EVOS M7000 in Cancer Research
Curated literature review of peer-reviewed cancer studies that used the EVOS M7000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
Cancer researchers use the EVOS M7000 Imaging System for brightfield and fluorescence assays across tumour cell lines, organoids, and co-culture models. The papers below show how the platform supports proliferation, migration, invasion, cytotoxicity, and reporter-based readouts in oncology workflows.
JQKD82 Inhibits HIV Infection of Macrophages Through Blocking Viral Entry and Enhancing Antiviral ISG Responses.
Cell type: stem cells, macrophages
Imaging technique: fluorescence microscopy
Disease area: cancer, infectious disease, virology, inflammation
JQKD82 is an epigenetic modulator that inhibits lysine-specific demethylase 5 (KDM5), a host factor implicated in HIV latency and cell survival. Although JQKD82 has been studied in latent HIV infection of T cell models, its effects on HIV infection in macrophages remain unclear. Here, we investigated the impact of JQKD82 on HIV infection of primary human monocyte-derived macrophages (MDMs). We observed that treatment of MDMs with non-cytotoxic concentrations of JQKD82 dose-dependently inhibited ...
Journal of medical virology โ 2026 โ PMID 42403244
Google Scholar | PubMed | DOI
Spatially decoding genotype-associated epigenetic landscapes in human lymphoma FFPE tissues via epi-Patho-DBiT.
Cell type: HeLa cells, stem cells, macrophages, neurons, endothelial cells, epithelial cells
Imaging technique: live-cell imaging, confocal microscopy, brightfield microscopy
Disease area: cancer
While formalin-fixed paraffin-embedded (FFPE) samples are invaluable for human non-Hodgkin B-cell lymphoma translational research, effective methods for spatial profiling of chromatin accessibility and histone modifications in these tissues remain limited. Here, we introduce epi-Patho-DBiT, a platform that combines reverse crosslinking of FFPE tissues with spatially resolved assays for transposase-accessible chromatin using sequencing (spatial-FFPE-ATAC) or cleavage under targets and tagmentatio...
Nature communications โ 2026 โ PMID 42067542
Google Scholar | PubMed | DOI
CD177 Deficiency Defines a Stable Subtype of Human Neutrophil Granulocytes with Tumor Promoting Activity.
Cell type: stem cells, macrophages, endothelial cells
Imaging technique: confocal microscopy, brightfield microscopy
Disease area: cancer, infectious disease, virology, bacterial infection, diabetes, cardiovascular disease, inflammation
The surface protein CD177 on human neutrophil granulocytes is linked to tissue infiltration. In most individuals, circulating neutrophils comprise distinct fractions of CD177-expressing (CD177+) and non-expressing (CD177-) cells, which differ functionally and influence disease outcomes. Currently, CD177 is considered a dynamic activation marker ultimately expressed by most neutrophils. Here, we show that, instead, CD177--neutrophils represent a stable subpopulation that never acquires CD177. CD1...
Advanced science (Weinheim, Baden-Wurttemberg, Germany) โ 2026 โ PMID 42325214
Google Scholar | PubMed | DOI
Imaging-Guided Live Single-Cell Lipid Profiling of Leader and Follower Cellsduring Collective Migration of Triple-Negative Breast Cancer Cells.
Cell type: macrophages
Imaging technique: time-lapse microscopy
Disease area: cancer, infectious disease, virology
Leader and follower cells drive collective cancer cell migration through distinct and interactive roles, but their specific metabolic signatures remain largely unknown. Here, we developed and applied a workflow that integrates time-lapse imaging, single-cell tracking, microsampling and direct infusion high-resolution mass spectrometry to profile the lipidomes of individually sampled leader and follower cells from highly migratory TNBC lines HCC1143 and HCC38. Approximately 120 metabolites were d...
