EVOS M5000 in Immunology, Infection & Host-Pathogen Research
Curated literature review of independent peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar, PubMed, PMC and DOI where available. Plankton & Zoom does not host paywalled content.
The EVOS M5000 appears in immunology and microbiology studies tracking viral infection, T-cell biology, intestinal inflammation, bacterial biofilms, and commensal viruses. These papers were found through independent literature searches. These papers were identified by searching the open-access literature and verifying that the full text explicitly mentions the EVOS M5000 Imaging System as a microscope; they are not part of the manufacturer’s curated citation list.
Hyperglycemia and systemic inflammation differentially shape immune dysregulation, tissue destruction, and microbiota in experimental periodontitis and peri-implantitis in diabetic mice.
Cell type: Immune cells / oral fibroblasts / osteoclasts
Imaging technique: Fluorescence/histology imaging
Disease area: Diabetes / periodontitis / peri-implantitis
AIM: To investigate the impact of hyperglycemia and systemic inflammation on experimental periodontitis/peri-implantitis in diabetic mice, focusing on osteoimmunological dysregulation and oral microbial alteration. MATERIALS AND METHODS: After implant placement, diabetic db/db mice were treated with Liraglutide, Indome...
Front Immunol PMID 42421935 — PMC13341294
Google Scholar | PubMed | PMC | DOI
Exosome-enriched extracellular vesicles are associated with Getah virus RNA and transmission-related readouts in vitro.
Cell type: Mammalian cells / exosome-enriched extracellular vesicles
Imaging technique: EVOS M5000 for extracellular vesicle imaging
Disease area: Getah virus / alphavirus infection
BACKGROUND: Getah virus (GETV) is a mosquito-borne alphavirus of veterinary importance with potential zoonotic relevance. Extracellular vesicles (EVs) can facilitate intercellular transfer of viral components, but their involvement in GETV infection remains unclear. METHODS: Exosome-enriched EV fractions were isolated ...
Front Cell Infect Microbiol PMID 42376320 — PMC13311032
Google Scholar | PubMed | PMC | DOI
Microbiome-driven alterations in tryptophan metabolism contribute to behavioral comorbidities in the Muc2 knockout mouse model of chronic colitis.
Cell type: Intestinal epithelial cells / gut microbiota
Imaging technique: Histology / fluorescence imaging
Disease area: Colitis / IBD / neurobehavioral comorbidities
Globally, the incidence of inflammatory bowel disease (IBD) is projected to reach 0.5% of the population by 2030, with increasing recognition of neurobehavioral comorbidities, including anxiety, depression, and cognitive dysfunction. The mechanisms underlying these comorbidities remain unclear but may involve interacti...
Gut Microbes PMID 42343156 — PMC13313184
Google Scholar | PubMed | PMC | DOI
An inflammasome-driven differentiation program in intestinal stem cells protects against Salmonella infection.
Cell type: Intestinal stem cells (Lgr5+)
Imaging technique: Live-cell / fluorescence imaging
Disease area: Salmonella infection
Intestinal stem cells (ISCs) are essential for sustaining epithelial renewal and barrier integrity, yet their role in orchestrating defense against enteric pathogens remains unclear. Here we identify a stem cell-intrinsic immune mechanism whereby Lgr5+ ISCs detect intracellular Salmonella enterica and activate an infla...
Nat Immunol PMID 42120792 — PMC13310766
Google Scholar | PubMed | PMC | DOI
Coronavirus protein interaction mapping in bat and human cells reveals network rewiring governing immune evasion and zoonotic potential.
Cell type: Bat and human cells (Vero-E6, HEK293T)
Imaging technique: Fluorescence microscopy
Disease area: COVID-19 / coronavirus / zoonosis
Coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), can cause severe disease in humans, whereas reservoir hosts such as horseshoe bats remain asymptomatic. To investigate how host-specific protein-protein interactions (PPIs) influence infection, we generated comparative PPI maps for S...
Cell Host Microbe PMID 42134328 — PMC13286030
Google Scholar | PubMed | PMC | DOI
Anellovirus protein encoded by ORF2/3 functions as the viral replication initiation protein.
Cell type: Human cell lines (HEK293T)
Imaging technique: Fluorescence microscopy
Disease area: Anellovirus infection / commensal virology
Anelloviridae is a family of single-stranded DNA viruses that are thought to be nonpathogenic and commensal. Despite their ubiquitous presence in human populations, little is known about the anellovirus mechanism of replication in host cells. We identified the protein coded by ORF2/3 as necessary and sufficient to init...
Proc Natl Acad Sci U S A PMID 41433061 — PMC12772153
Google Scholar | PubMed | PMC | DOI
Thymic cortical epithelial Psmb11-encoded β5t in mouse and human.
Cell type: Thymic cortical epithelial cells (cTECs)
Imaging technique: Histology / fluorescence imaging
Disease area: T-cell development / immunodeficiency
Psmb11-encoded β5t is a proteolytic subunit of thymoproteasomes expressed in cortical thymic epithelial cells (cTECs). β5t-containing thymoproteasomes are essential for the thymic generation of CD8-lineage cytotoxic T cells. Here, we outline the current understanding of the mechanisms underlying thymoproteasome-depende...
Int Immunol PMID 42261593 — PMC13281738
Google Scholar | PubMed | PMC | DOI
MK2 Inhibition in CD4+ T Cells Protects Against IFNγ and IL-17A, Chronic Inflammation, and Fibrosis in Inflammatory Bowel Disease Models.
Cell type: CD4+ T cells
Imaging technique: Fluorescence / histology imaging
Disease area: Inflammatory bowel disease (IBD)
BACKGROUND: CD4+ T cells contribute to chronic inflammation and fibrosis in inflammatory bowel disease (IBD), but the cellular mechanisms remain elusive. We have found that the mitogen-activated protein kinase 2 (MK2) pathway plays a major role in inflammation and overall pathology in IBD. Thus, here, we examined the r...
Inflamm Bowel Dis PMID 39937137 — PMC12582355
Google Scholar | PubMed | PMC | DOI
Label-free microscopy enables high-throughput identification of genes controlling biofilm development.
Cell type: Bacterial biofilms
Imaging technique: Label-free microscopy / brightfield high-throughput imaging
Disease area: Biofilm / persistent infection
UNLABELLED: Biofilms are structured microbial communities that thrive on diverse surfaces in natural, industrial, and host environments. The biofilm lifestyle underpins microbial survival, shapes ecosystem function, and drives persistent infections; yet, for many microbes, the molecular determinants of biofilm developm...
mBio PMID 42126270 — PMC13251385
Google Scholar | PubMed | PMC | DOI