EVOS M7000 in Infectious Disease and Neuroscience Research
Curated literature review of peer-reviewed infectious-disease and neuroscience studies that used the EVOS M7000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
The EVOS M7000 Imaging System supports infection-biology and neurobiology workflows that demand live-cell or endpoint fluorescence and brightfield readouts. The papers below cover viral and bacterial infection models, COVID-19 research, stem-cell-derived neurons, and neurodegeneration assays.
Bioactive factors in endometriosis peritoneal fluid remodel human cardiomyocytes.
Cell type: stem cells, iPSCs, fibroblasts, macrophages, endothelial cells
Imaging technique: fluorescence microscopy
Disease area: infectious disease, COVID-19, cardiovascular disease, inflammation
Endometriosis is associated with increased cardiovascular disease (CVD) risk, yet the cellular basis for this relationship remains unclear. We examined whether peritoneal fluid (PF) from women with endometriosis alters cardiomyocyte behavior in vitro. Human-induced pluripotent stem cell-derived cardiomyocytes were exposed for 48 h to standard or hypertrophic media supplemented with peritoneal fluid from endometriosis or control patients. Beating frequency was measured using calcium transient ima...
American journal of physiology. Heart and circulatory physiology — 2026 — PMID 42172437
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Addition of lactic acid bacteria to diluted ram semen as vehicle for vaginal inoculation: interaction with seminal microbiota, sperm quality and antibacterial in vitro effect against Mycoplasma agalactiae.
Cell type: cell lines
Imaging technique: fluorescence microscopy
Disease area: infectious disease, bacterial infection
BACKGROUND: The use of lactic acid bacteria (LAB) as a probiotic in seminal doses of small ruminants has been suggested due to its antibacterial effects against pathogens such as Mycoplasma agalactiae (Ma) and positive effects on ovine vaginal health. The present in vitro study evaluates the antibacterial effect against Ma of a wild LAB strain from caprine prepuce and a commercial human probiotic (L3) composed by Lactobacillus spp., and evaluates their impact on sperm quality, DNA fragmentation,...
BMC veterinary research — 2026 — PMID 42087183
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Authentic Phosphorylation of α-Synuclein at Ser129 Reveals Functional Differences Not Captured by the S129D Phosphomimetic.
Cell type: SH-SY5Y neuroblastoma cells, neurons
Imaging technique: fluorescence microscopy
Disease area: Alzheimer's disease, Parkinson's disease, bacterial infection
Phosphorylation at serine 129 (pS129) is a dominant post-translational modification of α-synuclein (αSyn) and a widely used pathological marker in Parkinson's disease, yet its mechanistic consequences remain debated across physiological and pathological contexts. Most studies rely on phosphomimetic substitutions such as S129D, which approximate net charge but do not reproduce the steric, geometric, or hydrogen-bonding properties of authentic phosphorylation. Here, we establish a robust bacterial...
ACS chemical biology — 2026 — PMID 42024867
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Fabrication of High-Density Multimodal Neural Probes Based on Heterogeneously Integrated CMOS.
Cell type: neurons
Imaging technique: fluorescence microscopy
Disease area: Alzheimer's disease, inflammation
Neural interfaces that simultaneously capture electrical activity and cell-type-specific dynamics across molecularly defined populations are essential for understanding brain function. Monitoring these neural activities could help understand the complex mechanisms and disorders of the brain. While micro-electro-mechanical systems (MEMS) based neural probes provide multi-sensor integration, their lack of on-chip signal processing limits the channel density. On the other hand, complementary metal-...
Advanced science (Weinheim, Baden-Wurttemberg, Germany) — 2026 — PMID 41874523
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Peering Inside the Black Box: Explainable AI to Interpret Advanced Computer Vision Fungal Pathogen Prediction.
