EVOS M5000 in Cell Biology Research — Wave 3 Part 2
Independent papers using the EVOS M5000 Imaging System for cell lines, signalling, organelle imaging and general cell biology. Each entry was discovered independently of Thermo Fisher's curated EVOS M5000 citations and verified to mention the EVOS M5000 Imaging System as a microscope in the full text. Paper cards link to Google Scholar, PubMed, PMC and DOI when available.
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Proximity-dependent biotinylation reveals an interaction between ubiquitin-specific peptidase 46 and centrosome-related proteins.
Cell type: HEK293, cell line | Technique: EdU proliferation | Disease/area: general | FEBS open bio (2025)
Protein ubiquitination extensively modulates protein functions and controls various biological processes, such as protein degradation, signal transduction, transcription, and DNA repair. Ubiquitination is a reversible post-translational modification, and deubi
Google Scholar | PubMed | PMC | DOI
A TLR7 Agonist Conjugated to a Nanofibrous Peptide Hydrogel as a Potent Vaccine Adjuvant.
Cell type: HEK293 | Technique: general | Disease/area: general | Advanced healthcare materials (2025)
Toll-like receptors (TLRs) recognize pathogen- and damage-associated molecular patterns and, in turn, trigger the release of cytokines and other immunostimulatory molecules. As a result, TLR agonists are increasingly being investigated as vaccine adjuvants. Ma
Google Scholar | PubMed | PMC | DOI
Antioxidant and Laxative Effects of Methanol Extracts of Green Pine Cones (
Cell type: general | Technique: general | Disease/area: general | Antioxidants (Basel, Switzerland) (2024)
Oxidative stress is the key cause of the etiopathogenesis of several diseases associated with constipation. This study examined whether the green pine cone can improve the symptoms of constipation based on the antioxidant activities. The changes in the key par
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iPSC-derived human sensory neurons reveal a subset of TRPV1 antagonists as anti-pruritic compounds.
Cell type: iPSC, neuron | Technique: general | Disease/area: general | Scientific reports (2024)
Signaling interplay between the histamine 1 receptor (H1R) and transient receptor potential cation channel subfamily V member 1 (TRPV1) in mediating histaminergic itch has been well-established in mammalian models, but whether this is conserved in humans remai
Google Scholar | PubMed | PMC | DOI
Transcriptomic and lipidomic profiling reveals distinct bioactive lipid signatures in slow and fast muscles and highlights the role of resolvin-D2 in fiber type determination during myogenesis.
Cell type: general | Technique: general | Disease/area: general | FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2024)
Skeletal muscles are predominantly composed of long, multinucleated muscle fibers, classified according to their metabolic and contractile phenotype. The determination of fiber types is influenced by various factors (e.g., innervation, hormones, physical deman
Google Scholar | PubMed | PMC | DOI
EV-Elute: A universal platform for the enrichment of functional surface marker-defined extracellular vesicle subpopulations.
Cell type: endothelial | Technique: general | Disease/area: general | Journal of extracellular vesicles (2024)
Intercellular communication via extracellular vesicles (EVs) has been identified as a vital component of a steadily expanding number of physiological and pathological processes. To accommodate these roles, EVs have highly heterogeneous molecular compositions.
Google Scholar | PubMed | PMC | DOI
Design principles to tailor Hsp104 therapeutics.
Cell type: HEK293 | Technique: EdU proliferation | Disease/area: general | Cell reports (2024)
The hexameric AAA+ disaggregase, Hsp104, collaborates with Hsp70 and Hsp40 via its autoregulatory middle domain (MD) to solubilize aggregated proteins. However, how ATP- or ADP-specific MD configurations regulate Hsp104 hexamers remains poorly understood. Here
Google Scholar | PubMed | PMC | DOI
Impact of Drying on Phytonutritional Compounds, In Vitro Antioxidant Activity and Cytotoxicity of Spiny Saltbush (
Cell type: general | Technique: general | Disease/area: general | Antioxidants (Basel, Switzerland) (2024)
The Spiny saltbush (
Google Scholar | PubMed | PMC | DOI
Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from
Cell type: general | Technique: general | Disease/area: general | Antioxidants (Basel, Switzerland) (2024)
The present study investigated the photoprotective effect of the ultrasonic-assisted ethanol extract (USHE) from
Google Scholar | PubMed | PMC | DOI
High-throughput expansion microscopy enables scalable super-resolution imaging.
