EVOS M5000 in Immunology, Infection & Host-Pathogen Research — Wave 2
Host-pathogen, antimicrobial and immune-cell imaging with the EVOS M5000. Each paper was discovered independently of Thermo Fisher's curated EVOS M5000 citations and verified to mention the EVOS M5000 Imaging System as a microscope in the full text. Paper cards link to Google Scholar, PubMed, PMC and DOI.
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Caspofungin-Based Red-Emissive Probes for Fluorescent Imaging of Pathogenic Fungi.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Fungal infection
Fungal infections are an often-neglected cause of morbidity worldwide, while their inaccurate and often inconclusive diagnosis indirectly promotes high morbidity and the development of antimicrobial resistance. Here, the clinically used semisynthetic echinocandin antifungal caspofungin was selectively conjugated to red-emitting fluorescent dyes to develop new optical imaging pr
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Bioprospecting for Anti-Kinetoplastid Drug Discovery from Aloysia citrodora Essential Oil.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Immunology
Natural products have long been recognized as invaluable resources in drug discovery. Essential oils have attracted widespread attention due to their broad spectrum of biological activities. Herein, we report the anti-kinetoplastid activity of Aloysia citrodora leaf essential oil through a bioassay-guided fractionation method against the etiological agents of Chagas disease and
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Liquid-liquid phase inclusion bodies in acute and persistent parainfluenaza virus type 5 infections.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Viral infection
Cytoplasmic inclusion bodies (IBs) are a common feature of single-stranded, non-segmented, negative-strand RNA virus (Mononegavirales) infections and are thought to be regions of active virus transcription and replication. Here we followed the dynamics of IB formation and maintenance in cells infected with persistent and lytic/acute variants of the paramyxovirus, parainfluenza
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Identification of a novel monoclonal antibody derived from the lumpy skin disease virus ORF123 that confers cross-binding and cross-neutralizing activity against Capripoxvirus members.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Immunology
Capripoxviruses cause diseases (e.g., lumpy skin disease, sheep pox, and goat pox) that significantly hinder the growth of livestock production in endemic areas. Here, we systemically describe a B-cell monoclonal antibody (mAb) derived from the lumpy skin disease virus (LSDV) ORF123, which exhibits cross-reactivity with goat poxvirus (GTPV) and sheep poxvirus (SPPV). A novel co
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Mechanism Insight of Cell Death Signaling by Thymol Derivatives on Trypanosomatidae Protozoan Parasites.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Parasitic infection
Leishmaniasis and Chagas disease are parasitic diseases considered to be among the most important neglected diseases, with implications for both developed and developing countries. Currently, there are no effective therapeutic treatments for these diseases due to challenges in drug administration, high toxicity, high costs, and drug resistance. In this study, a series of eleven
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A novel antiviral strategy targeting human metapneumovirus through pH modulation in human airway epithelial cells.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Viral infection
Human metapneumovirus (hMPV) is a major cause of respiratory infections, particularly in vulnerable populations, yet no approved vaccines or targeted antivirals are available. pH-regulated processes, including airway epithelial physiology, endosomal acidification and viral fusion mediated by the fusion (F) protein, are critical for hMPV infection. This study evaluates PHOH-001,
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In vitro biocontrol potential of plant extract-based formulation against infection structures of Phytophthora infestans along with lower non-target effects.
Cell type: Bacterial | Technique: Fluorescence imaging | Disease/area: Bacterial infection
Late blight, caused by Phytophthora infestans, is among the most destructive diseases affecting tomatoes and potatoes. The use of synthetic fungicides is becoming increasingly restricted due to the banning of several active ingredients for environmental and health reasons. Moreover, the rise of fungicide-resistant strains is compromising their effectiveness. Solutions for susta
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Alloferon and IL-22 receptor expression regulation on the pathogenesis of imiquimod-induced psoriasis.
