EVOS M5000 in Neuroscience & Neurodegeneration: Independent Studies — Wave 3 Part 2
Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.
EVOS M5000IndependentWave 3
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Protocol for modeling injury-induced regeneration in retinal organoids and isolating hNRSCs for subretinal transplantation in rd10 mice.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Human retinal organoids (hROs) provide a platform for modeling injury and assessing regenerative capacity in vitro. Here, we present a protocol for stepwise generation of hROs from human embryonic stem cells (hESCs). We describe steps for microsurgical induction of neural retina (NR) injury, isolation of CPAMD8
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MeCP2 regulates telencephalic development in human cerebral organoids.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Loss-of-function variants in the X-linked gene MeCP2 cause a severe form of syndromic autism spectrum disorder known as Rett syndrome (RTT). Although traditionally considered a postnatal disorder, increasing evidence suggests that MeCP2 plays a crucial role during prenatal brain development. Here, we used human pluripotent stem cell-derived cerebral organoids and human telencep
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The Glycosaminoglycan-Dependent Interactome of Neurexin-1 in Human Fetal Glial Cells.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Neurexins are synaptic adhesion molecules best characterized in neurons, where they regulate synapse assembly and function, with emerging evidence indicating they are also abundantly expressed by astrocytes. To elucidate the interactome of NRXN1α, we employed a proximity labeling strategy in cultured human fetal glial cells (SVG p12 cells). This approach enables the identificat
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Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
RNA-binding proteins (RBPs) with prion-like domains (PrLDs), such as FUS and TDP-43, condense into functional liquids, which can transform into pathological fibrils that underpin fatal neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS)/frontotemporal dementia (FTD). Here, we define short RNAs that prevent FUS fibrillization by promoting liquid phases and
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Stress resilience is associated with transcriptional remodeling in the VTA.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Individual responses to chronic stress vary, with some individuals remaining resilient while others exhibit susceptibility. Here, we use single-nucleus RNA sequencing to understand molecular adaptations in the ventral tegmental area (VTA) and lateral habenula (LHb) that mediate susceptibility vs. resilience to social stress. While we find minimal gene expression changes in the
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Autophagy Activators Normalize Aberrant Tau Proteostasis and Rescue Synapses in Human Familial Alzheimer's Disease iPSC-Derived Cortical Organoids.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Alzheimer's disease (AD) is the leading cause of dementia worldwide. Nevertheless, its cellular and molecular mechanisms remain incompletely understood, partially due to inadequate disease models. To illuminate early changes in AD, we developed a cerebrocortical organoid (CO) model with improved methodology. Our COs produce excitatory and inhibitory neurons alongside glia, util
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Microvascular pathology in the spinal cord of severe spinal muscular atrophy patients.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Severe spinal muscular atrophy (SMA) is a life-limiting neurodegenerative disease of infancy and early childhood, caused by reduced expression of the ubiquitous survival motor neuron protein (SMN). While current therapies aim to increase SMN levels and preserve motor neurons, significant deficits remain in treated patients and non-neuronal manifestations of SMN deficiency are u
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Guided and unguided neural organoids play complementary roles in studying neurodevelopment and neuroinflammation.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
This study aimed to compare guided dorsal forebrain neural organoids with unguided neural organoids, focusing on differences in structural organization, cellular composition, and functional properties. Using the same human induced pluripotent stem cell line, we applied two established differentiation protocols in parallel to generate guided and unguided neural organoids. Guided
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Remote Ischemic Preconditioning Exerts Neuroprotective Effects Via the PGC-1α/FNDC5/BDNF Pathway in Focal Brain Ischemia of Rats.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Remote ischemic preconditioning (RIpreC) is a strategy for remotely protecting target organs such as the brain by applying brief ischemia and reperfusion to the limb. However, the mechanisms underlying RIpreC-induced neuroprotection remain unclear. We aimed to investigate the neuroprotective effects of RIpreC on the peroxisome proliferator-activated receptor-gamma coactivator 1
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Microglia cause HIV-induced transcriptional and metabolic changes in human neural organoids.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Human immunodeficiency virus (HIV) can invade the central nervous system during the initial stages of infection and contribute to HIV-associated neurocognitive disorder, affecting up to 50% of people living with HIV (PLWH). To investigate HIV-1-induced immunometabolic changes in the brain, we used a three-dimensional microglia-embedded human neural organoid model. Transcriptomi
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GABA-Induced Exosomes Improve Memory Impairment in Aged Mice.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Gamma-aminobutyric acid (GABA) has been implicated in gut-brain interactions and neuronal activation. We hypothesized that GABA could ameliorate memory decline. We investigated whether oral GABA administration ameliorated age-related cognitive decline in aged mice (C57BL/6J, male) and explored the role of circulating exosomes in mediating these effects. Aged mice that drank wat
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Morphological Features and HIF1-Dependent Processes in the Brain of Progeny of Female Rats Exposed to Maternal Hypoxia.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Fetal hypoxia and maternal stress during pregnancy are major risk factors for neurological disorders. The effects of maternal hypoxia may be transmitted to the next generation through persistent alterations in maternal endocrine and metabolic regulation. In this study, using immunohistochemistry, quantitative RT-PCR, and Western blotting, we assessed morphological features and
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The Antidepressant Amitriptyline Upregulates ERK1/2 Signaling and Inhibits Rho-Mediated Responses Induced by Lysophosphatidic Acid in Astroglial Cells.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
(1) Different classes of antidepressant drugs have been shown to activate lysophosphatidic acid (LPA) receptors, but their effects on the receptor signaling stimulated by LPA have not been fully investigated. In the present study, we examined the effect of the tricyclic antidepressant amitriptyline on the LPA-induced activation of extracellular signal-regulated kinases 1 and 2
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APOE3-Christchurch variant enhances neurovascular support functions of iPSC-derived mesenchymal stromal cells.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Aging and neurodegenerative disorders like Alzheimer's Disease (AD) are associated with progressive dysfunction of the blood-brain barrier (BBB) and neurovascular unit (NVU), contributing to impaired vascular integrity and neuronal vulnerability. Apolipoprotein E (APOE) is a key regulator of neurovascular function, and the rare
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Isoform-specific steric zippers drive aberrant assembly and mislocalization of shortened TDP-43.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Neuroscience & Neurodegeneration
Prion-like domain (PrLD)-mediated aggregation and concomitant dysfunction of the essential RNA-binding protein transactive response (TAR) DNA-binding protein of 43 kilodaltons (TDP-43) is a common feature of multiple debilitating neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). However, shortened TDP-43 (sTDP-43) splice isoforms where the PrLD is larg
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