EVOS M5000 in Skin, Wound & Biomaterials: Independent Studies — Wave 3

Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.

EVOS M5000IndependentWave 3

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Effects of

Cell type: Keratinocyte | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Prodigiosins, which are natural tripyrrole red pigments and synthetic derivatives, reportedly have multiple biological effects mainly on various types of cancer cells. However, the effects of bacterial prodigiosin on non-cancerous HaCaT human skin keratinocytes have not been reported. Therefore, the present study aimed to investigate the functional activities of prodigiosin der

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Human Amniotic Fluid Stem Cell-Derived Exosomes as a Novel Cell-Free Therapy for Cutaneous Regeneration.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Adult wound healing often results in fibrotic scarring that is caused by myofibroblast aggregation. Human amniotic fluid stem cells (hAFSCs) exhibit significantly anti-fibrotic scarring properties during wound healing. However, it is little known whether hAFSCs directly or indirectly (paracrine) contribute to this process. Using the full-thickness skin-wounded rats, we investig

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Human amniotic epithelial cell transplantation improves scar remodeling in a rabbit model of acute vocal fold injury: a pilot study.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

To gain insight into the molecular mechanisms underlying the early stages of vocal fold extracellular matrix (ECM) remodeling after a mid-membranous injury resulting from the use of human amniotic epithelial cells (hAEC), as a novel regenerative medicine cell-based therapy. Vocal folds of six female, New Zealand White rabbits were bilaterally injured. Three rabbits had immediat

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Controlled Release of Epidermal Growth Factor from Furfuryl-Gelatin Hydrogel Using in Situ Visible Light-Induced Crosslinking and Its Effects on Fibroblasts Proliferation and Migration.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Hydrogels are widely used in tissue engineering as materials that regulate cell proliferation, migration, and differentiation. They also act as promising biomaterials that can provide a variety of stimuli by influencing the surrounding microenvironment, which can be achieved by modulating their mechanical properties, thereby aiding soluble factor delivery. Here, we developed a

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Fucosterol Isolated from Dietary Brown Alga

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

<i>Sargassum horneri</i> is a well-known edible brown alga that is widely abundant in the sea near China, Korea, and Japan and has a wide range of bioactive compounds. Fucosterol (FST), which is a renowned secondary metabolite in brown algae, was extracted from <i>S. horneri</i> to 70% ethanol, isolated via high-performance liquid chromatography (HPLC), followed by the immiscib

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Targeting SDF-1 as an efficient strategy to resolve skin hyperpigmentation issues with Himanthalia elongata extract.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

During aging, human skin is facing hyperpigmentation disorders: senile lentigo (chronobiologic aging) leads to loss of melanogenesis' control while solar lentigo (UV exposure) promotes an increase of oxidized proteins, melanogenesis, and lipofuscin. Stromal-cell-derived-factor-1 (SDF-1) was identified as key regulator of hyperpigmentation and its expression is reduced in senesc

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<i>PEX6</i> Mutations in Peroxisomal Biogenesis Disorders: An Usher Syndrome Mimic.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Peroxisomal biogenesis disorders (PBDs) represent a spectrum of conditions that result in vision loss, sensorineural hearing loss, neurologic dysfunction, and other abnormalities resulting from aberrant peroxisomal function caused by mutations in Laboratory-based study. A 12-year-old boy sought treatment with hearing loss and retinopathy. After negative results in an Usher synd

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Electrospinning vs. Electro-Assisted Solution Blow Spinning for Fabrication of Fibrous Scaffolds for Tissue Engineering.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Biodegradable polymeric fibrous non-woven materials are widely used type of scaffolds for tissue engineering. Their morphology and properties could be controlled by composition and fabrication technology. This work is aimed at development of fibrous scaffolds from a multicomponent polymeric system containing biodegradable synthetic (polylactide, polycaprolactone) and natural (g

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Supramolecular hybrid hydrogels as rapidly on-demand dissoluble, self-healing, and biocompatible burn dressings.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Despite decades of efforts, state-of-the-art synthetic burn dressings to treat partial-thickness burns are still far from ideal. Current dressings adhere to the wound and necessitate debridement. This work describes the first "supramolecular hybrid hydrogel (SHH)" burn dressing that is biocompatible, self-healable, and on-demand dissoluble for easy and trauma-free removal, prep

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Wound Dressings Based on Sodium Alginate-Polyvinyl Alcohol-

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Biopolymers have significant pharmaceutical applications, and their blending has favorable characteristics for their pharmaceutical properties compared to the sole components. In this work, sodium alginate (SA) as a marine biopolymer was blended with poly(vinyl) alcohol (PVA) to form SA/PVA scaffolds through the freeze-thawing technique. Additionally, polyphenolic compounds in

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Antler stem cell exosomes alleviate pulmonary fibrosis via inhibiting recruitment of monocyte macrophage, rather than polarization of M2 macrophages in mice.

Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Pulmonary fibrosis (PF), a chronic interstitial lung disease, is characterized by over-abundant deposition of extracellular matrix consisting mainly of collagen I. In previous studies, we demonstrated that deer antler stem cells (AnSCs), a novel type of adult stem cell, are capable of significantly down-regulating collagen formation in different organs and tissues and speculate

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Inflammation-mediated fibroblast activation and immune dysregulation in collagen VII-deficient skin.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Inflammation is known to play a critical role in all stages of tumorigenesis; however, less is known about how it predisposes the tissue microenvironment preceding tumor formation. Recessive dystrophic epidermolysis bullosa (RDEB), a skin-blistering disease secondary to

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Tocotrienol-Rich Fraction Attenuates Blue Light-Induced Oxidative Stress and Melanogenesis in B16-F1 Melanocytes via Anti-Oxidative and Anti-Tyrosinase Properties.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Our skin is constantly exposed to blue light (BL), which is abundant in sunlight and emitted by digital devices. Prolonged exposure to BL can lead to oxidative stress-induced damages and skin hyperpigmentation. For this study, we used a cell line-based model to examine the protective effects of tocotrienol-rich fraction (TRF) on BL-induced oxidative stress and hyperpigmentation

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Lipid Nanoparticle-Mediated Hit-and-Run Approaches Yield Efficient and Safe

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Despite exciting advances in gene editing, the efficient delivery of genetic tools to extrahepatic tissues remains challenging. This holds particularly true for the skin, which poses a highly restrictive delivery barrier. In this study, we ran a head-to-head comparison between Cas9 mRNA or ribonucleoprotein (RNP)-loaded lipid nanoparticles (LNPs) to deliver gene editing tools i

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Retention of Key Characteristics of Unprocessed Chorion Tissue Resulting in a Robust Scaffold to Support Wound Healing.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Placental membranes have been widely studied and used clinically for wound care applications, but there is limited published information on the benefits of using the chorion membrane. The chorion membrane represents a promising source of placental-derived tissue to support wound healing, with its native composition of extracellular matrix (ECM) proteins and key regulatory prote

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Investigating the Impact of Mechanical Properties and Cell-Collagen Interaction on NIH3T3 Function: A Comparative Study on Different Substrates and Culture Environments.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

This study investigates the intricate dynamics of matrix stiffness, substrate composition, and cell-cell interactions and elucidates their cumulative effects on fibroblast behavior in different culture contexts. Three primary substrate types were examined: non-coated, collagen-coated, and collagen hydrogel, within both two-dimensional (2D) monolayer and three-dimensional (3D) s

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Mechanical properties, and

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

A novel biomimetic dual layered keratin/hydroxyapatite (keratin/HA) scaffold was designed using iterative freeze-drying technique. The prepared scaffolds were studied using several analytical techniques to better understand the biological, structural, and mechanical properties. The developed multilayered, interconnected, porous keratin scaffold with different hydroxyapatite (HA

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Alternative mRNA splicing events and regulators in epidermal differentiation.

Cell type: Keratinocyte | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Alternative splicing (AS) of messenger RNAs occurs in ∼95% of multi-exon human genes and generates diverse RNA and protein isoforms. We investigated AS events associated with human epidermal differentiation, a process crucial for skin function. We identified 6,413 AS events, primarily involving cassette exons. We also predicted 34 RNA-binding proteins (RBPs) regulating epiderma

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DCM-Spheroid Morphs Express PADs and Citrullinated Cytoskeletal Proteins.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

During investigating the role of peptidylarginine deiminase (PAD) enzymes in dilated cardiomyopathy (DCM), we observed unique spheroid formation in DCM-myofibroblasts that distinguished them from normal cardiac myofibroblasts. The present study aimed to assess the presence of PADs, the extracellular matrix (ECM), and citrullination in DCM spheroids using immunofluorescence stai

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Induced Pluripotent Stem Cell-Derived Fibroblasts Efficiently Engage Senescence Pathways but Show Increased Sensitivity to Stress Inducers.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