Cell type: Keratinocyte | Technique: Fluorescence imaging | Disease/area: Immunology
Psoriasis is an immune-mediated inflammatory skin disease. IL-22, a proinflammatory cytokine, is implicated in psoriasis pathogenesis; however, there is currently no established biological treatment targeting IL-22 or its receptor, IL-22Rα. Alloferon is a short peptide that has an antiinflammatory effect on skin disorders; however, little is known about its anti-inflammatory ac
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B10 cells promote pro-resolving macrophage function through direct cell-cell contact and IL-10 secretion in Raw 264.7 cells.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Immunology
It is well known that regulatory B cells (Breg), especially IL-10-producing regulatory cells (B10), play an important role in immune regulation during inflammatory and infectious diseases. Although it has been revealed that the immune regulatory function of B10 can be exerted through cognate cell-cell contact with T cells, more research is needed to delineate its impact on othe
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Amoebicidal Effect of COVID Box Molecules against Acanthamoeba: A Study of Cell Death.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Immunology
Acanthamoeba spp. can cause a sight threatening disease. At present, the current treatments used to treat Acanthamoeba spp. Infections, such as biguanide-based antimicrobials, remain inefficacious, with the appearance of resistant forms and high cytotoxicity to host cells. In this study, an initial screening was conducted against Acanthamoeba castellanii Neff and murine macroph
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Nelfb promotes dermal white adipose tissue formation through RNA polymerase II-mediated adipogenic gene regulation.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Viral infection
Dermal white adipose tissue (dWAT) is crucial for skin homeostasis, contributing to hair follicle regeneration, immune defense and skin wound healing. dWAT is formed and maintained by the differentiation of adipocyte precursors found in the dermis of the skin. While transcription factors that control adipocyte differentiation have been well characterized, other aspects of trans
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The Combination of Curcumae Radix and Syzygium Aromaticum Extracts Mitigates Benign Prostatic Hyperplasia through Anti-Proliferative and Anti-Inflammatory Effects.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Immunology
Benign prostatic hyperplasia (BPH) is a urinary tract disorder that primarily affects geriatric males. Natural materials have been developed to treat and prevent symptoms of BPH. However, a few natural functional foods has been conclusively proven to cure or prevent symptoms of BPH. The study aimed to investigate the anti-proliferative mechanism of Curcumae Radix (CR) and Syzyg
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Isoimperatorin alleviates fungal keratitis by regulating NF-κB pathway and macrophage immune response.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Fungal infection
The purpose of this research study was to investigate the impact of isoimperatorin on Aspergillus fumigatus (A. fumigatus) keratitis and elucidate its underlying mechanisms via the TLR4/MyD88/IKK/NF-κB signaling pathway.
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Cyclodextrin Counteracts Coxsackievirus-Induced Cardiac Damage by Protecting Desmosome Integrity and Suppressing Proinflammatory Cytokine Expression.
Cell type: Myocyte | Technique: Fluorescence imaging | Disease/area: Viral infection
Nuclear factor of activated T cells 5 (NFAT5), an osmosensitive transcription factor, has been shown to protect against coxsackievirus B3 (CVB3)-induced myocarditis but is susceptible to cleavage by viral proteases. Identifying agents that upregulate NFAT5 may offer a novel antiviral strategy. Cyclodextrins, cyclic oligosaccharides that influence cellular osmolality, are promis
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Cardiac Cell Membrane-Coated Nanoparticles as a Potential Targeted Delivery System for Cardiac Therapy.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Immunology
Cardiomyopathies, a cause of heart failure, are a predominant cause of death globally and may lead to discernible myocardial abnormalities. Several therapeutic agents were discovered, developed, investigated, and evaluated to save patients' lives and improve their quality of life. The effective administration of drugs improves therapeutic outcomes while reducing side effects. N
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SPCS2 serves as a critical host factor for JEV replication by regulating viral protein stability and virion assembly.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Viral infection
Evos m5000). The fluorescence-positive ratio of cells was calculated using the ImageJ software.</p></section><section id="s4-6"><h3 class="pmc_sec_title">RNA interference</h3>
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Alloferon regulates the growth and movement of Trichomonas vaginalis by altering hydrogenosomes.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Viral infection
