EVOS M5000 in Neuroscience & Neurodegeneration: Independent Studies — Wave 2
Brain, neuron and neurodegeneration research with the EVOS M5000 microscope. Each paper was discovered independently of Thermo Fisher's curated EVOS M5000 citations and verified to mention the EVOS M5000 Imaging System as a microscope in the full text. Paper cards link to Google Scholar, PubMed, PMC and DOI.
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Ginsenosides Rb3 and Rc Exhibit Anti-Amoebic Activities Against Naegleria fowleri, the Etiological Agent of Primary Amoebic Meningoencephalitis.
Cell type: Amoeba | Technique: Fluorescence imaging | Disease/area: Neuroscience
Background/Objectives:Naegleria fowleri is an opportunistic pathogen causing primary amoebic meningoencephalitis (PAM), a fatal neuroinflammatory disease with a high mortality rate of over 97%, in humans. Currently, there are no approved therapeutics for PAM, underscoring the urgent necessity of developing effective and safe drugs. This study aimed to evaluate the potential of
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Aloin attenuates NMDA receptor by modulating calcium response on substantia gelatinosa neurons of the trigeminal subnucleus caudalis in mice.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience
Aloe vera extracts, particularly aloin, have been widely used in traditional Chinese medicine for their anti-inflammatory and detoxifying properties. Recent studies suggest that aloin may also exert neuroprotective effects, though its specific mechanisms remain unclear. Glutamate excitotoxicity, a critical factor in neurodegenerative and pain-related disorders, is mediated prim
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Antler stem cell-derived exosomes restore periodontal homeostasis in a rat model with diabetic periodontitis through enhancing ROS scavenging and osteogenesis.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Neuroscience
Diabetes mellitus (DM) exacerbates periodontitis as the high-glucose (HG) environment aggravates local inflammation and periodontal bone resorption. Restoring periodontal homeostasis and promoting periodontal bone repair/regeneration are major challenges for the treatment of diabetic periodontitis. This study introduces antler stem cell-derived exosomes (AnSC-exos) as a potent
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Flucofuron as a Promising Therapeutic Agent against Brain-Eating Amoeba.
Cell type: Amoeba | Technique: Fluorescence imaging | Disease/area: Neuroscience
Primary amoebic meningoencephalitis (PAM) is a rare and fulminant neurodegenerative disease caused by the free-living amoeba Naegleria fowleri. Currently, there is a lack of standardized protocols for therapeutic action. In response to the critical need for effective therapeutic agents, we explored the Global Health Priority Box, a collection of 240 compounds provided by the Me
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Protocol for 3D Matrigel embedding of primary murine astrocytes for a physiologically relevant culture model.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Neuroscience
Primary astrocyte 2D cultures often exhibit a phenotype that differs significantly from astrocytes in the brain. Here, we present a detailed protocol for culturing primary murine astrocytes within a 3D Matrigel environment, which better mimics the in vivo conditions. We describe a step-by-step guide for the isolation and preparation of primary striatal astrocytes, their embeddi
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Fucoxanthin Inhibits the NMDA and AMPA Receptors Through Regulating the Calcium Response on Substantia Gelatinosa Neurons of the Trigeminal Subnucleus Caudalis in Juvenile Mice.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience
EVOS M5000, Thermo Fisher, MA, USA). To visualize the variation in the spontaneous activity of Ca<sup>2+</sup> signals in the application of FCX, the laminae I–II regions over the field of view were selected, a
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Identification and characterization of novel marine oxasqualenoid yucatecone against Naegleria fowleri.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Neuroscience
EVOS M5000 Cell Imaging System (Life Technologies, Madrid, Spain) was used and each experiment was performed in triplicate. In each experiment five different images were evaluated with a minimum number of cells
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N-butylphthalide (NBP) and ligustrazine (TMP) triazole hybrids target the KEAP1-NRF2 pathway to inhibit ferroptosis and exert brain neuroprotectivity.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience
Ischemic stroke is a life-threatening disease, its pathological progression involves multiple factors, including oxidative stress, apoptosis, and ferroptosis. Our previous study demonstrated that hybridizing N-butylphthalide (NBP) with ligustrazine (TMP) yielded promising anti-ischemic compounds. In this study, we further introduced a triazole structure into NBP-TMP hybrids and
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A partial deletion of the Tardbp 3'UTR affects TDP-43 regulation and leads to motor dysfunction in mice.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease that causes the selective loss of motor neurons. A histopathological hallmark of ALS is the cytoplasmic aggregation of TDP-43, a ubiquitously expressed RNA-binding protein involved in transcription and splicing regulation. To prevent abnormal accumulation, TDP-43 controls its expression levels throug
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Repurposing of Nitroxoline as an Alternative Primary Amoebic Meningoencephalitis Treatment.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Neuroscience
Among the pathogenic free-living amoebae (FLA), Naegleria fowleri is the etiological agent of a fatal disease known as primary amoebic meningoencephalitis (PAM). Once infection begins, the lesions generated in the central nervous system (CNS) result in the onset of symptoms leading to death in a short period of time. Currently, there is no standardized treatment against the inf
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Lack of Oxygen and/or Glucose Differentially Potentiates Aβ40e22q- and Aβ42-Induced Cerebral Endothelial Cell Death, Barrier Dysfunction and Angiogenesis Impairment.
Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Alzheimer
Cerebrovascular damage/dysfunction promote cerebral hypoperfusion early within Alzheimer's Disease (AD). Cerebral hypoperfusion is also a common consequence of cardiovascular risk factors/diseases, typically manifesting in midlife when AD pathology initiates, and contributing to AD onset/progression. We demonstrated that AβQ22 (vasculotropic Dutch mutant) and Aβ42 promote cereb
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Resveratrol Analogs Ameliorate Mitochondrial Impairment and Insulin Resistance in a Streptozotocin-Induced In Vitro Model of Alzheimer's Disease.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Alzheimer
Alzheimer's disease (AD) is characterized by mitochondrial dysfunction, oxidative stress, insulin resistance, and aberrant protein aggregation. Neurodegeneration model with neuronal insulin resistance was induced in SH-SY5Y human neuroblastoma cells by streptozotocin (STZ). We evaluated the neuroprotective effects of resveratrol (RZV) and three structural analogs: oxyresveratro
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