Cell type: cardiomyocyte | Technique: confocal | Disease/area: general | eLife (2024)
Expansion microscopy (ExM) enables nanoscale imaging using a standard confocal microscope through the physical, isotropic expansion of fixed immunolabeled specimens. ExM is widely employed to image proteins, nucleic acids, and lipid membranes in single cells;
Google Scholar | PubMed | PMC | DOI
Correlative light and soft X-ray tomography of in situ mesoscale heterochromatin structure in intact cells.
Cell type: T cell, cell line, fibroblast | Technique: general | Disease/area: general | Scientific reports (2024)
Heterochromatin organization is critical to many genome-related programs including transcriptional silencing and DNA repair. While super-resolution imaging, electron microscopy, and multiomics methods have provided indirect insights into the heterochromatin or
Google Scholar | PubMed | PMC | DOI
Comparative Analysis of Commercial and Home-Made Media on RSPO1/S6R Axis in Organoids with Different Wnt Backgrounds: A Methodological Guide for the Selection of Intestinal Patient-Derived Organoids Culture Media.
Cell type: general | Technique: general | Disease/area: general | International journal of molecular sciences (2024)
WNT3A is an intestinal ligand triggering the Wnt/β-catenin (Wnt) pathway, which can be enhanced by R-spondin 1 (RSPO1) through the RSPO1-LGR axis or antagonized by the adenomatous polyposis coli (APC) protein supporting β-catenin-degradation. Wnt interplays wi
Google Scholar | PubMed | PMC | DOI
Ion channel Piezo1 induces ferroptosis of trabecular meshwork cells: a novel observation in the pathogenesis in primary open angle glaucoma.
Cell type: general | Technique: general | Disease/area: general | American journal of physiology. Cell physiology (2024)
This study aims to elucidate the role of Piezo1, a mechanosensitive molecule, in trabecular meshwork cells (TMCs) in the context of primary open angle glaucoma (POAG), a leading cause of irreversible visual impairment. Dysfunction of the trabecular meshwork (T
Google Scholar | PubMed | PMC | DOI
GCN2-SLC7A11 axis coordinates autophagy, cell cycle and apoptosis and regulates cell growth in retinoblastoma upon arginine deprivation.
Cell type: cell line | Technique: general | Disease/area: general | Cancer & metabolism (2024)
Arginine deprivation was previously shown to inhibit retinoblastoma cell proliferation and induce cell death in vitro. However, the mechanisms by which retinoblastoma cells respond to arginine deprivation remain to be elucidated. The human-derived retinoblasto
Google Scholar | PubMed | PMC | DOI
Bioprinted High-Cell-Density Laminar Scaffolds Stimulate Extracellular Matrix Production in Osteochondral Co-Cultures.
Cell type: T cell, osteoblast | Technique: general | Disease/area: general | International journal of molecular sciences (2024)
Many tissues have a laminar structure, but there are limited technologies for establishing laminar co-cultures for in vitro testing. Here, we demonstrate that collagen-alginate-fibrin (CAF) hydrogel scaffolds produced using the reactive jet impingement bioprin
Google Scholar | PubMed | PMC | DOI
The GTPase RAB6 is required for stem cell maintenance and cell migration in the gut epithelium.
Cell type: epithelial | Technique: knockout | Disease/area: general | Development (Cambridge, England) (2024)
Intestinal epithelial cells, which are instrumental in nutrient absorption, fluid regulation, and pathogen defense, undergo continuous proliferation and differentiation within the intestinal crypts, migrating towards the luminal surface where they are eventual
Google Scholar | PubMed | PMC | DOI
(Title unavailable)
Cell type: general | Technique: general | Disease/area: general | Molecular therapy. Methods & clinical development (2024)
We developed an
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CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment.
