EVOS M5000 in Cancer & Oncology: Independent Studies — Wave 3 Part 3

Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.

EVOS M5000IndependentWave 3

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Selisistat, a SIRT1 inhibitor, enhances paclitaxel activity in luminal and triple-negative breast cancer: in silico, in vitro, and in vivo studies.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Sirtuins (SIRTs) are NAD+-dependent histone deacetylases, which play a key role in cancer progression; however, their prognostic values in breast cancer (BC) remain a subject of debate and controversy. Accumulative evidence suggests that each sirtuin possesses individual character, implicating its role in the regulation of multifaceted biological functions leading to BC initiat

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Targeting Chk1 and Wee1 kinases enhances radiosensitivity of 2D and 3D head and neck cancer models to X-rays and low/high-LET protons.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Ionising radiation causes the introduction of DNA damage, more specifically double strand breaks (DSBs) and complex DNA damage (CDD), that induces cancer cell death leading to the therapeutic effect. To combat this, cells activate arrest at the G

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Brazilian Stingless Bee Geopropolis Exhibit Antioxidant Properties and Anticancer Potential Against Hepatocellular Carcinoma Cells.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Hepatocellular carcinoma (HCC) is the third most common cancer in terms of mortality and the sixth in incidence worldwide. Treatment varies by tumor stage, but low survival rates are common across all stages. Due to these poor outcomes, there is a critical need for new treatment options and lead compounds, prompting an active search. Geopropolis has been identified as a source

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Boosting tumor homing of endogenous natural killer cells via therapeutic secretomes of chemically primed natural killer cells.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Natural killer (NK) cells play a critical role in modulating immune responses by secreting soluble factors, including chemotactic cytokines. Our previous study demonstrated the potent antitumor activity of Chem_NK, referring to NK cells chemically primed with 25 kDa branched polyethyleneimine. However, the potential of Chem_NK secretomes to educate other NK cells and enhance th

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The essential clathrin adapter protein complex-2 is tumor suppressive specifically in vivo.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

The microenvironment is a rich source of new cancer targets. We thus used a targeted single-guide RNA library to screen a panel of human pancreatic cancer lines for genes uniquely affecting tumorigenesis. Here we show inactivation of the Adapter Protein complex-2 of clathrin-mediated endocytosis reduces cell growth in vitro, but completely oppositely, promotes tumor growth in v

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<sup>64</sup>Cu-chelated InP/ZnSe/ZnS QDs as PET/fluorescence dual-modal probe for tumor imaging.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Positron emission tomography (PET)/fluorescence dual-modal imaging combines deep penetration and high resolution, making it a promising approach for tumor diagnostics. Semiconductor nanocrystals, known as quantum dots (QDs), have garnered significant attention for fluorescence imaging owing to their tunable emission wavelength, high quantum yield, and excellent photostability.

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Defining the Protein Phosphatase 2A (PP2A) Subcomplexes That Regulate FoxO Transcription Factor Localization.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

The family of forkhead box O (FoxO) transcription factors regulate cellular processes involved in glucose metabolism, stress resistance, DNA damage repair, and tumor suppression. FoxO transactivation activity is tightly regulated by a complex network of signaling pathways and post-translational modifications. While it has been well established that phosphorylation promotes FoxO

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Tumor Regulatory Effect of 15-Hydroxyprostaglandin Dehydrogenase (HPGD) in Triple-Negative Breast Cancer.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Prostaglandin regulation is known to play a pivotal role in tumorigenesis; however, the contributions of the prostaglandin-metabolizing enzyme 15-hydroxyprostaglandin dehydrogenase (HPGD) to cancer development remain poorly understood. In this study, we investigate the effects of HPGD on cell viability, proliferation, anchorage-independent growth, and migration in triple-negati

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Dual-pathway tumor radiosensitization strategy based on engineered bacteria capable of targeted delivery of AuNPs and specific hypoxia alleviation.

Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Radiotherapy efficacy remains constrained by two key challenges: dose-dependent toxicity to healthy tissues at high radiation doses and hypoxia-mediated tumor radioresistance. While radiosensitizers like gold nanoparticles can enhance tumor-specific radiation deposition, their targeted delivery to tumors presents a significant hurdle. Bacteria have emerged as promising bio-carr

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Inhibition of PINK1 senses ROS signaling to facilitate neuroblastoma cell pyroptosis.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Mitochondria serve as the primary source of intracellular reactive oxygen species (ROS), which play a critical role in orchestrating cell death pathways such as pyroptosis in various types of cancers. PINK1-mediated mitophagy effectively removes damaged mitochondria and reduces detrimental ROS levels, thereby promoting cell survival. However, the regulation of pyroptosis by PIN

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Combined Omipalisib and MAPK Inhibition Suppress PDAC Growth.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

<b>Background</b>: Oncogenic KRAS mutations are nearly ubiquitous in pancreatic ductal adenocarcinoma (PDAC), yet therapeutic attempts to target KRAS, as well as downstream MAPK pathway effectors, have shown limited clinical success. While KRAS canonically drives MAPK signaling via RAF-MEK-ERK, it is also known to play a role in PI3K-AKT signaling. <b>Methods</b>: Our therapeut

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Wireless charging LED mediated type I photodynamic therapy of breast cancer using NIR AIE photosensitizer.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Due to limited light penetration and dependence on oxygen, photodynamic therapy (PDT) is typically restricted to treating shallow tissues. Developing strategies to overcome these limitations and effectively using PDT for tumor treatment is a significant yet unresolved challenge. In this study, we present a smart approach combining a wireless-charged LED (wLED) with a type I agg

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Loss of VHL-mediated pRb regulation promotes clear cell renal cell carcinoma.

Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

The von Hippel-Lindau (VHL) tumor suppressor is a substrate-defining component of E3 ubiquitin ligase complexes that target cellular substrates for proteasome-mediated degradation. VHL inactivation by mutation or transcriptional silencing is observed in most sporadic cases of clear cell renal cell carcinoma (ccRCC). VHL loss in ccRCC leads to constitutive stabilization of E3 li

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Kinin B

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

<b>Background/Objectives</b>: The low permeability of the blood-brain barrier (BBB) represents a significant challenge to effective systemic chemotherapy for primary and metastatic brain cancers. Kinin receptors play a crucial role in modulating BBB permeability, and their agonist analogs have been explored in preclinical animal models to enhance drug delivery to the brain. In

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PLK4 Inhibition as a Strategy to Enhance Non-Small Cell Lung Cancer Radiosensitivity.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Lung cancer is the leading cause of cancer-related mortality worldwide. Non-small cell lung cancer (NSCLC) is the most common subtype of lung cancer and comprises 85% of cases. Despite treatment advances, local control after curative-intent chemoradiation for NSCLC remains suboptimal. Polo-like kinase 4 (PLK4) is a serine-threonine kinase that plays a critical role in the regul

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Targeted Cancer Therapy with Gold-Iron Oxide Nanourchins: Inducing Oxidative Stress, Paraptosis, and Sensitizing Tumor Cells to Cisplatin.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Nanotechnology has revolutionized cancer therapy by enabling targeted drug delivery and overcoming limitations associated with conventional chemotherapy. In this study, we explored the anticancer potential of gold-iron oxide (Au-Fe

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TOX High-Mobility Group Box Family Member 4 promotes DNA double-strand break repair via nonhomologous end joining.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Nonhomologous end joining (NHEJ) is a pivotal mechanism in the repair of DNA double-strand breaks. Central to NHEJ is the DNA-dependent protein kinase (DNA-PK) complex, comprising the KU heterodimer and the catalytic subunit, DNA-PKcs. In this study, we characterize Thymocyte Selection-Associated High-Mobility Group Box Family Member 4 (TOX4) as a factor recruited to both laser

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Modulation of HER2 internalization enhances single-dose antibody-drug potency in HER2

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

HER2 is a membrane receptor tyrosine kinase overexpressed in 18-20% of gastric tumors. Trastuzumab emtansine (T-DM1) is an antibody-drug conjugate (ADC) that targets HER2-positive (HER2

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New spirooxindole pyrrolidine/pyrrolizidine analogs: design, synthesis, and evaluation as an anticancer agent.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

A novel series of mesitylene-based spirooxindoles were synthesized

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YAP1 promoter-associated noncoding RNA affects Ewing sarcoma cell tumorigenicity by regulating YAP1 expression.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Ewing sarcomas (ESs) are aggressive paediatric tumours of bone and soft tissues afflicting children and adolescents. Despite current therapies having improved the 5-year survival rate to 70% in patients with localized disease, 25% of patients relapse and most have metastasis at diagnosis. Resistance to chemotherapy, together with the high propensity to metastasize, remain the m

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Cancer therapy via neoepitope-specific monoclonal antibody cocktails.

Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Cellular heterogeneity presents a significant challenge to cancer treatment. Antibody therapies targeting individual tumor-associated antigens can be extremely effective but are not suited for all patients and often fail against tumors with heterogeneous expression as tumor cells with low or no antigen expression escape targeting and develop resistance. Simultaneously targeting

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EGFRvIII-driven microenvironmental fibroblast activation and transformation accelerate oral cancer progression via lipocalin-2/STAT3 axis.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Oral squamous cell carcinoma (OSCC) is an aggressive malignancy frequently characterized by dysregulated epidermal growth factor receptor (EGFR) signaling. Among EGFR mutation, EGFRvIII, an extracellular domain truncated form without exons 2-7, exhibits ligand-independent and constitutive EGFR activation. Although EGFRvIII functions as an oncogene in glioblastoma, its role in O

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The Interplay of SMAD4 and EMT in Oral Squamous Cell Carcinoma.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

<b>Background:</b> Oral squamous cell carcinoma (OSCC) is a prevalent malignancy with a poor prognosis. Surgical removal of the primary tumor and regional lymph nodes (LNs) remains the fundamental treatment for OSCC, although 40% of patients are negative for LN metastasis. The epithelial-mesenchymal transition (EMT) plays a crucial role in OSCC progression by enabling epithelia

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Arylsulfatase B induces melanoma apoptosis by the ubiquitin ligase COP1.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Previous experiments in the syngeneic, murine, and subcutaneous model of malignant melanoma and human melanoma cells showed that treatment by recombinant human (rh) Arylsulfatase B (ARSB; N-acetylgalactosamine-4-sulfatase) markedly reduced the volume of tumors, improved survival, and inhibited invasiveness. In this report, the impact of ARSB on the progression of metastatic, pu

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Comparative Analysis of MBNL1 Antibodies: Characterization of Recognition Sites and Detection of RNA Foci Colocalization.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

<b>Background/Objectives:</b> MBNL1 is an RNA-binding protein involved in RNA metabolism, including splicing. It colocalizes with RNA foci, a pathological hallmark of myotonic dystrophy, and plays a central role in its disease mechanism. Moreover, MBNL1 has been implicated in other neuromuscular disorders and cancers. In these pathological and biochemical studies, the detection

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TMBIM6 promotes glioma progression according to integrated bioinformatics and experimental evidence.

Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

TMBIM6, a transmembrane BAX inhibitor motif containing 6, located in the endoplasmic reticulum, is linked to various cellular processes and cancer progression. Previous investigations showed a substantial association between TMBIM6 and survival in patients diagnosed with various cancer types. However, the lack of extensive studies addressing the correlation between TMBIM6 and g

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cJun-N-terminal kinase activity and mitosis perturbation drive the 2-methoxyestradiol-mediated enhancement of viral oncolysis by Epizootic Hemorrhagic Disease Virus-Tel Aviv University.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Oncolytic viruses (OVs) are promising cancer therapies whose efficacy is restricted by tumor heterogeneity and monotherapy limitations. We investigated combining the oncolytic orbivirus Epizootic Hemorrhagic Disease Virus -Tel Aviv University (EHDV-TAU) with the microtubule-targeting agent (MTA) 2-methoxyestradiol (2ME2) for the oncolysis of bladder cancer (BC) cells (T24 or SW

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Simvastatin suppresses spinal cord metastasis of medulloblastoma at clinically significant doses.

