EVOS M5000 in Cell Biology & Signalling: Independent Studies — Wave 3 Part 1

Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.

EVOS M5000IndependentWave 3

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Mesenchymal Stem Cells As Guideposts for Nanoparticle-Mediated Targeted Drug Delivery in Ovarian Cancer.

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Nanocarriers have been extensively utilized for the systemic targeting of various solid tumors and their metastases. However, current drug delivery systems, in general, suffer from a lack of selectivity for tumor cells. Here, we develop a novel two-step targeting strategy that relies on the selective accumulation of targetable synthetic receptors (i.e., azide moieties) in tumor

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Combining Bioinformatics and Experiments to Identify

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Aging of functional ovaries occurs many years before aging of other organs in the female body. In recent years, a greater number of women continue to postpone their pregnancies to later stages in their lives, raising concerns of the effect of ovarian aging. Mitochondria play an important role in the connection between the aging granulosa cells and oocytes. However, the underlyi

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Inverse regulation of claudin-2 and -7 expression by p53 and hepatocyte nuclear factor 4α in colonic MCE301 cells.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Colonic epithelial cells move up along the crypt villus axis and are differentiated into absorptive or secretory cells. Claudin-7 (CLDN7), a tight junctional protein, is mainly located at the surface of crypt, whereas CLDN2 is located at the bottom. However, the expression mechanism and function of these CLDNs are not fully understood. The expression levels of CLDN2 and CLDN7 w

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Combined ASBT Inhibitor and FGF15 Treatment Improves Therapeutic Efficacy in Experimental Nonalcoholic Steatohepatitis.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Pharmacologic agents targeting bile acid signaling show promise for treating nonalcoholic steatohepatitis (NASH). However, clinical findings suggest that new treatment strategies with enhanced therapeutic efficacy and minimized undesired effects are needed. This preclinical study investigates whether combining an apical sodium-bile acid transporter (ASBT) inhibitor GSK233072 (G

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Effect of F16-Betulin Conjugate on Mitochondrial Membranes and Its Role in Cell Death Initiation.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

This work demonstrates the effects of a newly synthesized conjugate of the plant triterpenoid betulin and the penetrating cation F16 used for mitochondrial targeting. The resulting F16-betulin conjugate revealed a mitochondria-targeted effect, decreasing the mitochondrial potential and inducing superoxide overproduction in rat thymocytes

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Caveolin-1 mediates soft scaffold-enhanced adipogenesis of human mesenchymal stem cells.

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Human bone marrow-derived mesenchymal stem cells (hBMSCs) can differentiate into adipocytes upon stimulation and are considered an appropriate cell source for adipose tissue engineering. In addition to biochemical cues, the stiffness of a substrate that cells attach to has also been shown to affect hBMSC differentiation potential. Of note, most current studies are conducted on

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Cytotoxic and apoptotic data of BPA and BPA alternatives TMBPF, BPAF, and BPS in female adult rat and human stem cells.

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Here, we used female adult rat adipose-derived stem cells (rASCs) and human adipose-derived stem cells (hASCs) to compare the toxicities and potencies of several widespread environmental toxins that may be endocrine-disrupting chemicals, including bisphenol A (BPA), and the newer BPA alternatives bisphenol S (BPS), bisphenol AF (BPAF), and tetramethyl bisphenol F (TMBPF). Adult

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R-Ras subfamily proteins elicit distinct physiologic effects and phosphoproteome alterations in neurofibromin-null MPNST cells.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Loss of the Ras GTPase-activating protein neurofibromin promotes nervous system tumor pathogenesis in patients with neurofibromatosis type 1 (NF1). Neurofibromin loss potentially hyperactivates classic Ras (H-Ras, N-Ras, K-Ras), M-Ras, and R-Ras (R-Ras, R-Ras2/TC21) subfamily proteins. We have shown that classic Ras proteins promote proliferation and survival, but not migration

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Threshold of heteroplasmic truncating MT-ATP6 mutation in reprogramming, Notch hyperactivation and motor neuron metabolism.

Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Mutations in mitochondrial DNA encoded subunit of ATP synthase, MT-ATP6, are frequent causes of neurological mitochondrial diseases with a range of phenotypes from Leigh syndrome and NARP to ataxias and neuropathies. Here we investigated the functional consequences of an unusual heteroplasmic truncating mutation m.9154C>T in MT-ATP6, which caused peripheral neuropathy, ataxia a

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Characterization of primary models of human trophoblast.

