EVOS M5000 in Infectious Disease & Immunology Research — Wave 3 Part 2
Peer-reviewed studies using the EVOS M5000 Imaging System for bacteria, viruses, COVID-19, host-pathogen interactions and immune cells. Each entry was discovered independently of Thermo Fisher's curated EVOS M5000 citations and verified to mention the EVOS M5000 Imaging System as a microscope in the full text. Paper cards link to Google Scholar, PubMed, PMC and DOI when available.
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Pneumolysin contributes to dysfunction of nasal epithelial barrier for promotion of pneumococcal dissemination into brain tissue.
Cell type: epithelial | Technique: general | Disease/area: infectious disease | mSphere (2024)
(Abstract unavailable)
Google Scholar | PubMed | PMC | DOI
Coxsackievirus B3 Activates Macrophages Independently of CAR-Mediated Viral Entry.
Cell type: macrophage | Technique: general | Disease/area: infectious disease, inflammation | Viruses (2024)
Enteroviruses are a genus of small RNA viruses that are responsible for approximately one billion global infections annually. These infections range in severity from the common cold and flu-like symptoms to more severe diseases, such as viral myocarditis, panc
Google Scholar | PubMed | PMC | DOI
The First Isolation of Insect-Specific Alphavirus (
Cell type: general | Technique: general | Disease/area: infectious disease | Viruses (2024)
Advances in diagnostic techniques coupled with ongoing environmental changes have resulted in intensified surveillance and monitoring of arbovirus circulation in the Amazon. This increased effort has resulted in increased detection of insect-specific viruses a
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Development of a Lipid-encapsulated TGFβRI-siRNA Drug for Liver Fibrosis Induced by Schistosoma mansoni.
Cell type: T cell | Technique: EdU proliferation | Disease/area: fibrosis, infectious disease | PLoS neglected tropical diseases (2024)
Schistosoma mansoni infection leads to chronic schistosomiasis and severe hepatic fibrosis. We designed a liver-targeted lipid nanoparticle (LNP) carrying siRNA against type I TGF-β receptor (TGFβRI) mRNA to treat schistosomiasis-induced liver fibrosis in BALB
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HIV-induced RSAD2/Viperin supports sustained infection of monocyte-derived macrophages.
Cell type: macrophage | Technique: general | Disease/area: infectious disease | Journal of virology (2024)
HIV establishes long-term latent infection in memory CD4 HIV infection of macrophages is a barrier to HIV cure and a source of neurocognitive pathology. We found that HIV induces RSAD2/Viperin during sustained infection of macrophages. While RSAD2/Viperin is a
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Synergistic nanocoating with layer-by-layer functionalized PCL membranes enhanced by manuka honey and essential oils for advanced wound healing.
Cell type: T cell, fibroblast | Technique: RT-qPCR, qPCR, wound healing | Disease/area: infectious disease, wound healing | Scientific reports (2024)
Chronic wounds represent a significant global health concern, statistically impacting 1-2% of the population in developed countries throughout their lifetimes. These wounds cause considerable discomfort for patients and necessitate substantial expenditures of
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Cell-Int: a cell-cell interaction assay to identify native membrane protein interactions.
Cell type: HEK293 | Technique: general | Disease/area: infectious disease | Life science alliance (2024)
Intercellular protein-protein interactions (PPIs) have pivotal roles in biological functions and diseases. Membrane proteins are therefore a major class of drug targets. However, studying such intercellular PPIs is challenging because of the properties of memb
Google Scholar | PubMed | PMC | DOI
EXTL3 and NPC1 are mammalian host factors for Autographa californica multiple nucleopolyhedrovirus infection.
Cell type: HEK293 | Technique: CRISPR | Disease/area: infectious disease | Nature communications (2024)
Baculovirus is an obligate parasitic virus of the phylum Arthropoda. Baculovirus including Autographa californica multiple nucleopolyhedrovirus (AcMNPV) has been widely used in the laboratory and industrial preparation of proteins or protein complexes. Due to
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Incompatible packaging signals and impaired protein functions hinder reassortment of bat H17N10 or H18N11 segment 7 with human H1N1 influenza A viruses.