Advanced science (Weinheim, Baden-Wurttemberg, Germany) โ 2026 โ PMID 42301727
Google Scholar | PubMed | DOI
Surfaceome Reprogramming of Stemsomes Promotes Lung Cancer Targeting via Potentiated Receptor-Ligand Interactions.
Cell type: A549 lung cancer cells, stem cells, fibroblasts, epithelial cells
Imaging technique: fluorescence microscopy, confocal microscopy
Disease area: cancer, virology, diabetes, inflammation
Non-small-cell lung cancer (NSCLC) mortality remains high because mutation-specific therapies target only small patient subsets and inevitably encounter drug resistance. In this regard, multipathway-assisted mesenchymal stem cell-derived nanovesicles (stemsomes) offer a promising universal platform for overcoming the unmet needs of lung cancer therapeutics, particularly for patients lacking identifiable oncogenic drivers. This study introduces a novel strategy in which dexamethasone, a compound ...
Advanced science (Weinheim, Baden-Wurttemberg, Germany) โ 2026 โ PMID 42263250
Google Scholar | PubMed | DOI
Bioorthogonal Fluorogenic Reporters for Noninvasive Imaging and Urinalysis of Immunotherapeutic Response in Renal Cell Carcinoma.
Cell type: cell lines
Imaging technique: confocal microscopy
Disease area: cancer, inflammation
Real-time monitoring of kidney-infiltrating cytotoxic T lymphocytes (CTLs) is crucial for evaluating immunotherapy in renal cell carcinoma (RCC). However, existing imaging probes often exhibit "always-on" signals and poor renal clearance, limiting their ability to detect renal immune responses. Herein, we report renal-clearable bio-orthogonal near-infrared (NIR) fluorogenic probes (BGRs) that specifically detect granzyme B (GzmB), a biomarker of CTL activation, for dynamic evaluation of RCC immu...
Advanced science (Weinheim, Baden-Wurttemberg, Germany) โ 2026 โ PMID 42028639
Google Scholar | PubMed | DOI
NS2 of respiratory syncytial virus causes G2/M accumulation in HeLa/Fucci(CA)2 cells.
Cell type: HeLa cells, A549 lung cancer cells, hepatocytes, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, infectious disease, virology, bacterial infection, fibrosis
The cell cycle is a fundamental mechanism that regulates eukaryotic cell proliferation. It comprises the G1, S, G2, and M phases and is tightly controlled by cyclin/cyclin-dependent kinase complexes. Many viruses manipulate the host cell cycle to create a favorable environment for replication. In particular, RNA viruses such as Hepatitis C virus and human immunodeficiency virus interfere with cell cycle progression. Human respiratory syncytial virus (RSV), an RNA virus that infects the respirato...
Scientific reports โ 2026 โ PMID 42082631
Google Scholar | PubMed | DOI
Effects of Common Food Additives Kappa-, Iota- and Lambda-Carrageenans on Intestinal Epithelial Cell Activation and Barrier Disruption.
Cell type: HeLa cells, fibroblasts, epithelial cells
Imaging technique: confocal microscopy
Disease area: cancer, infectious disease, virology, bacterial infection, diabetes, inflammation
BACKGROUND: The epithelial barrier theory has been proposed to link the onset of many chronic inflammatory diseases to the damaged epithelial barrier induced by numerous environmental factors, including food emulsifiers. This study aimed to investigate the effects of food emulsifiers kappa-, iota- and lambda-carrageenan on intestinal epithelial barrier integrity and the underlying mechanisms. METHODS: The cytotoxicity and apoptosis of carrageenans were determined using Hoechst/propidium iodide s...
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology โ 2026 โ PMID 41942402
Google Scholar | PubMed | DOI
The CD49b (ITGA2) collagen receptor excludes CD8+ T cell infiltration in pancreatic ductal adenocarcinoma.
