EVOS M7000 in Cell Biology Research: Cell Models, Techniques, and Disease Areas
Curated literature review of peer-reviewed studies that used the EVOS M7000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
The EVOS M7000 Imaging System is used across a wide range of cell biology workflows, from spheroid killing assays and stem-cell differentiation to infection biology and materials science. The papers below show how researchers combine brightfield and fluorescence imaging on the M7000 to quantify morphology, viability, and reporter expression.
IRX3 depletion promotes early cardiac commitment of hiPSC-Derived Cardiomyocytes.
Cell type: stem cells, iPSCs, fibroblasts
Imaging technique: fluorescence microscopy, high-content imaging, confocal microscopy
Disease area: cancer, virology, COVID-19, cardiovascular disease
Generating mature human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) remains a major obstacle to accurate disease modeling and cardiac repair. As the transcription factor Irx3 is a key determinant of ventricular conduction system fate in mice, we hypothesized that suppressing IRX3 expression accelerates human working cardiomyocyte differentiation. Here, we demonstrate that depleting IRX3 enhances hiPSC-CM differentiation. IRX3-knockout (KO) hiPSCs generated a greater number o...
PloS one β 2026 β PMID 42302071
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Recapitulating apicobasal tissue polarity in extracellular matrix-incorporated airway organoids.
Cell type: stem cells, iPSCs, fibroblasts, epithelial cells
Imaging technique: confocal microscopy, brightfield microscopy, Z-stack imaging
Disease area: cancer, infectious disease, virology, COVID-19, fibrosis, inflammation
The airway epithelium is a dynamic barrier that interfaces with the external environment and internal matrix along its apicobasal axis. To recapitulate this spatial organization and associated cell-ECM interactions in an organoid format, we present the decellularized extracellular matrix-incorporated Apical-out Airway Organoid (dECM-AoAO), which integrates basolateral matrix cues through the incorporation of human lung dECM microparticles (dECM-MPs), while maintaining direct apical exposure to t...
Biomaterials β 2026 β PMID 41722465
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Regulation of tension-dependent localization of LATS1 and LATS2 to adherens junctions.
Cell type: HEK293 cells, stem cells, epithelial cells
Imaging technique: live-cell imaging
Disease area: cancer, virology
The LIM domain protein LIMD1 is a critical regulator of the Hippo signaling pathway, acting to sequester the kinases LATS1/2 to adherens junctions (AJs) in response to mechanical strain. Here, we identify the molecular basis for LIMD1 binding and recruitment of LATS1/2 to AJs. We show that while the LIM domains of LIMD1 are sufficient for AJ localization and binding to LATS1/2, recruitment of LATS1 to AJ requires both the intrinsically disordered region (IDR) in the N-terminus as well as the LIM...
PloS one β 2026 β PMID 41628219
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Surface colonization of Aureobasidium pullulans: a multimodal microscopy study toward living coating development.
Cell type: endothelial cells
Imaging technique: fluorescence microscopy, confocal microscopy
Disease area: bacterial infection
Recent advances in engineered living materials (ELMs), which integrate living cells into functional structural and protective systems, have accelerated interest in understanding microbial surface interactions at fundamental scales. In this context, Aureobasidium pullulans, a polymorphic and polyextremotolerant black yeast fungus, has emerged as a promising candidate for diverse biotechnological applications, including engineered living coatings. However, its microscale surface colonization dynam...
Scientific reports β 2026 β PMID 41951758
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Collagen hybridizing peptide imaging and delivery of therapeutic antibody in rheumatoid arthritis models.
Cell type: fibroblasts, macrophages, endothelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, infectious disease, virology, bacterial infection, fibrosis, inflammation
The use of immunosuppressive therapies for rheumatoid arthritis (RA) is associated with substantial adverse effects. In addition, existing diagnostic reagents are limited in their ability to detect local joint damage. Here, we address both these needs using a collagen-hybridizing peptide (CHP) that selectively binds denatured collagen present in inflamed joints in RA models. Light-sheet scanning and immunohistology of the inflamed ankles in RA mice pre-injected with fluorescently-labeled-CHP rev...