Trichomoniasis infected with Trichomonas vaginalis (T. vaginalis), can cause mild symptoms like itching and burning but can also lead to more serious adverse outcomes. While typically treated with metronidazole, this medication can face resistance from T. vaginalis and some individuals may experience side effects. Hence, the research on effective therapeutic methods is essentia
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Probiotics Ameliorate Histological Alterations and Prevent Increases in Cytokine and Toxin Levels in Mice Infected with Various Clostridioides difficile Strains.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Immunology
EVOS M5000 Imaging System microscope (Thermo Fisher Scientific, Waltham, MA, USA) and examined by two blinded pathologists, who graded enteritis using the following scoring system: epithelial cell damage (score
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Phragmites australis (Cav.) Trin. ex Steud. Extract Induces Apoptosis-like Programmed Cell Death in Acanthamoeba castellanii Trophozoites.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Immunology
Acanthamoeba keratitis (AK) is an infectious ocular disease which is difficult to diagnose correctly and cure. Development of an effective and safe therapeutic drug for AK is needed. Our preliminary screening of more than 200 extracts from wild plants collected in Korea suggested the potential amoebicidal activity of Phragmites australis (Cav.) Trin. ex Steud. extract (PAE) aga
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A Human-Based Skin-Lymphoreticular Model-on-Chip to Emulate Inflammatory Skin Conditions.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Immunology
Despite fast-paced advances in bioengineering human-based models, emulating fine-tuned and interdependent immunoregulations of the human body remains challenging. While many bioengineered models contain immune cells, secondary lymphoid tissues are typically missing. Here, we report a self-assembled, minimally functional lymphoreticular unit composed of fibroblastic reticular, l
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Brassinin Induces H2S Signals and Improves Vascular Smooth Muscle Cell Functions.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Immunology
Brassinin, a sulfur-containing phytoalexin, exerts anticancer and anti-inflammatory effects. Hydrogen sulfide (H2S) is an important gasotransmitter with significant cardioprotective properties. The effects of brassinin on H2S signaling and vascular smooth muscle cell (SMC) functions remain unexplored. This study found that brassinin protected against angiotensin II (Ang II)-ind
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Genetic analysis of pigment production in the fungus Exophiala dermatitidis mutant strains obtained via nontargeted UV mutagenesis.
Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Immunology
Exophiala dermatitidis is a polyextremotolerant black yeast species. E. dermatitidis produces 1,8-dihydroxynaphthalene (DHN) melanin via the polyketide synthase 1 (PKS1) pathway enabling it to survive harmful conditions. This study focused on random nontargeted mutagenesis to obtain albino (alb) and hyperpigmented (hyp) mutants. Notably, all 17 alb mutants possessed mutations i
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In Vitro Modulation of Murine Tenocyte Behavior by Hyperbaric Oxygen Therapy.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Immunology
Tendon injuries often result in prolonged healing and excessive scar tissue formation due to chronic hypoxia in this poorly perfused tissue. Hyperbaric oxygen therapy (HBOT), a non-invasive treatment currently used for chronic wounds, may improve tendon healing by relieving hypoxia. However, the effects of HBOT on tendon cells, particularly tenocytes, remain poorly understood.
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A multicellular vessel-on-a-chip model reveals context-dependent roles for platelets in inflammation and inflammatory hemostasis.
Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Immunology
The immune system identifies and destroys invading pathogens. However, chronic inflammation and excessive tissue damage can occur if inflammation is not resolved or if the immune system misidentifies a threat. Therefore, the immune system must be tightly controlled. However, the complex cell-cell and cell-microenvironment interactions that regulate immune-cell recruitment are c
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Understanding Heterogeneity and Tolerance of Dual Candida albicans-Staphylococcus aureus Biofilms to Cold Atmospheric Plasma and Antimicrobial Combinations.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Immunology
EVOS M5000 fluorescent microscope using a DAPI filter (Ex./Em. 357/447 nm). Calcofluor white has a similar optimum Ex. (380 nm) and Em. (475 nm) spectrum to DAPI, making the filter suitable for imaging.</p></se
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Investigating Bacterial Vaginosis Pathogenesis Using Peptide Nucleic Acid-Fluorescence In Situ Hybridization With a Focus on the Roles of Gardnerella Species, Prevotella bivia, and Fannyhessea vaginae.