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Two recently developed models, trophoblast organoids and trophoblast stem cells (TSCs), are useful tools to further the understanding of human placental development. Both differentiate from villous cytotrophoblast (VCT) to either extravillous trophoblast (EVT) or syncytiotrophoblast (SCT). Here, we compare the transcriptomes and miRNA profiles of these models to identify which

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1-Deoxysphingolipids, Early Predictors of Type 2 Diabetes, Compromise the Functionality of Skeletal Myoblasts.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Metabolic dysfunction, dysregulated differentiation, and atrophy of skeletal muscle occur as part of a cluster of abnormalities associated with the development of Type 2 diabetes mellitus (T2DM). Recent interest has turned to the attention of the role of 1-deoxysphingolipids (1-DSL), atypical class of sphingolipids which are found significantly elevated in patients diagnosed wi

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Fucoidan Isolated from

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Recent studies have revealed that marine brown seaweeds contain numerous bioactive compounds which exhibit various bioactivities. The present study investigated the effect of low molecular weight fucoidan (SCF) isolated from

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Improving the Safety of Mesenchymal Stem Cell-Based

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Human mesenchymal stem cells (MSCs) are multipotent stem cells that have been intensively studied as therapeutic tools for a variety of disorders. To enhance the efficacy of MSCs, therapeutic genes are introduced using retroviral and lentiviral vectors. However, serious adverse events (SAEs) such as tumorigenesis can be induced by insertional mutagenesis. We generated lentivira

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Phytoglobin Expression Alters the Na

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Soybean (

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Chemical editing of proteoglycan architecture.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Proteoglycans are heterogeneous macromolecular glycoconjugates that orchestrate many important cellular processes. While much attention has focused on the poly-sulfated glycosaminoglycan chains that decorate proteoglycans, other important elements of their architecture, such as core proteins and membrane localization, have garnered less emphasis. Hence, comprehensive structure-

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Optogenetic control of NOTCH1 signaling.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

The Notch signaling pathway is a crucial regulator of cell differentiation as well as tissue organization, whose deregulation is linked to the pathogenesis of different diseases. NOTCH1 plays a key role in breast cancer progression by increasing proliferation, maintenance of cancer stem cells, and impairment of cell death. NOTCH1 is a mechanosensitive receptor, where mechanical

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Comparative Efficiency for in vitro Transfection of Goat Undifferentiated Spermatogonia Using Lipofectamine Reagents and Electroporation.

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Spermatogonial stem cells (SSC), also referred to as undifferentiated spermatogonia, are the germline stem cells responsible for continuous spermatogenesis throughout a male's life. They are, therefore, an ideal target for gene editing. Previously, SSC from animal testis have been isolated and transplanted to homologous recipients resulting in the successful reestablishment of

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Testing 3D printed biological platform for advancing simulated microgravity and space mechanobiology research.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

The advancement of microgravity simulators is helping many researchers better understanding the impact of the mechanically unloaded space environment on cellular function and disfunction. However, performing microgravity experiments on Earth, using simulators such as the Random Positioning Machine, introduces some unique practical challenges, including air bubble formation and

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<i>Fusobacterium necrophorum</i> Promotes Apoptosis and Inflammatory Cytokine Production Through the Activation of NF-κB and Death Receptor Signaling Pathways.

Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

<i>Fusobacterium necrophorum</i> can cause liver abscess, foot rot in ruminants, and Lemire syndrome in humans, Also, its virulence factors can induce the apoptosis of macrophages and neutrophils. However, the detailed mechanism has not been fully clarified. This study investigated the mechanisms of apoptosis and inflammatory factor production in <i>F. necrophorum</i>-induced n

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An integrated atlas of human placental development delineates essential regulators of trophoblast stem cells.

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

The trophoblast lineage safeguards fetal development by mediating embryo implantation, immune tolerance, nutritional supply and gas exchange. Human trophoblast stem cells (hTSCs) provide a platform to study lineage specification of placental tissues; however, the regulatory network controlling self-renewal remains elusive. Here, we present a single-cell atlas of human trophobla

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Comet assay for quantification of the increased DNA damage burden in primary human chondrocytes with aging and osteoarthritis.

Cell type: Chondrocyte | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

It is known that chondrocytes from joints with osteoarthritis (OA) exhibit high levels of DNA damage, but the degree to which chondrocytes accumulate DNA damage during "normal aging" has not been established. The goal of this study was to quantify the DNA damage present in chondrocytes obtained from cadaveric donors of a wide age range, and to compare the extent of this damage

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Mesenchymal stem cell spheroids alleviate neuropathic pain by modulating chronic inflammatory response genes.

