EVOS M5000 in Cancer Research: Independent Peer-Reviewed Studies
Curated literature review of independent peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar, PubMed, PMC and DOI where available. Plankton & Zoom does not host paywalled content.
The EVOS M5000 Imaging System is widely adopted in oncology labs for brightfield and fluorescence read-outs of proliferation, migration, viability, and therapy response. The studies below were discovered independently of the manufacturer’s curated citation list and span glioma, breast, lung, ovarian, Ewing sarcoma, and veterinary mammary carcinoma models. These papers were identified by searching the open-access literature and verifying that the full text explicitly mentions the EVOS M5000 Imaging System as a microscope; they are not part of the manufacturer’s curated citation list.
Ferroptosis with Contributions from Apoptosis and Necroptosis in Porphyrazine III-Based Photodynamic Therapy of Primary Human Gliomas.
Cell type: Primary human glioma cells
Imaging technique: Fluorescence microscopy (photodynamic therapy)
Disease area: Glioma / brain cancer
Background: Photodynamic therapy (PDT) leading to immunogenic cell death (ICD) may serve as a promising basis for the development of antitumor therapeutic strategies. However, the mechanisms of action of photoinduced ICD in primary tumor cultures, including human glioma, remain unexplored. Methods: In the present study...
Pharmaceutics PMID 42357321 — PMC13305198
Google Scholar | PubMed | PMC | DOI
Genipin-Crosslinked, Silane-Anchored 3D Tumor-Stroma Microtissues for High-Content On-Chip Drug Testing.
Cell type: Tumor cells + cancer-associated fibroblasts (CAFs)
Imaging technique: 3D tumor-stroma microtissue / high-content on-chip imaging
Disease area: Cancer / drug resistance
Physiologically relevant 3D tumor models incorporating extracellular matrix (ECM) and cancer-associated fibroblasts (CAFs) are essential for studying tumor progression and drug resistance, yet often suffer from hydrogel contraction and instability-especially in microfluidic formats, where ECM deformation hampers long-t...
Adv Healthc Mater PMID 42121382 — PMC13307624
Google Scholar | PubMed | PMC | DOI
Redundancy of the OST catalytic subunit facilitates therapeutic targeting of N-glycosylation.
Cell type: Cancer cells / OST-engineered cell lines
Imaging technique: Fluorescence microscopy
Disease area: Cancer / N-glycosylation therapeutic targeting
Protein asparagine (N)-glycosylation, which promotes the folding and trafficking of cell surface receptors, has not traditionally been viewed as a viable target in oncology due to the essential and non-redundant enzymatic activities required for glycan synthesis and transfer. However, in mammals, an exception is the pr...
Cell Chem Biol PMID 40494352 — PMC13107401
Google Scholar | PubMed | PMC | DOI
Ionic regulation of cancer cell stiffness and metastatic colonization via the MRTFA-KCNMB1 axis.
Cell type: Cancer cells (breast / metastatic)
Imaging technique: Live-cell imaging of stiffness / ion channels
Disease area: Cancer metastasis
Cellular stiffness impacts multiple steps of cancer metastasis, but mechanisms that regulate the stiffness of cancer cells remain poorly understood. Here, we identified potassium efflux and potassium calcium-activated channel subfamily M regulatory beta subunit 1 (KCNMB1), an auxiliary subunit of the large conductance ...
Dev Cell PMID 42229427 — PMC13240663
Google Scholar | PubMed | PMC | DOI
TRIM26-mediated NKRF degradation drives Osimertinib resistance through SNRPD2-dependent stress granule formation in lung adenocarcinoma.
Cell type: Lung adenocarcinoma cells
Imaging technique: Fluorescence / live-cell imaging
Disease area: EGFR-mutant lung adenocarcinoma / osimertinib resistance
Osimertinib is the standard first-line therapy for EGFR-mutant lung adenocarcinoma; however, the inevitable development of acquired resistance leads to disease progression and treatment failure. While established resistance mechanisms primarily involve genetic alterations, stress-adaptive pathways, particularly stress ...
Cell Death Dis PMID 42026030 — PMC13237084
Google Scholar | PubMed | PMC | DOI
Protein kinase A regulates ferroptosis by controlling GPX4 m(6)A modification through phosphorylation of ALKBH5.
Cell type: Cancer cells
Imaging technique: Fluorescence microscopy (ferroptosis assays)
Disease area: Cancer
GPX4-dependent ferroptosis has emerged as a therapeutic strategy for cancer treatment. Here, we demonstrated that protein kinase A (PKA) participates in the regulation of ferroptosis by controlling the m6A modification of GPX4 in an ALKBH5-dependent manner. Notably, we identified ALKBH5, an m6A demethylase, as a novel ...
Cell Death Differ PMID 39901038 — PMC12163066
Google Scholar | PubMed | PMC | DOI
Single-cell transcriptomics reveals FXR1 as an actionable target for siRNA therapy in ovarian cancer.
Cell type: Ovarian cancer cells
Imaging technique: Imaging / single-cell transcriptomics validation
Disease area: Ovarian cancer
Ovarian cancer is one of the leading causes of cancer-related mortality among women and remains exceptionally difficult to manage and treat effectively in the clinic. Fragile X-related protein 1 (FXR1) is highly amplified and overexpressed in ovarian and several other cancers. FXR1 is a key regulator of the translation...
Nat Commun PMID 41932914 — PMC13219712
Google Scholar | PubMed | PMC | DOI
DNA replication stress and translational repression converge to drive CDK1- and caspase-dependent apoptosis in Ewing sarcoma.
Cell type: Ewing sarcoma cells
Imaging technique: Fluorescence / cell-death imaging
Disease area: Ewing sarcoma
Despite aggressive multimodal therapy, including cytotoxic chemotherapy, surgery, and radiation, the prognosis for patients with Ewing sarcoma remains poor, particularly for those with metastatic or relapsed disease. Combining agents that increase DNA replication stress with ATR-CHK1-WEE1 pathway inhibitors, which disr...
Oncogene PMID 42270776 — PMC13337492
Google Scholar | PubMed | PMC | DOI
HIF-1-regulated TPM3 links hypoxia to motility and invasion beyond the hypoxic fraction in triple-negative breast cancer.
Cell type: Triple-negative breast cancer cells
Imaging technique: Migration/invasion / live-cell imaging
Disease area: Triple-negative breast cancer
Hypoxia is a defining feature of triple-negative breast cancer (TNBC), driving invasion, metastasis, and therapy resistance. Understanding the molecular effectors of hypoxia is essential to identify new therapeutic targets. Here, we investigated tropomyosin 3 (TPM3), an actin-binding protein that regulates filament sta...
NPJ Breast Cancer PMID 41832172 — PMC13133141
Google Scholar | PubMed | PMC | DOI
Modulation of the endocannabinoid system reduces inflammatory signalling in canine mammary carcinoma cells.
Cell type: Canine mammary carcinoma cells
Imaging technique: Fluorescence / signaling imaging
Disease area: Canine mammary carcinoma / veterinary oncology
BACKGROUND: Canine mammary carcinoma (CMC) is characterised by a chronic inflammatory microenvironment resembling human breast cancer; however, the upstream regulatory mechanisms driving this phenotype remain unclear. The endocannabinoid system (ECS) has emerged as a potential modulator of inflammation and tumour biolo...
Vet Rec Open PMID 42078490 — PMC13128961
Google Scholar | PubMed | PMC | DOI