Cell type: B cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Medulloblastomas (MBs) are aggressive brain cancers and represent the most common primary malignant tumour in children. Current treatment protocols involve an intensive regimen of surgery, radiation therapy and chemotherapy, guided by histopathology and risk stratification. Unfortunately, disease relapse proves fatal in 30% of cases, and treatment efficacy is compromised as MB

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Structure-Based Discovery of Hsp90/HDAC6 Dual Inhibitors Targeting Aggressive Prostate Cancer.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

HDAC6 and Heat Shock Protein 90 (Hsp90) are key regulators within the androgen response pathway, exhibiting a close interplay and mutual interaction patterns that make their combined inhibition a promising strategy for treating aggressive prostate cancer (PC). Herein, we present the structure-based design of dual inhibitors of Hsp90 and HDAC6 that leveraged the crystal structur

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The T-Box Transcription Factors TBX2 and TBX3 Are Molecular Targets of Piroctone Olamine in the Treatment of Pancreatic Cancer.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Pancreatic ductal adenocarcinoma (PDAC) has a poor 5-year survival rate of < 10% and its incidences are continuously rising worldwide. This highlights an urgent need for effective therapies to reduce its burden. Repurposing commercially available non-cancer drugs that inhibit key drivers of PDAC may facilitate the rapid identification of effective drugs. In PDAC, the expression

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Co-Targeting BCL-xL with MCL-1 Induces Lethal Mitochondrial Dysfunction in Diffuse Mesothelioma.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Diffuse mesothelioma is a rare but highly aggressive and treatment-resistant neoplasm with low survival rates. Effective therapeutic strategies are limited, and resistance to treatment is a major obstacle. Myeloid cell leukemia (MCL)-1 and B-cell leukemia (BCL)-xL are antiapoptotic B-cell lymphoma 2 (Bcl-2) family proteins that block cell-intrinsic apoptosis through interaction

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Cerium oxide nanoparticles prepared through Bio-combustion using Ficus carica as effective antioxidant, anticancer and dye degrading agent.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

The rising levels of environmental contamination and oxidative stress disorders have led to a growing demand for multifunctional nanomaterials that possess both biomedical and catalytic importance. CeO

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DKC1-mediated pseudouridylation of rRNA targets hnRNP A1 to sustain IRES-dependent translation and ATF4-driven metabolic adaptation.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

The pseudouridine synthase DKC1 regulates internal ribosome entry site (IRES)-dependent translation and is up-regulated in cancers by the MYC family of oncogenes. The functional significance of DKC1 up-regulation and the mechanistic connection between pseudouridylation and IRES-mediated translation remain poorly understood. Here, we report that DKC1 drives an ATF4-mediated tran

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FASN promotes the stemness of cancer stem cells and protects colorectal cancer cells from ferroptosis by inhibiting the activation of SREBP2.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Fatty acid synthase (FASN) is a key regulator of lipid metabolism, but its role in colorectal cancer (CRC) stemness and ferroptosis remains unclear. FASN expression in CRC was analyzed using TCGA data and validated in CRC cell lines (CACO-2, HCT116, SW480) and normal HIEC-6 cells via qRT-PCR and Western blot. HCT116 cells (highest FASN expression) were used for experiments. FAS

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Basal cell of origin resolves neuroendocrine-tuft lineage plasticity in cancer.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Neuroendocrine and tuft cells are rare chemosensory epithelial lineages defined by the expression of ASCL1 and POU2F3 transcription factors, respectively. Neuroendocrine cancers, including small cell lung cancer (SCLC), frequently display tuft-like subsets, a feature linked to poor patient outcomes

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Adaptaquin is selectively toxic to glioma stem cells through disruption of iron and cholesterol metabolism.

Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Glioma stem cells (GSCs) from this aggressive brain cancer have been subject to nononcogene addiction therapeutic strategies, in particular targeting iron and cholesterol metabolic pathways. In this study, we show the small molecule Adaptaquin (AQ) has anti-GSC effects while sparing neurons, mature oligodendrocytes and astrocytes. Transcriptomic analysis of AQ-treated GSCs show

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Isolation, Extraction, and Analysis of Cells After Confined Migration.