Cell type: endothelial | Technique: general | Disease/area: general | Arteriosclerosis, thrombosis, and vascular biology (2024)
Endothelial cell (EC)-pericyte interactions are known to remodel in response to hemodynamic forces; yet there is a lack of mechanistic understanding of the signaling pathways that underlie these events. Here, we have identified a novel signaling network regula
Google Scholar | PubMed | PMC | DOI
Angiogenesis is limited by LIC1-mediated lysosomal trafficking.
Cell type: endothelial | Technique: general | Disease/area: general | Angiogenesis (2024)
Dynein cytoplasmic 1 light intermediate chain 1 (LIC1, DYNC1LI1) is a core subunit of the dynein motor complex. The LIC1 subunit also interacts with various cargo adaptors to regulate Rab-mediated endosomal recycling and lysosomal degradation. Defects in this
Google Scholar | PubMed | PMC | DOI
Search for putative gene regulatory motifs in CAHS3, linked to anhydrobiosis in a tardigrade Ramazzottius varieornatus, in vivo and in silico.
Cell type: general | Technique: general | Disease/area: general | Genes to cells : devoted to molecular & cellular mechanisms (2024)
Tardigrades possess the ability to enter an almost completely dehydrated state, anhydrobiosis. The CAHS (cytosolic abundant heat-soluble) protein family has been identified as one of the anhydrobiosis-related proteins. In particular, CAHS3 protein from an anhy
Google Scholar | PubMed | PMC | DOI
Novel Therapeutic Effects of
Cell type: general | Technique: general | Disease/area: general | Pharmaceuticals (Basel, Switzerland) (2024)
Natural products with high antioxidant activity are considered as innovative prevention strategies to effectively prevent age-related macular degeneration (AMD) in the early stage because the generation of reactive oxygen species (ROS) leading to the developme
Google Scholar | PubMed | PMC | DOI
Protocol to characterize mouse intestinal epithelial cell lineage using Opal multiplex immunofluorescence.
Cell type: cell line, epithelial | Technique: EdU proliferation, GFP, immunofluorescence | Disease/area: general | STAR protocols (2024)
Applying Opal multiplex immunofluorescence (OMI) to characterize intestinal tissues of genetically engineered mouse models provides an excellent tool for studying complex processes. However, detecting appropriate signals from multiple target molecules is chall
Google Scholar | PubMed | PMC | DOI
Comparison of Muscle Fiber and Meat Quality Characteristics of Beef Strip Loin, Tenderloin, and Round Cuts among Jeju Black Cattle, Hanwoo, and Their Crossbreeds.
Cell type: general | Technique: general | Disease/area: general | Food science of animal resources (2024)
This study assessed and compared meat quality and fiber characteristics of
Google Scholar | PubMed | PMC | DOI
P-NADs:
Cell type: general | Technique: general | Disease/area: general | Heliyon (2024)
Targeted protein degradation (TPD) allows cells to maintain a functional proteome and to rapidly adapt to changing conditions. Methods that repurpose TPD for the deactivation of specific proteins have demonstrated significant potential in therapeutic and resea
Google Scholar | PubMed | PMC | DOI
Three-Dimensional Cell Culture Micro-CT Visualization within Collagen Scaffolds in an Aqueous Environment.
Cell type: general | Technique: general | Disease/area: general | Cells (2024)
Among all of the materials used in tissue engineering in order to develop bioequivalents, collagen shows to be the most promising due to its superb biocompatibility and biodegradability, thus becoming one of the most widely used materials for scaffold producti
Google Scholar | PubMed | PMC | DOI
A Study on the Effect of Quaternization of Polyene Antibiotics' Structures on Their Activity, Toxicity, and Impact on Membrane Models.
Cell type: general | Technique: general | Disease/area: general | Antibiotics (Basel, Switzerland) (2024)
Polyene antibiotics have been used in antifungal therapy since the mid-twentieth century. They are highly valued for their broad spectrum of activity and the rarity of pathogen resistance to their action. However, their use in the treatment of systemic mycoses
Google Scholar | PubMed | PMC | DOI
Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis.
