EVOS M5000 in Cardiovascular & Endothelial: Independent Studies — Wave 3

Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.

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Measuring Both pH and O

Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

<i>In vitro</i> studies which focus on cellular metabolism can benefit from time-resolved readouts from the living cells. pH and O₂ concentration are fundamental parameters upon which cellular metabolism is often inferred. This work demonstrates a novel use of a ruthenium oxide (RuO_<i>x</i>) electrode for <i>in vitro</i> studies. The RuO<i>_x</i>

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Lung on a Chip Development from Off-Stoichiometry Thiol-Ene Polymer.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Current in vitro models have significant limitations for new respiratory disease research and rapid drug repurposing. Lung on a chip (LOAC) technology offers a potential solution to these problems. However, these devices typically are fabricated from polydimethylsiloxane (PDMS), which has small hydrophobic molecule absorption, which hinders the application of this technology in

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Impact of chronic smoking on traumatic brain microvascular injury: An in vitro study.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Traumatic brain injury (TBI) is a major reason of cerebrovascular and neurological damage. Premorbid conditions such as tobacco smoking (TS) can worsen post-TBI injuries by promoting vascular endothelial impairments. Indeed, TS-induced oxidative stress (OS) and inflammation can hamper the blood-brain barrier (BBB) endothelium. This study evaluated the subsequence of chronic TS

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Modelling Metabolic Shifts during Cardiomyocyte Differentiation, Iron Deficiency and Transferrin Rescue Using Human Pluripotent Stem Cells.

Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Cardiomyocytes rely on specialised metabolism to meet the high energy demand of the heart. During heart development, metabolism matures and shifts from the predominant utilisation of glycolysis and glutamine oxidation towards lactate and fatty acid oxidation. Iron deficiency (ID) leads to cellular metabolism perturbations. However, the exact alterations in substrate metabolism

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IL-33 enhances Jagged1 mediated NOTCH1 intracellular domain (NICD) deubiquitination and pathological angiogenesis in proliferative retinopathy.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Pathological retinal neovascularization (NV) is a clinical manifestation of various proliferative retinopathies, and treatment of NV using anti-VEGF therapies is not selective, as it also impairs normal retinal vascular growth and function. Here, we show that genetic deletion or siRNA-mediated downregulation of IL-33 reduces pathological NV in a murine model of oxygen-induced r

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An

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Endothelial cells can acquire a mesenchymal phenotype in response to external stimuli through both mechanical and biological factors, using a process known as endothelial-to-mesenchymal (EndoMT) transition. EndoMT is characterized by the decrease in endothelial characteristics, increase in mesenchymal markers, and morphological changes. It has been recognized not only during de

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Detection of Anticancer Drug-Induced Cardiotoxicity Using VCAM1-Targeted Nanoprobes.

Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Chemotherapy-induced cardiac toxicity is an undesirable yet very common effect that increases the risk of death and reduce the quality of life of individuals undergoing chemotherapy. However, no feasible methods and techniques are available to monitor and detect the degree of cardiotoxicity at an early stage. Therefore, in this project, we aim to develop a fluorescent nanoprobe

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Wet adhesive hydrogel cardiac patch loaded with anti-oxidative, autophagy-regulating molecule capsules and MSCs for restoring infarcted myocardium.

Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Hydrogel patch-based stem cell transplantation and microenvironment-regulating drug delivery strategy is promising for the treatment of myocardial infarction (MI). However, the low retention of cells and drugs limits their therapeutic efficacies. Here, we propose a prefixed sponge carpet strategy, that is, aldehyde-dextran sponge (ODS) loading anti-oxidative/autophagy-regulatin

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Ablation of Shank3 alleviates cardiac dysfunction in aging mice by promoting CaMKII activation and Parkin-mediated mitophagy.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Compromised mitophagy and mitochondrial homeostasis are major contributors for the etiology of cardiac aging, although the precise underlying mechanisms remains elusive. Shank3, a heart-enriched protein, has recently been reported to regulate aging-related neurodegenerative diseases. This study aimed to examine the role of Shank3 in the pathogenesis of cardiac senescence and th

