EVOS M5000 in Imaging Techniques & Methods: Independent Studies โ Wave 3 Part 2
Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.
EVOS M5000IndependentWave 3
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Select autosomal dominant DFNA11 deafness variants activate Myo7A targeting in epithelial cells.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Myosin-7A (Myo7A) is a motor protein crucial for the organization and function of stereocilia, specialized actin-rich protrusions on the surface of inner ear hair cells that mediate hearing. Variants in Myo7A cause several forms of genetic hearing loss, including autosomal dominant DFNA11 deafness. Despite its importance, the structural elements that control Myo7A within cells
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Engineering Long-Releasing Hollow-like or Condensed Progesterone Hormone Microcrystals with Controlled Polymorphism.
Cell type: Biological samples | Technique: Brightfield + fluorescence | Disease/area: Imaging Techniques & Methods
Progesterone is an endogenous steroid hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. Progesterone crystallization techniques have previously reported. Among these techniques, solvent crystallization and different solvent:anti-solvent systems are considered. Herein, the selective development of either hollow-like or condensed p
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Paradoxical Maturity-Onset Diabetes of the Young Arising From Loss-of-Function Mutations in ATP-Sensitive Potassium Channels.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Pancreatic ฮฒ-cell ATP-sensitive K+ (KATP) channel closure underlies electrical excitability and insulin release, but loss or inhibition of KATP channels can lead to paradoxical crossover from hyperinsulinism plus hypoglycemia, to glucose intolerance or diabetes. We report genotype-phenotype information from a set of patients clinically diagnosed with maturity-onset diabetes of
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Engineered bone-targeting apoptotic vesicles as a minimally invasive nanotherapy for heterotopic ossification.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Heterotopic Ossification (HO), refers to pathological extra skeletal bone formation, and there are currently no reliable methods except surgery to reverse these unexpected calcified tissues. Apoptotic vesicles (ApoEVs) are membrane-bound vesicles released by apoptotic cells, which are involved in metabolism regulation and intercellular communication. Due to its superior trauma-
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Effects of heterozygous SMG1 mutations on nonsense-mediated mRNA decay in human pluripotent stem cell model.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Nonsense-mediated mRNA decay (NMD) eliminates transcripts containing premature termination codons, thereby preventing errors in protein synthesis. Serine/threonine-protein kinase SMG1 is a crucial kinase for NMD response, interacting with other regulatory proteins such as SMG8 and SMG9. We identified a de novo heterozygous variant in SMG1 p.Gln2398Glu (c.7192C>G) in a patient w
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The dual-coding gene SLC35A4 protects against oxidative stress.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Alternative proteins (AltProts) represent a newly recognized class of biologically active proteins encoded from alternative open reading frames (AltORFs) within already annotated genes. This study focuses on the SLC35A4 gene, which encodes both the reference protein SLC35A4 and the alternative protein AltSLC35A4. Using a combination of microscopy and biochemical analyses, we co
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Anemoside B4 targets RAGE to attenuate ferroptosis in sepsis-induced acute lung injury.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Anemoside B4 (AB4), a major bioactive saponin isolated from the roots of To explore the therapeutic mechanism of AB4 in SALI, an integrated approach was employed, combining network pharmacology, molecular dynamics simulation, surface plasmon resonance (SPR) assays, AB4 ameliorated CLP-induced lung injury in mice, as evidenced by reduced pathological damage, lower injury scores,
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A reversible eugenol derived colorimetric and fluorescence "turn-off" probe for Fe
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
