EVOS M5000 in Cell Biology Research โ Wave 3
Independent papers using the EVOS M5000 Imaging System for cell lines, signalling, organelle imaging and general cell biology. Each entry was discovered independently of Thermo Fisher's curated EVOS M5000 citations and verified to mention the EVOS M5000 Imaging System as a microscope in the full text. Paper cards link to Google Scholar, PubMed, PMC and DOI when available.
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Genomic instability and mono-parental expression mitigate genomic shock in a cross-subgenus
Cell type: general | Technique: general | Disease/area: general | NAR molecular medicine (2026)
Hybridization, the merging of distinct genomes, is increasingly recognized as a major evolutionary force among eukaryotic pathogens, including facultatively sexual protist parasites like
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Graphene oxide-microplastic hybrid showcase elicited discrepancy through intrinsic interaction mediated steatosis, and apoptosis in macrophages.
Cell type: macrophage | Technique: EdU proliferation | Disease/area: general | Materials today. Bio (2026)
The widespread natural abundance of microplastics (MP) has been recognized to pose significant global health concerns, particularly due to limited understanding of their biological interactions. With the uncontrolled increase in MP accumulation in the environm
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Remote Ischemic Preconditioning Exerts Neuroprotective Effects Via the PGC-1ฮฑ/FNDC5/BDNF Pathway in Focal Brain Ischemia of Rats.
Cell type: neuron | Technique: EdU proliferation | Disease/area: general | Translational stroke research (2026)
Remote ischemic preconditioning (RIpreC) is a strategy for remotely protecting target organs such as the brain by applying brief ischemia and reperfusion to the limb. However, the mechanisms underlying RIpreC-induced neuroprotection remain unclear. We aimed to
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Structural characterization of recombinant humanized type III collagen cold gel and its effects on the biological functions of HaCaT cells.
Cell type: T cell | Technique: general | Disease/area: general | Frontiers in medicine (2026)
Recombinant humanized collagen has been widely used in the biomedical field. This study focuses on the characterization of a novel recombinant humanized type III collagen cold gel (C3Gel). To verify the structural integrity of C3Gel and explore its
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Prenatal Intraportal Delivery of Polymeric Nanoparticles to Fetal Rhesus Monkeys (
Cell type: general | Technique: general | Disease/area: general | Human gene therapy (2026)
Preliminary investigations focused on biodistribution of polymeric nanoparticles (NPs) shortly after ultrasound-guided delivery via the portal vein in early second-trimester fetal rhesus monkeys. Results demonstrated that poly(lactic-co-glycolic acid) (PLGA) N
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Evaluating Linker Architecture in RNA-Detecting Riboglow Probes and Effects on Fluorescence Turn-On.
Cell type: general | Technique: general | Disease/area: general | ACS chemical biology (2026)
Riboglow probes are small molecules where a synthetic fluorophore is connected to an RNA-binding moiety via a chemical linker. Upon binding a short RNA sequence, probe fluorescence intensity and lifetime increase. The fluorescence change is modulated by the ar
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STARTER: a stand-alone reconfigurable and translational organ-on-chip platform based on modularity and open design principles.
Cell type: general | Technique: general | Disease/area: general | Lab on a chip (2026)
Organ-on-chips (OoC) have the potential to revolutionize drug testing. However, the fragmented landscape of existing OoC systems leads to wasted resources and collaboration barriers, slowing broader adoption. To unite the ecosystem, there is an urgent need for
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Stress resilience is associated with transcriptional remodeling in the VTA.
Cell type: neuron | Technique: general | Disease/area: general | Cell reports (2026)
Individual responses to chronic stress vary, with some individuals remaining resilient while others exhibit susceptibility. Here, we use single-nucleus RNA sequencing to understand molecular adaptations in the ventral tegmental area (VTA) and lateral habenula
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CSF1R marks a subset of foetal haematopoietic multipotent progenitor cells with acute myeloid leukaemia propagation properties.
