EVOS M5000 in Imaging Techniques & Methods: Independent Studies — Wave 3 Part 1
Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.
EVOS M5000IndependentWave 3
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Laterals take it better - Emerging and young lateral roots survive lethal salinity longer than the primary root in Arabidopsis.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Plant responses to salinity have been extensively studied over the last decades. Despite the vast accumulated knowledge, the ways Arabidopsis lateral roots (LR) cope with lethal salinity has not been fully resolved. Here we compared the primary root (PR) and the LR responses during events leading to lethal salinity (NaCl 200 mM) in Arabidopsis. We found that the PR and young LR
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In Vitro Induction of Pluripotency from Equine Fibroblasts in 20% or 5% Oxygen.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The cellular reprogramming into pluripotency is influenced by external and internal cellular factors, such as
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GV1001 interacts with androgen receptor to inhibit prostate cell proliferation in benign prostatic hyperplasia by regulating expression of molecules related to epithelial-mesenchymal transition.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Prostate cell proliferation, driven by testosterone, is a major characteristic of benign prostatic hyperplasia (BPH). GV1001, a human telomerase reverse transcriptase catalytic subunit, is an injectable formulation used as a cancer vaccine. It functions as a cell penetrating peptide to regulate cell proliferation. Here, we found that GV1001 effectively suppressed proliferation
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A New Protocol of Computer-Assisted Image Analysis Highlights the Presence of Hemocytes in the Regenerating Cephalic Tentacles of Adult
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
In humans, injuries and diseases can result in irreversible tissue or organ loss. This well-known fact has prompted several basic studies on organisms capable of adult regeneration, such as amphibians, bony fish, and invertebrates. These studies have provided important biological information and helped to develop regenerative medicine therapies, but important gaps concerning th
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Targeting the Redox Balance Pathway Using Ascorbic Acid in
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Patients with mutations in the β-subunit of the succinate dehydrogenase (
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Novelty-like activation of locus coeruleus protects against deleterious human pretangle tau effects while stress-inducing activation worsens its effects.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The earliest abnormality associated with Alzheimer's disease (AD) is the presence of persistently phosphorylated pretangle tau in locus coeruleus (LC) neurons. LC neuron numbers and fiber density are positive predictors of cognition prior to death. Using an animal model of LC pretangle tau, we ask if LC activity patterns influence the sequelae of pretangle tau. We seeded LC neu
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Overexpression of Peroxiredoxin 3 in Cartilage Reduces the Severity of Age-Related Osteoarthritis But Not Surgically Induced Osteoarthritis in Mice.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The study objective was to determine whether overexpression of the mitochondrial antioxidant peroxidase, peroxiredoxin 3 (Prx3), reduces the severity of osteoarthritis (OA) in mice. Age-related OA (age 18 and 24 months) and OA induced by destabilization of the medial meniscus (DMM at age 6 months) were assessed in male mice that overexpress a human Prdx3 transgene encoding the
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Ethyl acetate produced by
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
In this study, an oomycete strain FQ01 of
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Rewiring glucose metabolism improves 5-FU efficacy in p53-deficient/KRAS
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Despite the fact that 5-fluorouracil (5-FU) is the backbone for chemotherapy in colorectal cancer (CRC), the response rates in patients is limited to 50%. The mechanisms underlying 5-FU toxicity are debated, limiting the development of strategies to improve its efficacy. How fundamental aspects of cancer, such as driver mutations and phenotypic heterogeneity, relate to the 5-FU
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Ginsenoside protopanaxadiol protects adult retinal pigment epithelial-19 cells from chloroquine by modulating autophagy and apoptosis.
Cell type: Epithelial | Technique: Confocal / fluorescence | Disease/area: Imaging Techniques & Methods
Chloroquine often causes serious eye and vision problems, which are mainly mediated by lysosomotropic alteration. In this study, we investigated whether the ginsenoside protopanaxadiol relieves chloroquine-induced retinopathy by restoring lysosomotropic abnormalities in human adult retinal pigment epithelial-19 cells. Cytotoxicity was assessed using a 3-(4,5-dimethylthiazol-2-y
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Bioactive Properties of Kakadu Plum-Blended Products.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Kakadu plum (
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Optogenetic activation of UCP1-dependent thermogenesis in brown adipocytes.