Cell type: general | Technique: general | Disease/area: general | JACS Au (2024)
Microbial metabolic engineering provides a feasible approach to sustainably produce advanced biofuels and biochemicals from renewable feedstocks. Methanol is an ideal feedstock since it can be massively produced from CO
Google Scholar | PubMed | PMC | DOI
Exploiting residual cocoa biomass to extract advanced materials as building blocks for manufacturing nanoparticles aimed at alleviating formation-induced oxidative stress on human dermal fibroblasts.
Cell type: fibroblast | Technique: general | Disease/area: general | Nanoscale advances (2024)
The global adoption of by-product valorisation processes aligns with the circular economy framework, ensuring sustainability in the agricultural sector. In cocoa production, residual biomass can offer the opportunity to extract advanced materials, contributing
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Membrane vectorial lipidomic features of coral host cells' plasma membrane and lipid profiles of their endosymbionts Cladocopium.
Cell type: T cell | Technique: general | Disease/area: general | Communications biology (2024)
The symbiotic relationships between coral animal host and autotrophic dinoflagellates are based on the mutual exchange and tight control of nutritional inputs supporting successful growth. The corals Sinularia heterospiculata and Acropora aspera were cultivate
Google Scholar | PubMed | PMC | DOI
Single-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing.
Cell type: HEK293 | Technique: CRISPR | Disease/area: general | Nature structural & molecular biology (2024)
The development of precise RNA-editing tools is essential for the advancement of RNA therapeutics. CRISPR (clustered regularly interspaced short palindromic repeats) PspCas13b is a programmable RNA nuclease predicted to offer superior specificity because of it
Google Scholar | PubMed | PMC | DOI
GDF-15 Attenuates the Epithelium-Mesenchymal Transition and Alleviates TGFβ2-Induced Lens Opacity.
Cell type: epithelial | Technique: EdU proliferation, western blot | Disease/area: general | Translational vision science & technology (2024)
We sought to evaluate the efficacy of growth differentiation factor (GDF)-15 treatment for suppressing epithelial-mesenchymal transition (EMT) and alleviating transforming growth factor β2 (TGFβ2)-induced lens opacity. To test whether GDF-15 is a molecule that
Google Scholar | PubMed | PMC | DOI
Deep learning based characterization of human organoids using optical coherence tomography.
Cell type: iPSC | Technique: general | Disease/area: general | Biomedical optics express (2024)
Organoids, derived from human induced pluripotent stem cells (hiPSCs), are intricate three-dimensional
Google Scholar | PubMed | PMC | DOI
Genome variation in the Batrachochytrium pathogens of amphibians.
Cell type: general | Technique: general | Disease/area: general | PLoS pathogens (2024)
(Abstract unavailable)
Google Scholar | PubMed | PMC | DOI
Assessment of Ingested Micro- and Nanoplastic (MNP)-Mediated Genotoxicity in an In Vitro Model of the Small Intestinal Epithelium (SIE).
Cell type: general | Technique: general | Disease/area: general | Nanomaterials (Basel, Switzerland) (2024)
Micro- and nanoplastics (MNPs) have become ubiquitous contaminants of water and foods, resulting in high levels of human ingestion exposure. MNPs have been found in human blood and multiple tissues, suggesting that they are readily absorbed by the gastrointest
Google Scholar | PubMed | PMC | DOI
Generation of an Alagille Syndrome (ALGS) patient-derived induced pluripotent stem cell line (TRNDi036-A) carrying a heterozygous mutation (p.Cys693*) in the JAG1 gene.
Cell type: cell line, iPSC | Technique: general | Disease/area: general | Stem cell research (2024)
Alagille syndrome (ALGS) is an autosomal dominant, multisystemic disorder due to haploinsufficiency in JAG1 or less frequently, mutations in NOTCH2. The disease has been difficult to diagnose and treat due to variable expression. The generation of this iPSC li
Google Scholar | PubMed | PMC | DOI
Alternative splicing controls teneurin-3 compact dimer formation for neuronal recognition.