Cell type: HeLa cells, HEK293 cells, fibroblasts, macrophages, endothelial cells, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, virology, bacterial infection, fibrosis
Pancreatic ductal adenocarcinoma (PDAC) features a dense desmoplastic stroma that promotes tumor progression and immune evasion. We examined how the collagen receptor CD49b (ITGA2) shapes anti-tumor immunity and stromal architecture in PDAC. CD49b was highly expressed in tumors compared with normal pancreas and peripheral blood mononuclear cells. CD49b loss impaired PDAC cell adhesion to collagen, delayed tumor growth, and increased CD8+ T cell infiltration; depletion of CD8+ T cells in vivo res...
iScience โ 2026 โ PMID 42389601
Google Scholar | PubMed | DOI
3D-printed dictamni-calcium silicate scaffolds modulate the osteoimmune microenvironment and enhance macrophage-derived exosomal miR-21 signaling in vascularized bone regeneration.
Cell type: stem cells, fibroblasts, macrophages, neurons, keratinocytes, endothelial cells
Imaging technique: confocal microscopy
Disease area: cancer, infectious disease, bacterial infection, inflammation
Reconstruction of critical-sized bone defects remains a formidable clinical challenge due to a persistent, localized, pro-inflammatory microenvironment that disrupts the coordinated process of osteogenic-angiogenic coupling. To address this, we engineered 3D-printed calcium silicate scaffolds containing Dictamni Radicis Cortex to modulate the osteoimmune niche. In vitro models demonstrated that Dic5 scaffolds effectively attenuated the pro-inflammatory state, promoting the transition of macropha...
Journal of nanobiotechnology โ 2026 โ PMID 42098777
Google Scholar | PubMed | DOI
S100A9 modulates USP7-mediated stabilization of NCOA4 to promote ferroptosis in sepsis-associated acute lung injury.
Cell type: HeLa cells, HEK293 cells, macrophages, endothelial cells, epithelial cells
Imaging technique: fluorescence microscopy, live-cell imaging, confocal microscopy
Disease area: cancer, infectious disease, bacterial infection, inflammation
Sepsis-associated acute lung injury (SALI) is driven by dysregulated macrophage activation; however, mechanisms linking innate immune signaling to cell death remain elusive. Emerging evidence implicates ferroptosis, fueled by NCOA4-mediated ferritinophagy, as a critical executioner of macrophage death. Yet, post-translational mechanisms dictating NCOA4 stability and preventing its premature degradation during sepsis are poorly understood. Specifically, how damage-associated molecular patterns (D...
Redox biology โ 2026 โ PMID 42330601
Google Scholar | PubMed | DOI
Pomegranate peel extract alleviates diabetic retinopathy by suppressing the PI3K/AKT/HIF-1ฮฑ/VEGF pathway and gut microbiota modulation.
Cell type: HeLa cells, HepG2 hepatoma cells, macrophages, endothelial cells, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, Alzheimer's disease, virology, bacterial infection, diabetes, cardiovascular disease, inflammation
INTRODUCTION: Diabetic retinopathy (DR) is a severe microvascular complication of diabetes mellitus. Pomegranate peel extract (PPE) has shown potential in mitigating various diabetic complications, yet its role in DR remains unexplored. OBJECTIVE: To investigate the beneficial effects and underlying action mechanisms of PPE in managing DR. METHODS: PPE was extracted using 50ย % ethanol. The effects and underlying mechanisms of PPE on DR were evaluated in streptozotocin (STZ)-induced DR rats and h...
Journal of advanced research โ 2026 โ PMID 41201496
Google Scholar | PubMed | DOI
Patient-derived tumor organoids for personalized cancer immunotherapy: An immunopeptidome-to-validation approach in RCC and BC.
Cell type: stem cells, fibroblasts, macrophages, hepatocytes, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, virology, inflammation, drug screening
Immunotherapy has revolutionized cancer treatment, yet clinical success remains limited, with only a fraction of patients responding. Tumor heterogeneity and patient-specificity hinder response prediction, emphasizing the need for human-based models that accurately reproduce tumor-immune interactions. Conventional preclinical platforms, such as murine models, lack the human-specific HLA-TCR complexity, limiting their ability to accurately evaluate immunotherapy responses. To address this, we est...