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Brown adipocytes are unique in that they expend energy and produce heat to maintain euthermia through expression of uncoupling protein-1 (UCP1). Given their propensity to stimulate weight loss and promote resistance to obesity, they are a compelling interventional target for obesity-related disorders. Here, we tested whether an optogenetic approach could be used to activate UCP
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Direct tests of cytochrome
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The mitochondrial electron transport chain (ETC) of
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Flexible triboelectric nanogenerators using transparent copper nanowire electrodes: energy harvesting, sensing human activities and material recognition.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Triboelectric nanogenerators (TENGs) have emerged as a promising green technology to efficiently harvest otherwise wasted mechanical energy from the environment and human activities. However, cost-effective and reliably performing TENGs require rational integration of triboelectric materials, spacers, and electrodes. The present work reports for the first time the use of oxydat
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Asymbiotic Spore Production of
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
This study examined the effects of myristate on an asymbiotic culture of
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The Anti-Inflammatory Effect of Low Molecular Weight Fucoidan from
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Brown seaweed is a rich source of fucoidan, which exhibits a variety of biological activities. The present study discloses the protective effect of low molecular weight fucoidan (FSSQ) isolated from an edible brown alga,
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Engineering the sialome of mammalian cells with sialic acid mimetics.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Mammalian glycans show a diversity in sialic acid capping, constituting the sialome. Sialic acids can be extensively modified chemically, yielding sialic acid mimetics (SAMs). Here, we present a protocol for detecting and quantifying incorporative SAMs using microscopy and flow cytometry, respectively. We detail steps for linking SAMS to proteins with western blotting. Lastly,
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Exploring the effect of utilising organic acid solutions in ultrasound-assisted extraction of pectin from apple pomace, and its potential for biomedical purposes.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Biomass resulting from food production represents valuable material to recover different biomolecules. In our study, we used apple pomace to obtain pectin, which is traditionally extracted using mineral acids. Our hypothesis consisted of carrying out extractions with organic acids, assisted by ultrasound, by varying processing parameters including time, temperature, and type of
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Cyclic strain of poly (methyl methacrylate) surfaces triggered the pathogenicity of Candida albicans.
Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Candida albicans is an opportunistic yeast and the primary etiological factor in oral candidiasis and denture stomatitis. The pathogenesis of C. albicans could be triggered by several variables, including environmental, nutritional, and biomaterial surface cues. Specifically, biomaterial interactions are driven by different surface properties, including wettability, stiffness,
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Alternative oxidase promotes high iron tolerance in
Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The yeast
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Identification of a morphogene required for tapered filament termini in filamentous cyanobacteria.
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Although the photosynthetic cyanobacteria are monophyletic, they exhibit substantial morphological diversity across species, and even within an individual species due to phenotypic plasticity in response to life cycles and environmental signals. This is particularly prominent among the multicellular filamentous cyanobacteria. One example of this is the appearance of tapering at
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In vivo ameliorative effects of vitamin E against hydralazine-induced lupus.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
In this study, we investigated the in vivo ameliorative effects of vitamin E in a hydralazine-induced lupus model, which closely resembles SLE in humans. We aim to shed light on its potential as a therapeutic agent for managing SLE. Forty BALB/c mice were used in this study. Hydralazine hydrochloride was orally administered in a concentration of 25 mg/kg to the five mice groups
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Sirtuin 6 activation rescues the age-related decline in DNA damage repair in primary human chondrocytes.
Cell type: Chondrocyte | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
While advanced age is widely recognized as the greatest risk factor for osteoarthritis (OA), the biological mechanisms behind this connection remain unclear. Previous work has demonstrated that chondrocytes from older cadaveric donors have elevated levels of DNA damage as compared to chondrocytes from younger donors. The purpose of this study was to determine whether a decline
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Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) deletion in myeloid cells augments cholestatic liver injury.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Ductular reactive (DR) cells exacerbate cholestatic liver injury and fibrosis. Herein, we posit that tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) emanates from recruited macrophages and restrains DR cell expansion, thereby limiting cholestatic liver injury. Wild type (WT), Trail
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Lithium downregulates phosphorylated acetyl‑CoA carboxylase 2 and attenuates mitochondrial fatty acid utilization and oxidative stress in cardiomyocytes.
Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Acetyl-CoA carboxylase 2 plays a crucial role in regulating mitochondrial fatty acid oxidation in cardiomyocytes. Lithium, a monovalent cation known for its cardioprotective potential, has been investigated for its influence on mitochondrial bioenergetics. The present study explored whether lithium modulated acetyl-CoA carboxylase 2 and mitochondrial fatty acid metabolism in ca
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A retrotransposon-derived DNA zip code internalizes myeloma cells through Clathrin-Rab5a-mediated endocytosis.
Cell type: Cancer cell | Technique: Confocal / fluorescence | Disease/area: Imaging Techniques & Methods
We have demonstrated that transposons derived from ctDNA can be transferred between cancer cells. The present research aimed to investigate the cellular uptake and intracellular trafficking of Multiple Myeloma-zip code (MM-ZC), a cell-specific zip code, in myeloma cell lines. We demonstrated that MM-ZC uptake by myeloma cells was concentration-, time- and cell-type-dependent. F
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PIK3R1 fusion drives chemoresistance in ovarian cancer by activating ERK1/2 and inducing rod and ring-like structures.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Gene fusions are common in high-grade serous ovarian cancer (HGSC). Such genetic lesions may promote tumorigenesis, but the pathogenic mechanisms are currently poorly understood. Here, we investigated the role of a PIK3R1-CCDC178 fusion identified from a patient with advanced HGSC. We show that the fusion induces HGSC cell migration by regulating ERK1/2 and increases resistance
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Eupatolide, isolated from
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Ubiquitination of RIPK1 plays an essential role in the recruitment of the IKK complex, an upstream component of pro-survival NF-κB. It also limits TNF-induced programmed cell death by inhibiting the spatial transition from TNFR1-associated complex-I to RIPK1-dependent death-inducing complex-II or necrosome. Thus, the targeted disruption of RIPK1 ubiquitination, which induces RI
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Disease phenotypic screening in neuron-glia cocultures identifies blockers of inflammatory neurodegeneration.
Cell type: Neuron | Technique: Fluorescence imaging, live-cell | Disease/area: Imaging Techniques & Methods
Neuropathology is often mediated by interactions between neurons and glia that cannot be modeled by monocultures. However, cocultures are difficult to use and analyze for high-content screening. Here, we perform compound screening using primary neuron-glia cultures to model inflammatory neurodegeneration, live-cell stains, and automated classification of neurons, astrocytes or
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Long-term imaging and spatio-temporal control of living cells using targeted light based on closed-loop feedback.
Cell type: Cell culture models | Technique: Fluorescence imaging, live-cell | Disease/area: Imaging Techniques & Methods
The ability to optically interact with cells on both an individual and collective level has applications from wound healing to cancer treatment. Building systems that can facilitate both localised light illumination and visualisation of cells can, however, be challenging and costly. This work takes the Dynamic Optical MicroEnvironment (DOME), an existing platform for the closed
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Microfluidic production, stability and loading of synthetic giant unilamellar vesicles.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
In advanced drug delivery, versatile liposomal formulations are commonly employed for safer and more accurate therapies. Here we report a method that allows a straightforward production of synthetic monodisperse (~ 100 μm) giant unilamellar vesicles (GUVs) using a microfluidic system. The stability analysis based on the microscopy imaging showed that at ambient conditions the p
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The type of DNA damage response after decitabine treatment depends on the level of DNMT activity.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Decitabine and azacytidine are considered as epigenetic drugs that induce DNA methyltransferase (DNMT)-DNA crosslinks, resulting in DNA hypomethylation and damage. Although they are already applied against myeloid cancers, important aspects of their mode of action remain unknown, highly limiting their clinical potential. Using a combinatorial approach, we reveal that the effica
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Single-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The development of precise RNA-editing tools is essential for the advancement of RNA therapeutics. CRISPR (clustered regularly interspaced short palindromic repeats) PspCas13b is a programmable RNA nuclease predicted to offer superior specificity because of its 30-nucleotide spacer sequence. However, its design principles and its on-target, off-target and collateral activities
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Bioprinting of Synthetic Cell-like Lipid Vesicles to Augment the Functionality of Tissues after Manufacturing.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Bioprinting is an automated bioassembly method that enables the formation of human tissue-like constructs to restore or replace damaged tissues. Regardless of the employed bioprinting method, cells undergo mechanical stress that can impact their survival and function postprinting. In this study, we investigate the use of a synthetic cell-like unit, giant unilamellar vesicles (G
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Cigarette Smoke Extract Induces MUC5AC Expression Through the ROS/ IP3R/Ca
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Chronic obstructive pulmonary disease (COPD) is caused by exposure to noxious external particles, air pollution, and the inhalation of cigarette smoke. Airway mucus hypersecretion particularly mucin5AC (MUC5AC), is a crucial pathological feature of COPD and is associated with its initiation and progression. In this study, we aimed to investigate the effects of cigarette smoke e
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Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis.
Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Microbial metabolic engineering provides a feasible approach to sustainably produce advanced biofuels and biochemicals from renewable feedstocks. Methanol is an ideal feedstock since it can be massively produced from CO
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Silmitasertib (CX-4945) Disrupts ERα/HSP90 Interaction and Drives Proteolysis through the Disruption of CK2β Function in Breast Cancer Cells.
Cell type: Cancer cell | Technique: Fluorescence imaging, live-cell | Disease/area: Imaging Techniques & Methods
Aberrant estrogen receptor (ERα) signaling mediates detrimental effects of tamoxifen including drug resistance and endometrial hyperplasia. ERα36, an alternative isoform of ERα, contributes to these effects. We have demonstrated that CK2 modulates ERα expression and function in breast cancer (BCa). Here, we assess if CX-4945 (CX), a clinical stage CK2 inhibitor, can disrupt ERα
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P-NADs:
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Targeted protein degradation (TPD) allows cells to maintain a functional proteome and to rapidly adapt to changing conditions. Methods that repurpose TPD for the deactivation of specific proteins have demonstrated significant potential in therapeutic and research applications. Most of these methods are based on proteolysis targeting chimaeras (PROTACs) which link the protein ta
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Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integrated
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The waste pollution problem caused by polyethylene terephthalate (PET) plastics poses a huge threat to the environment and human health. As plasticizers, Phthalate esters (PAEs) are widely used in PET production and become combined pollutants with PET. Synthetic biology make it possible to construct engineered cells for microbial degradation of combined pollutants of PET and PA
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Cell-Int: a cell-cell interaction assay to identify native membrane protein interactions.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Intercellular protein-protein interactions (PPIs) have pivotal roles in biological functions and diseases. Membrane proteins are therefore a major class of drug targets. However, studying such intercellular PPIs is challenging because of the properties of membrane proteins. Current methods commonly use purified or modified proteins that are not physiologically relevant and henc
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GluN3A and Excitatory Glycine Receptors in the Adult Hippocampus.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The GluN3A subunit of
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Fluoride Alters Gene Expression via Histone H3K27 Acetylation in Ameloblast-like LS8 Cells.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Excessive fluoride ingestion during tooth development can cause dental fluorosis. Previously, we reported that fluoride activates histone acetyltransferase (HAT) to acetylate p53, promoting fluoride toxicity in mouse ameloblast-like LS8 cells. However, the roles of HAT and histone acetylation status in fluoride-mediated gene expression remain unidentified. Here, we demonstrate
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EbsA is essential for both motility and biofilm formation in the filamentous cyanobacterium
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Many cyanobacteria, both unicellular and filamentous, exhibit surface motility driven by type IV pili (T4P). While the component parts of the T4P machinery described in other prokaryotes are largely conserved in cyanobacteria, there are also several T4P proteins that appear to be unique to this phylum. One recently discovered component is EbsA, which has been characterized in t
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Locus coeruleus vulnerability to tau hyperphosphorylation in a rat model.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Post-mortem investigations indicate that the locus coeruleus (LC) is the initial site of hyperphosphorylated pretangle tau, a precursor to neurofibrillary tangles (NFTs) found in Alzheimer's disease (AD). The presence of pretangle tau and NFTs correlates with AD progression and symptomatology. LC neuron integrity and quantity are linked to cognitive performance, with degenerati
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High-throughput expansion microscopy enables scalable super-resolution imaging.