Cell type: T cell, cell line | Technique: general | Disease/area: general | Leukemia (2026)
KMT2A-rearranged infant leukaemia is one of the most severe malignancies in infants and children, and is characterised by a very aggressive phenotype and lineage plasticity. KMT2A::MLLT3 is among the most common translocations initiating leukaemia in infants,
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Nanoplastic toxicity and uptake in kidney cells: differential effects of concentration, particle size, and polymer type.
Cell type: cell line | Technique: EdU proliferation, flow cytometry | Disease/area: general | Cell biology and toxicology (2026)
Nanoplastics (NPs,โ<โ1 ยตm) are emerging environmental contaminants capable of crossing biological barriers and interacting at the cellular and subcellular level. Despite evidence of microplastics in human kidney tissue and urine, the renal effects of NPs re
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Monovalent pseudo-natural products supercharge degradation of IDO1 by its native E3 KLHDC3.
Cell type: general | Technique: general | Disease/area: general | Nature chemistry (2026)
Targeted protein degradation modulates protein function beyond the inhibition of enzyme activity or protein-protein interactions. Most degrader drugs function by directly mediating the proximity between a neosubstrate and a hijacked E3 ligase. Here we identify
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Nanoprecipitation and Drug Delivery with PMTC: Toward Biomedical Application of Polyesters from Radical Ring-Opening Polymerization.
Cell type: general | Technique: general | Disease/area: general | Macromolecular bioscience (2026)
Polymerized cyclic ketene acetals (PCKAs) prepared by radical ring-opening polymerization (RROP) have shown tremendous potential in the biomedical field. In this work, the field is expanded to the formation of fully degradable nanoparticles (NPs) from the fast
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Karyotypic Profiling of Induced Pluripotent Stem Cells Derived from a Xeroderma Pigmentosum Group C Patient.
Cell type: iPSC | Technique: general | Disease/area: general | Cells (2025)
Xeroderma Pigmentosum group C (XP-C) is an autosomal recessive disorder caused by mutations in the
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A microbiota-derived bile acid modulates biofilm formation by the probiotic strain Escherichia coli Nissle 1917.
Cell type: general | Technique: general | Disease/area: general | NPJ biofilms and microbiomes (2025)
Bacteria that colonize the human gut must withstand a variety of stressors, including detergent-like compounds known as bile acids. Here, we investigated how bile acids found in the human cecum and colon impact the behavior of the probiotic strain Escherichia
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Protocol for modeling injury-induced regeneration in retinal organoids and isolating hNRSCs for subretinal transplantation in rd10 mice.
Cell type: general | Technique: general | Disease/area: general | STAR protocols (2025)
Human retinal organoids (hROs) provide a platform for modeling injury and assessing regenerative capacity in vitro. Here, we present a protocol for stepwise generation of hROs from human embryonic stem cells (hESCs). We describe steps for microsurgical inducti
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Methanol biotransformation for the production of biodegradable plastic monomer L-lactate in yeast.
Cell type: general | Technique: general | Disease/area: general | Nature communications (2025)
Methanol is an ideal feedstock for biomanufacturing, and production of the biodegradable plastic monomer lactate from methanol is a promising approach for mitigating white pollution. However, it is challenging to engineer microbes for lactate production from m
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Synthesis of iron oxide quantum dots by non-thermal atmospheric pressure plasma electrolysis: influence of operating conditions and biomedical applications.
Cell type: general | Technique: general | Disease/area: general | RSC advances (2025)
Iron oxide quantum dots (IOQDs) were synthesized in this work using a distinct non-thermal atmospheric pressure plasma (NTAPP) electrolysis technique. The impact of the operational environment on the synthesis process was thoroughly investigated. The physicoch
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Exposure to the trichloroethylene metabolite S-(1,2-dichlorovinyl)-L-cysteine under hypoxic conditions (%O
Cell type: T cell, cell line | Technique: general | Disease/area: general | Toxicology letters (2025)
Trichloroethylene (TCE) is a volatile organic compound used as an industrial solvent until the recent EPA phaseout. Despite regulatory restrictions, legacy TCE pollution persists in soil and groundwater and poses a risk to human health. This chemical exerts it
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Fibroblast-loaded carboxymethyl chitosan-aldehyde hyaluronic acid injectable hydrogel for scleral remodelling to prevent development of myopia.