Molecular therapy. Oncology โ 2026 โ PMID 42375394
Google Scholar | PubMed | DOI
Formulation Characteristics of Solid-Dispersible Self-Emulsifying Drug Delivery Systems for Dual Drug Delivery.
Cell type: cell lines
Imaging technique: fluorescence microscopy, brightfield microscopy, Z-stack imaging
Disease area: cancer
Background: Oral delivery of chemotherapeutic agents remains challenging due to gastrointestinal degradation, poor intestinal permeability, and extensive first-pass metabolism, which collectively limit bioavailability. Lipid-based drug delivery systems offer a promising strategy to overcome these barriers. This study aimed to develop a freeze-dried, solid-dispersible self-emulsifying drug delivery system (SEDDS) using a water-in-oil-in-water (w/o/w) double emulsion approach for the co-encapsulat...
Pharmaceutics โ 2026 โ PMID 42357254
Google Scholar | PubMed | DOI
Targeting Bruton tyrosine kinase with acalabrutinib attenuates murine sclerodermatous chronic graft versus host disease.
Cell type: stem cells, fibroblasts, macrophages, keratinocytes, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, infectious disease, fibrosis, inflammation
BACKGROUND: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is considered the only curative option for certain malignant hematopoietic disorders, yet its benefits are limited by the development of graft-versus-host disease (GVHD). Chronic GVHD (cGVHD) is often characterized by chronic inflammation and fibrosis, propagated by alloreactive donor B- and T-cell involvement. Bruton tyrosine kinase (BTK) is the downstream effector in the B-cell receptor signaling pathway and further has...
Frontiers in immunology โ 2026 โ PMID 42367798
Google Scholar | PubMed | DOI
Preclinical Drug-Response Profiling Identifies BMI1 Inhibition as a Therapeutic Option for Hepatoblastoma.
Cell type: HEK293 cells, MCF-7 breast cancer cells, HepG2 hepatoma cells, stem cells, fibroblasts, keratinocytes, epithelial cells
Imaging technique: brightfield microscopy
Disease area: cancer, drug screening
Hepatoblastoma (HB), the most common pediatric liver cancer, exhibits marked variability in therapeutic response despite minimal genetic heterogeneity, implicating epigenetic regulation as a key driver of tumor behavior. Among these, polycomb repressor complexes (PRC) remain poorly explored as therapeutic targets. Integrative analysis of samples from patients with HB and public datasets identified BMI1, a core component of PRC1, as significantly upregulated, with high expression strongly associa...
International journal of molecular sciences โ 2026 โ PMID 42352961
Google Scholar | PubMed | DOI
Small-Molecule Targeting of VDAC Disrupts Mitochondrial Bioenergetics and Suppresses Melanoma Cell Survival and Migration.
Cell type: cell lines
Imaging technique: fluorescence microscopy
Disease area: cancer
Melanoma is a highly aggressive and metabolically adaptable cancer that often resists conventional therapies. Targeting core bioenergetic pathways may, therefore, represent an effective strategy to improve therapeutic responses, particularly in tumors dependent on mitochondrial function. SC18 is an imidazolidine-2,4-dione compound that binds the NADH-binding pocket of voltage-dependent anion channels (VDACs), inducing mitochondrial dysfunction. VDAC expression is increased in melanoma and strong...
Cells โ 2026 โ PMID 42346093
Google Scholar | PubMed | DOI
RNA helicase DDX6 governs ASC speck formation in P-bodies and the transition to stress granules via phase separation during inflammasome activation.