Nature communications β 2026 β PMID 42010292
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VSIG10L is a major determinant of esophageal homeostasis and inherited predisposition to Barrett's esophagus.
Cell type: HeLa cells, stem cells, fibroblasts, keratinocytes, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, inflammation
The molecular underpinnings contributing to the onset of Barrett's esophagus (BE) remain elusive. By studying familial clusters of the disease, here we identify a significant association between genetic variants in the V-set and Immunoglobulin Domain Containing 10 Like (VSIG10L) gene and BE predisposition. Using mammalian tissues and patient-derived organoids, we show VSIG10L is selectively expressed in the suprabasal squamous cells of the esophageal mucosa and is essential for epithelial matura...
Nature communications β 2026 β PMID 41611715
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Polydopamine-Coated Surfaces Promote Adhesion, Migration, Proliferation, Chemoresistance, Stemness, and Epithelial-Mesenchymal Transition of Human Prostate Cancer Cell Lines In Vitro via Integrin Ξ±2Ξ²1-FAK-JNK Signaling.
Cell type: stem cells, fibroblasts, macrophages, keratinocytes, endothelial cells, epithelial cells
Imaging technique: confocal microscopy
Disease area: cancer, inflammation, drug screening
Polydopamine (PDA) surface coatings are widely used in biomedical engineering to enhance cell-substrate interactions; however, their effects on cancer-cell behavior remain unclear. In this study, we investigated how PDA-coated two-dimensional (2D) culture surfaces influence oncogenic traits of human prostate cancer (PC) cells in vitro. Using LNCaP, DU145, and PC3 cell lines, we found that PDA-coated substrates markedly increased the adhesion, migration, invasion, proliferation, and colony format...
International journal of molecular sciences β 2026 β PMID 41596307
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Acacetin Attenuates Lysophosphatidylcholine-Induced Vascular Smooth Muscle Cell Injury via Sirt1-Nrf2/p62 Signaling Axis.
Cell type: A549 lung cancer cells, fibroblasts, macrophages, endothelial cells
Imaging technique: time-lapse microscopy, confocal microscopy
Disease area: cancer, diabetes, cardiovascular disease, inflammation
Background: Acacetin, a naturally occurring flavone present in various plants, is known as a promising drug candidate for cardiovascular disorders. Our previous study demonstrated that acacetin ameliorates atherosclerosis through endothelial cell protection; however, its pharmacological effects on vascular smooth muscle cells (VSMCs) remain unexplored. This study investigates the therapeutic potential of acacetin against lysophosphatidylcholine (LysoPC)-induced VSMC injury and elucidates the und...
Biomedicines β 2026 β PMID 41595728
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Transcranial microtesla magnetic fields suppress neuroinflammation and neuronal oxidative stress burden.
Cell type: SH-SY5Y neuroblastoma cells, iPSCs, macrophages, neurons, endothelial cells
Imaging technique: high-content imaging, brightfield microscopy, Z-stack imaging
Disease area: cancer, Alzheimer's disease, Parkinson's disease, virology, COVID-19, diabetes, inflammation
Neuroinflammation is a major driver of neurodegenerative and psychiatric disease, yet current therapies have limited brain penetration and efficacy. We investigated microtesla magnetic therapy (MMT), a brief transcranial exposure to time-varied electromagnetic fields (TV-EMFs), as a noninvasive approach to modulate neuroimmune inflammation. In human peripheral blood mononuclear cells, MMT suppressed lipopolysaccharide (LPS)-induced TNFΞ± and IL-1Ξ² release and reduced NF-ΞΊB activation in monocyte ...
iScience β 2026 β PMID 41550771
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Apical-out airway organoids as a pathophysiological model to study macrophage-mediated epithelial responses to microplastics.
