EVOS M5000 in Cell Biology & Signalling: Independent Studies — Wave 2

Cell death, organelles, signalling and mechanobiology with the EVOS M5000. Each paper was discovered independently of Thermo Fisher's curated EVOS M5000 citations and verified to mention the EVOS M5000 Imaging System as a microscope in the full text. Paper cards link to Google Scholar, PubMed, PMC and DOI.

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Fabrication of Aligned Polyhydroxybutyrate Fibrous Scaffolds via a Touchspinning Apparatus.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology

EVOS M5000) at 20×

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Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology

This study aimed to elucidate the pathogenesis of idiopathic gingival fibromatosis (IGF).

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Elucidation of Molecular Mechanisms of Lipid-Altered Cytotoxicity of TDP-43 Fibrils.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology

EVOS M5000 Imaging

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Low-Intensity Extracorporeal Shock Wave Therapy Alleviates Detrusor Muscle Apoptosis and Extracellular Matrix Dysregulation in the Bladder of Diabetic Rats.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology

Diabetic bladder dysfunction (DBD) affects 80% of diabetic patients, especially women. Yet, the management of DBD remains inconclusive. Building on our previous findings in animal models, low-intensity extracorporeal shock wave therapy (Li-ESWT) seems to be a promising potential therapy for DBD. However, the molecular mechanisms underlying the therapeutic effect of Li-ESWT on D

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Pulmonary delivery of siRNA lipoplexes and lipid nanoparticles using a vibrating mesh nebuliser.

Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cell Biology

Inhalation via nebulisation is a promising method to deliver high concentrations of siRNA to the lung epithelium in a direct and non-invasive manner for the treatment of numerous respiratory-related illnesses. However, nebulisation can be destructive towards siRNA nanocarriers leading to loss of siRNA and a diminished therapeutic outcome. Herein, we sought to explore how the ne

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Mechanical Reinforcement of Paper Biocomposites Using Filamentous Cyanobacteria.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology

EVOS M5000, Thermo Fisher,

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pH-dependent effects of pepsin and trypsin on the stability and antibiofilm functionality of pea protein-stabilized carvacrol nanoemulsions.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology

EVOS M5000 Imaging Microscope (Thermo Fisher Scientific, USA) at room temperature.</p></section><section id="s0040"><h3 class="pmc_sec_title">2.6. Apparent viscosity measurement</h3>

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Tuning tRNA synthetase inhibition reveals parabolic induction of stress granules limited in size and RNA content.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology

Translation elongation defects cause ribosome stalling and activate the integrated stress response (ISR). During the ISR, translation initiation suppression and ribosome runoff drive mRNA condensation into stress granules. However, the effects of partial translation elongation inhibition on stress granules are poorly defined. We demonstrate that intermediate levels of tRNA synt

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The Significant Antioxidant Effect Exerted by Pomegranate (Punica granatum): The Hidden Polyphenols.

Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Cell Biology

Background: Although the definition of dietary fibre is complex and constantly evolving, today we can identify it as "carbohydrate polymers with at least 10 monomeric units, which are not hydrolysed in the small intestine of humans". In addition to the numerous and well-known benefits of dietary fibre for human health, our attention is drawn to its antioxidant properties, achie

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TFEB overexpression alleviates autophagy-lysosomal deficits caused by progranulin insufficiency.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology

EVOS M5000 microscope (ThermoFisher). Four 20X z-stacks were taken from different areas of each coverslip.</p>

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Comparison of Culturing Methods of Primary Vaginal Fibroblasts.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cell Biology

EVOS M5000 microscope (Invitrogen, AMF5000, Waltman, MA) from day 0 until day 23 to capture cell morphology.</p></section><section id="S12"><h4 class="pmc_sec_title">Immunostaining western blot analysis:</h4>

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Systematic functional validation of IKAROS variants from patients and laboratory-generated mutations.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology

EVOS M5000 fluorescence microscope (40× objective). Scale bars, 25 μm. (B) EMSAs were performed using nuclear extracts from HEK293T cells transfected with the indicated IKAROS mutation alone or together with WT

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