Cell type: HEK293 cells, stem cells, macrophages, epithelial cells
Imaging technique: live-cell imaging, time-lapse microscopy, confocal microscopy
Disease area: cancer, infectious disease, virology, bacterial infection, COVID-19, cardiovascular disease, inflammation
The recruitment and condensation of apoptosis-associated speck-like protein containing a CARD (ASC) are critical for ASC speck formation and inflammasome activation. However, how this process occurs efficiently in vivo remains unclear. Here, we identified the RNA helicase DDX6 as an ASC-interacting protein through immunoprecipitationโmass spectrometry (IPโMS) analysis. DDX6 promotes the activation of both NLRP3 and AIM2 inflammasomes by facilitating the recruitment of ASC to these receptors thro...
Cell discovery โ 2026 โ PMID 42342646
Google Scholar | PubMed | DOI
Genetic disruption of Capns1 impairs metastatic phenotypes in murine mammary carcinoma cells.
Cell type: HEK293 cells
Imaging technique: fluorescence microscopy
Disease area: cancer, virology, bacterial infection
Calpains are a family of 15 calcium-activated cysteine proteases that have emerged as potential antimetastatic targets in breast cancer. Calpain-1 and calpain-2 are ubiquitously expressed heterodimers composed of unique catalytic subunits (encoded by Capn1 and Capn2 , respectively) and a common regulatory subunit (encoded by Capns1 ). Genetic disruption of Capns1 abolishes calpain-1 and calpain-2 activity. Using CRISPR-Cas9 mediated Capns1 knockout, we validated calpains-1/2 as promising therape...
microPublication biology โ 2026 โ PMID 42359282
Google Scholar | PubMed | DOI
Feasibility of PIK3CA Mutation Detection via Microfluidic Isolation of Disseminated Tumor Cells from Bone Marrow in Breast Cancer Patients.
Cell type: MCF-7 breast cancer cells, stem cells
Imaging technique: fluorescence microscopy
Disease area: cancer
Disseminated tumor cells originating from the bone marrow serve as precursors of distant metastasis and are associated with increased mortality in breast cancer. With the emergence of targeted therapies for breast cancer, accurate procedures for enrichment of disseminated tumor cells are crucial to investigate the molecular heterogeneity between disseminated tumor cells and primary tumors. This study evaluated the feasibility of PIK3CA mutation analysis in disseminated tumor cells enriched using...
Geburtshilfe und Frauenheilkunde โ 2026 โ PMID 42345027
Google Scholar | PubMed | DOI
IGF2BP2-driven serine metabolism promotes the progression of thyroid carcinoma via m6A-PHGDH.
Cell type: stem cells, macrophages
Imaging technique: confocal microscopy
Disease area: cancer, infectious disease, virology
BACKGROUND: Thyroid cancer (TC) is one of the most common malignant tumors of the endocrine system, and patients with advanced and metastatic TC have a poor prognosis. Serine metabolism is an important component of tumor cell metabolic reprogramming. However, the molecular mechanism underlying the upregulation of serine metabolism in TC remains poorly understood. METHODS: Immunohistochemistry was performed to observe the expression of IGF2BP2 and its target molecules in TC and adjacent tissues. ...
Journal of translational medicine โ 2026 โ PMID 42174614
Google Scholar | PubMed | DOI
Fasting requires Peroxiredoxin 2 and Peroxiredoxin 6 to coordinate redox dependent mitochondrial and lipid remodelling in Caenorhabditis elegans.
Cell type: neurons
Imaging technique: fluorescence microscopy
Disease area: cancer, infectious disease, bacterial infection, diabetes
Fasting induces conserved metabolic and redox adaptations that promote stress resistance and longevity. However, the molecular mechanisms linking transient redox changes and altered metabolism to downstream signalling events remain incompletely understood. Using Caenorhabditis elegans, the roles of peroxiredoxins in coordinating redox-dependent responses to fasting and refeeding were determined. A 4-hr fasting protocol over 5 days extended lifespan, improved late-life physiological activity, red...