Cell type: stem cells, macrophages, epithelial cells
Imaging technique: fluorescence microscopy, confocal microscopy, Z-stack imaging
Disease area: cancer, infectious disease, virology, bacterial infection, COVID-19, cardiovascular disease, fibrosis, inflammation, drug screening
Respiratory exposure to airborne microplastics is increasingly implicated in airway inflammation, but human-relevant in vitro systems that separate direct epithelial particle contact from immune-cell-mediated paracrine effects remain limited. Here, we generated human airway basal stem cells (hABSCs)-derived apical-out airway organoids (AOAOs) with outward-facing ciliated surfaces and used them to evaluate operationally size-fractionated, polydisperse polystyrene microplastic (PS-MP) fractions. P...
Materials today. Bio β 2026 β PMID 42256068
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The Preclinical Effects and Mechanisms of Biofield Therapy on Pancreatic Cancer Cell Growth and Metastasis.
Cell type: HEK293 cells, stem cells, macrophages, keratinocytes, hepatocytes, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, virology
BACKGROUND: The high mortality of pancreatic ductal adenocarcinoma (PDAC) underscores the need for novel treatments. We investigated the potential role of human biofield therapy (BT) in modifying tumorigenic processes in murine and human PDAC cells through a series of inΒ vitro and inΒ vivo studies. METHODS: Cell viability and ultrastructure changes were examined by PrestoBlue assay and Transmission Electron Microscope, respectively. Cell voltage potentials were assessed using DiBAC4 staining. Bot...
Cancer medicine β 2026 β PMID 41968966
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AhR agonist Ficz modulates T follicular helper cells and ameliorates skin fibrosis in a bleomycin-induced SSc mouse model.
Cell type: fibroblasts
Imaging technique: fluorescence microscopy
Disease area: infectious disease, virology, fibrosis, inflammation
OBJECTIVES: T follicular helper (Tfh) cells are involved in the pathogenesis of systemic sclerosis (SSc). Recent investigations have revealed aryl hydrocarbon receptor (AhR) regulated skin fibrosis in SSc. This study aims to explore the effect of AhR agonist Ficz on Tfh in a bleomycin (BLM)-induced SSc mouse model. METHODS: BALB/c mice were randomly assigned to the control group (Ctrl), BLM-induced SSc mouse group (BLM) and the Ficz treatment group (BLM+Ficz). BLM (1βmg/kg/day) and Ficz (1βΒ΅g/mo...
Journal of scleroderma and related disorders β 2026 β PMID 42256627
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Remote stimulation of mitochondria and the antioxidant potential of thymocytes by weak non-ionizing non-thermal broadband pulsed electromagnetic fields in mouse.
Cell type: fibroblasts, macrophages
Imaging technique: fluorescence microscopy
Disease area: cancer, virology, COVID-19
UNLABELLED: Leading medical centers are currently considering pulsed electromagnetic therapy as a promising approach to prolong active life. OBJECTIVE: The aim of this study was to investigate the effects of non-ionizing electromagnetic fields on mitochondrial activity and the state of the lipid peroxidation system, an antioxidant protection system in mice. METHODOLOGY: The studies were carried out on 60 BALB/c mice in accordance with bioethical standards and general ethical principles of animal...
Frontiers in physiology β 2026 β PMID 42078208
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5-methoxyresorcinol mitigates postmenopausal osteoporosis through regulation of the PI3K-AKT-GSK3Ξ² signaling pathway and ROS homeostasis.
Cell type: fibroblasts, macrophages
Imaging technique: fluorescence microscopy
Disease area: cancer, inflammation
OBJECTIVES: Osteoporosis is a systemic skeletal disease characterized by excessive osteoclast-mediated bone resorption. This study aimed to evaluate the therapeutic potential of 5-methoxyresorcinol (MR) in osteoporosis and to clarify its underlying mechanisms. METHODS: Network pharmacology analysis was performed to identify shared targets between MR and osteoporosis. In vitro experiments using bone marrow-derived macrophages were performed to assess the effects of MR on osteoclast differentiatio...
Frontiers in pharmacology β 2026 β PMID 42232183
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Tentonin 3 regulates the proliferation and migration of neural stem cells during embryonic brain development.