A simple and robust colorimetric and fluorescent eugenol-based chemical sensor, namely, (E)-N'-(5-allyl-2-hydroxy-3-methoxybenzylidene)-2-hydroxybenzohydrazide (EABH) was synthesized and characterized using spectroscopic techniques such as, NMR (
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Quantitative Profiling of Nanoscopic Protein Aggregates Reveals Specific Fingerprint of TDP-43-Positive Assemblies in Motor Neuron Disease.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Abnormal aggregation of TAR DNA-binding protein 43 (TDP-43) is a pathological hallmark of motor neuron disease (MND), yet current methods for quantifying these aggregates in biological samples remain limited in sensitivity and resolution. Here, single-molecule fluorescence microscopy is applied to post-mortem brain extracts to quantitatively characterize aggregates containing T
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Postnatal alterations in skeletal muscle fiber composition of myostatin-knockout chickens.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
In a previous study, we identified a hypermuscular phenotype attributed to both muscle fiber hyperplasia and hypertrophy in myostatin (MSTN)-knockout (KO) chickens generated by clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9) technology. In mammals, MSTN deletion results in increased production of fast glycolytic fibers, accompanied b
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Synthesis of thiophene derived fluorescent sensor for Hg
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
A new, simple, and robust thiol-based Schiff base ligand, (E)-3-(((2-mercaptophenyl) imino) methyl) pyridin-2-ol (PMP) as a chemical sensor for Hg
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Streaming potential analysis of hair variation: Ethnicities and environmental exposures.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
This study examined the ethnic and longitudinal (proximal 'root' vs. distal 'tip') variations in hair properties across Caucasian, Chinese and Indian populations, focusing on electrokinetic behaviour, structural features and particle deposition. The aim was to use electrokinetic analysis to determine how ethnicity and weathering affect hair health and influence hair-product int
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Fluorescent marker prediction for non-invasive optical imaging in bovine satellite cells using deep learning.
Cell type: Cell culture models | Technique: Brightfield + fluorescence | Disease/area: Imaging Techniques & Methods
Assessing the quality of bovine satellite cells (BSCs) is vital for advancing tissue engineered muscle constructs with applications in sustainable protein research. In this study, we present a non-invasive deep learning approach for optical imaging that predicts fluorescent markers directly from brightfield microscopy images of BSC cultures. Using a convolutional neural network
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Targeted genome modification in protoplasts of a tea cultivar Kolkhida using RNA-guided Cas9 endonuclease.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Gene-editing tools enable precise, targeted genome modifications, providing new approach for the rapid and sustainable improvement of tea plant (
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A new family of bacterial actin-like proteins regulates cell morphology in a filamentous cyanobacterium.
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Actin proteins are common to all domains of life and exhibit ATP-dependent polymerization to form filaments. In bacteria, four families of bacterial actin-like proteins (BALPs) have been identified and characterized. These BALPs are involved in plasmid partitioning (ParM), cell division (FtsA), magnetosome positioning (MamK), and cell morphology (MreB). Here, we report the iden
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Synthesis of iron oxide quantum dots by non-thermal atmospheric pressure plasma electrolysis: influence of operating conditions and biomedical applications.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Iron oxide quantum dots (IOQDs) were synthesized in this work using a distinct non-thermal atmospheric pressure plasma (NTAPP) electrolysis technique. The impact of the operational environment on the synthesis process was thoroughly investigated. The physicochemical properties of the synthesized IOQDs were studied using various methods, including scanning electron microscopy (S
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Methanol biotransformation for the production of biodegradable plastic monomer L-lactate in yeast.
Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Methanol is an ideal feedstock for biomanufacturing, and production of the biodegradable plastic monomer lactate from methanol is a promising approach for mitigating white pollution. However, it is challenging to engineer microbes for lactate production from methanol because of strong competition between product synthesis and cell growth. Here, Ogataea polymorpha is rewired to
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Lycopene Protects Deoxynivalenol-Induced Intestinal Barrier Dysfunction and NLRP3 Inflammasome Activation by Targeting the ERK Pathway.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
In agricultural production, Deoxynivalenol (DON) generally exists and contaminates wheat, corn, and other grains, causing intestinal damage and immunotoxicity. Lycopene (LYC), an antioxidant, anti-inflammatory carotenoid, is mainly found in red fruits such as tomatoes and has been investigated for its great medicinal advantages. This study aimed to investigate the protective ef
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Evaluating Linker Architecture in RNA-Detecting Riboglow Probes and Effects on Fluorescence Turn-On.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Riboglow probes are small molecules where a synthetic fluorophore is connected to an RNA-binding moiety via a chemical linker. Upon binding a short RNA sequence, probe fluorescence intensity and lifetime increase. The fluorescence change is modulated by the architecture of the chemical linker. Here, we systematically interrogated the linker composition in a series of Riboglow p
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Prenatal Intraportal Delivery of Polymeric Nanoparticles to Fetal Rhesus Monkeys (
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Preliminary investigations focused on biodistribution of polymeric nanoparticles (NPs) shortly after ultrasound-guided delivery via the portal vein in early second-trimester fetal rhesus monkeys. Results demonstrated that poly(lactic-co-glycolic acid) (PLGA) NPs (
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Microplastic-Mediated Delivery of Di-butyl Phthalate Alters
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Microplastics harbor chemical additives and absorb pollutants from the environment. Microplastics pose a human health threat and have been found in nearly all human tissues. The toxicological pathways and physiological effects of microplastic-mediated chemical exposure following ingestion remain unknown. Here we use
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Reduced LOXL3 Expression Disrupts Microtubule Acetylation and Drives TP53-Dependent Cell Fate in Glioblastoma.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Glioblastoma (GBM) is the most aggressive primary brain tumor, marked by molecular heterogeneity and poor clinical prognosis. Lysyl oxidase-like 3 (LOXL3) is frequently upregulated in GBM, but its mechanistic contribution remains insufficiently defined. Here, we investigated the functional role of LOXL3 in GBM using CRISPR-Cas9-mediated LOXL3 knockdown in two genetically distin
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Glycosylated NS3/NS3A protein of bluetongue virus facilitates efficient viral egress via lipid raft anchoring.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Bluetongue virus (BTV) is an economically important arbovirus of ruminants worldwide. The nonstructural glycoprotein NS3/NS3A contains a uniquely conserved N-linked glycosylation site at Asn
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Simtuzumab Attenuates Loxl2-Mediated Extracellular Matrix Remodeling and Preserves Cardiac Function in
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Dilated cardiomyopathy caused by Here, we employed human induced pluripotent stem cells (hiPSCs) derived from a patient carrying a We demonstrated that Taken together, our findings underscore the crucial role of Loxl2 as a therapeutic target and suggest that its inhibition could be a promising strategy to preserve cardiac function in
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Probiotic supplementation prevents stress-impaired spatial learning and enhances the effects of environmental enrichment.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Probiotics are live microorganisms that offer health benefits, influencing the microbiota-gut-brain axis. Probiotics can improve cognitive functions, including learning and memory, by modulating the gut microbiota, reducing inflammation, and producing neuroactive substances. This study examined the effects of probiotic supplementation prior to chronic stress or enrichment (EE)
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Identification of two
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
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A novel non-invasive imaging technique for mapping microplastic accumulation and distribution in different organ systems of Indian major carp
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Microplastic (MP) pollution poses an increasing threat to the aquatic ecosystems, yet most existing detection methods remain largely destructive and spatially limited. Here, we present a novel, non-invasive imaging framework integrated with micro-computed tomography (ยต-CT) and fluorescence microscopy to investigate ingestion, accumulation and internal distribution of fluorescen
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Micro S plit : semantic unmixing of fluorescent microscopy data.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Fluorescence microscopy is constrained by optical limits, fluorophore chemistry and finite photon budgets, imposing trade-offs between imaging speed, resolution and phototoxicity. Here we introduce
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Investigating the Impact of Carboxylated Polystyrene Nanoplastics in the Liver Using Cell Lines and Precision-Cut Liver Slices.
Cell type: Hepatocyte | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
Increasing reports of plastic accumulation in human tissue have raised concerns about potential adverse health outcomes. Evidence of negative effects of nanoplastics is heterogeneous and provides limited insights into the underlying pathogenic toxicity mechanisms in humans. In the present study, carboxylate-modified fluorescently labelled polystyrene nanoparticles (PS NPs) were
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<i>In Vitro</i> Phase Separation Characterization of the <i>Arabidopsis thaliana</i> Glycine-Rich RNA-Binding Protein AtGRP2.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Imaging Techniques & Methods
The
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