Cell type: Cardiomyocyte | Technique: Confocal / fluorescence | Disease/area: Imaging Techniques & Methods
Expansion microscopy (ExM) enables nanoscale imaging using a standard confocal microscope through the physical, isotropic expansion of fixed immunolabeled specimens. ExM is widely employed to image proteins, nucleic acids, and lipid membranes in single cells; however, current methods limit the number of samples that can be processed simultaneously. We developed High-throughput
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Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The present study investigated the photoprotective effect of the ultrasonic-assisted ethanol extract (USHE) from
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Benzothiazole-quinoline based probe for simultaneous colorimetric detection of CN- and Cu2+ ions, with fluorescence sensing for Cu2+: Mechanistic insights and practical applications in environmental monitoring and cellular analysis.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The development and synthesis of notably targeted and colorimetric sensor based on an azomethine compound for the distinct recognition of Cu
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EV-Elute: A universal platform for the enrichment of functional surface marker-defined extracellular vesicle subpopulations.
Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Intercellular communication via extracellular vesicles (EVs) has been identified as a vital component of a steadily expanding number of physiological and pathological processes. To accommodate these roles, EVs have highly heterogeneous molecular compositions. Given that surface molecules on EVs determine their interactions with their environment, EV functionality likely differs
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Transcriptomic and lipidomic profiling reveals distinct bioactive lipid signatures in slow and fast muscles and highlights the role of resolvin-D2 in fiber type determination during myogenesis.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Skeletal muscles are predominantly composed of long, multinucleated muscle fibers, classified according to their metabolic and contractile phenotype. The determination of fiber types is influenced by various factors (e.g., innervation, hormones, physical demand). Our laboratory and others showed that resolvins, lipid mediators derived from omega-3 fatty acids, promote muscle re
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Microscopic changes in the multifidus muscle in people with low back pain associated with lumbar disc herniation.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Lumbar disc herniation (LDH) is a common degenerative condition causing low back pain (LBP) due to nerve compression. Previous studies show conflicting findings regarding the multifidus (MF) muscle's microscopic changes in LDH patients. So, this study aimed to compare the affected MF to the adjacent MF on the ipsilateral and contralateral sides in LDH patients and examined corr
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Protein hydrolysates from
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Insect protein hydrolysates (PH) are emerging as valuable compounds with biological activity. The aim of the present study was to assess the potential cytoprotective effects of PH from the Black Soldier Fly (BPH, in the range 0.1-0.5 mg/mL) against inflammatory conditions and oxidative stress in LPS-challenged L-929 cells. BPH was effective in inhibiting LPS-induced ROS and nit
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Optimization of chemical transfection in airway epithelial cell lines.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Chemical transfection is a widely employed technique in airway epithelium research, enabling the study of gene expression changes and effects. Additionally, it has been explored for its potential application in delivering gene therapies. Here, we characterize the transfection efficiency of EX-EGFP-Lv105, an EGFP-expressing plasmid into three cell lines commonly used to model th
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Carbonylation of hair proteins: A robust biomarker of molecular and structural oxidative damage in hair fibres.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The first objective of this study is to compare two molecular markers, cysteic acid and protein carbonylation, to track the level of chemical oxidation and photochemical (UV) oxidation of human hair. The second objective is to investigate how the protein carbonylation biomarker evolution is associated with physical characteristics of the fibre. The third objective is to underst
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Mice Models for Peripheral Denervation to Enhance Vascular Regeneration.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Sympathetic innervation plays a critical role in regulating vascular function, yet its influence on vascular regeneration and reinnervation following ischemic injury remains poorly understood. This study develops and validates murine models of localized sympathetic denervation using 6-hydroxydopamine (6-OHDA) to enable study of the sympathetic nervous system's impact on vascula
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Isolation and Functional Characterization of Endophytic Bacteria from Muscadine Grape Berries: A Microbial Treasure Trove.
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Muscadine grapes are renowned for their unique traits, natural disease resistance, and rich bioactive compounds. Despite extensive research on their phytochemical properties, microbial communities, particularly endophytic bacteria, remain largely unexplored. These bacteria play crucial roles in plant health, stress tolerance, and ecological interactions. This study represents t
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