Cell type: HEK293 cells, stem cells, neurons, epithelial cells
Imaging technique: confocal microscopy, Z-stack imaging
Disease area: virology
BACKGROUND: Cell fate determination during brain development, encompassing both neural stem cells (NSCs) and immature neurons, is orchestrated by complex mechanical signals. While mechanical sensing during forebrain development is gaining recognition, the underlying regulatory genes remain underexplored. RESULTS: In this study, we present a comprehensive analysis of the expression patterns of the mechanosensitive channel Tentonin 3 (TMEM150C, TTN3) across critical stages of embryonic brain devel...
BMC biology β 2026 β PMID 42010547
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An automated and high-throughput approach for enhanced precision of adenoviral titering.
Cell type: HEK293 cells, A549 lung cancer cells, primary cells, macrophages, neurons
Imaging technique: fluorescence microscopy
Disease area: cancer, infectious disease, virology
Accurate quantification of viral vectors and vaccines is a crucial step required before any downstream use of virus preparations. The conventional immunocytochemistry-based method of adenovirus quantification has been widely used, but there are many areas for improvement toward accuracy and resource consumption savings to reduce viral miscalculation and wastage of vaccination materials. In this work, a one-step approach is implemented for optimized adenoviral quantification that uses a single an...
Molecular therapy. Methods & clinical development β 2025 β PMID 39968184
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HDGF Protects Retinal Pigment Epithelium from Glyoxal-Induced Ferroptosis via SIRT1/PGC-1Ξ±/Nrf2 Pathway.
Cell type: primary cells, neurons, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, inflammation
Age-related macular degeneration (AMD) is driven in part by the accumulation of reactive metabolites like glyoxal (GO), which induces retinal pigment epithelium (RPE) degeneration. Here, we demonstrate that GO triggers ferroptosis in human ARPE-19 cells, as characterized by iron-dependent lipid peroxidation, glutathione depletion, and reactive oxygen species (ROS) accumulation. This ferroptotic cell death is coupled with profound mitochondrial dysfunction, featuring network fragmentation and the...
Antioxidants (Basel, Switzerland) β 2025 β PMID 41462634
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Quantification of timelapse 3D tumor spheroid killing activity of NK cells using a live-cell imaging system.
Cell type: HeLa cells, A549 lung cancer cells, stem cells, iPSCs
Imaging technique: live-cell imaging, time-lapse microscopy, brightfield microscopy
Disease area: cancer, virology
Natural killer (NK) cells are critical components of the immune system, responsible for recognizing and eliminating a wide range of abnormal cells, including those infected by pathogens or transformed into cancerous cells. Their potent cytotoxic functions, encompassing the direct release of cytotoxic granules, antibody-dependent cell cytotoxicity (ADCC), and the expression of apoptosis-inducing ligands, make NK cells a promising therapeutic product in cancer immunotherapy. This study presents a ...
PloS one β 2025 β PMID 41086173
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Oxygen-Releasing Nanodroplets Relieve Intratumoral Hypoxia and Potentiate Photodynamic Therapy in 3D Head and Neck Cancer Spheroids.
Cell type: stem cells
Imaging technique: fluorescence microscopy, time-lapse microscopy
Disease area: cancer, infectious disease, virology
Hypoxia in solid tumors, including head and neck cancer (HNC), contributes to treatment resistance, aggressive tumor phenotypes, and poorer clinical outcomes. Perfluorocarbon nanodroplets have emerged as promising drugs to alleviate tumor hypoxia. These versatile nanocarriers can also encapsulate and deliver various therapeutic agents, offering a multifunctional approach to cancer treatment. However, a detailed characterization of hypoxia alleviation, particularly the duration of hypoxia treatme...
ACS biomaterials science & engineering β 2025 β PMID 40041949
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Cationic Peptoids for Systemic In Vivo Cartilage-Targeting.
Cell type: endothelial cells
Imaging technique: fluorescence microscopy, confocal microscopy
Disease area: cancer, infectious disease, bacterial infection
Although cartilage damage is key to the pathogenesis of many musculoskeletal diseases, including osteoarthritis and rheumatoid arthritis (RA), imaging and drug delivery to cartilage remain a demanding challenge. Cartilage is an avascular tissue with a dense matrix constraining the penetration of imaging and targeting agents. Here, a unique class of cationic peptidomimetics featuring peptoid residue Nlys (N-substituted butyl-amino glycine) is reported for cartilage targeting. Sulfo-Cyanine5 (Cy5)...