Cell type: HEK293 | Technique: general | Disease/area: infectious disease | Journal of virology (2024)
Novel bat H17N10 and H18N11 influenza A viruses (IAVs) are incapable of reassortment with conventional IAVs during co-infection. To date, the underlying mechanisms that inhibit bat and conventional IAV reassortment remain poorly understood. Herein, we used the
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High mobility group box 1 (HMGB1) is a potential disease biomarker in cell and mouse models of Duchenne muscular dystrophy.
Cell type: iPSC | Technique: general | Disease/area: infectious disease, inflammation | Biology open (2024)
Duchenne muscular dystrophy (DMD) is a progressive muscle wasting disorder affecting 1:3500 male births and is associated with myofiber degeneration, regeneration, and inflammation. Glucocorticoid treatments have been the standard of care due to immunomodulato
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Plant-induced bacterial gene silencing: a novel control method for bacterial wilt disease.
Cell type: general | Technique: general | Disease/area: infectious disease | Frontiers in plant science (2024)
(Abstract unavailable)
Google Scholar | PubMed | PMC | DOI
Development and validation of Mayaro virus with luciferase reporter genes as a tool for antiviral assays.
Cell type: general | Technique: general | Disease/area: infectious disease | Heliyon (2024)
Arboviruses are etiological agents in an extensive group of emerging diseases with great clinical relevance in Brazil, due to the wide distribution of their vectors and the favorable environmental conditions. Among them, the Mayaro virus (MAYV) has drawn atten
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The role of FraI in cell-cell communication and differentiation in the hormogonia-forming cyanobacterium
Cell type: general | Technique: general | Disease/area: infectious disease | mSphere (2024)
Multicellular cyanobacteria, like The filament-forming cyanobacterium
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SARS-CoV-2 Spike Protein 1 Causes Aggregation of α-Synuclein via Microglia-Induced Inflammation and Production of Mitochondrial ROS: Potential Therapeutic Applications of Metformin.
Cell type: microglia, neuron | Technique: general | Disease/area: COVID-19, infectious disease, inflammation, neurodegenerative | Biomedicines (2024)
Abnormal aggregation of α-synuclein is the hallmark of neurodegenerative diseases, classified as α-synucleinopathies, primarily occurring sporadically. Their onset is associated with an interaction between genetic susceptibility and environmental factors such
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Neutralizing antibodies reveal cryptic vulnerabilities and interdomain crosstalk in the porcine deltacoronavirus spike protein.
Cell type: HEK293 | Technique: general | Disease/area: infectious disease | Nature communications (2024)
Porcine deltacoronavirus (PDCoV) is an emerging enteric pathogen that has recently been detected in humans. Despite this zoonotic concern, the antigenic structure of PDCoV remains unknown. The virus relies on its spike (S) protein for cell entry, making it a p
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Mycoplasma glycine cleavage system key subunit GcvH is an apoptosis inhibitor targeting host endoplasmic reticulum.
Cell type: T cell | Technique: EdU proliferation | Disease/area: infectious disease | PLoS pathogens (2024)
Mycoplasmas are minimal but notorious bacteria that infect humans and animals. These genome-reduced organisms have evolved strategies to overcome host apoptotic defense and establish persistent infection. Here, using Mycoplasma bovis as a model, we demonstrate
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Protecting the piglet gut microbiota against ETEC-mediated post-weaning diarrhoea using specific binding proteins.
Cell type: general | Technique: general | Disease/area: infectious disease | NPJ biofilms and microbiomes (2024)
Post-weaning diarrhoea (PWD) in piglets presents a widespread problem in industrial pig production and is often caused by enterotoxigenic E. coli (ETEC) strains. Current solutions, such as antibiotics and medicinal zinc oxide, are unsustainable and are increas
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Tetracyclic homoisoflavanoid (+)-brazilin: a natural product inhibits c-di-AMP-producing enzyme and
Cell type: general | Technique: general | Disease/area: infectious disease | Microbiology spectrum (2024)
The tenacious biofilms formed by
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An LIR motif in the Rift Valley fever virus NSs protein is critical for the interaction with LC3 family members and inhibition of autophagy.