Cell type: fibroblast | Technique: general | Disease/area: general | Regenerative biomaterials (2025)
High myopia severely threatens the visual health of adolescents, with pathological features of decreased collagen aggregation and scleral thinning, ultimately leading to axial elongation on preretinal imaging. Fibroblasts play crucial roles in scleral remodell
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From Bench to Chairside: Collagen Scaffolds in Combination with Mesenchymal Stromal Cells for Gingival Augmentation.
Cell type: general | Technique: general | Disease/area: general | Dentistry journal (2025)
(Abstract unavailable)
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Iron overload-induced ferroptosis in CD8
Cell type: T cell | Technique: EdU proliferation, RT-qPCR, flow cytometry, immunofluorescence, immunohistochemistry, qPCR, western blot | Disease/area: general | BMC medicine (2025)
Endometriosis (EM) exhibits localised iron overload. However, the contribution of ferroptosis to EM pathogenesis remains unclear. We investigated how iron overload affects CD8โบ T cell immune function and the underlying ferroptotic mechanisms. We collected euto
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Targeted genome modification in protoplasts of a tea cultivar Kolkhida using RNA-guided Cas9 endonuclease.
Cell type: general | Technique: general | Disease/area: general | AoB PLANTS (2025)
Gene-editing tools enable precise, targeted genome modifications, providing new approach for the rapid and sustainable improvement of tea plant (
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Detection of electrical signals in fungal mycelia in response to external stimuli.
Cell type: general | Technique: general | Disease/area: general | iScience (2025)
Electrical signaling is a crucial mechanism for intercellular communication across diverse biological systems. Despite evidence of electrical activity in fungal mycelia, a standardized, reproducible method for detecting these signals is lacking. In this study,
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Rapalink-1 reveals TOR-dependent genes and an agmatinergic axis-based metabolic feedback regulating TOR activity and lifespan in fission yeast.
Cell type: general | Technique: general | Disease/area: general | Communications biology (2025)
The Target of Rapamycin, TOR, is a conserved signalling pathway with characterised chemical inhibitors such as rapamycin and torin1. Bi-steric third-generation inhibitors, such as rapalink-1 have been developed, however, their effects on organismal gene expres
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Synthesis of thiophene derived fluorescent sensor for Hg
Cell type: HeLa | Technique: general | Disease/area: general | Scientific reports (2025)
A new, simple, and robust thiol-based Schiff base ligand, (E)-3-(((2-mercaptophenyl) imino) methyl) pyridin-2-ol (PMP) as a chemical sensor for Hg
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Technique for decellularization and characterization of rat esophageal extracellular matrix for potential application as a 3D scaffold.
Cell type: general | Technique: general | Disease/area: general | Bioengineered (2025)
Conventional treatment for esophageal defects involves surgical removal of the defect area and implant conduit tissues. There exist morbidities and mortalities associated with the treatment including fistula and leakage leading to compromise in quality of life
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Quantitative Profiling of Nanoscopic Protein Aggregates Reveals Specific Fingerprint of TDP-43-Positive Assemblies in Motor Neuron Disease.
Cell type: neuron | Technique: fluorescence | Disease/area: general | Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2025)
Abnormal aggregation of TAR DNA-binding protein 43 (TDP-43) is a pathological hallmark of motor neuron disease (MND), yet current methods for quantifying these aggregates in biological samples remain limited in sensitivity and resolution. Here, single-molecule
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Shiga Toxin Type 2 Aggravates G1/S Phase Cell Cycle Arrest, Mediating Caspase-Independent Cell Death under Hyperosmotic Conditions in the Kidney.
