EVOS M5000 in Cancer & Oncology: Independent Studies — Wave 3 Part 4
Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.
EVOS M5000IndependentWave 3
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Impact of neuroendocrine neoplasm-specific systemic treatments on expression and function of CXCR4 in neuroendocrine tumor cells.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
As neuroendocrine neoplasms (NEN) undergo increasing dedifferentiation, CXC chemokine receptor type 4 (CXCR4) becomes upregulated. Higher levels of CXCR4 are associated with invasive growth, metastasis formation, and poor prognosis. CXCR4 is considered a potential diagnostic biomarker and a promising target for precision therapies - e.g., endoradiotherapeutic approaches, monocl
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Hydrogel Microdroplet Based Glioblastoma Drug Screening Platform.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
Glioblastoma is the most common primary malignant brain tumor with a 5-year survival rate < 5%. The standard of care involves surgical resection followed by treatment with the alkylating agent temozolomide (TMZ). GBM cells that evade surgery eventually become resistant to TMZ and lead to recurrence of tumors in patients. With only four drugs currently FDA-approved for GBM treat
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Mechanism of IL-4 mediated RPL19 promoting malignant progression in HER2 positive breast cancer.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
BACKGROUND: Despite recent improvements in prognosis, breast cancer remains the leading cause of cancer-related mortality in women worldwide. Survival rates vary across breast cancer subtypes. HER2, an oncogene, is amplified in approximately 25% of primary breast cancers and associated with an aggressive clinical course and a poor prognosis. Although HER2-targeted therapies ind
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Doxorubicin enhances adipogenesis in an FGF2-dependent manner and induces a tumour-promoting secretory phenotype.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
The bone marrow microenvironment is highly saturated with bone marrow adipocytes (BMA), which differentiate from their precursor, mesenchymal stem cells (MSC). Evidence from patient trials suggests that bone marrow adiposity is increased in patients following some forms of chemotherapy. Moreover, it has been suggested that BMA can confer chemotherapeutic resistance to tumour ce
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Mitochondrial preservation through the PKA-DRP1 axis empowers natural killer cells to resist acidic stress and retain cytotoxicity.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
BACKGROUND: Natural killer (NK) cells are critical components with potent cytotoxic capabilities in immunosurveillance against cancer. However, their function is often impaired in the acidic tumor microenvironment due to mitochondrial dysfunction. METHODS: Chemically primed NK (Chem_NK) cells were generated with 25 kDa branched polyethylenimine. Cytotoxicity and motility assays
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Connective tissue growth factor contributes to resistance to anti-angiogenic therapies in renal cancer.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
Clear cell renal cell carcinoma (ccRCC) is predominantly treated with anti-angiogenic therapies (AATs), such as sunitinib and axitinib. While these therapies initially improve outcomes, resistance frequently emerges, limiting long-term efficacy. Understanding the molecular mechanisms underlying AAT resistance is essential to optimize treatment strategies. To identify factors in
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Inhibition of ZBTB7B-mediated ADPGK transcription by NEDD4 impedes glycolysis and progression of lung adenocarcinoma.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
Lung adenocarcinoma, the predominant type of non-small cell lung cancer, is associated with poor survival outcomes due to late-stage diagnosis, resistance to therapy, and lack of effective metabolic-targeted strategies. Increased glycolysis is a hallmark of LUAD progression, yet the upstream transcriptional and post-translational regulators of glycolytic enzymes remain incomple
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Chromosomal instability shapes the tumor microenvironment of esophageal adenocarcinoma via a cGAS-chemokine-myeloid axis.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
Chromosomal instability (CIN), a pervasive feature of esophageal adenocarcinoma (EAC), drives tumor aggressiveness and metastasis. CIN stimulates the cGAS-STING pathway, typically linked to antitumor immunity. However, despite the high CIN burden in EAC, the cGAS-STING pathway remains largely intact. To address this paradox, we interrogated multiple esophageal cancer models, fi
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Obesity Accelerates Multiple Myeloma Progression in Certain Mouse Models and in Humans.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
Severe obesity is positively associated with the risk of multiple myeloma and mortality; thus, obesity has emerged as a target for multiple myeloma prevention. Established dietary-based mouse models have proven useful in recapitulating obesity-related diseases in humans, but the multifaceted nature of obesity, cancer, and mouse models has led to a knowledge gap about which myel
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Albumin-Bound STING Agonist Reprograms HSPCs to Antitumor Neutrophils Enhancing CD8
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
Tumor-associated immunosuppressive neutrophils, termed polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs), compromise cancer immunotherapy. Emerging evidence indicates that neutrophil fate can be programmed as early as the hematopoietic stem and progenitor cell (HSPC) stage. Reprogramming HSPCs toward antitumor neutrophils offers a promising therapeutic strategy. He
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Ultrasound-activated nanoregulator enables Cu
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
Modulating intratumoral copper homeostasis to trigger cuproptosis is a promising copper‑based anticancer strategy, but its clinical translation is hindered by the absence of precise spatiotemporal control over intracellular copper levels and ATP7A‑mediated active copper efflux. Here we report an ultrasound-activated copper molybdate nanoregulator, HCu
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Targeting glioblastoma mitochondrial metabolism with S-Gboxin induces cytotoxicity under conditions of the tumor microenvironment.