Cell type: general | Technique: general | Disease/area: infectious disease | PLoS pathogens (2024)
Rift Valley fever virus (RVFV) is a viral zoonosis that causes severe disease in ruminants and humans. The nonstructural small (NSs) protein is the primary virulence factor of RVFV that suppresses the host's antiviral innate immune response. Bioinformatic
Google Scholar | PubMed | PMC | DOI
Host and nonhost bacteria support bacteriophage dissemination along mycelia and abiotic dispersal networks.
Cell type: general | Technique: general | Disease/area: infectious disease | microLife (2024)
Bacteriophages play a crucial role in shaping bacterial communities, yet the mechanisms by which nonmotile bacteriophages interact with their hosts remain poorly understood. This knowledge gap is especially pronounced in structured environments like soil, wher
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Particulate Matter Induces Oxidative Stress and Ferroptosis in Human Lung Epithelial Cells.
Cell type: T cell, endothelial, epithelial | Technique: RT-qPCR, qPCR | Disease/area: infectious disease | Toxics (2024)
Numerous toxicological studies have highlighted the association between urban particulate matter (PM) and increased respiratory infections and lung diseases. The adverse impact on the lungs is directly linked to the complex composition of particulate matter, i
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Activation of protein kinase receptor (PKR) plays a pro-viral role in mammarenavirus-infected cells.
Cell type: T cell, cell line | Technique: EdU proliferation | Disease/area: infectious disease | Journal of virology (2024)
Many viruses, including mammarenaviruses, have evolved mechanisms to counteract different components of the host cell innate immunity, which is required to facilitate robust virus multiplication. The double-stranded RNA (dsRNA) sensor protein kinase receptor (
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All-in-one IQ toggle switches with high versatilities for fine-tuning of transgene expression in mammalian cells and tissues.
Cell type: cell line | Technique: transfection | Disease/area: infectious disease | Molecular therapy. Methods & clinical development (2024)
The transgene toggling device is recognized as a powerful tool for gene- and cell-based biological research and precision medicine. However, many of these devices often operate in binary mode, exhibit unacceptable leakiness, suffer from transgene silencing, sh
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Rapid conversion of porcine pluripotent stem cells into macrophages with chemically defined conditions.
Cell type: macrophage | Technique: general | Disease/area: infectious disease | The Journal of biological chemistry (2024)
A renewable source of porcine macrophages derived from pluripotent stem cells (PSCs) would be a valuable alternative to primary porcine alveolar macrophages (PAMs) in the research of host-pathogen interaction mechanisms. We developed an efficient and rapid pro
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Cholesterol 25-Hydroxylase Suppresses Swine Acute Diarrhea Syndrome Coronavirus Infection by Blocking Spike Protein-Mediated Membrane Fusion.
Cell type: epithelial | Technique: general | Disease/area: infectious disease | Viruses (2023)
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is an emerging porcine intestinal coronavirus that can cause acute diarrhea, vomiting, rapid weight loss, and high mortality in newborn piglets. Cholesterol 25-hydroxylase (CH25H) is a molecular mediator of
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Embedded macrophages induce intravascular coagulation in 3D blood vessel-on-chip.
Cell type: endothelial, iPSC, macrophage | Technique: wound healing | Disease/area: atherosclerosis, infectious disease, inflammation, wound healing | Biomedical microdevices (2023)
Macrophages are innate immune cells that prevent infections and help in wound healing and vascular inflammation. While these cells are natural helper cells, they also contribute to chronic diseases, e.g., by infiltrating the endothelial layer in early atherosc
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Identification of a morphogene required for tapered filament termini in filamentous cyanobacteria.