Cell type: Bacterial | Technique: Fluorescence imaging | Disease/area: Bacterial infection
Bacterial vaginosis (BV) is a vaginal dysbiosis characterized by polymicrobial communities of BV-associated bacteria (BVAB) adhered to the vaginal epithelium. Despite decades of research, its etiology remains unknown. We aimed to investigate BV biofilm formation over time among women who developed incident BV (iBV) using peptide nucleic acid-fluorescence in situ hybridization (
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A reporter Oropouche virus expressing ZsGreen from the M segment enables pathogenesis studies in mice.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Viral infection
Oropouche fever caused by Oropouche virus (OROV) is a significant zoonosis in Central and South America. Despite its public health significance, we lack high-throughput diagnostics, therapeutics, and a comprehensive knowledge of OROV biology. Reporter viruses are valuable tools to rapidly study virus dynamics and develop neutralization and antiviral screening assays. OROV is a
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Neurokinin-1 receptor activation protects against cardiac fibrosis, inflammation and diastolic dysfunction in type 2 diabetic mice.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Immunology
The pathogenesis of type 2 diabetes mellitus (T2DM)-induced cardiomyopathy involves cardiac fibrosis that leads to diastolic dysfunction. We established that replacement of lost substance P (SP) that occurs in T2DM reduces cardiac fibrosis and decreases inflammation in T2DM mice and non-human primates. This study aimed to identify the specific anti-fibrotic SP receptor.
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Recovery of phenotypically sorted cells using droplet-digital microfluidics.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Immunology
Droplet microfluidics has become a ubiquitous and powerful tool for high-throughput phenotypic screening at the single-cell level. Large numbers of cells can be sorted for a variety of functions, including the secretion of antibodies with tailored properties. The recovery of cells from sorted droplets is still very poor compared to droplet sorting, usually being limited to arou
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Uropathogenic Escherichia coli Biofilms: Antibiotic Pressure and Interaction with Human Neutrophils.
Cell type: Bacterial | Technique: Fluorescence imaging | Disease/area: Bacterial infection
Uropathogenic Escherichia coli (UPEC) is a primary cause of urinary tract infections (UTIs), with recurrent cases often linked to its ability to form biofilms. This study investigated the effects of various antibiotics on UPEC biofilm formation and the subsequent interaction of these biofilms/their supernatants with human neutrophils. We determined the minimum inhibitory concen
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Efficacy of parainfluenza virus 5 (PIV5)-vectored intranasal COVID-19 vaccine as a single dose primer and booster against SARS-CoV-2 variants.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Influenza
Immunization with COVID-19 vaccines has greatly reduced COVID-19-related deaths and hospitalizations from SARS-CoV-2 infection, but waning immunity and the emergence of variants capable of immune escape indicate the need for annual vaccine updates or development of different SARS-CoV-2 vaccine platforms. Parainfluenza virus 5 (PIV5)-vectored intranasal COVID-19 vaccine with the
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Nucleoside Analogue with Thymidine Nucleobase Inhibits <i>Leishmania infantum</i> and Depolarizes the Plasma Membrane Potential In Vitro.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Immunology
EVOS M5000 Imaging System, Thermo Fisher Scientific).
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Iris setosa Pall. ex Link Extract Reveals Amoebicidal Activity against Acanthamoeba castellanii and Acanthamoeba polyphaga with Low Toxicity to Human Corneal Cells.
Cell type: Amoeba | Technique: Fluorescence imaging | Disease/area: Immunology
Acanthamoeba keratitis (AK) is a sight-threatening and difficult-to-treat ocular infection. The significant side effects of current AK treatments highlight the urgent need to develop a safe and effective AK medication. In this study, the amoebicidal activity of Iris setosa Pall. ex Link extract (ISE) against Acanthamoeba was examined and its specific amoebicidal mechanism was e
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