Cell type: B cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
Glioblastoma (GB) is the most common primary malignant brain tumor in adults. Gboxin, a novel compound that targets oxidative phosphorylation via complex V inhibition, has shown promise in preclinical models of GB. We examined the efficacy of the pharmacokinetically optimized S-Gboxin under conditions replicating the GB microenvironment, including nutrient deprivation and hypox
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Cytokines IL-6, IL-10, and CCL5 Secreted by Infiltrating B Cells Promote Cell Migration of Human Prostate Cancer Cell Lines.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
The progression of prostate cancer cells to metastasis is supported by their tumor microenvironment. Within this microenvironment, infiltrating immune cells, such as B cells, can be either anti-tumorigenic or pro-tumorigenic. Our preliminary data showed that a higher density of the infiltrating B cells was found near prostate cancer cells in human cancer tissues, as compared to
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Single-cell analysis of matched FFPE and frozen tissue samples reveals comparable resolution of intratumoural heterogeneity.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
Intra-tumoural heterogeneity contributes to treatment resistance and disease progression in cancer. Single-cell RNA sequencing (scRNA-seq) enables profiling of cellular diversity in the tumour microenvironment. However, current protocols for generating single-cell 3'gene expression data require intact RNA, which excludes archival formalin-fixed paraffin-embedded (FFPE) tissues.
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The Preclinical Effects and Mechanisms of Biofield Therapy on Pancreatic Cancer Cell Growth and Metastasis.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
The high mortality of pancreatic ductal adenocarcinoma (PDAC) underscores the need for novel treatments. We investigated the potential role of human biofield therapy (BT) in modifying tumorigenic processes in murine and human PDAC cells through a series of in vitro and in vivo studies. Cell viability and ultrastructure changes were examined by PrestoBlue assay and Transmission
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Yeast Phenomic Analysis Reveals DNA Repair, pH Homeostasis, and Ribosomal Biogenesis as Modulators of Anticancer Ruthenium Complex KP1019.
Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
The anticancer ruthenium complex indazolium
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Thrombospondin-1 triggers calreticulin expression in human mucoepidermoid carcinoma MC-3 cells via the PERK/CHOP pathway.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
Mucoepidermoid carcinoma (MEC) is the most common salivary gland malignancy, accounting for ~30% of all salivary gland malignancies; however, effective treatments for advanced-stage disease remain limited. The induction of immunogenic cell death (ICD) has emerged as a potent anti-tumor intervention for MEC. Thrombospondin-1 (TSP-1) exhibits documented anti-tumor properties in M
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Synergistic Effects of IMP-1700, Ciprofloxacin, and X-Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial-Resistant Bacteria.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
IMP-1700 was developed as a compound designed to target bacterial DNA repair-associated pathways, including homologous recombination mediated by the AddAB/RecBCD system. While its antibacterial properties are well established, the potential effects on mammalian noncancerous and tumor cells under genotoxic stress remain to be elucidated. This study investigates the impact of IMP
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Impact of Neuroendocrine Neoplasm-Specific Systemic Treatments on Somatostatin Receptors Expression and Function in Neuroendocrine Tumor Cells.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cancer & Oncology
<b>Background</b>: Somatostatin receptors (SSTRs) are pivotal diagnostic and therapeutic targets in well-differentiated neuroendocrine neoplasms (NENs). SSTR-directed treatment strategies rely on sufficient SSTR2 expression. Thus, receptor loss during dedifferentiation limits therapeutic efficacy. Preclinical data suggest pharmacologic modulation of SSTRs' expression. However,
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