Cell type: T cell, epithelial | Technique: general | Disease/area: general | Journal of microbiology and biotechnology (2025)
Shiga toxin (Stx) is a virulence factor produced by
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Cerebral Dopamine Neurotrophic Factor (CDNF) Acts as a Trophic Factor Promoting Neuritogenesis in the Dorsal Root Ganglia (DRG) Neurons Through Activation of the PI3K Signaling Pathway.
Cell type: neuron | Technique: general | Disease/area: general | Journal of neurochemistry (2025)
The cerebral dopamine neurotrophic factor (CDNF) is a neurotrophic factor extensively studied in the central nervous system because of its neuroprotective effects; however, its role in the peripheral nervous system (PNS) remains less explored. In this study, w
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Estrogenic regulation of perineuronal nets in the mouse insular cortex and hippocampus.
Cell type: neuron | Technique: qPCR | Disease/area: general | Neuropharmacology (2025)
Estrogen has profound effects on the brain, affecting neuronal plasticity and behavior. Perineuronal nets (PNNs) are perforated extracellular matrix structures that mostly surround parvalbumin (PV)-expressing inhibitory interneurons and regulate neuronal activ
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Commensal colonization of
Cell type: general | Technique: general | Disease/area: general | Microbiology spectrum (2025)
The ubiquitous fungal pathogen
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Deferoxamine prevents dexamethasone-induced muscle atrophy by reducing MuRF1 and atrogin-1.
Cell type: HeLa | Technique: EdU proliferation, immunocytochemistry, western blot | Disease/area: general | Frontiers in pharmacology (2025)
Muscle atrophy, commonly triggered by glucocorticoids such as dexamethasone (DEX), involves increased protein degradation via the ubiquitin-proteasome system. Recent findings suggest that iron imbalance can also induce muscle atrophy. However, there have been
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Facile Single-Nanocomposite 4D Bioprinting of Dynamic Hydrogel Constructs with Thickness-Controlled Gradient.
Cell type: general | Technique: general | Disease/area: general | Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2025)
The advent of 4D bioprinting has fueled significant progress in tissue engineering, but it faces major challenges such as limited options of smart bioinks and complexity of designing printing paths, limiting its broader application in tissue engineering. In th
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Mitochondrial transplantation: adaptive bio-enhancement.
Cell type: T cell | Technique: general | Disease/area: general | Cell death & disease (2025)
Mitochondria, often referred to the powerhouse of the cell, are essential for cellular energy production, and their dysfunction can profoundly affect various organs. Transplantation of healthy mitochondria can restore the bioenergetics in diseased cells and ad
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Clinical Scale MSC-Derived Extracellular Vesicles Enhance Poststroke Neuroplasticity in Rodents and Non-Human Primates.
Cell type: MSC, neuron | Technique: flow cytometry | Disease/area: general | Journal of extracellular vesicles (2025)
Stroke is a leading cause of death and disability. The therapeutic potential of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) has shown considerable promise in rodent models of stroke. However, the therapeutic efficacy and safety of clinical-s
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The dual-coding gene SLC35A4 protects against oxidative stress.
Cell type: general | Technique: general | Disease/area: general | Protein science : a publication of the Protein Society (2025)
Alternative proteins (AltProts) represent a newly recognized class of biologically active proteins encoded from alternative open reading frames (AltORFs) within already annotated genes. This study focuses on the SLC35A4 gene, which encodes both the reference p
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Cholinergic neuronal activity promotes diffuse midline glioma growth through muscarinic signaling.
Cell type: neuron | Technique: EdU proliferation | Disease/area: general | Cell (2025)
Glutamatergic neuronal activity promotes proliferation of both oligodendrocyte precursor cells (OPCs) and gliomas, including diffuse midline glioma (DMG). However, the role of neuromodulatory brainstem neurons projecting to midline structures where DMGs arise
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Sex hormone-binding globulin controls sex-specific lipolytic activity in human abdominal subcutaneous adipocytes.