Advanced science (Weinheim, Baden-Wurttemberg, Germany) β 2025 β PMID 40886326
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Anti-pyocyanin Antibody Exhibits Cytotoxicity Protective Effects on Macrophages: A Promising Innovative Therapeutic Approach for Pseudomonas aeruginosa Infections.
Cell type: A549 lung cancer cells, HepG2 hepatoma cells, fibroblasts, macrophages, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: cancer, infectious disease, virology, bacterial infection, COVID-19, fibrosis, inflammation
Pseudomonas aeruginosa is considered one of the most threatening pathogens worldwide, due to its high adaptability, which leads to resistance to classical antimicrobials. This fact has driven the development of new therapeutic strategies to reduce multiresistant strains and to minimize infection progression. In this context, the protective effect of a monoclonal antibody (mAb122) specific to pyocyanin (PYO), a key virulence factor of P. aeruginosa, was studied in vitro. Quenching PYO may reduce ...
ACS pharmacology & translational science β 2025 β PMID 41409163
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The de novo DNA methyltransferase 3B is a novel epigenetic regulator of MYC in multiple myeloma, representing a promising therapeutic target to counter relapse.
Cell type: stem cells
Imaging technique: fluorescence microscopy
Disease area: cancer, virology
BACKGROUND: The plasma cell malignancy multiple myeloma (MM) remains incurable due to the inevitable development of drug resistance (DR). Epigenetic modifiers are frequently mutated or deregulated in MM patients, contributing to MM progression and relapse. Overexpression of the de novo DNA methyltransferase 3B (DNMT3B) in MM has been reported, correlating with poor prognosis. However, its exact role in MM cell biology and relapse remains elusive. METHODS: To evaluate the basal expression and pro...
Journal of experimental & clinical cancer research : CR β 2025 β PMID 40241199
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Establishment of chicken muscle and adipogenic cell cultures for cultivated meat production.
Cell type: HeLa cells, primary cells, stem cells, iPSCs, fibroblasts
Imaging technique: fluorescence microscopy
Disease area: virology
INTRODUCTION: Cultured meat seeks to replicate the sensory and nutritional attributes l of conventional meat by developing structured muscle tissue using cell culture. This study focuses on the culture of chicken embryonic and muscle-derived mesenchymal stem cells (MSCs) to derive muscle, and fat, optimizing conditions for differentiation and integration. METHODS: We utilized monolayer and three-dimensional microcarrier-based cultures to produce muscle fibers and adipocytes while maintaining the...
Frontiers in nutrition β 2025 β PMID 41158655
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Facilitating microplastic ingestion in aquatic models: A verified protocol for daphnia magna as a trojan horse vector.
Cell type: cell lines
Imaging technique: fluorescence microscopy
Disease area: general cell biology
Microplastic pollution poses a significant environmental threat due to its persistence, widespread distribution, and inherent toxic potential. Despite the increasing number of publications in this field, a standardized protocol for the laboratory intake of microplastics by Daphnia magna has yet to be established. In this study, we introduce a verified protocol designed to facilitate the ingestion of microplastic particles (MPs) by D. magna, ranging in size from 5-55 Β΅m. This protocol can be furt...
MethodsX β 2024 β PMID 39398536
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A New Methodology for the Quantification of Neutrophil Extracellular Traps in Patient Plasma.
Cell type: cell lines
Imaging technique: confocal microscopy
Disease area: infectious disease, virology, COVID-19
Neutrophil extracellular traps (NETs) are web-like structures made up of decondensed chromatin fibers along with neutrophil granular proteins that are extruded by neutrophils after activation or in response to foreign microorganisms. NETs have been associated with autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis, coronavirus disease 2019 (COVID-19), and others. There are reliable methods available to quantitate NETs from neutrophils, but their...
Bio-protocol β 2023 β PMID 37397793
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