Cell type: T cell | Technique: Fluorescence imaging, live-cell | Disease/area: Cancer & Oncology

Cell migration through confined microenvironments is a critical biological process that underlies numerous physiological and pathological events, including immune cell trafficking, tissue morphogenesis, and cancer metastasis. Although polydimethylsiloxane-based microchannel devices have enabled detailed studies of confined migration, the efficient collection of cells post-migra

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Inhibition of the RAC/PAK Signaling Axis Enhances the Potency of MAPK Cascade Inhibitors Against Uveal Melanoma.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Uveal melanoma is a melanocyte-derived malignancy of the eye with a high propensity for liver metastasis. Metastatic uveal melanoma is associated with high mortality and is poorly responsive to currently available therapies. Most uveal melanoma cases are driven by activating mutations in GNAQ and GNA11 genes, which convey oncogenic signaling through the mitogen-activated protei

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Targeting Osteosarcoma: The Dual Action of Halogenated Boroxine and Cerium Oxide Nanoparticles.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Current standard treatments for osteosarcoma have not been changed for decades and have limited and variable success. The advancement of precision medicine technologies, along with the drug-repurposing and fast drug-screening methodologies available, has opened new avenues for the development of more effective therapeutic strategies. In this study, we evaluated the effectivenes

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miR-124 Targets EGFR and Attenuates Growth and Invasion in Bladder Cancer Cells.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

The epidermal growth factor receptor (EGFR) is a key driver in bladder cancer progression. This study investigated the tumor-suppressive role of miR-124-3p and its regulatory effect on EGFR. TSGH8301 and T24 bladder cancer cells were treated with the EGFR inhibitor erlotinib or transfected with an miR-124-3p mimic. Cell viability, proliferation, migration, and invasion were ass

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CCR2-driven monocyte recruitment is protective against radiotherapy-induced intestinal toxicity.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Radiotherapy (RT) is essential in treating abdominal and pelvic cancers but often damages the healthy tissues, particularly the intestines, leading to radiation-induced toxicities with limited treatment options. While the immune system is known to help regulate tissue damage, immune mechanisms involved in RT-induced intestinal toxicity are not fully understood. Following CT-gui

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RUNX2 cooperates with SREBP1 to rewire cancer metabolism and promote aggressiveness.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Embryonic Transcription Factors (TFs) are often reactivated in cancer, driving developmental gene programs that support phenotypic plasticity. Metabolic adaptation fuels this plasticity by supplying energy and molecular building blocks for growth. RUNX2, the master regulator of bone morphogenesis, is ectopically expressed in epithelial cancer, promoting metastasis through trans

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Loss of two-pore channel 2 enhances CD8+ T cell cytotoxicity and directly impairs tumour growth via MAPK axis in HCC.

Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Hepatocellular carcinoma (HCC) remains a major global health challenge, characterised by limited therapeutic options and high mortality rates. Despite significant progress in systemic and immune-based therapies, many patients develop resistance or fail to respond, highlighting the need for new molecular targets. Lysosomal ion channels have recently emerged as important regulato

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Natural Compound Melatonin Suppresses Breast Cancer Development by Regulating Circadian Rhythm.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

<b>Background:</b> Breast cancer remains a major global health threat to women. While current therapies exist, their limitations necessitate novel strategies. Melatonin, an endogenous circadian regulator, has shown anti-tumor potential, but its mechanisms from a circadian perspective require further exploration. <b>Methods:</b> The anti-tumor effects of melatonin were evaluated

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Targeting cystatin F activation enhances NK cell cytotoxicity in glioblastoma models.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Glioblastoma (GBM) is a highly invasive brain tumor with limited treatment options and poor prognosis. Natural killer (NK) cells are key effectors of antitumor immunity, capable of eliminating cancer stem-like cells. However, GBM creates an immunosuppressive microenvironment that limits NK cell function. Here, we identify cystatin F as an immunosuppressive factor involved in re

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Adverse pro-tumorigenic effects of IDO1 catalytic inhibitors mediated by the non-enzymatic function of IDO1 in tumor cells.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Indoleamine 2,3-dioxygenase 1 (IDO1) inhibitors have been developed with the aim of reinvigorating antitumor T-cell responses in the tumor microenvironment by blocking the conversion of the essential amino acid tryptophan into immunoregulatory kynurenines. The lack of efficacy demonstrated in the clinical trials prompts us to revise the "on-target" mechanism of these molecules.