Cell type: general | Technique: general | Disease/area: infectious disease | Microbiology (Reading, England) (2023)
Although the photosynthetic cyanobacteria are monophyletic, they exhibit substantial morphological diversity across species, and even within an individual species due to phenotypic plasticity in response to life cycles and environmental signals. This is partic
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Persistent paramyxovirus infections: in co-infections the parainfluenza virus type 5 persistent phenotype is dominant over the lytic phenotype.
Cell type: general | Technique: GFP | Disease/area: infectious disease | The Journal of general virology (2023)
Parainfluenza virus type 5 (PIV5) can either have a persistent or a lytic phenotype in cultured cells. We have previously shown that the phenotype is determined by the phosphorylation status of the phosphoprotein (P). Single amino acid substitutions at critica
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Plant flavonoid inhibition of SARS-CoV-2 main protease and viral replication.
Cell type: general | Technique: general | Disease/area: COVID-19, infectious disease | iScience (2023)
Plant-based flavonoids have been evaluated as inhibitors of β-coronavirus replication and as therapies for COVID-19 on the basis of their safety profile and widespread availability. The SARS-CoV-2 main protease (Mpro) has been implicated as a target for flavon
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Generation of a recombinant Saffold Virus expressing UnaG as a marker for the visualization of viral infection.
Cell type: HeLa | Technique: GFP | Disease/area: infectious disease | Virology journal (2023)
Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and asepti
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Establishment of a CPER reverse genetics system for Powassan virus defines attenuating NS1 glycosylation sites and an infectious NS1-GFP11 reporter virus.
Cell type: general | Technique: EdU proliferation, GFP | Disease/area: infectious disease, skin disease | mBio (2023)
Powassan virus (POWV) is an emerging tick-borne Flavivirus that causes lethal encephalitis and long-term neurologic damage. Currently, there are no POWV therapeutics, licensed vaccines, or reverse genetics systems for producing infectious POWVs from recombinan
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Effects of pH alteration on respiratory syncytial virus in human airway epithelial cells.
Cell type: epithelial | Technique: GFP, western blot | Disease/area: infectious disease, inflammation | ERJ open research (2023)
Respiratory syncytial virus (RSV) is a leading cause of respiratory distress and hospitalisation in the paediatric population. Low airway surface pH impairs antimicrobial host defence and worsens airway inflammation. Inhaled Optate safely raises airway surface
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Luteolin Isolated from
Cell type: general | Technique: general | Disease/area: COVID-19, infectious disease | Molecules (Basel, Switzerland) (2023)
The COVID-19 pandemic, caused by SARS-CoV-2, addressed the lack of specific antiviral drugs against coronaviruses. In this study, bioguided fractionation performed on both ethyl acetate and aqueous sub-extracts of
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Cationic Microbubbles for Non-Selective Binding of Cavitation Nuclei to Bacterial Biofilms.
Cell type: general | Technique: general | Disease/area: infectious disease | Pharmaceutics (2023)
The presence of multi-drug resistant biofilms in chronic, persistent infections is a major barrier to successful clinical outcomes of therapy. The production of an extracellular matrix is a characteristic of the biofilm phenotype, intrinsically linked to antim
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Optogenetic activation of UCP1-dependent thermogenesis in brown adipocytes.
Cell type: adipocyte | Technique: general | Disease/area: infectious disease, obesity | iScience (2023)
Brown adipocytes are unique in that they expend energy and produce heat to maintain euthermia through expression of uncoupling protein-1 (UCP1). Given their propensity to stimulate weight loss and promote resistance to obesity, they are a compelling interventi
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Degradation of EGFR on lung epithelial cells by neutrophil elastase contributes to the aggravation of pneumococcal pneumonia.
Cell type: T cell, epithelial | Technique: EdU proliferation | Disease/area: infectious disease | The Journal of biological chemistry (2023)
Pneumococcus is the main cause of bacterial pneumonia. Pneumococcal infection has been shown to cause elastase, an intracellular host defense factor, to leak from neutrophils. However, when neutrophil elastase (NE) leaks extracellularly, it can degrade host ce
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A Triboelectric Nanocomposite for Sterile Sensing, Energy Harvesting, and Haptic Diagnostics in Interventional Procedures from Surgical Gloves.