Cell type: neuron | Technique: general | Disease/area: general | Nature communications (2024)
Neuronal network formation is facilitated by recognition between synaptic cell adhesion molecules at the cell surface. Alternative splicing of cell adhesion molecules provides additional specificity in forming neuronal connections. For the teneurin family of c
Google Scholar | PubMed | PMC | DOI
Saturated Iso-Type Fatty Acids from the Marine Bacterium
Cell type: general | Technique: general | Disease/area: general | Pharmaceuticals (Basel, Switzerland) (2024)
Chagas disease is a Neglected Tropical Disease with limited and ineffective therapy. In a search for new anti-trypanosomal compounds, we investigated the potential of the metabolites from the bacteria living in the corals and sediments of the southeastern Braz
Google Scholar | PubMed | PMC | DOI
Leflunomide Treatment Does Not Protect Neural Cells following Oxygen-Glucose Deprivation (OGD) In Vitro.
Cell type: T cell | Technique: general | Disease/area: general | Cells (2024)
Neonatal hypoxia-ischemia (HI) affects 2-3 per 1000 live births in developed countries and up to 26 per 1000 live births in developing countries. It is estimated that of the 750,000 infants experiencing a hypoxic-ischemic event during birth per year, more than
Google Scholar | PubMed | PMC | DOI
Age-dependent loss of Crls1 causes myopathy and skeletal muscle regeneration failure.
Cell type: myoblast | Technique: EdU proliferation | Disease/area: general | Experimental & molecular medicine (2024)
Skeletal muscle aging results in the gradual suppression of myogenesis, leading to muscle mass loss. However, the specific role of cardiolipin in myogenesis has not been determined. This study investigated the crucial role of mitochondrial cardiolipin and card
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Eupatolide, isolated from
Cell type: T cell | Technique: general | Disease/area: general | Heliyon (2024)
Ubiquitination of RIPK1 plays an essential role in the recruitment of the IKK complex, an upstream component of pro-survival NF-κB. It also limits TNF-induced programmed cell death by inhibiting the spatial transition from TNFR1-associated complex-I to RIPK1-d
Google Scholar | PubMed | PMC | DOI
Metformin alleviates junctional epithelium senescence via the AMPK/SIRT1/autophagy pathway in periodontitis induced by hyperglycemia.
Cell type: cell line | Technique: EdU proliferation, immunohistochemistry, western blot | Disease/area: general | Heliyon (2024)
The junctional epithelium (JE) serves a crucial protective role in the periodontium. High glucose-related aging results in accelerated barrier dysfunction of the gingival epithelium, which may be associated with diabetic periodontitis. Metformin, an oral hypog
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The ventral hippocampus is activated in olfactory but not auditory threat memory.
Cell type: general | Technique: general | Disease/area: general | Frontiers in neural circuits (2024)
Hippocampal networks required for associative memory formation are involved in cue- and context-dependent threat conditioning. The hippocampus is functionally heterogeneous at its dorsal and ventral poles, and recent investigations have focused on the specific
Google Scholar | PubMed | PMC | DOI
Effect of Crude Polysaccharides from
Cell type: general | Technique: general | Disease/area: general | Foods (Basel, Switzerland) (2024)
(Abstract unavailable)
Google Scholar | PubMed | PMC | DOI
Precise control of microfluidic flow conditions is critical for harnessing the in vitro transfection capability of pDNA-loaded lipid-Eudragit nanoparticles.
Cell type: HEK293, cell line | Technique: transfection | Disease/area: general | Drug delivery and translational research (2024)
Microfluidics is widely regarded as a leading technology for industrial-scale manufacture of multicomponent, gene-based nanomedicines in a reproducible manner. Yet, very few investigations detail the impact of flow conditions on the biological performance of t
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In vitro proliferation of Mytilus edulis male germ cell progenitors.