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Chronic neuropathic pain is caused by dysfunction of the peripheral nerves associated with the somatosensory system. Mesenchymal stem cells (MSCs) have attracted attention as promising cell therapeutics for chronic pain; however, their clinical application has been hampered by the poor

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MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Pulmonary fibrosis is characterized by the destruction of alveolar architecture and the irreversible scarring of lung parenchyma, with few therapeutic options and effective therapeutic drugs. Here, we demonstrate the anti-pulmonary fibrosis of 3-(4-methoxyphenyl)-4-oxo-4

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Label-Free Microfluidic Impedance Cytometry for Acrosome Integrity Assessment of Boar Spermatozoa.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Microfluidics and lab-on-chip technologies have been used in a wide range of biomedical applications. They are known as versatile, rapid, and low-cost alternatives for expensive equipment and time-intensive processing. The veterinary industry and human fertility clinics could greatly benefit from label-free and standardized methods for semen analysis. We developed a tool to det

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Diesel exhaust particles induce human umbilical vein endothelial cells apoptosis by accumulation of autophagosomes and caspase-8 activation.

Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Diesel exhaust particles (DEP) are risk factors for endothelial cells (ECs) dysfunction. However, the mechanism by which DEP induce ECs apoptosis remains unclear. Here, we investigated how DEP induce death of human umbilical vein ECs (HUVECs), with a focus on the autophagy-mediated apoptotic pathway. DEP induced dose-dependent HUVECs death and exposure to the IC

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BK

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Duchenne muscular dystrophy (DMD) is a progressive hereditary disease caused by the absence of the dystrophin protein. This is secondarily accompanied by a dysregulation of ion homeostasis, in which mitochondria play an important role. In the present work, we show that mitochondrial dysfunction in the skeletal muscles of dystrophin-deficient

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LPAR1 and aberrantly expressed LPAR3 differentially promote the migration and proliferation of malignant peripheral nerve sheath tumor cells.

Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Schwann cell-derived neoplasms known as malignant peripheral nerve sheath tumors (MPNSTs) are the most common malignancy and the leading cause of death in individuals with neurofibromatosis Type 1. Using genome-scale shRNA screens, we have previously found evidence suggesting that lysophosphatidic acid receptors (LPARs) are essential for MPNST proliferation and/or survival. Her

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Protective effects of Gypenoside XVII against cerebral ischemia/reperfusion injury via SIRT1-FOXO3A- and Hif1a-BNIP3-mediated mitochondrial autophagy.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Mitochondrial autophagy maintains mitochondrial function and cellular homeostasis and plays a critical role in the pathological process of cerebral ischemia/reperfusion injury (CIRI). Whether Gypenoside XVII (GP17) has regulatory effects on mitochondrial autophagy against CIRI remains unclear. The purpose of this study was to investigate the pharmacodynamic effects and mechanis

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DNA damage-induced cellular senescence is regulated by 53BP1 accumulation in the nuclear foci and phase separation.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Cellular senescence is linked to a wide range of age-related diseases and can be triggered by a variety of stresses, including DNA damage. A variety of genotoxic stressors, such as anti-cancer drugs, cause DNA double-strand breaks (DSBs), which trigger the accumulation of the tumour suppressor protein p53 in the nucleus. Cellular stresses stabilize and activate the p53 signalli

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Nanotopography reveals metabolites that maintain the immunomodulatory phenotype of mesenchymal stromal cells.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Mesenchymal stromal cells (MSCs) are multipotent progenitor cells that are of considerable clinical potential in transplantation and anti-inflammatory therapies due to their capacity for tissue repair and immunomodulation. However, MSCs rapidly differentiate once in culture, making their large-scale expansion for use in immunomodulatory therapies challenging. Although the diffe

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Ca

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Ca

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<i>Olea europaea</i> Leaf Phenolics Oleuropein, Hydroxytyrosol, Tyrosol, and Rutin Induce Apoptosis and Additionally Affect Temozolomide against Glioblastoma: In Particular, Oleuropein Inhibits Spheroid Growth by Attenuating Stem-like Cell Phenotype.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

The effects of

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PAI-1 mediates TGF-β1-induced myofibroblast activation in tenocytes via mTOR signaling.

Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Transforming growth factor-beta (TGF-β1) induces plasminogen activator inhibitor 1 (PAI-1) to effect fibrotic pathologies in several organs including tendon. Recent data implicated PAI-1 with inhibition of phosphatase and tensin homolog (PTEN) suggesting that PAI-1-induced adhesions involves phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin (mTOR) signali

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Establishment of gastrointestinal assembloids to study the interplay between epithelial crypts and their mesenchymal niche.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

The cellular organization of gastrointestinal crypts is orchestrated by different cells of the stromal niche but available in vitro models fail to fully recapitulate the interplay between epithelium and stroma. Here, we establish a colon assembloid system comprising the epithelium and diverse stromal cell subtypes. These assembloids recapitulate the development of mature crypts

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Differentiation of stem cell-derived pancreatic progenitors into insulin-secreting islet clusters in a multiwell-based static 3D culture system.

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Orbital shaker-based suspension culture systems have been in widespread use for differentiating human pluripotent stem cell (hPSC)-derived pancreatic progenitors toward islet-like clusters during endocrine induction stages. However, reproducibility between experiments is hampered by variable degrees of cell loss in shaking cultures, which contributes to variable differentiation

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NLRX1 functions as a tumor suppressor in Pan02 pancreatic cancer cells.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Pancreatic cancer is a deadly malignancy with limited treatment options. NLRX1 is a unique, understudied member of the Nod-like Receptor (NLR) family of pattern recognition receptors that regulates a variety of biological processes that are highly relevant to pancreatic cancer. The role of NLRX1 in cancer remains highly enigmatic, with some studies defining its roles as a tumor

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Glycine-β-Muricholic Acid Improves Liver Fibrosis and Gut Barrier Function by Reducing Bile Acid Pool Size and Hydrophobicity in Male

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

<i>Cyp2c70</i> knockout mice lack the enzyme that produces muricholic acids and show a "human-like" hydrophobic bile acid pool-induced hepatobiliary injury. In this study, we investigated the potential anti-cholestasis effect of glycine-conjugated β muricholic acid (G-β-MCA) in male <i>Cyp2c70</i> KO mice based on its hydrophilic physiochemical property and signaling property a

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Clodronate Liposome-Mediated Phagocytic Hemocyte Depletion Affects the Regeneration of the Cephalic Tentacle of the Invasive Snail,

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

After amputation, granular hemocytes infiltrate the blastema of regenerating cephalic tentacles of the freshwater snail

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Rapid Characterization of the Functional and Pharmacological Consequences of Cantú Syndrome K

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Gain-of-function of K

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Human Hepatocellular response in Cholestatic Liver Diseases.

Cell type: Hepatocyte | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC), the most common types of cholestatic liver disease (CLD), result in enterohepatic obstruction, bile acid accumulation, and hepatotoxicity. The mechanisms by which hepatocytes respond to and cope with CLD remain largely unexplored. This study includes the characterization of hepatocytes isolated from exp

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Investigation of Cobalt(III) Cage Complexes as Inhibitors of the Mitochondrial Calcium Uniporter.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

The mitochondrial calcium uniporter (MCU) mediates uptake of calcium ions (Ca

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Combined Treatment of

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Mori Folium (

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Evaluation of polyetheretherketone composites modified by calcium silicate and carbon nanotubes for bone regeneration: mechanical properties, biomineralization and induction of osteoblasts.

Cell type: Osteoblast | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Desired orthopedic implant materials must have a good biological activity and possess appropriate mechanical property that correspond to those of human bone. Although polyetheretherketone (PEEK) has displayed a promising application prospect in musculoskeletal and dentistry reconstruction thanks to its non-biodegradability and good biocompatibility in the body, the poor osseoin

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Investigating proliferation and differentiation capacities of Hanwoo steer myosatellite cells at different passages for developing cell-cultured meat.

Cell type: Cell culture models | Technique: Fluorescence imaging, live-cell | Disease/area: Cell Biology & Signalling

The aim of study was to investigate proliferation and differentiation capacities of Hanwoo myosatellite cells for the development of Hanwoo cell cultures. From P1 to P19, the number of live cells decreased and the cell size increased. It was confirmed that the PAX7 mRNA was higher in P3 than P6 and P9 (p < 0.05). The maximum differentiation score was measured from P1 to P12. Th

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Xuebijing Injection Attenuates Heat Stroke-Induced Brain Injury through Oxidative Stress Blockage and Parthanatos Modulation via PARP-1/AIF Signaling.

Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Heat stroke (HS) is a potentially fatal acute condition caused by an interplay of complex events including inflammation, endothelial injury, and coagulation abnormalities that make its pharmacological treatment a challenging problem. The traditional Chinese medicine Xuebijing injection (XBJ) has been shown to reduce inflammatory responses and prevent organ injuries in HS-induce

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Investigating the cell membrane localization of PADI4 in breast cancer cells and inhibition of anti-PADI4 monoclonal antibody.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Peptidyl arginine deiminase 4 (PADI4) is a post-translational modification enzymecan that converts arginine in protein into citrulline in the presence of calcium ions, which is called citrullination. PADI4 has been reported to be expressed in the cytoplasm and nucleus in a variety of malignant tumors. Based on the GeneCards database and our previous research, it is speculated t

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Senolytic Treatment Improve Small Intestine Regeneration in Aging.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Aging induces a series of alterations, specifically a decline in the stature and number of villi and crypts in the small intestine, thus compromising the absorbent capability of the villi. This investigation employed a senolytic combination of dasatinib and quercetin (D+Q) to examine its impact on the intestinal tract of elderly mice. Our findings demonstrate that D+Q treatment

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Spatial Transcriptomics Suggests That Alterations Occur in the Preneoplastic Breast Microenvironment of BRCA1/2 Mutation Carriers.

Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Breast cancer is the most common cancer in females, affecting one in every eight women and accounting for the majority of cancer-related deaths in women worldwide. Germline mutations in the BRCA1 and BRCA2 genes are significant risk factors for specific subtypes of breast cancer. BRCA1 mutations are associated with basal-like breast cancers, whereas BRCA2 mutations are associat

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Effect of Fusidic Acid and Some Nitrogen-Containing Derivatives on Liposomal and Mitochondrial Membranes.

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

The paper assesses the membranotropic action of the natural antibiotic fusidic acid (FA) and its derivatives. It was found that a FA analogue with ethylenediamine moiety (derivative 2), in contrast to native FA and 3,11-dioxime analogue (derivative 1), is able to increase the mobility of the lipid bilayer in the zone of lipid headgroups, as well as to induce permeabilization of

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Effect of

Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Palm oil (PO) is currently the most widely used fat source for food production, but insect fat from

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Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modeling.

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

The capability to generate induced pluripotent stem cell (iPSC) lines, in tandem with CRISPR-Cas9 DNA editing, offers great promise to understand the underlying genetic mechanisms of human disease. The low efficiency of available methods for homogeneous expansion of singularized CRISPR-transfected iPSCs necessitates the coculture of transfected cells in mixed populations and/or

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Oncogenic Cells of Renal Embryonic Lineage Sensitive to the Small-Molecule Inhibitor QC6352 Display Depletion of KDM4 Levels and Disruption of Ribosome Biogenesis.

Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

The histone lysine demethylases KDM4A-C are involved in physiologic processes including stem cell identity and self-renewal during development, DNA damage repair, and cell-cycle progression. KDM4A-C are overexpressed and associated with malignant cell behavior in multiple human cancers and are therefore potential therapeutic targets. Given the role of KDM4A-C in development and

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Lycopene Maintains Mitochondrial Homeostasis to Counteract the Enterotoxicity of Deoxynivalenol.

Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

The intestinal tract is a target organ for Deoxynivalenol (DON) absorption and toxicity. Mitochondrial homeostasis imbalance is the gut toxicity mechanism of DON. Lycopene (LYC) has intestinal protective effects and can maintain mitochondrial homeostasis in response to various danger signals. The purpose of this study was to explore the protective effect of LYC on DON-induced I

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Repli-seq Sample Preparation using Cell Sorting with Cell-Permeant Dyes.

Cell type: Cell culture models | Technique: Fluorescence imaging, live-cell | Disease/area: Cell Biology & Signalling

Replication timing is significantly correlated with gene expression and chromatin organization, changes dynamically during cell differentiation, and is altered in diseased states. Genome-wide analysis of replication timing is performed in actively replicating cells by Repli-seq. Current methods for Repli-seq require cells to be fixed in large numbers. This is a barrier for samp

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Regulation of myogenesis and adipogenesis by the electromagnetic perceptive gene.

Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling

Obesity has been increasing in many regions of the world, including Europe, USA, and Korea. To manage obesity, we should consider it as a disease and apply therapeutic methods for its treatment. Molecular and therapeutic approaches for obesity management involve regulating biomolecules such as DNA, RNA, and protein in adipose-derived stem cells to prevent to be fat cells. Multi

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