Cell type: general | Technique: EdU proliferation | Disease/area: infectious disease | Advanced healthcare materials (2023)
Advanced interfacial engineering has the potential to enable the successful realization of three features that are particularly important for a variety of healthcare applications: wettability control, antimicrobial activity to reduce infection risks, and sensi
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Epidemiological, Clinical, and Virological Investigation of the First Four Cases of Monkeypox in Cartagena during the 2022 Outbreak.
Cell type: general | Technique: general | Disease/area: infectious disease | Pathogens (Basel, Switzerland) (2023)
Since early May 2022, numerous cases of Monkeypox (Mpox) have been reported globally in non-endemic areas. However, despite numerous reports worldwide, the epidemiological and genomic information related to the 2022 multi-country outbreak remains scarce in Sou
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Cellular and Biochemical Characterization of Mesenchymal Stem Cells from Killian Nasal Polyp.
Cell type: MSC, adipocyte, epithelial, osteoblast | Technique: EdU proliferation | Disease/area: infectious disease, inflammation | International journal of molecular sciences (2022)
Killian's (antrochoanal) polyp is a unilateral nasal polypoid lesion of the maxillary sinus especially affecting children and young adults with unilateral nasal obstruction, pus discharge, and headache. Although its etiology is unclear, chronic inflammati
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Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge.
Cell type: general | Technique: general | Disease/area: infectious disease | Frontiers in microbiology (2022)
African swine fever virus (ASFV) represents a serious threat to the global swine industry, and there are no safe or commercially available vaccines. Previous studies have demonstrated that inactivated vaccines do not provide sufficient protection against ASFV
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Efficient elimination of airborne pathogens: a study on aerosolized Mycobacterium tuberculosis and SARS-CoV-2 using ZeBox technology.
Cell type: general | Technique: EdU proliferation | Disease/area: COVID-19 | The Journal of hospital infection (2022)
Despite multifactorial evidence, the safe and effective elimination of free-floating micro-organisms remains a significant scientific challenge. ZeBox Technology exploits microbial Zeta Potential, to extract and eliminate them from free-flowing air, using a no
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Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs.
Cell type: cell line | Technique: CRISPR, knockout | Disease/area: COVID-19, infectious disease | Nature genetics (2022)
CRISPR knockout (KO) screens have identified host factors regulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication. Here, we conducted a meta-analysis of these screens, which showed a high level of cell-type specificity of the identi
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A Fluorometric Assay for the
Cell type: general | Technique: general | Disease/area: infectious disease | Microbiology spectrum (2022)
Primary amoebic meningoencephalitis (PAM) is a lethal and rapid infection that affects the central nervous system and is caused by the free-living amoeba Naegleria fowleri. The life cycle of this protozoa consists of three different stages: The trophozoite, fl
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(Title unavailable)
Cell type: general | Technique: general | Disease/area: infectious disease | Frontiers in cellular and infection microbiology (2022)
(Abstract unavailable)
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Clofoctol inhibits SARS-CoV-2 replication and reduces lung pathology in mice.
Cell type: general | Technique: EdU proliferation | Disease/area: COVID-19, infectious disease, inflammation | PLoS pathogens (2022)
Drug repurposing has the advantage of shortening regulatory preclinical development steps. Here, we screened a library of drug compounds, already registered in one or several geographical areas, to identify those exhibiting antiviral activity against SARS-CoV-
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AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses.
Cell type: general | Technique: general | Disease/area: infectious disease | STAR protocols (2022)
The AdenoBuilder platform enables the
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N-acyl-homoserine lactone produced by Rahnella inusitata isolated from the gut of Galleria mellonella influences Salmonella phenotypes.