Cell type: adipocyte | Technique: general | Disease/area: general | Molecular metabolism (2025)
Regulation of lipid metabolism is fundamental for metabolic health, and adipose tissue is a central component in this process. Adipose tissue differs considerably between women and men in terms of a higher subcutaneous capacity for storage, which is linked to
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Muscle stem cells in Duchenne muscular dystrophy exhibit molecular impairments and altered cell fate trajectories impacting regenerative capacity.
Cell type: general | Technique: general | Disease/area: general | Cell death & disease (2025)
Satellite cells are muscle-resident stem cells that maintain and repair muscle. Increasing evidence supports the contributing role of satellite cells in Duchenne muscular dystrophy (DMD), a lethal degenerative muscle disease caused by loss of dystrophin. Howev
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The potent neuroepithelium-promoting activity of Otx2 during gastrulation, as demonstrated by its exogenous epiblast-wide expression in chicken embryos.
Cell type: T cell | Technique: general | Disease/area: general | Frontiers in cell and developmental biology (2025)
Although Otx2 has been a topic of research for over 3 decades, the role of Otx2 expressed in the brain-forming anterior epiblast during gastrulation has not been clarified. We focused on epiblast regions where Otx2 expression is absent or downregulated, namely
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Harnessing cell size to separate genetically and functionally distinct dental pulp-derived mesenchymal stromal cell subpopulations.
Cell type: T cell, macrophage | Technique: general | Disease/area: general | Journal of biological engineering (2025)
BACKGROUND: Dental pulp-derived mesenchymal stromal cells (DPMSCs) represent a promising avenue for regenerative medicine. However, the therapeutic potency of DPMSCs is substantially influenced by their functional heterogeneity emerging during ex vivo expansio
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The nitrofen/bisdiamine murine model of congenital diaphragmatic hernia has a pulmonary hypertension vascular phenotype consistent with human CDH.
Cell type: general | Technique: general | Disease/area: general | American journal of physiology. Lung cellular and molecular physiology (2025)
Congenital diaphragmatic hernia (CDH)-associated pulmonary hypertension (CDH-PH) has severe implications for the survival of patients with CDH; however, CDH-PH is often refractory to pulmonary vasodilators, rendering it difficult to treat. As such, models are
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Liver portal fibroblasts induce the functions of primary human hepatocytes in vitro.
Cell type: fibroblast | Technique: EdU proliferation | Disease/area: general | Communications biology (2025)
In vitro human liver models are critical to mitigate species-specific differences observed for toxicology, disease modeling, and regenerative medicine. Interactions with mesenchyme (i.e., fibroblasts) can promote phenotypic functions of primary human hepatocyt
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RXFP2-positive mesenchymal stem cells in the antlerogenic periosteum contribute to postnatal development of deer antlers.
Cell type: MSC, macrophage | Technique: EdU proliferation | Disease/area: general | Communications biology (2025)
The postnatal development of secondary sexual characteristics is a highly complex process governed by diverse molecular signals and serves as a key marker of sexual maturity. Deer antlers exemplify such traits, distinguished not only by their unique ability to
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Integrated analysis of molecular atlases unveils modules driving developmental cell subtype specification in the human cortex.
Cell type: neuron | Technique: general | Disease/area: general | Nature neuroscience (2025)
Human brain development requires generating diverse cell types, a process explored by single-cell transcriptomics. Through parallel meta-analyses of the human cortex in development (seven datasets) and adulthood (16 datasets), we generated over 500 gene co-exp
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Protocol for extraction and characterization of mouse brain-derived extracellular matrix for neuronal cell culture.