The risk of aberrant growth of induced pluripotent stem cell (iPSC)-derived cells in response to DNA damage is a potential concern as the tumor suppressor genes TP53 and CDKN2A are transiently inactivated during reprogramming. Herein, we evaluate the integrity of cellular senescence pathways and DNA double-strand break (DSB) repair in Sendai virus reprogrammed iPSC-derived huma

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Combined treatment with cetuximab and STA9090 has synergistic anticancer effects on human non-small cell lung cancer.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Cetuximab (CET), a human murine chimeric IgG monoclonal antibody and an inhibitor of epidermal growth factor receptor (EGFR), has been shown to be effective in treating various types of cancer. However, its use is hindered by limitations such as resistance development, variability in patient response, side effects, and challenges in biomarker identification. Therefore, CET is o

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Pro-Oxidant Auranofin and Glutathione-Depleting Combination Unveils Synergistic Lethality in Glioblastoma Cells with Aberrant Epidermal Growth Factor Receptor Expression.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Glioblastoma (GBM) is the most prevalent and advanced malignant primary brain tumor in adults. GBM frequently harbors epidermal growth factor receptor (EGFR) wild-type (

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Exploiting residual cocoa biomass to extract advanced materials as building blocks for manufacturing nanoparticles aimed at alleviating formation-induced oxidative stress on human dermal fibroblasts.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

The global adoption of by-product valorisation processes aligns with the circular economy framework, ensuring sustainability in the agricultural sector. In cocoa production, residual biomass can offer the opportunity to extract advanced materials, contributing to nanotherapeutic solutions for biomedical applications. This study explores extraction processes for valorising cocoa

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Epidermal growth factor augments the self-renewal capacity of aged hematopoietic stem cells.

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Hematopoietic aging is associated with decreased hematopoietic stem cell (HSC) self-renewal capacity and myeloid skewing. We report that culture of bone marrow (BM) HSCs from aged mice with epidermal growth factor (EGF) suppressed myeloid skewing, increased multipotent colony formation, and increased HSC repopulation in primary and secondary transplantation assays. Mice transpl

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Three-Dimensional Cell Culture Micro-CT Visualization within Collagen Scaffolds in an Aqueous Environment.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Among all of the materials used in tissue engineering in order to develop bioequivalents, collagen shows to be the most promising due to its superb biocompatibility and biodegradability, thus becoming one of the most widely used materials for scaffold production. However, current imaging techniques of the cells within collagen scaffolds have several limitations, which lead to a

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Co-Delivery of Valsartan and Metformin from a Thermosensitive Hydrogel-Nanoparticle System Promotes Collagen Production in Proliferating and Senescent Primary Human Dermal Fibroblasts.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Aging negatively impacts skin health, notably through the senescent cell phenotype, which reduces collagen production and leads to thinner, more fragile skin prone to injuries and chronic wounds. We designed a drug delivery system that addresses these age-related issues using a hybrid hydrogel-nanoparticle system that utilizes a poly(δ-valerolactone-

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Influence of mesenchymal and biophysical components on distal lung organoid differentiation.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Chronic lung disease of prematurity, called bronchopulmonary dysplasia (BPD), lacks effective therapies, stressing the need for preclinical testing systems that reflect human pathology for identifying causal pathways and testing novel compounds. Alveolar organoids derived from human pluripotent stem cells (hPSC) are promising test platforms for studying distal airway diseases l

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Hypothermically Stored Amnion Is Robust and Provides a Scaffold for Supporting Wound Healing by Retaining the Characteristics of Native Tissue.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Placental-derived products have been used since the early 1900s for wound applications and have shown clinical utility in supporting wound healing. A hypothermically stored amniotic membrane (HSAM) was developed using a proprietary process to allow for the retention of the extracellular matrix (ECM), viable cells, and key proteins. To evaluate its utility, we characterized the

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Bioprinted High-Cell-Density Laminar Scaffolds Stimulate Extracellular Matrix Production in Osteochondral Co-Cultures.

Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Many tissues have a laminar structure, but there are limited technologies for establishing laminar co-cultures for in vitro testing. Here, we demonstrate that collagen-alginate-fibrin (CAF) hydrogel scaffolds produced using the reactive jet impingement bioprinting technique can produce osteochondral laminar co-cultures with well-defined interfaces between cell types and high ce

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Water-Dispersible and Biocompatible Polymer-Based Organic Upconversion Nanoparticles for Transdermal Delivery.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Photomedicine, which utilizes light for therapeutic purposes, has several hurdles such as limited tissue penetration for short-wavelength light and inadequate deep tissue efficacy for long-wavelength light. Photon energy upconversion (UC) reveals promise in photomedicine because it enables the conversion of lower-energy photons into higher-energy photon. Lanthanide (Ln)-based i

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An Integrated Dual-Layer Heterogeneous Polycaprolactone Scaffold Promotes Oral Mucosal Wound Healing through Inhibiting Bacterial Adhesion and Mediating HGF-1 Behavior.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Recently, the high incidence of oral mucosal defects and the subsequent functional impairments have attracted widespread attention. Controlling scaffold geometry pattern has been proposed as a strategy to promote cell behavior and facilitate soft tissue repair. In this study, we innovatively construct an integrated dual-layer heterogeneous polycaprolactone (PCL) scaffold using

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Systemic factors associated with antler growth promote complete wound healing.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Deer antlers are the only mammalian appendages that can fully regenerate from periosteum of pedicles (PP). This regeneration process starts from regenerative healing of wounds. Removal of PP abolishes antler regeneration, however, the regenerative cutaneous wound healing proceeds, indicating that some factors in the circulation contribute to this healing. In this study, we prod

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Wild Birds' Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem Cells.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Follicular cells represent a valuable resource for genetic research, biotechnology and cryopreservation in biobanks, particularly for the conservation of endangered species. They offer a more practical alternative to gametes, embryos and fibroblasts. Collection of these cells can be achieved through feather plucking. Feather samples were opened with a scalpel and the feather pu

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Light-Intensity-Dependent Control of Collagen Hydrogel Properties via Riboflavin Phosphate-Mediated Photocrosslinking.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

While photocrosslinked collagen hydrogels show promise in tissue engineering, conventional approaches for property control often require complex chemical modifications or concentration changes that alter their biochemical composition. Here, we present the first systematic investigation of light-intensity-dependent control in riboflavin phosphate (RFP)-mediated photocrosslinking

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Utilizing miR-34a-Loaded HER2-Targeting Exosomes to Improve Breast Cancer Treatment: Insights From an Animal Model.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Exosomes, nanoscale vesicles with high biocompatibility, were engineered to express human epidermal growth factor receptor 2 (HER2)-binding peptides and carry miR-34a, targeting HER2 and programmed death-ligand 1 (PD-L1)-positive breast cancer cells. An Intravenous administration of miR-34a-loaded HER2-targeting exosomes, referred to as tEx[34a], demonstrated superior targetabi

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Three-Dimensional Printing of Hydrogel as Skin Substitute and Comparative Evaluation of Melanin Production.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Cell culture in two dimensions has been the main instrument in cellular and molecular biology. But there are limitations to two-dimensional culture when it comes to tissue engineering and in vivo reproduction. Tissue engineering technology enabled the creation of biomedical scaffolds, which are mostly utilized to biofabricate different artificial human organs. Tissue architectu

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Liver portal fibroblasts induce the functions of primary human hepatocytes in vitro.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

In vitro human liver models are critical to mitigate species-specific differences observed for toxicology, disease modeling, and regenerative medicine. Interactions with mesenchyme (i.e., fibroblasts) can promote phenotypic functions of primary human hepatocytes (PHHs) in culture; however, using liver-derived fibroblasts remains elusive. Portal fibroblasts (PFs) around the port

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Dietary Insulinogenic Amino Acid Restriction Improves Glucose Metabolism in a Neonatal Piglet Model.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

<b>Background:</b> Dietary consumption of insulinogenic amino acids (IAA) is known to contribute to the development of insulin resistance. It remains to be studied whether dietary IAA restriction improves glucose metabolism and insulin sensitivity and whether this improvement is related to alterations in glucose metabolism in peripheral tissues. The objective of this study was

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Engineering a Human-Sized Common Bile Duct Prototype with Regenerative Potential: In Vitro Evaluation of Mechanics, Function, Degradation, and Immune Modulation.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Tissue engineering offers new hope for treating biliary defects. Several scaffolds have been proposed, but their properties have not been fully investigated. In this study, the design, fabrication, and characterization of a novel common bile duct (CBD)-like prototype are described. This prototype combines two biocompatible biomaterials, methacrylated type I collagen (CollMA) an

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Biomimetic, Suturable, and Extensible Electrospun Scaffolds for Lower Urinary Tract Surgical Reconstruction.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Common autologous tissue sources used for the repair or replacement of lower urinary tract (LUT) organs include the inner cheek (buccal), small and large bowels, and foreskin. These sources have limited availability, introduce the risk of donor site complications, and differ mechanically and biologically from LUT tissue. To address this, fibrous composite scaffolds with varying

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Synergistic Combinations of Native Australian Plants For Skin Inflammation and Wound Healing.

Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

<b>Background:</b> Inflammation and oxidative stress are key mechanisms in underlying skin conditions like psoriasis and eczema. While many plants, including Australian native plants, are proposed to target these pathways due to their phytochemical content, studies on whole extracts and their synergistic effects remain limited. <b>Objectives</b>: This study aimed to investigate

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Cooling-Triggered Release of Celecoxib from Implantable Alginate-Soluplus Composite Devices.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Currently, on-demand treatment of pain (both chronic and acute) is primarily achieved using opioids that are delivered systemically. Unfortunately, these drugs are highly addictive; over 5 people per hour die from opioid abuse in the US alone. A safer, nonsystemic mechanism for pain relief is therefore needed. Nonsteroidal anti-inflammatory drugs (NSAIDs) have been explored for

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Inflammatory modulation by cord blood stem cells prevented digit deformation in recessive dystrophic epidermolysis bullosa.

Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Recessive dystrophic epidermolysis bullosa (RDEB) is a hereditary dermal blistering disorder caused by mutations in the COL7A1 gene encoding type VII collagen (C7), which progressively results in poor wound healing, fibrosis, and pseudosyndactyly. Using a C7 hypomorphic mouse model of RDEB, we demonstrated that inflammation critically drives disease progression and identified p

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Gallic acid released by a layered double hydroxide-coated scaffold of hydroxyapatite and β-tricalcium phosphate inhibits the osteoclast formation

Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Following dental extraction, alveolar bone loss, driven by the osteoclast (OC) bone-eroding cells, is a relevant concern in dental practice since it could compromise the possibility of installing dental implants. This study aimed to develop a drug delivery system releasing the antiosteoclastogenic molecule gallic acid (GA) at the alveolar bone level to control the dysregulated

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Keratinocyte Autophagy-Mediated Self-Assembling Tetrahedral Framework Nucleic Acid Induces Wound Healing and Reduces Scar Hyperplasia.

Cell type: Keratinocyte | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Tetrahedral framework nucleic acid (tFNA) efficiently treats various diseases; however, its effect on wound healing is unknown. We investigated tFNA's impact on human immortalized epidermal cells (HaCaT) cells and wound healing through in vitro and in vivo experiments. The tFNA is taken up by cells and exhibits good biocompatibility. Transmission electron microscopy and autopha

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Technique for decellularization and characterization of rat esophageal extracellular matrix for potential application as a 3D scaffold.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Conventional treatment for esophageal defects involves surgical removal of the defect area and implant conduit tissues. There exist morbidities and mortalities associated with the treatment including fistula and leakage leading to compromise in quality of life. The aim of this study was to optimize a method for complete decellularization of rat esophagus and to solubilize the d

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Coenzyme-based elastomeric adhesive patch as iron ion capturer to regulate iron metabolism for diabetic oral ulcer repair.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Iron metabolism imbalance under high-glucose and nutrient-rich conditions is a key factor inhibiting the healing of diabetic oral ulcers. This study confirms the extensive iron accumulation and ferroptosis occurrence in diabetic oral ulcers. To address this, an oral patch (PLTP) is designed by combining two natural bioactive molecules of lipoic acid (LA) and tea polyphenols (TP

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Transparent Multifunctional Wearable Strain Sensor With Self-Healing and Antibacterial Capabilities for Human Motion Detection.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Wearable strain sensors are highly desirable due to their increasing applications in smart electronic skins and healthcare monitoring systems. Nevertheless, simultaneously integrating high stretchability, sensing linearity, stable operation under sub-zero temperatures, and long-term storage for conductive films remains a formidable challenge. Herein, a dual-network polyvinyl al

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Fibroblast-loaded carboxymethyl chitosan-aldehyde hyaluronic acid injectable hydrogel for scleral remodelling to prevent development of myopia.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

High myopia severely threatens the visual health of adolescents, with pathological features of decreased collagen aggregation and scleral thinning, ultimately leading to axial elongation on preretinal imaging. Fibroblasts play crucial roles in scleral remodelling and myopia prevention. In this work, we developed a fibroblast-loaded carboxymethyl chitosan-aldehyde hyaluronic aci

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Keratin Additive for Cellular Adhesion in Transcutaneous Prosthetics.

Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

The dermal barrier is widely considered the body's first line of defense against most foreign bodies, protecting it from both moisture loss and bacterial invasion. However, when the skin is ruptured for long-term medical interventions (e.g., transcutaneous prosthetics), it is difficult to restore and maintain this protective barrier. Although there are no direct, biological exa

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Monovalent pseudo-natural products supercharge degradation of IDO1 by its native E3 KLHDC3.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Targeted protein degradation modulates protein function beyond the inhibition of enzyme activity or protein-protein interactions. Most degrader drugs function by directly mediating the proximity between a neosubstrate and a hijacked E3 ligase. Here we identify pseudo-natural products derived from (-)-myrtanol, termed iDegs, that inhibit and induce degradation of the immunomodul

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Wound healing and photodynamic potential of

Cell type: Keratinocyte | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Chronic and infected wounds represent a persistent global health burden. Medicinal plants offer a promising source of wound-healing agents due to their multitarget activities, long history of traditional use, and accessibility. We evaluated the effects of methanol extracts of ACV (ACVM) on cell proliferation, migration and antioxidative capacity in human keratinocytes (HaCaT),

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Mosaic partial epidermal reprogramming remodels neighbors and niches to refine skin homeostasis and repair.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Adult stem cells and their niches communicate intricately for tissue maintenance and regeneration. However, effectively coordinating these complex interactions is challenging. Here, we demonstrate that transient dedifferentiation of a fraction of epithelial stem cell progenies orchestrates beneficial changes within the entire skin's cellular networks to favor repair. We achieve

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Identification and Functional Characterization of Cytoprotective Crocodile-Derived Peptides against Oxidatively Stressed Human Keratinocytes.

Cell type: Keratinocyte | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Oxidative stress-induced cellular damage is a primary contributor to pathological conditions such as accelerated skin aging. Consequently, there is a strong demand for efficacious antioxidants. Herein, we investigate the cytoprotective properties of

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The transcription elongation factors Spt4 and Spt6 promote dermal adipocyte differentiation.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Regulation of adipogenesis has classically been viewed through the lens of transcription initiation driven by lineage defining transcription factors. In this study, we uncover transcription elongation as a critical and previously underappreciated regulatory layer controlling adipocyte cell fate. We demonstrate that the elongation factors Spt4 and Spt6 are indispensable for adip

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Integrin-Binding Matricellular Protein Fibulin-5 Maintains Epidermal Stem Cell Heterogeneity During Skin Aging.

Cell type: Keratinocyte | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

The extracellular matrix (ECM) is crucial in building the extracellular environment and translating extracellular information into biochemical signals that sustain tissue functions. Fibulin-5 (Fbln5) is a multifunctional ECM protein essential for forming elastic fibers and regulating cellular functions by binding to integrins. Although fibulin-5 expression decreases with age in

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Mechanical Reliability and Biocompatibility of Graphene-Modified Photocurable Resins for Additive Manufacturing: Implications for Potential Biomedical Use.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Additive manufacturing using stereolithography enables the fabrication of intricate small-scale parts, making it ideal for biomedical applications such as prostheses and scaffolds. This study evaluates the mechanical reliability and biocompatibility of photocurable acrylic resins modified with graphene-based nanomaterials, graphene (G) and graphene oxide (GO), to address limita

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Evaluation of polyethyleneimine-functionalized cellulose for the capture of valeric acid, a model wound malodor compound.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

Short-chain fatty acids (SCFAs), such as valeric acid, are key contributors to malodor in chronic wounds, yet conventional sorbents fail to retain these volatile compounds effectively. To address this, polyethyleneimine-functionalized cellulose (PFC) was developed as a reactive sorbent for selective SCFA capture via ionic interactions between protonated surface amines and disso

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Engineering a 3D matrix from porcine plasma for enhanced cell proliferation and differentiation.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Skin, Wound & Biomaterials

The limited availability of suitable scaffolds for three-dimensional (3D) cell culture has driven the development of new biomaterials. In this study, we developed a porcine fibrin matrix gel (PFMG) derived entirely from porcine blood as a species-matched 3D scaffold that integrates the structural support of fibrin with the bioactive components of porcine platelet lysate (PPL).

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