Cell type: cell lines
Imaging technique: time-lapse microscopy, brightfield microscopy
Disease area: infectious disease, bacterial infection, COVID-19
Antimicrobial resistance is a growing concern, with pathogenic fungi making a substantial contribution to untreatable life-threatening infections across the globe. Artificial intelligence is increasingly used in microbiology and antimicrobial resistance research, with promise to improve clinical diagnostics and infectious disease treatments. However, how AI models make predictions remains largely unknown, which is a major hurdle for human trust and regulatory approval. We train vision transforme...
Scientific reports — 2026 — PMID 42000833
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Universal conditional knockout approach to multiple species, from fish to human induced pluripotent stem cells.
Cell type: stem cells, iPSCs, neurons
Imaging technique: fluorescence microscopy, confocal microscopy
Disease area: infectious disease, virology
Investigating essential gene function in vertebrate development and disease is challenging due to associated lethality, necessitating precise conditional inactivation. While existing conditional knockout methods can be inefficient and demanding, especially in models like zebrafish and human induced pluripotent stem cells (iPSCs), a widely applicable system has remained elusive. Here, we demonstrate the expanded utility and versatility of a Short Conditional intrON (SCON) knockout cassette, now v...
Nucleic acids research — 2026 — PMID 42132111
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miR-181a post-transcriptionally targets GRK2 to limit maladaptive signaling in cardiomyocytes.
Cell type: fibroblasts, endothelial cells
Imaging technique: fluorescence microscopy
Disease area: virology, cardiovascular disease, fibrosis, inflammation
BACKGROUND: Pathological upregulation of G protein-coupled receptor kinase 2 (GRK2) is a hallmark of heart failure and contributes to maladaptive signaling, hypertrophic remodeling, and cardiomyocyte death. MicroRNAs (miRNAs) are key post-transcriptional regulators of cardiac stress responses, however whether GRK2 is subject to miRNA targeting has not yet been fully established. METHODS: miRNA microarray profiling was performed on mouse hearts two weeks after myocardial infarction. Bioinformatic...
Frontiers in cardiovascular medicine — 2026 — PMID 42099781
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Fentanyl blockade of K+ channels contributes to wooden chest syndrome.
Cell type: HEK293 cells, neurons
Imaging technique: confocal microscopy
Disease area: virology
Fentanyl is widely used perioperatively and illicitly as a drug of abuse. As a potent μ-opioid receptor agonist, fentanyl canonically inhibits excitability through Gαi/o intracellular signalling pathways resulting in analgesia and respiratory depression. However, fentanyl also paradoxically activates respiratory muscles causing a potentially lethal effect termed wooden chest syndrome. Here we show that fentanyl, but not morphine, causes a persistent tonic component of diaphragmatic muscle activi...
The Journal of physiology — 2026 — PMID 41110851
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Photochemical nanomotors reverse anxiety- and depressive-related behaviors in rodents via spatiotemporal polarity dynamics tuning.
Cell type: iPSCs, neurons
Imaging technique: time-lapse microscopy, confocal microscopy
Disease area: infectious disease, virology, inflammation, drug screening
Anxiety and mood disorders, notably major depressive disorder characterized by suppressed neuronal excitability, involve disruption of endogenous molecular polarity homeostasis. Conventional monoamine antidepressants, constrained by delayed therapeutic onset and high risk of drug resistance, neglect and fail to address this pathological feature. Here, we report IC@His-ICG, a near-infrared-responsive photochemical nanomotor that enables polarity switching. Leveraging photochemically driven polari...
Nature communications — 2026 — PMID 41748578
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Retinoic acid inhibits the infection of porcine reproductive and respiratory syndrome virus.
Cell type: HEK293 cells, stem cells, macrophages, epithelial cells
Imaging technique: fluorescence microscopy
Disease area: infectious disease, virology, COVID-19, inflammation
INTRODUCTION: Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen threatening the global swine industry, with existing vaccines failing to provide broad protection due to the high viral genetic variability and immune evasion capacity. Retinoic acid (RA), a vitamin A metabolite known for immunomodulatory functions, has been reported to enhance antiviral innate immunity and suppress excessive inflammation. However, whether RA affects PRRSV infection remains unclear. MET...