Redox biology โ 2026 โ PMID 42275699
Google Scholar | PubMed | DOI
The pentose phosphate pathway contributes to excess lactate production in radiation-induced fibroblast to myofibroblast transdifferentiation.
Cell type: fibroblasts, macrophages, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, infectious disease, virology, diabetes, fibrosis
Thoracic radiation is an effective mainstay treatment for lung cancer; however, patients risk developing an adverse side effect known as radiation-induced lung injury (RILI). RILI is dose-limiting, can be permanent, and may threaten normal lung function, but the underlying mechanism is not well characterized. RILI can include both inflammatory (radiation pneumonitis) and fibrotic (radiation-induced lung fibrosis) pathologies. Myofibroblasts are the main effector cells of fibrosis, and we and oth...
The Journal of biological chemistry โ 2026 โ PMID 42134543
Google Scholar | PubMed | DOI
Target sequence-conditioned design of peptide binders using masked language modeling.
Cell type: HeLa cells, HEK293 cells, fibroblasts
Imaging technique: fluorescence microscopy
Disease area: cancer, infectious disease, virology, COVID-19
The computational design of protein-based binders presents unique opportunities to access 'undruggable' targets, but effective binder design often relies on stable three-dimensional structures or structure-influenced latent spaces. Here we introduce PepMLM, a target sequence-conditioned designer of de novo linear peptide binders. Using a masking strategy that positions cognate peptide sequences at the C terminus of target protein sequences, PepMLM finetunes the ESM-2 protein language model to fu...
Nature biotechnology โ 2026 โ PMID 40804173
Google Scholar | PubMed | DOI
Comprehensive genomics and functional analyses identify SMARCAL1 as a key oncogenic regulator in lung adenocarcinoma.
Cell type: A549 lung cancer cells, macrophages, neurons
Imaging technique: fluorescence microscopy
Disease area: cancer, diabetes
BACKGROUND: SMARCAL1, a member of the SNF2-family DNA translocases, plays a crucial role in maintaining genomic stability, and its dysregulation may contribute to tumorigenesis. This study investigated the potential of SMARCAL1 as a biomarker in lung adenocarcinoma (LUAD). METHODS: Transcriptomic and clinical data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets were analyzed to assess SMARCAL1 expression patterns and biological functions. Quantitative polymerase ch...
Translational cancer research โ 2026 โ PMID 42305511
Google Scholar | PubMed | DOI
Direct coupling and protective activation of DRP1 by the DNA-PKcs inhibitor KU-57788 synergizes with ferroptosis in anaplastic thyroid cancer cells.
Cell type: fibroblasts, macrophages
Imaging technique: confocal microscopy
Disease area: cancer, diabetes
Anaplastic thyroid carcinoma (ATC) is one of the most aggressive and lethal malignancies, with limited treatment options and poor clinical outcomes. KU-57788, a selective inhibitor of DNA-dependent protein kinase catalytic subunit (DNA-PKcs), has shown promise in cancer therapy when combined with radiotherapy and chemotherapy. However, its therapeutic potential and underlying mechanisms in ATC remain unclear. In this study, we demonstrate that KU-57788 exerts potent anti-ATC activity both in vit...
Cell death & disease โ 2026 โ PMID 42049705
Google Scholar | PubMed | DOI
CD8 immunoPET imaging to stratify response and guide combination immunotherapy and radiation in triple negative breast cancer.
Cell type: macrophages
Imaging technique: fluorescence microscopy
Disease area: cancer, virology
PURPOSE: PET imaging targeting immune cells can be used to dynamically monitor intratumoral immune modulation in tissues. Radiation therapy is known to alter the tumor immune microenvironment; therefore, this study demonstrates how CD8 immunoPET imaging can optimize combination immunotherapy and radiation therapy by stratifying tumors who could derive the greatest benefit from immunotherapy following radiation therapy. EXPERIMENTAL DESIGN: A radiation resistant triple negative breast cancer cell...
