EVOS M5000 in Imaging Technique Development — Wave 3
Independent papers highlighting EVOS M5000 imaging techniques, fluorescence, live-cell and high-content methods. Each entry was discovered independently of Thermo Fisher's curated EVOS M5000 citations and verified to mention the EVOS M5000 Imaging System as a microscope in the full text. Paper cards link to Google Scholar, PubMed, PMC and DOI when available.
EVOS M5000IndependentWave 3
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Single-cell exon deletion profiling reveals splicing events that shape gene expression and cell state dynamics.
Cell type: general | Technique: CRISPR | Disease/area: general | Nature communications (2026)
Alternative splicing is a pervasive gene regulatory mechanism critical for diversifying the human proteome. To systematically investigate its role in cell fate determination, we develop scCHyMErA-Seq, a scalable CRISPR-based exon deletion screening platform in
Google Scholar | PubMed | PMC | DOI
Morphological and Molecular Characterization of Apple Scab (
Cell type: general | Technique: EdU proliferation | Disease/area: general | Current issues in molecular biology (2025)
Apple scab, caused by the fungus
Google Scholar | PubMed | PMC | DOI
(Title unavailable)
Cell type: general | Technique: EdU proliferation | Disease/area: general | International journal of molecular sciences (2025)
Current therapies for Primary Amoebic Meningoencephalitis (PAM) present several limitations; consequently, there is an urgent need to develop new therapeutic agents. In this regard, we undertook bioassay-guided isolation of
Google Scholar | PubMed | PMC | DOI
Postnatal alterations in skeletal muscle fiber composition of myostatin-knockout chickens.
Cell type: general | Technique: CRISPR, EdU proliferation, immunofluorescence, knockout | Disease/area: general | Poultry science (2025)
In a previous study, we identified a hypermuscular phenotype attributed to both muscle fiber hyperplasia and hypertrophy in myostatin (MSTN)-knockout (KO) chickens generated by clustered regularly interspaced short palindromic repeats-associated protein 9 (CRI
Google Scholar | PubMed | PMC | DOI
Temporal effects of sympathetic denervation on aortic remodeling and rupture in experimental abdominal aortic aneurysm.
Cell type: general | Technique: knockout | Disease/area: general | American journal of physiology. Heart and circulatory physiology (2025)
Abdominal aortic aneurysm (AAA) is a life-threatening condition with no effective pharmacologic therapies, and increasing evidence suggests that the sympathetic nervous system (SNS) contributes to disease progression. However, the temporal effects of SNS modul
Google Scholar | PubMed | PMC | DOI
Protease Enzyme Supplementation in Weaning Piglets Fed Reduced Crude Protein Diets: Effects on Gut Health Integrity and Performance Response.
Cell type: general | Technique: EdU proliferation | Disease/area: general | Animals : an open access journal from MDPI (2025)
Two trials evaluated the effects of dietary protease inclusion in weaned piglets fed diets with or without crude protein (CP) reduction, focusing on performance, intestinal health, and amino acid digestibility. In Trial I, 270 piglets (21-63 days) received six
Google Scholar | PubMed | PMC | DOI
Xiong's Shiwei Wendan decoction attenuates plaque lesions and balances gut microbiota dysbiosis in ApoE-/- mice with high-fat diet.
Cell type: general | Technique: EdU proliferation, knockout | Disease/area: atherosclerosis | Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan (2025)
To evaluate the anti-atherosclerotic potential and gut microbiota (GM) modulation effects of Xiong's Shiwei Wendan decoction (, XSWD). For XSWD administration attenuated lipid deposition in THP-1 cells, significantly reduced aortic plaque lesions, improv
Google Scholar | PubMed | PMC | DOI
Effects of heterozygous SMG1 mutations on nonsense-mediated mRNA decay in human pluripotent stem cell model.
Cell type: general | Technique: EdU proliferation | Disease/area: general | Molecules and cells (2025)
Nonsense-mediated mRNA decay (NMD) eliminates transcripts containing premature termination codons, thereby preventing errors in protein synthesis. Serine/threonine-protein kinase SMG1 is a crucial kinase for NMD response, interacting with other regulatory prot
Google Scholar | PubMed | PMC | DOI
Micropatterning Photoconductive Peptide Assemblies on Stiff and Soft Biomaterial Substrates.
Cell type: general | Technique: EdU proliferation | Disease/area: general | ACS applied materials & interfaces (2025)
The propagation of electrical signals in the human heart relies on organized conduction pathways to optimally function and pump blood into the rest of the body. Mimicking this directionality across interconnected myocytes
Google Scholar | PubMed | PMC | DOI
Ribosome association inhibits stress-induced gene mRNA localization to stress granules.