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Angiogenic adipokine C1q-TNF-related protein 9 ameliorates myocardial infarction via histone deacetylase 7-mediated MEF2 activation.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

C1q/tumor necrosis factor-related protein 9 (CTRP9) is an adipokine and has high potential as a therapeutic target. However, the role of CTRP9 in cardiovascular disease pathogenesis remains unclear. We found CTRP9 to induce HDAC7 and p38 MAPK phosphorylation via tight regulation of AMPK in vascular endothelial cells, leading to angiogenesis through increased

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Protective Role of Chronic Exercise Training in Modulating the Impact of Hyperglycemia on Vascular Sensitivity to Ischemia-Reperfusion.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Hyperglycemia (HG) is associated with increased mortality and morbidity in acute ischemic events. Regardless of the tissue or organs involved, the vascular endothelium is a key target of ischemia-reperfusion (I/R) injury severity. Among endothelium-protective strategies, exercise has been widely described as useful. However, whether this strategy is able to impact the deleterio

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Hypoxia Inhibits Cell Cycle Progression and Cell Proliferation in Brain Microvascular Endothelial Cells via the miR-212-3p/MCM2 Axis.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Hypoxia impairs blood-brain barrier (BBB) structure and function, causing pathophysiological changes in the context of stroke and high-altitude brain edema. Brain microvascular endothelial cells (BMECs) are major structural and functional elements of the BBB, and their exact role in hypoxia remains unknown. Here, we first deciphered the molecular events that occur in BMECs unde

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Spike Protein Impairs Mitochondrial Function in Human Cardiomyocytes: Mechanisms Underlying Cardiac Injury in COVID-19.

Cell type: Cardiomyocyte | Technique: Confocal / fluorescence | Disease/area: Cardiovascular & Endothelial

COVID-19 has a major impact on cardiovascular diseases and may lead to myocarditis or cardiac failure. The clove-like spike (S) protein of SARS-CoV-2 facilitates its transmission and pathogenesis. Cardiac mitochondria produce energy for key heart functions. We hypothesized that S1 would directly impair the functions of cardiomyocyte mitochondria, thus causing cardiac dysfunctio

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Sirtuin 6 Regulates the Activation of the ATP/Purinergic Axis in Endothelial Cells.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Sirtuin 6 (SIRT6) is a member of the mammalian NAD

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Improving retinal vascular endothelial cell tropism through rational rAAV capsid design.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Vascular endothelial cells (VEC) are essential for retinal homeostasis and their dysfunction underlies pathogenesis in diabetic retinopathy (DR) and exudative age-related macular degeneration (AMD). Studies have shown that recombinant adeno-associated virus (rAAV) vectors are effective at delivering new genetic material to neural and glial cells within the retina, but targeting

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Vascular cell-adhesion molecule 1 (VCAM-1) regulates JunB-mediated IL-8/CXCL1 expression and pathological neovascularization.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Vascular adhesion molecules play an important role in various immunological disorders, particularly in cancers. However, little is known regarding the role of these adhesion molecules in proliferative retinopathies. We observed that IL-33 regulates VCAM-1 expression in human retinal endothelial cells and that genetic deletion of IL-33 reduces hypoxia-induced VCAM-1 expression a

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Whey Improves In Vitro Endothelial Mitochondrial Function and Metabolic Redox Status in Diabetic State.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Endothelial dysfunction plays a critical role in the progression of type 2 diabetes mellitus (T2DM), leading to cardiovascular complications. Current preventive antioxidant strategies to reduce oxidative stress and improve mitochondrial function in T2DM highlight dietary interventions as a promising approach, stimulating the deepening of knowledge of food sources rich in bioact

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Senescence in human AC16 cardiac cells is associated with thymidine kinase induction and histone loss.

Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

AC16 cells are a transformed human cardiac cell line commonly used to study cardiomyocyte biology. We show that reduced proliferation and senescence markers can be robustly induced in AC16 cells cultured in low serum condition and treated with (i) low-dose doxorubicin, (ii) UV 254 nm, or (iii) H

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The interleukin-1 receptor type-1 in disturbed flow-induced endothelial mesenchymal activation.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Atherosclerosis is a progressive disease that develops in areas of disturbed flow (d-flow). Progressive atherosclerosis is characterized by bulky plaques rich in mesenchymal cells and high-grade inflammation that can rupture leading to sudden cardiac death or acute myocardial infarction. In response to d-flow, endothelial cells acquire a mesenchymal phenotype through endothelia

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Gelatin-Based Metamaterial Hydrogel Films with High Conformality for Ultra-Soft Tissue Monitoring.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Implantable hydrogel-based bioelectronics (IHB) can precisely monitor human health and diagnose diseases. However, achieving biodegradability, biocompatibility, and high conformality with soft tissues poses significant challenges for IHB. Gelatin is the most suitable candidate for IHB since it is a collagen hydrolysate and a substantial part of the extracellular matrix found na

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Embedded macrophages induce intravascular coagulation in 3D blood vessel-on-chip.

Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Macrophages are innate immune cells that prevent infections and help in wound healing and vascular inflammation. While these cells are natural helper cells, they also contribute to chronic diseases, e.g., by infiltrating the endothelial layer in early atherosclerosis and by promoting vascular inflammation. There is a crosstalk between inflammatory pathways and key players in th

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<i>Angelica gigas</i> extract inhibits acetylation of eNOS via IRE1α sulfonation/RIDD-SIRT1-mediated posttranslational modification in vascular dysfunction.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

<i>Angelica gigas</i> NAKAI (AG) is a popular traditional medicinal herb widely used to treat dyslipidemia owing to its antioxidant activity. Vascular disease is intimately linked to obesity-induced metabolic syndrome, and AG extract (AGE) shows beneficial effects on obesity-associated vascular dysfunction. However, the effectiveness of AGE against obesity and its underlying me

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Proteomic analysis reveals activation of platelet- and fibrosis-related pathways in hearts of ApoE

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Air pollution is an environmental risk factor linked to multiple human diseases including cardiovascular diseases (CVDs). While particulate matter (PM) emitted by diesel exhaust damages multiple organ systems, heart disease is one of the most severe pathologies affected by PM. However, the in vivo effects of diesel exhaust particles (DEP) on the heart and the molecular mechanis

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Sibjotang Protects against Cardiac Hypertrophy In Vitro and In Vivo.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Cardiac hypertrophy is developed by various diseases such as myocardial infarction, valve diseases, hypertension, and aortic stenosis. Sibjotang (, Shizaotang, SJT), a classic formula in Korean traditional medicine, has been shown to modulate the equilibrium of body fluids and blood pressure. This research study sought to explore the impact and underlying process of Sibjotang o

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Role of Argininosuccinate Synthase 1 -Dependent L-Arginine Biosynthesis in the Protective Effect of Endothelial Sirtuin 3 Against Atherosclerosis.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Atherosclerosis is initiated with endothelial cell (EC) dysfunction and vascular inflammation under hyperlipidemia. Sirtuin 3 (SIRT3) is a mitochondrial deacetylase. However, the specific role of endothelial SIRT3 during atherosclerosis remains poorly understood. The present study aims to study the role and mechanism of SIRT3 in EC function during atherosclerosis. Wild-type Sir

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The Antioxidant Properties of Glucosinolates in Cardiac Cells Are Independent of H

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

The organic sulfur-containing compounds glucosinolates (GSLs) and the novel gasotransmitter H

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Generation of a human iPSC-derived cardiomyocyte/fibroblast engineered heart tissue model.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Animal models have proven integral to broadening our understanding of complex cardiac diseases but have been hampered by significant species-dependent differences in cellular physiology. Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have shown great promise in the modelling of cardiac diseases despite limitations in functional and structural maturity. 3

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PIBF1 regulates trophoblast syncytialization and promotes cardiovascular development.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Proper placental development in early pregnancy ensures a positive outcome later on. The developmental relationship between the placenta and embryonic organs, such as the heart, is crucial for a normal pregnancy. However, the mechanism through which the placenta influences the development of embryonic organs remains unclear. Trophoblasts fuse to form multinucleated syncytiotrop