Cell type: general | Technique: general | Disease/area: infectious disease | Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2022)
The most studied mechanism of quorum sensing in Gram-negative bacteria is mediated by autoinducer 1 (AI-1), namely, acyl-homoserine lactone (AHL). This system allows communication among different bacterial species and regulates the expression of virulence gene
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Preparation of Giant
Cell type: general | Technique: general | Disease/area: infectious disease | Bio-protocol (2021)
Prokaryotic ion channels have been instrumental in furthering our understanding of many fundamental aspects of ion channels' structure and function. However, characterizing the biophysical properties of a prokaryotic ion channel in a native membrane syste
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Construction, Identification and Analysis of the Interaction Network of African Swine Fever Virus MGF360-9L with Host Proteins.
Cell type: macrophage | Technique: EdU proliferation | Disease/area: infectious disease | Viruses (2021)
African swine fever virus (ASFV) is prevalent in many countries and is a contagious and lethal virus that infects pigs, posing a threat to the global pig industry and public health. The interaction between the virus and the host is key to unlocking the mystery
Google Scholar | PubMed | PMC | DOI
SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway.
Cell type: cardiomyocyte, cell line | Technique: general | Disease/area: COVID-19, infectious disease | Journal of virology (2021)
SARS-CoV-2 can infect multiple organs, including lung, intestine, kidney, heart, liver, and brain. The molecular details of how the virus navigates through diverse cellular environments and establishes replication are poorly defined. Here, we generated a panel
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Amino Acid Residues Involved in Inhibition of Host Gene Expression by Influenza A/Brevig Mission/1/1918 PA-X.
Cell type: general | Technique: general | Disease/area: infectious disease, inflammation | Microorganisms (2021)
The influenza A virus (IAV) PA-X protein is a virulence factor that selectively degrades host mRNAs leading to protein shutoff. This function modulates host inflammation, antiviral responses, cell apoptosis, and pathogenesis. In this work we describe a novel a
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The in-vitro effect of famotidine on sars-cov-2 proteases and virus replication.
Cell type: A549 | Technique: EdU proliferation | Disease/area: COVID-19, infectious disease | Scientific reports (2021)
The lack of coronavirus-specific antiviral drugs has instigated multiple drug repurposing studies to redirect previously approved medicines for the treatment of SARS-CoV-2, the coronavirus behind the ongoing COVID-19 pandemic. A recent, large-scale, retrospect
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Engineering SARS-CoV-2 using a reverse genetic system.
Cell type: general | Technique: general | Disease/area: COVID-19, infectious disease | Nature protocols (2021)
Reverse genetic systems are a critical tool for studying viruses and identifying countermeasures. In response to the ongoing COVID-19 pandemic, we recently developed an infectious complementary DNA (cDNA) clone for severe acute respiratory syndrome coronavirus
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Ultrastructural modifications induced by SARS-CoV-2 in Vero cells: a kinetic analysis of viral factory formation, viral particle morphogenesis and virion release.
Cell type: T cell, cell line | Technique: general | Disease/area: COVID-19, infectious disease | Cellular and molecular life sciences : CMLS (2021)
Many studies on SARS-CoV-2 have been performed over short-time scale, but few have focused on the ultrastructural characteristics of infected cells. We used TEM to perform kinetic analysis of the ultrastructure of SARS-CoV-2-infected cells. Early infection eve
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Generation and Characterization of recombinant SARS-CoV-2 expressing reporter genes.
Cell type: general | Technique: general | Disease/area: COVID-19, infectious disease | Journal of virology (2021)
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the pathogen responsible of coronavirus disease 2019 (COVID-19), has devastated public health services and economies worldwide. Despite global efforts to contain the COVID-19 pandem
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Multiple Lines of Evidences Reveal Mechanisms Underpinning Mercury Resistance and Volatilization by Stenotrophomonas sp. MA5 Isolated from the Savannah River Site (SRS), USA.
Cell type: general | Technique: general | Disease/area: infectious disease | Cells (2019)
A largely understudied microbially mediated mercury (Hg) bioremediative pathway includes the volatilization of Hg
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