Cell type: general | Technique: EdU proliferation | Disease/area: general | Genes & development (2025)
The integrated stress response (ISR) is critical for resilience to stress and is implicated in numerous diseases. During the ISR, translation is repressed, stress-induced genes are expressed, and mRNAs condense into stress granules. The relationship between st
Google Scholar | PubMed | PMC | DOI
LRG1 inhibition promotes acute pancreatitis recovery by inducing cholecystokinin Type 1 receptor expression via Akt.
Cell type: general | Technique: knockout | Disease/area: general | Theranostics (2025)
(Abstract unavailable)
Google Scholar | PubMed | PMC | DOI
Dielectrophoretic Microfluidic Designs for Precision Cell Enrichments and Highly Viable Label-Free Bacteria Recovery from Blood.
Cell type: general | Technique: EdU proliferation | Disease/area: general | Micromachines (2025)
Conducting detailed cellular analysis of complex biological samples poses challenges in cell sorting and recovery for downstream analysis. Label-free microfluidics provide a promising solution for these complex applications. In this work, we investigate partic
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Late gestation maternal overnutrition alters gene expression and histomorphology in neonatal foal testes.
Cell type: general | Technique: EdU proliferation | Disease/area: general | Journal of animal science (2025)
Reproductive health of male offspring following perturbations in maternal nutrition is not well-described in the horse; therefore, the objective of this study was to determine the effects of maternal overnutrition on neonatal foal testicular development. Sixte
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Selenium Broussonetia papyrifera polysaccharide alleviated cyclophosphamide-induced immune suppression, growth inhibition, intestinal damage, and gut microbiota disorder in yellow-feather broilers.
Cell type: general | Technique: EdU proliferation | Disease/area: general | Poultry science (2025)
This study aims to investigate the effects of selenium Broussonetia papyrifera polysaccharide (Se-BPP) on growth performance, immune regulation, intestinal barrier function, and gut microbiota in cyclophosphamide (CTX)-induced immunosuppressed chicks. A total
Google Scholar | PubMed | PMC | DOI
Simultaneous aerobic and anaerobic respiration in hot spring chemolithotrophic bacteria.
Cell type: general | Technique: EdU proliferation | Disease/area: general | Nature communications (2025)
Aerobic and anaerobic organisms and their functions are spatially or temporally decoupled at scales ranging from individual cells to ecosystems and from minutes to hours. This is due to competition for energy substrates and/or biochemical incompatibility with
Google Scholar | PubMed | PMC | DOI
FAM43A coordinates mtDNA replication and mitochondrial biogenesis in response to mtDNA depletion.
Cell type: general | Technique: EdU proliferation | Disease/area: general | The Journal of cell biology (2025)
Mitochondrial retrograde signaling (MRS) pathways relay the functional status of mitochondria to elicit homeostatic or adaptive changes in nuclear gene expression. Budding yeast have "intergenomic signaling" pathways that sense the amount of mitochon
Google Scholar | PubMed | PMC | DOI
Engineering Long-Releasing Hollow-like or Condensed Progesterone Hormone Microcrystals with Controlled Polymorphism.
Cell type: general | Technique: brightfield | Disease/area: general | Small science (2024)
Progesterone is an endogenous steroid hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. Progesterone crystallization techniques have previously reported. Among these techniques, solvent crystallization and diffe
Google Scholar | PubMed | PMC | DOI
Mitochondrial epigenetics brings new perspectives on doubly uniparental inheritance in bivalves.
Cell type: general | Technique: EdU proliferation | Disease/area: general | Scientific reports (2024)
Mitochondrial epigenetics, particularly mtDNA methylation, is a flourishing field of research. MtDNA methylation appears to play multiple roles, including regulating mitochondrial transcription, cell metabolism and mitochondrial inheritance. In animals, bivalv
Google Scholar | PubMed | PMC | DOI
GluN3A and Excitatory Glycine Receptors in the Adult Hippocampus.
Cell type: general | Technique: knockout | Disease/area: general | The Journal of neuroscience : the official journal of the Society for Neuroscience (2024)
The GluN3A subunit of
Google Scholar | PubMed | PMC | DOI
Phytochemical Profiling and Biological Activities of Pericarps and Seeds Reveal the Controversy on "Enucleation" or "Nucleus-Retaining" of
Cell type: general | Technique: EdU proliferation | Disease/area: general | Molecules (Basel, Switzerland) (2024)
The fruits of
Google Scholar | PubMed | PMC | DOI
Deletion of podocyte Rho-associated, coiled-coil-containing protein kinase 2 protects mice from focal segmental glomerulosclerosis.