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Deletion of podocyte Rho-associated, coiled-coil-containing protein kinase 2 protects mice from focal segmental glomerulosclerosis.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Focal segmental glomerulosclerosis (FSGS) shares podocyte damage as an essential pathological finding. Several mechanisms underlying podocyte injury have been proposed, but many important questions remain. Rho-associated, coiled-coil-containing protein kinase 2 (ROCK2) is a serine/threonine kinase responsible for a wide array of cellular functions. We found that ROCK2 is activa

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MiR-148a-3p/SIRT7 Axis Relieves Inflammatory-Induced Endothelial Dysfunction.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

In endothelial cells, miR-148a-3p is involved in several pathological pathways, including chronic inflammatory conditions. However, the molecular mechanism of miR-148a-3p in endothelial inflammatory states is, to date, not fully elucidated. To this end, we investigated the involvement of miR-148a-3p in mitochondrial dysfunction and cell death pathways in human aortic endothelia

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Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus.

Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Diabetes mellitus (DM) is a progressive, chronic metabolic disorder characterized by high oxidative stress, which can lead to cardiac damage. Methionine sulfoxylation (MetO) of proteins by excessive reactive oxygen species (ROS) can impair the basic functionality of essential cellular proteins, contributing to heart failure. Methionine sulfoxide reductase B2 (MsrB2) can reverse

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Angiogenesis is limited by LIC1-mediated lysosomal trafficking.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Dynein cytoplasmic 1 light intermediate chain 1 (LIC1, DYNC1LI1) is a core subunit of the dynein motor complex. The LIC1 subunit also interacts with various cargo adaptors to regulate Rab-mediated endosomal recycling and lysosomal degradation. Defects in this gene are predicted to alter dynein motor function, Rab binding capabilities, and cytoplasmic cargo trafficking. Here, we

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CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Endothelial cell (EC)-pericyte interactions are known to remodel in response to hemodynamic forces; yet there is a lack of mechanistic understanding of the signaling pathways that underlie these events. Here, we have identified a novel signaling network regulated by blood flow in ECs-the chemokine receptor CXCR3 (CXC motif chemokine receptor 3) and one of its ligands, CXCL11 (C

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Sall4 and Gata4 induce cardiac fibroblast transition towards a partially multipotent state with cardiogenic potential.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Cardiac cellular fate transition holds remarkable promise for the treatment of ischemic heart disease. We report that overexpressing two transcription factors, Sall4 and Gata4, which play distinct and overlapping roles in both pluripotent stem cell reprogramming and embryonic heart development, induces a fraction of stem-like cells in rodent cardiac fibroblasts that exhibit unl

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Expression of Myeloperoxidase in Patient-Derived Endothelial Colony-Forming Cells-Associations with Coronary Artery Disease and Mitochondrial Function.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Myeloperoxidase (MPO) plays a critical role in the innate immune response and has been suggested to be a surrogate marker of oxidative stress and inflammation, with elevated levels implicated in cardiovascular diseases, such as atherosclerosis and heart failure, as well as in conditions like rheumatoid arthritis and cancer. While MPO is well-known in leukocytes, its expression

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Activation of a GPCR, ORL1 Receptor: A Novel Therapy to Prevent Heart Failure Progression.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

The number of ischemic heart failure (HF) patients is growing dramatically worldwide. However, there are at present no preventive treatments for HF. Our previous study showed that Gata4 overexpression improved cardiac function after myocardial infarction in rat hearts. We also found that Gata4 overexpression significantly increased the expression of a Pnoc gene, an endogenous l

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Endothelial cell Piezo1 promotes vascular smooth muscle cell differentiation on large arteries.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Vascular stabilization is a mechanosensitive process, in part driven by blood flow. Here, we demonstrate the involvement of the mechanosensitive ion channel, Piezo1, in promoting arterial accumulation of vascular smooth muscle cells (vSMCs) during zebrafish development. Using a series of small molecule antagonists or agonists to temporally regulate Piezo1 activity, we identifie

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An immunoregulatory and metabolism-improving injectable hydrogel for cardiac repair after myocardial infarction.

Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

The hypoxia microenvironment post-myocardial infarction (MI) critically disturbs cellular metabolism and inflammation response, leading to scarce bioenergy supplying, prolonged inflammatory phase and high risk of cardiac fibrosis during cardiac restoration. Herein, an injectable hydrogel is prepared by Schiff base reaction between fructose-1,6-bisphosphate (FBP)-grafted carboxy

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Characterizing SV40-hTERT Immortalized Human Lung Microvascular Endothelial Cells as Model System for Mechanical Stretch-Induced Lung Injury.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Drug development for human disease relies on preclinical model systems such as human cell cultures and animal experiments before therapeutic treatments can ultimately be tested on humans in clinical studies. We here describe the generation of a novel human cell line (HLMVEC/SVTERT289) that we generated by transfection of microvascular endothelial cells from healthy donor lung t

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17-beta estradiol prevents cardiac myocyte hypertrophy by regulating mitochondrial E3 ubiquitin ligase 1.

Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Cardiac hypertrophy is a cellular process characterized by the increased size of cardiomyocytes in response to a high workload or stress. 17-beta estradiol (E2) has cardioprotective and anti-hypertrophic effects by maintaining mitochondrial network and function. MUL1 is a mitochondrial ubiquitin ligase directly involved in the control of mitochondrial fission and mitophagy. Stu

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Compensatory lymphangiogenesis is required for edema resolution in zebrafish.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Edema, characterized by the accumulation of interstitial fluid, poses significant challenges in various pathological conditions. Lymphangiogenesis is critical in edema clearance, and delayed or inadequate lymphatic responses significantly hinder healing processes. However, real-time observation of dynamic changes in lymphangiogenesis during tissue repair in animal models has be

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Circulating Extracellular Vesicles as Putative Mediators of Cardiovascular Disease in Paediatric Chronic Kidney Disease.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Cardiovascular disease (CVD) is the leading cause of mortality in chronic kidney disease (CKD). However, the pathogenesis of CVD in CKD remains incompletely understood. Endothelial extracellular vesicles (EC-EVs) have previously been associated with CVD. We hypothesized that CKD alters EV release and cargo, subsequently promoting vascular remodelling. We recruited 94 children w

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A Disintegrin and Metalloproteinase 10 Regulates Ephrin B2-Mediated Endothelial Cell Sprouting and Ischemic Retinopathy.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Retinal neovascularization is the leading cause of visual impairment in diabetic retinopathy, retinopathy of prematurity, and age-related macular degeneration. The extracellular matrix breakdown by metalloproteinase leads to vascular complications in various proliferative retinopathies. A disintegrin and metalloproteinase domain-containing protein 10 (ADAM10) is involved in phy

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CCN2 Activates Cellular Senescence Leading to Kidney Fibrosis in Folic Acid-Induced Experimental Nephropathy.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Cellular communication network factor 2 (CCN2, also known as CTGF) is a complex protein that regulates numerous cellular functions. This biomolecule exhibits dual functions, depending on the context, and can act as a matricellular protein or as a growth factor. CCN2 is an established marker of fibrosis and a well-known mediator of kidney damage, involved in the regulation of in

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Micropatterning Photoconductive Peptide Assemblies on Stiff and Soft Biomaterial Substrates.

Cell type: Myocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

The propagation of electrical signals in the human heart relies on organized conduction pathways to optimally function and pump blood into the rest of the body. Mimicking this directionality across interconnected myocytes

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The nitrofen/bisdiamine murine model of congenital diaphragmatic hernia has a pulmonary hypertension vascular phenotype consistent with human CDH.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Congenital diaphragmatic hernia (CDH)-associated pulmonary hypertension (CDH-PH) has severe implications for the survival of patients with CDH; however, CDH-PH is often refractory to pulmonary vasodilators, rendering it difficult to treat. As such, models are necessary to study the etiology, mechanism, onset, and progression of pulmonary vascular remodeling in CDH. Despite seve

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High-throughput differentiation of human blood vessel organoids reveals overlapping and distinct functions of the cerebral cavernous malformation proteins.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Cerebral cavernous malformations (CCMs) are clusters of thin-walled enlarged blood vessels in the central nervous system that are prone to recurrent hemorrhage and can occur in both sporadic and familial forms. The familial form results from loss-of-function variants in the CCM1, CCM2, or CCM3 gene. Despite a better understanding of CCM pathogenesis in recent years, it is still

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Multi-stage cooperative ROS-responsive hydrogel platform for drug delivery in myocardial ischemia-reperfusion injury repair.

Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Myocardial ischemia-reperfusion (I/R) injury is characterized by oxidative stress, mitochondrial dysfunction, inflammation, and fibrosis, ultimately leading to chronic cardiac dysfunction and heart failure. Current therapeutic strategies that predominantly target single biological pathways exhibit limited long-term efficacy, underscoring the necessity for multi-targeted approac

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Xiong's Shiwei Wendan decoction attenuates plaque lesions and balances gut microbiota dysbiosis in ApoE-/- mice with high-fat diet.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

To evaluate the anti-atherosclerotic potential and gut microbiota (GM) modulation effects of Xiong's Shiwei Wendan decoction (, XSWD). For XSWD administration attenuated lipid deposition in THP-1 cells, significantly reduced aortic plaque lesions, improved the lipid profile, and normalized GM composition in HFD-fed ApoE-/- mice. This study investigated the potential anti-athero

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Decoding hippocampal subfield and glial responses in ischemia using single-cell transcriptomics.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Stroke affects more than 12 million individuals worldwide annually, leading to lasting physical and cognitive impairments. The peri-infarct environment in the central nervous system, comprising glial and blood vessel cells, contributes to stroke progression. The hippocampal CA1 region is particularly vulnerable to ischemia, whereas the adjacent CA3-DG region exhibits different

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3D modelling of pulmonary arterial stenosis and endothelial dysfunction in CTEPH.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Chronic thromboembolic pulmonary hypertension (CTEPH) arises from progressive thrombotic occlusion of pulmonary arteries, involving vessel blockage by unresolved thrombi and small vessel arteriopathy. This disrupts blood flow, increases lung pressure, and alters vessel geometry, contributing to endothelial dysfunction. However, the mechanisms remain unclear. To study these inte

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A hierarchical MgF

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Rare-earth-free magnesium (Mg) alloy bioresorbable stent (BRS) exhibits significant potential in vascular intervention due to its exceptional biosafety. However, its susceptibility to corrosion complicates surface functionalization and renders existing coating strategies ineffective for degradation-remodeling kinetics, resulting in delayed re-endothelialization and excessive lu

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Nao-Xin-Qing tablet inhibits macrophage inflammatory response in atherosclerosis via AMPK-α/SIRT1/PPAR-γ pathway.

Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Nao-Xin-Qing (NXQ) tablets are standardized proprietary herbal products containing an extract of Chinese persimmon leaves ( In vivo, the apolipoprotein E-deficient (ApoE In our study, we found that NXQ significantly reduced atherosclerotic plaques in the aortic root and aorta and decreased serum lipid levels in HFD-fed ApoE NXQ exerted a preventive effect on the development and

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Compound Tinglizi Decoction-Containing Serum Is Associated with Inhibition of PANoptosis in Cardiomyocytes via SIRT3-Related Chaperone-Mediated Autophagy of AIM2.

Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Septic cardiomyopathy (SCM) is a life-threatening complication of sepsis with no specific therapeutic options. Recent evidence suggests that PANoptosis, a programmed cell death pathway, contributes to myocardial injury in sepsis. Compound Tinglizi Decoction (CTLZC), a traditional Chinese herbal formula, has shown potential cardioprotective effects, yet the underlying biochemica

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Oscillatory Disturbed Flow Enhances Inflammatory and Oxidative Stress Markers in Endothelial Cells.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Hemodynamics significantly impact the biology of endothelial cells (ECs) lining the blood vessels. ECs are exposed to various hemodynamic forces, particularly frictional shear stress from flowing blood. While physiological flows are critical for the normal functioning of ECs, abnormal flow dynamics, known as disturbed flows, may trigger endothelial dysfunction leading to athero

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Systemic targeting of aberrant neovascular tufts using trehalose-dendrimer nanocarriers for the treatment of proliferative retinopathies.

Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

<b>Rationale:</b> Proliferative retinopathies are the leading causes of blindness worldwide. Current treatment paradigms rely heavily on intravitreal injections of anti-vascular endothelial growth factor A (anti-VEGFA) agents, which, despite their efficacy, are associated with ocular complications and patient discomfort. To address these challenges, we have developed a novel mi

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Multi-omics analysis reveals metabolic diversity underlying endothelial cell functions.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Endothelial cells (ECs) line the vascular system and are key players in vascular homeostasis, yet their metabolic diversity across tissues, vascular beds, and growth states remains poorly understood. This study examines metabolic differences between proliferating and quiescent ECs and compares blood and lymphatic endothelium using proteomics and metabolomics. Our findings indic

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Glutathione peroxidase mimic-engineered α-lactalbumin self-assembling coatings tailor immunomodulatory vascular stents for suppressing restenosis.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Timely endothelial regeneration and repair after stenting is essential for enhancing vascular healing performance. This necessitates that the stent surface be capable of maintaining a positive balance or sequential regulation among thrombosis, inflammation, and the growth behavior of smooth muscle cells (SMCs) and endothelial cells (ECs); however, a key challenge in achieving t

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Early Cytoskeletal Remodeling Drives Hypertrophic Cardiomyopathy Pathogenesis in

Cell type: Myocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Hypertrophic cardiomyopathy (HCM) is a common and deadly cardiac disease characterized by enlarged myocytes, increased myocardial wall thickening, and fibrosis. A majority of HCM cases are associated with mutations in the β-myosin heavy chain (

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Endoglin as a BMP9 co-receptor in vascular endothelial cells: prodomain displacement and TGFBRII recruitment.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Endoglin (ENG) is a single-pass transmembrane protein highly expressed in vascular endothelial cells (ECs), where it plays fundamental roles in EC functions. ENG is implicated in several cardiovascular disorders including hereditary haemorrhagic telangiectasia, pulmonary arterial hypertension (PAH) and preeclampsia. However, molecular mechanisms underlying ENG function are not

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PPARγ contributes to cardioprotection against heat stroke through ABCC5-dependent lipid metabolism.

Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Cardiac function has been found to be particularly vulnerable to climate change and temperature variability. However, the specific molecular mechanisms underlying the pathogenesis of heat stroke (HS)-induced myocardial dysfunction remain largely elusive. In this study, we constructed a cardiomyocyte-specific peroxisome proliferator-activated receptor γ (PPARγ) knockout mouse mo

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Leiomodin 1 haploinsufficiency promotes smooth muscle cell proliferation and increases neointimal lesion size.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

The transition of smooth muscle cells (SMCs) from a contractile to a synthetic phenotype is a key contributor to cardiovascular disease (CVD) pathologies, such as atherosclerosis and in-stent restenosis. We previously reported that loss of leiomodin 1 (LMOD1), a coronary artery disease risk gene highly expressed in SMCs, promotes SMC phenotypic switching in vitro. However, the

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The atypical E3 ligase HOIL-1 safeguards the ribosome during cellular stress.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

The ribosome has emerged as a signalling hub that can sense metabolic perturbations and coordinate responses that either restore homeostasis or initiate cell death. The range of insults that signal via the ribosome and the mechanisms governing such cell fate decisions remain uncharacterized. Here we identify the atypical E3 ligase HOIL-1 as an unexpected node in the ribosome si

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Kidney-Targeted Nanoparticle Delivery of Formoterol Mitigates Diabetic Kidney Disease without Adverse Cardiac Effects.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cardiovascular & Endothelial

Mitochondrial dysfunction plays a critical role in the progression of diabetic kidney disease (DKD). Previous research indicates that the FDA-approved β2-adrenergic receptor agonist and bronchodilator formoterol are efficacious at restoring mitochondrial function and slowing DKD progression. Unfortunately, β2-adrenergic receptor antagonists have been shown to result in cardiova

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