EVOS M5000 in Cardiovascular, Cell & Chemical Biology
Curated literature review of independent peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar, PubMed, PMC and DOI where available. Plankton & Zoom does not host paywalled content.
Beyond cell-line oncology, the EVOS M5000 is used for thrombosis modelling, ciliogenesis, yeast cell-factory engineering, fluorophore characterisation, mitochondrial studies in mussel cells, and C. elegans toxicology. These papers were identified independently. These papers were identified by searching the open-access literature and verifying that the full text explicitly mentions the EVOS M5000 Imaging System as a microscope; they are not part of the manufacturer’s curated citation list.
Coordinated roles of the CEP164 homodimer and TTBK2 are required for recruitment of the IFT machinery to the mother centriole for ciliogenesis.
Cell type: Ciliated cells / mother centriole / RPE1 cells
Imaging technique: Fluorescence microscopy
Disease area: Ciliopathy / ciliogenesis defects
Primary cilia are composed of axonemal microtubules that extend from the mother centriole-derived basal body and are sheathed by the ciliary membrane. Distal appendages (DAP) of the mother centriole play crucial roles as a scaffold to initiate ciliogenesis. Although previous studies indicated that the DAP proteins CEP1...
Mol Biol Cell PMID 40305080 — PMC12260181
Google Scholar | PubMed | PMC | DOI
Plg-R(KT) facilitates plasminogen incorporation and restrains thrombus growth under arterial shear in mice.
Cell type: Platelets / arterial thrombus
Imaging technique: Fluorescence/confocal imaging
Disease area: Thrombosis / cardiovascular
Platelets bind plasminogen facilitating surface-bound plasmin generation. We previously reported that the plasminogen receptor, Plg-RKT, retains plasminogen on activated platelets. Here, we investigate the significance of the interaction of Plg-RKT on thrombus formation, growth and stability. Whole blood from Plg-RKT-/...
Mol Med PMID 42129637 — PMC13339278
Google Scholar | PubMed | PMC | DOI
Unstable mitochondrial heteroplasmy in Mytilus edulis primary cell cultures.
Cell type: Mussel (Mytilus edulis) primary cells
Imaging technique: Fluorescence microscopy
Disease area: None — basic mitochondrial biology
Doubly uniparental inheritance is a phenomenon unique to bivalve mollusks characterized by the inheritance of mitochondria from both parents by male embryos and the maintenance of paternal mitochondria in male gonads. This lineage-specific mechanism has been described in several bivalve species, where male mitochondria...
PeerJ PMID 42405250 — PMC13333129
Google Scholar | PubMed | PMC | DOI
Solvatochromic Study of 2‑(N,N‑dimethyl)-3-alkynyl-pyridine Fluorophores.
Cell type: Fluorophore molecules / solvent systems
Imaging technique: Fluorescence microscopy / spectroscopy
Disease area: None — chemical probe development
A series of pyridine-based fluorophores2-(N,N-dimethyl)-3-alkynyl-pyridine (NNDAP), 4-[2-[2-(dimethylamino)-3-pyridyl]-ethynyl]-anisole (NNDAP-OMe), and 4-[2-[2-(dimethylamino)-3-pyridyl]-ethynyl]-benzonitrile (NNDAP-CN)were synthesized and their solvent-dependent photophysical properties were systematically investig...
ACS Omega PMID 42396038 — PMC13325342
Google Scholar | PubMed | PMC | DOI
Engineering chronological lifespan toward a robust yeast cell factory.
Cell type: Yeast cells (Saccharomyces cerevisiae)
Imaging technique: Brightfield/fluorescence imaging
Disease area: Industrial biotechnology / robust cell factories
Metabolic rewiring helps to construct efficient microbial cell factories; however, these cells suffer from metabolic stress during long-term fed-batch fermentation. Thus, the construction of robust cells is vital for industrial application of microbial cell factories at the laboratory scale. Here, we systematically cha...
Proc Natl Acad Sci U S A PMID 41213019 — PMC12646210
Google Scholar | PubMed | PMC | DOI
Human mitochondrial CYP2E1-mediated styrene metabolism increases oxidative stress and impairs antioxidant rescue in Caenorhabditis elegans.
Cell type: C. elegans / nematodes
Imaging technique: Fluorescence microscopy
Disease area: Styrene toxicity / oxidative stress
Styrene is an environmental toxicant metabolized by cytochrome P450 2E1 (CYP2E1) to styrene oxide, a reactive intermediate product linked to oxidative stress. While the role of CYP2E1 in xenobiotic metabolism is well established, the influence of subcellular enzyme localization on styrene-induced toxicity remains uncle...
Comp Biochem Physiol C Toxicol Pharmacol PMID 40780632 — PMC13156919
Google Scholar | PubMed | PMC | DOI