Cell type: T cell, epithelial | Technique: EdU proliferation | Disease/area: general | PloS one (2024)
Our understanding of basic cellular processes has mostly been provided by mammalian cell culture, and by some non-mammalian vertebrate and few invertebrate cell culture models. Developing reliable culture conditions for non-model organisms is essential to allo
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Retinoic acid signaling regulates spatiotemporal specification of human green and red cones.
Cell type: general | Technique: general | Disease/area: general | PLoS biology (2024)
Trichromacy is unique to primates among placental mammals, enabled by blue (short/S), green (medium/M), and red (long/L) cones. In humans, great apes, and Old World monkeys, cones make a poorly understood choice between M and L cone subtype fates. To determine
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A hybridized mechano-electroporation technique for efficient immune cell engineering.
Cell type: general | Technique: general | Disease/area: general | Journal of advanced research (2024)
New abstract created by yokesh. Its is unstructured paragraph.
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Assessing MAPPs assay as a tool to predict the immunogenicity potential of protein therapeutics.
Cell type: T cell | Technique: general | Disease/area: general | Life science alliance (2024)
MHC-II-associated peptide proteomics (MAPPs) is a mass spectrometry-based (MS) method to identify naturally presented MHC-II-associated peptides that could elicit CD4+T cell activation. MAPPs assay is considered one of the assays that better characterize the s
Google Scholar | PubMed | PMC | DOI
Exploring the Bioactive Potential of
Cell type: general | Technique: general | Disease/area: general | Foods (Basel, Switzerland) (2023)
(Abstract unavailable)
Google Scholar | PubMed | PMC | DOI
Sibjotang Protects against Cardiac Hypertrophy In Vitro and In Vivo.
Cell type: general | Technique: general | Disease/area: general | Life (Basel, Switzerland) (2023)
Cardiac hypertrophy is developed by various diseases such as myocardial infarction, valve diseases, hypertension, and aortic stenosis. Sibjotang (, Shizaotang, SJT), a classic formula in Korean traditional medicine, has been shown to modulate the equilibrium o
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Effect of GelMA Hydrogel Properties on Long-Term Encapsulation and Myogenic Differentiation of C
Cell type: general | Technique: general | Disease/area: general | Gels (Basel, Switzerland) (2023)
Skeletal muscle regeneration and engineering hold great promise for the treatment of various muscle-related pathologies and injuries. This research explores the use of gelatin methacrylate (GelMA) hydrogels as a critical component for encapsulating cellular sp
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Investigating the Impact of Mechanical Properties and Cell-Collagen Interaction on NIH3T3 Function: A Comparative Study on Different Substrates and Culture Environments.
Cell type: T cell, fibroblast | Technique: general | Disease/area: general | Gels (Basel, Switzerland) (2023)
This study investigates the intricate dynamics of matrix stiffness, substrate composition, and cell-cell interactions and elucidates their cumulative effects on fibroblast behavior in different culture contexts. Three primary substrate types were examined: non
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Risk Assessment of Industrial Microbes Using a Terrestrial Mesocosm Platform.
Cell type: general | Technique: general | Disease/area: general | Microbial ecology (2023)
Industrial microbes and bio-derived products have emerged as an integral component of the bioeconomy, with an array of agricultural, bioenergy, and biomedical applications. However, the rapid development of microbial biotechnology raises concerns related to en
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In vivo ameliorative effects of vitamin E against hydralazine-induced lupus.
Cell type: general | Technique: general | Disease/area: general | Lupus science & medicine (2023)
In this study, we investigated the in vivo ameliorative effects of vitamin E in a hydralazine-induced lupus model, which closely resembles SLE in humans. We aim to shed light on its potential as a therapeutic agent for managing SLE. Forty BALB/c mice were used
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Fungal drops: a novel approach for macro- and microscopic analyses of fungal mycelial growth.
Cell type: general | Technique: general | Disease/area: general | microLife (2023)
This study presents an inexpensive approach for the macro- and microscopic observation of fungal mycelial growth. The 'fungal drops' method allows to investigate the development of a mycelial network in filamentous microorganisms at the colony and hy
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