Cell type: general | Technique: knockout | Disease/area: general | Communications biology (2024)
Focal segmental glomerulosclerosis (FSGS) shares podocyte damage as an essential pathological finding. Several mechanisms underlying podocyte injury have been proposed, but many important questions remain. Rho-associated, coiled-coil-containing protein kinase
Google Scholar | PubMed | PMC | DOI
Mitochondrial Transplantation Therapy Ameliorates Muscular Dystrophy in
Cell type: general | Technique: MTT | Disease/area: general | Biomolecules (2024)
Duchenne muscular dystrophy is caused by loss of the dystrophin protein. This pathology is accompanied by mitochondrial dysfunction contributing to muscle fiber instability. It is known that mitochondria-targeted in vivo therapy mitigates pathology and improve
Google Scholar | PubMed | PMC | DOI
Effect of sulfasalazine on ferroptosis during intestinal injury in rats after liver transplantation.
Cell type: general | Technique: western blot | Disease/area: general | Scientific reports (2024)
Using a rat autologous orthotopic liver transplantation (AOLT) model and liver cold ischemia-reperfusion (I/R)-induced intestinal injury, we clarified whether ferroptosis occurred in rat AOLT cold I/R-induced intestinal injury. Additionally, the role and possi
Google Scholar | PubMed | PMC | DOI
Modular vector assembly enables rapid assessment of emerging CRISPR technologies.
Cell type: general | Technique: CRISPR | Disease/area: general | Cell genomics (2024)
The diversity of CRISPR systems, coupled with scientific ingenuity, has led to an explosion of applications; however, to test newly described innovations in their model systems, researchers typically embark on cumbersome, one-off cloning projects to generate c
Google Scholar | PubMed | PMC | DOI
Lycopene Maintains Mitochondrial Homeostasis to Counteract the Enterotoxicity of Deoxynivalenol.
Cell type: general | Technique: EdU proliferation | Disease/area: general | Antioxidants (Basel, Switzerland) (2023)
The intestinal tract is a target organ for Deoxynivalenol (DON) absorption and toxicity. Mitochondrial homeostasis imbalance is the gut toxicity mechanism of DON. Lycopene (LYC) has intestinal protective effects and can maintain mitochondrial homeostasis in re
Google Scholar | PubMed | PMC | DOI
Effect of Fusidic Acid and Some Nitrogen-Containing Derivatives on Liposomal and Mitochondrial Membranes.
Cell type: general | Technique: EdU proliferation | Disease/area: general | Membranes (2023)
The paper assesses the membranotropic action of the natural antibiotic fusidic acid (FA) and its derivatives. It was found that a FA analogue with ethylenediamine moiety (derivative 2), in contrast to native FA and 3,11-dioxime analogue (derivative 1), is able
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Conditional, Tissue-Specific CRISPR/Cas9 Vector System in Zebrafish Reveals the Role of Nrp1b in Heart Regeneration.
Cell type: general | Technique: CRISPR | Disease/area: general | Arteriosclerosis, thrombosis, and vascular biology (2023)
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeat-associated 9) technology-mediated genome editing has significantly improved the targeted inactivation of genes in vitro and in vivo
Google Scholar | PubMed | PMC | DOI
Marine Meroterpenoids Isolated from
Cell type: general | Technique: EdU proliferation | Disease/area: general | Pharmaceuticals (Basel, Switzerland) (2023)
(Abstract unavailable)
Google Scholar | PubMed | PMC | DOI
The
Cell type: general | Technique: EdU proliferation | Disease/area: general | Microorganisms (2023)
Fungal plant pathogens can present major problems for most crop species. Currently, control of fungal diseases relies heavily on the use of fungicides. However, there are problems associated with fungicide use, including potential toxicity to non-target organi
Google Scholar | PubMed | PMC | DOI
MAX: a simple, affordable, and rapid tissue clearing reagent for 3D imaging of wide variety of biological specimens.
Cell type: general | Technique: EdU proliferation | Disease/area: general | Scientific reports (2022)
Transparency of biological specimens is crucial to obtaining detailed 3-dimensional images and understanding the structure and function of biological specimens. This transparency or tissue clearing can be achieved by adjusting the refractive index (RI) with em
Google Scholar | PubMed | PMC | DOI
Comparative Efficiency for in vitro Transfection of Goat Undifferentiated Spermatogonia Using Lipofectamine Reagents and Electroporation.
Cell type: general | Technique: GFP, transfection | Disease/area: general | Stem cells and cloning : advances and applications (2022)
Spermatogonial stem cells (SSC), also referred to as undifferentiated spermatogonia, are the germline stem cells responsible for continuous spermatogenesis throughout a male's life. They are, therefore, an ideal target for gene editing. Previously, SSC fr
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A New Protocol of Computer-Assisted Image Analysis Highlights the Presence of Hemocytes in the Regenerating Cephalic Tentacles of Adult
Cell type: general | Technique: immunohistochemistry | Disease/area: general | International journal of molecular sciences (2021)
In humans, injuries and diseases can result in irreversible tissue or organ loss. This well-known fact has prompted several basic studies on organisms capable of adult regeneration, such as amphibians, bony fish, and invertebrates. These studies have provided
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