Frontiers in veterinary science — 2026 — PMID 41953114
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Micropatterned DNA Hydrogels for Spatiotemporal Programming of Chemical Reaction Networks.
Cell type: stem cells, iPSCs
Imaging technique: fluorescence microscopy, time-lapse microscopy, confocal microscopy, Z-stack imaging
Disease area: bacterial infection
Cells in living systems communicate by sending and receiving signal molecules to coordinate their behavior. To achieve long-distance and noise-resistant communication, cells pattern themselves into spatially organized structures. Inspired by this strategy, systems biology and materials science have aimed to construct artificial communication systems whose dynamics can be controlled by their spatial arrangement. However, experimental understanding of how spatial arrangement influences communicati...
ACS nano — 2026 — PMID 41830802
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Single-cell transcriptomic analyses reveal angiogenic vascular responses to chronic cerebral hypoperfusion.
Cell type: stem cells, fibroblasts, macrophages, neurons, endothelial cells, epithelial cells
Imaging technique: confocal microscopy
Disease area: Alzheimer's disease, diabetes, inflammation
Elucidating the mechanisms underlying vascular injury and repair may guide development of therapies against vascular cognitive impairment and dementia (VD). We employed single-cell RNA sequencing to map cell populations and explore vascular responses in mouse brains collected 42 days after asymmetric common carotid artery stenosis (ACAS)-induced chronic cerebral hypoperfusion. A unique tip cell type was identified among endothelial cell (EC) clusters and validated by immunostaining. Gene Ontolog...
iScience — 2026 — PMID 41907427
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Structural insights into functional regulation of the human CPEB3 prion by an amyloid-forming segment.
Cell type: HeLa cells, HEK293 cells, neurons
Imaging technique: confocal microscopy, Z-stack imaging
Disease area: Alzheimer's disease, virology, bacterial infection
The cytoplasmic polyadenylation-element-binding-protein-3 (CPEB3) is a functional prion thought to modulate protein synthesis and enable consolidation of long-term memory in neurons. We report a cryoelectron microscopy (cryo-EM) structure of amyloid fibrils grown in vitro from the first prion-like domain of human CPEB3 (hCPEB3), revealing their ordered 49-residue core, spanning L103 to F151. CPEB3 lacking that segment coalesces into abnormal puncta in cells compared to wild-type CPEB3, localizes...
Structure (London, England : 1993) — 2025 — PMID 40480223
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Corneal Endothelium Regeneration with Decellularized Porcine Corneal Extracellular Matrix Scaffolds.
Cell type: stem cells, endothelial cells
Imaging technique: fluorescence microscopy
Disease area: virology
BACKGROUND: To evaluate the structural, biochemical, and functional performance of decellularized porcine corneal extracellular matrix (dECM) scaffolds for engineering human corneal endothelium. METHODS: Porcine corneas were decellularized using either 0.3% sodium dodecyl sulfate (SDS) or 1.5 M sodium chloride (NaCl), followed by enzymatic nucleic acid digestion. Histological and biochemical analyses were performed to assess decellularization efficiency and extracellular matrix preservation. Hum...
Tissue engineering and regenerative medicine — 2025 — PMID 40536661
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Novel reporter constructs to accelerate antiviral and therapeutic discovery for Enterovirus-A71.
Cell type: HEK293 cells, stem cells, iPSCs, epithelial cells
Imaging technique: time-lapse microscopy, confocal microscopy, Z-stack imaging
Disease area: infectious disease, virology, bacterial infection
Enterovirus A71 (EV-A71) is an important human pathogen and 'prototype pathogen' for studies of other Enteroviruses of pandemic potential. Understanding the biology of EV-A71 would inform generalizable strategies for antiviral drug, vaccine, and monoclonal antibody development. Such studies are accelerated by robust reagents to evaluate efficacy. Here, we describe and evaluate a suite of synthetic reporter constructs to accelerate EV-A71 research and therapeutic discovery. These constructs inclu...
Antiviral research — 2025 — PMID 39900143
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