Breast cancer research : BCR โ 2026 โ PMID 42035192
Google Scholar | PubMed | DOI
Senescent-associated ฮฒ-galactosidase accumulation does not correlate with lifespan in different strains of Nothobranchius furzeri but does respond to intermittent fasting to extend lifespan.
Cell type: stem cells
Imaging technique: fluorescence microscopy
Disease area: cancer
Senescent cell (SC) accumulation theoretically causes aging-related tissue malfunction. We examined SC burden in short-lived and long-lived Nothobranchius furzeri fish strains, and fasting's effects on SCs. Despite different lifespans, SC accumulation rates didn't correspond between strains. The long-lived strain showed sex-based lifespan differences without SC burden differences. A 14-day fast reduced SC burden in short-lived fish, but this effect was temporary. Fasting extended median lifespan...
microPublication biology โ 2026 โ PMID 42294401
Google Scholar | PubMed | DOI
Novel African American Colorectal Cancer MSH3 Variants Associate With Major Genomic Instability.
Cell type: cell lines
Imaging technique: fluorescence microscopy
Disease area: cancer, bacterial infection, inflammation
UNLABELLED: Pathogenic variants of MSH3 can increase mutational load within colorectal cells, which may drive initiation and progression of colorectal cancer (CRC). We identified several variants within MSH3 among CRCs from African Americans (AA). To predict and assess the functional significance of these MSH3 variants, we employed a combination of in silico analyses and in vitro functionality assays. Our objective was to elucidate the correlation between computational predictions and functional...
Human mutation โ 2026 โ PMID 42253507
Google Scholar | PubMed | DOI
CpG 1018 augments mRNA vaccine-induced anti-tumor immunity by potentiating CD8+ T cell responses.
Cell type: stem cells, macrophages
Imaging technique: fluorescence microscopy
Disease area: cancer, virology, bacterial infection, COVID-19, inflammation
Personalized neoantigen mRNA vaccines showed good efficacy in treating metastatic melanoma, pancreatic cancer, and breast cancer in early phase clinical trials. Strategies are needed to further enhance neoantigen mRNA vaccine-induced anti-tumor immunity. This study explored conventional adjuvant co-administration to enhance mRNA vaccine-induced anti-tumor immunity. We found a clinically used CpG 1018 adjuvant was highly effective to enhance ovalbumin (OVA) and neoantigen mRNA-induced T cell resp...
Molecular therapy. Oncology โ 2026 โ PMID 42256206
Google Scholar | PubMed | DOI
Effects of SRWยฎ Stem on the functional properties, oxidative stress and inflammation of human umbilical cord mesenchymal stem cells.
Cell type: stem cells, fibroblasts, macrophages
Imaging technique: fluorescence microscopy
Disease area: cancer, Alzheimer's disease, Parkinson's disease, diabetes, cardiovascular disease, inflammation
BACKGROUND: Global population ageing has heightened the demand for anti-ageing nutritional interventions. Science Research Wellness (SRWยฎ) Stem was designed by SRWยฎ to address this demand with ingredients that improve stem cell function, which is central to protection against age-related decline. These ingredients include mitoquinol, sea buckthorn extract, fucoidan, oleuropein, and vitamin D3. OBJECTIVES: We compared the effects of SRWยฎ Stem on the functions of human umbilical cord mesenchymal s...
Food & nutrition research โ 2026 โ PMID 42232740
Google Scholar | PubMed | DOI
Polyamine homeostasis in Caenorhabditis elegans relies primarily on transport.
