aberrant double-stranded DNA (dsDNA) in the cytoplasm of cells is sensed by unique pattern recognition receptors (PRRs) to trigger innate immune response. The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling pathway is activated by the presence of non-self or mislocalized self-dsDNA from nucleus or mitochondria released in response
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Expression of DNAJB1-PRKACA oncogene suppresses the differentiation potential of liver progenitor organoids towards a hepatocyte lineage.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Liver cancer
Fibrolamellar carcinoma (FLC) is a type of primary liver cancer that predominantly affects healthy adolescents and young adults in a background of normal liver. The DNAJB1-PRKACA fusion gene is an oncogenic driver in FLC tumors. To investigate the oncogenic mechanisms of this fusion gene, we developed a model using human liver progenitor organoids engineered to express DNAJB1-P
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Elesclomol-copper combination synergistically targets mitochondrial metabolism in cancer stem cells to overcome chemoresistance in PDAC.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Pancreatic cancer
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer with a poor prognosis, partly due to cancer stem cells (CSCs) that drive progression and treatment resistance. We explored the therapeutic potential of inducing cuproptosis, a copper-dependent, regulated cell death, in CSC-enriched PDAC models. Using human and murine PDAC models, we evaluated elesclomol, a copper t
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A key role for the exoribonuclease XRN1 in regulating the hepatitis B viral transcriptome.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Liver cancer
Chronic hepatitis B virus (HBV) is a leading cause of liver disease and hepatocellular carcinoma that persists as a DNA mini-chromosome and replicates via genomic RNA intermediates. While HBV replication has been extensively studied, mechanisms governing viral RNA degradation remain unclear. Here, we identify a key role for the 5'-3' exoribonuclease XRN1 in regulating the HBV t
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KNL1 Regulates Ferroptosis Resistance and Migration in Lung Adenocarcinoma Cells via AMPK-mTOR Signaling.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Lung cancer
Lung adenocarcinoma (LUAD), the most prevalent histological subtype of lung cancer, remains a leading cause of cancer-related mortality due to late diagnosis, metastasis, and therapy resistance. The aim of the study is to investigate the role of Kinetochore Scaffold 1 (KNL1) in promoting LUAD progression and its underlying molecular regulatory mechanisms.
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The miR-16-1-3p suppresses proliferation and invasiveness via the MDM2-p53 axis in TGF-β1 signaling in osteosarcoma.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cancer
The TGF-β signaling pathway has both tumor-suppressive and metastasis-promoting effects in cancer, yet the molecular determinants governing this switch remain unclear. Here, we investigated the miR-16-1-3p/MDM2/p53 axis as a potential regulator of TGF-β/Smad signaling output in osteosarcoma. miR-16-1-3p overexpression alone markedly reduced the proliferative and clonogenic pote
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Navigating the Collective: Nanoparticle-Assisted Identification of Leader Cancer Cells During Migration.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Breast cancer
Cancer-related deaths primarily occur due to metastasis, a process involving the migration and invasion of cancer cells. In most solid tumors, metastasis occurs through collective cell migration (CCM), guided by "cellular leaders". These leader cells generate forces through actomyosin-mediated protrusion and contractility. The cytoskeletal mechanisms employed by metastatic cell
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