Cell type: neuron | Technique: general | Disease/area: general | STAR protocols (2025)
Neuronal cell cultures are highly sensitive to their microenvironment, particularly the choice of coating substrate. Here, we present a protocol to isolate extracellular matrix (ECM) from mouse brain tissue, providing a native coating material that closely mim
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Characterization of the
Cell type: neuron | Technique: general | Disease/area: general | FASEB bioAdvances (2025)
In vivo cell type-specific genetic recombination based on the Cre-loxP system has contributed to the understanding of biological processes and diseases. Neuronal nuclei (NeuN)/RBFOX3 is a widely used mature neuron marker in developmental biology and neuroscien
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Ultrasound-assisted H
Cell type: general | Technique: general | Disease/area: general | Frontiers in pharmacology (2025)
Schizophyllan (SPG), a bioactive polysaccharide from This study developed an ultrasound-assisted hydrogen peroxide (H This study demonstrates that ultrasound-assisted H
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Select autosomal dominant DFNA11 deafness variants activate Myo7A targeting in epithelial cells.
Cell type: epithelial | Technique: EdU proliferation | Disease/area: general | Journal of cell science (2025)
Myosin-7A (Myo7A) is a motor protein crucial for the organization and function of stereocilia, specialized actin-rich protrusions on the surface of inner ear hair cells that mediate hearing. Variants in Myo7A cause several forms of genetic hearing loss, includ
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3D spheroid model reveals enhanced efficacy of mannose-decorated nanoparticles for TB treatment.
Cell type: macrophage | Technique: general | Disease/area: general | Nanomedicine (London, England) (2025)
Tuberculosis (TB), caused by We explored a combination therapy using bedaquiline and pretomanid encapsulated in polymeric nanoparticles (pNPs). Further, active targeting was achieved through mannose-decorated nanoparticles (Man-pNPs) for macrophage-specific d
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Light-Intensity-Dependent Control of Collagen Hydrogel Properties via Riboflavin Phosphate-Mediated Photocrosslinking.
Cell type: fibroblast | Technique: general | Disease/area: general | Materials (Basel, Switzerland) (2025)
While photocrosslinked collagen hydrogels show promise in tissue engineering, conventional approaches for property control often require complex chemical modifications or concentration changes that alter their biochemical composition. Here, we present the firs
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Wild Birds' Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem Cells.
Cell type: fibroblast | Technique: EdU proliferation, immunohistochemistry | Disease/area: general | Methods and protocols (2025)
Follicular cells represent a valuable resource for genetic research, biotechnology and cryopreservation in biobanks, particularly for the conservation of endangered species. They offer a more practical alternative to gametes, embryos and fibroblasts. Collectio
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Reactive oxygen species attenuation improves the quality of vitrified-warmed bovine embryos.
Cell type: general | Technique: general | Disease/area: general | Animal reproduction (2025)
The aim of this study was to investigate the effects of modulating reactive oxygen species (ROS) in vitrified bovine in vitro produced (IVP) embryos. In experiment I we compared ROS production in fresh and vitrified-warmed blastocysts. In experiment II we eval
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The coiled-coil protein carrier structure affects the activation of certain endocytosis pathways.
Cell type: general | Technique: general | Disease/area: general | RSC advances (2025)
Coiled-coil protein carrier (CCPC) 140 is a rigid and anisotropically structured cationic coiled-coil artificial protein that has displayed up to a 1000 times higher level of cellular internalization activity than that of unstructured cell-penetrating peptides
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Generation of induced pluripotent stem cell lines TRNDi037-A and TRNDi038-A from two patients with Alagille syndrome carrying heterozygous JAG1 mutations.
Cell type: cell line, iPSC | Technique: general | Disease/area: general | Stem cell research (2025)
Human induced pluripotent stem cell (iPSC) lines TRNDi037-A and TRNDi038-A were generated from the lymphoblastoid cell lines (LCL) of two patients with different heterozygous JAG1 variants resulting in Alagille syndrome (ALGS). ALGS is a rare genetic disease o
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