Cell type: stem cells, fibroblasts, neurons
Imaging technique: confocal microscopy, brightfield microscopy, Z-stack imaging
Disease area: cancer, Parkinson's disease, virology, bacterial infection
Polyamines are essential metabolites present in all cells, but their regulation in vivo remains poorly understood. Little is known about whether tissues maintain distinct polyamine setpoints, how these setpoints are established, or whether such differences underlie selective vulnerability in disease. Here, we applied single-cell polyamine measurements in living Caenorhabditis elegans to map tissue polyamine levels and their regulatory dependencies. Three principles emerge: (1) across differentia...
Cell reports โ 2026 โ PMID 42001420
Google Scholar | PubMed | DOI
Multimodal spatial-omics reveal co-evolution of alveolar progenitors and proinflammatory niches in progression of lung precursor lesions.
Cell type: stem cells, fibroblasts, macrophages, endothelial cells, epithelial cells
Imaging technique: confocal microscopy
Disease area: cancer, cardiovascular disease, fibrosis, inflammation
The co-evolution of different cell subsets in the progression of precursor lesions to lung adenocarcinoma (LUAD) is incompletely understood. We generated spatial transcriptomic maps of 56 human precursor lesions and LUADs from 25 patients and of an independent cohort of 36 lesions from 19 patients, analyzing a total of 486,519 spots and 5.4 million cells. We identify region-specific programs that distinguish precursors from LUADs. Spatially resolved clonal architectures reveal patient-specific h...
Cancer cell โ 2026 โ PMID 41202811
Google Scholar | PubMed | DOI
Importin ฮฑ regulates ciliogenesis and cilia length with implications for Xenopus nephrogenesis.
Cell type: stem cells, epithelial cells
Imaging technique: confocal microscopy
Disease area: cancer, virology
Cilia are microtubule-based organelles that are essential for a wide range of biological processes ranging from facilitating fluid flow to transducing developmental and growth signals. Defects in cilia structure or function can lead to ciliopathies. Ciliogenesis and cilia length regulation depend on protein transport to the ciliary base, but the underlying molecular mechanisms remain unclear. Here, we identify that the nuclear adapter protein importin ฮฑ-1 (encoded by KPNA2 in humans; hereafter i...
Journal of cell science โ 2025 โ PMID 41017282
Google Scholar | PubMed | DOI
SiRNA-mediated knockdown of TOP2B protects hiPSC-derived cardiomyocytes from doxorubicin-induced toxicity.
Cell type: HeLa cells, stem cells, iPSCs, fibroblasts, neurons
Imaging technique: confocal microscopy, brightfield microscopy
Disease area: cancer, cardiovascular disease, inflammation
AIMS: Doxorubicin (Dox) is a potent chemotherapeutic agent, but its use is limited by cardiotoxicity, primarily due to the disruption of Topoisomerase-2 beta (TOP2B) activity. Dexrazoxane (Dex), an FDA-approved cardioprotective drug, alleviates Dox-induced toxicity but lacks heart-specific targeting. This study investigates siRNA-mediated TOP2B knockdown as a more targeted strategy to protect cardiomyocytes from Dox-induced damage. MATERIALS AND METHODS: Human induced pluripotent stem cell-deriv...
Life sciences โ 2025 โ PMID 40158615
Google Scholar | PubMed | DOI
Mechanistic models position ceritinib as a nuclear integrity disrupting therapy in pediatric liver tumors.
Cell type: HepG2 hepatoma cells, fibroblasts, keratinocytes, epithelial cells
Imaging technique: fluorescence microscopy, brightfield microscopy
Disease area: cancer, drug screening
BACKGROUND: Pediatric liver tumors with high-risk features pose therapeutic challenges, necessitating the development of more targeted and effective treatment strategies. Computational modeling of virtual patients and in silico drug response simulations, based on properly trained mechanistic models, is a powerful strategy to predict new treatment options. We aimed to leverage patient-specific mechanistic cell models to identify therapeutic alternatives for pediatric patients with high-risk liver...
Journal of experimental & clinical cancer research : CR โ 2025 โ PMID 40968384
Google Scholar | PubMed | DOI