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Sulphonamide derivatives with a triazine core as novel inducers of apoptosis and pyroptosis in glioblastoma multiforme cells.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Glioblastoma multiforme (GBM) is a highly aggressive brain tumour with few effective treatment options. This study evaluated two novel triazine-based sulphonamides, MM118 and MM119, for their anticancer effects on GBM cells.

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Binimetinib and encorafenib for the treatment of advanced solid tumors with non-V600E BRAF mutations: results from the Phase II BEAVER trial.

Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Class 2 and 3 non-V600E BRAF mutations are oncogenic drivers in many cancer types. Currently, there are no established targeted therapies with proven efficacy for cancers with non-V600E BRAF mutations. We developed the investigator-initiated, Phase II BEAVER clinical trial (NCT03839342) to evaluate the efficacy of BRAF and MEK inhibitors in patients with non-V600E BRAF mutation

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Human organoid tumor transplantation identifies functional glioblastoma-microenvironment communication mediated by PTPRZ1.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Glioblastoma is the most aggressive and deadly form of brain cancer. Here, we leverage our human organoid tumor transplantation (HOTT) co-culture system to explore how extrinsic cues modulate glioblastoma cell types and behavior. HOTT recapitulates core features of major patient tumor cell types and key aspects of neural cell-enriched tumor microenvironment (nTME) gene programs

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Hepatocellular carcinoma-linked

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

AXIN1 organizes assembly of a destruction complex that degrades the transcriptional co-activator β-catenin, thereby preventing inappropriate Wnt/β-catenin signaling. In hepatocellular carcinoma (HCC),

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Glycolytic heterogeneity drives metabolic-targeted therapy in pancreatic ductal adenocarcinoma.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Pancreatic ductal adenocarcinoma is traditionally characterized as a glycolytic tumor. However, the extent and clinical relevance of its metabolic heterogeneity remain poorly understood. In this study, we investigated whether glycolytic activity follows a consistent expression pattern across pancreatic ductal adenocarcinoma patients and explored how metabolic diversity influenc

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Loss of JAK1 Function Causes G2-M Cell-Cycle Defects Vulnerable to KIF18A Inhibition.

Cell type: Cell culture models | Technique: Fluorescence imaging, live-cell | Disease/area: Cancer & Oncology

Therapeutic resistance to DNA damage is a significant challenge in oncology. To gain insight into the biological mechanisms that cause DNA damage resistance and to inform strategies for achieving synergy with therapeutic radiation, we performed parallel pooled genetic CRISPR-Cas9 screening for survival in high-risk head and neck squamous cell carcinoma (HNSCC) subtypes. Surpris

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HSP90α and KLK6 Coregulate Stress-Induced Prostate Cancer Cell Motility.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Prostate cancer (PCa) metastasis is reliant on the activity of proteases, such as matrix metalloproteinase-2 (MMP-2). While increased extracellular heat shock protein 90α (eHSP90α) has been linked to increased MMP-2 activity, this has not been examined in the context of cellular stress. We examined stress-induced eHSP90α in human prostate cell lines by immunoblot. Fluorometric

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Multi-omics characterization of C4orf19 in HNSCC: constructing prognostic signatures for immunotherapy and chemotherapy response prediction.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

BACKGROUNDS: C4orf19, a protein encoded by an open reading frame on human chromosome 4, exhibits aberrant expression in various tumors and is linked to patient prognosis, though its role in head and neck squamous cell carcinoma (HNSCC) remains unclear. METHODS: TCGA and GEO datasets were analyzed to evaluate the prognostic value of C4orf19 in HNSCC. Functional assays (EdU, immu

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Cellular Identity Crisis: RD3 Loss Fuels Plasticity and Immune Silence in Progressive Neuroblastoma.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology

Therapy-resistant neuroblastoma (NB) reflects a lethal convergence of cellular plasticity and immune escape, yet the molecular drivers remain elusive. Here, we identify retinal degeneration protein 3 (RD3) as a master regulator of NB lineage fidelity and tumor immunogenicity. RD3 loss, induced by therapy, triggers a reprogramming cascade involving epithelial-mesenchymal transit

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