EVOS M5000 in Cell Biology & Signalling: Independent Studies — Wave 3 Part 2
Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.
EVOS M5000IndependentWave 3
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FANCD2-dependent mitotic DNA synthesis relies on PCNA K164 ubiquitination.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Ubiquitination of proliferating cell nuclear antigen (PCNA) at lysine 164 (K164) activates DNA damage tolerance pathways. Currently, we lack a comprehensive understanding of how PCNA K164 ubiquitination promotes genome stability. To evaluate this, we generated stable cell lines expressing PCNA
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Effects of crude protein and non-essential amino acids on growth performance, blood profile, and intestinal health of weaned piglets.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
This study investigated the effect of crude protein (CP) and non-essential amino acid (NEAA) supplementation on the growth performance, blood profile, intestinal morphology, mRNA relative abundance of inflammatory and antioxidant markers, and tight junction proteins in piglets over the first 2 weeks after weaning. Ninety 21-day-old piglets (7.55 ± 0.72 kg) were assigned in a ra
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Effect of GelMA Hydrogel Properties on Long-Term Encapsulation and Myogenic Differentiation of C
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Skeletal muscle regeneration and engineering hold great promise for the treatment of various muscle-related pathologies and injuries. This research explores the use of gelatin methacrylate (GelMA) hydrogels as a critical component for encapsulating cellular spheroids in the context of muscle tissue engineering and regenerative applications. The preparation of GelMA hydrogels at
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Irisin Enhances Mitochondrial Function in Osteoclast Progenitors during Differentiation.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Irisin is a myokine released from muscle during exercise with distinct signaling effects on tissues throughout the body, including an influence on skeletal remodeling. Our previous work has shown that irisin stimulates resorption, a key first step in bone remodeling, by enhancing osteoclastogenesis. The present study further investigates the action of irisin on the metabolic fu
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Recombinant human secretory leukocyte protease inhibitor (rhSLPI) coated titanium enhanced human osteoblast adhesion and differentiation.
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Osseointegration is vital to success in orthopedic and dental reconstructions with implanted materials. The bone matrix or cells-particularly osteoblasts-are required to achieve functional contact on the implant surface. Osteoblast induction is therefore essential for osteogenesis to occur. Enhancement of osteoblast adhesion, proliferation, and differentiation, particularly by
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Streamlined Methods for Processing and Cryopreservation of Cell Therapy Products Using Automated Systems.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Streamlined procedures for processing and cryopreservation of cell therapies using good laboratory practices are integral to biomanufacturing process development and clinical applications. The protocol herein begins with the preparation of human cell types cultured as adherent (i.e., mesenchymal stromal cells, MSCs) or suspension cells (i.e., peripheral blood mononuclear cells,
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Intermittent short-duration reoxygenation relieves high-altitude pulmonary hypertension via NOX4/H2O2/PPAR-γ axis.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
High-altitude pulmonary hypertension (HAPH) is a severe and progressive disease that can lead to right heart failure. Intermittent short-duration reoxygenation at high altitude is effective in alleviating HAPH; however, the underlying mechanisms are unclear. In the present study, a simulated 5,000-m hypoxia rat model and hypoxic cultured pulmonary artery smooth muscle cells (PA
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Precise control of microfluidic flow conditions is critical for harnessing the in vitro transfection capability of pDNA-loaded lipid-Eudragit nanoparticles.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Microfluidics is widely regarded as a leading technology for industrial-scale manufacture of multicomponent, gene-based nanomedicines in a reproducible manner. Yet, very few investigations detail the impact of flow conditions on the biological performance of the product, particularly biocompatibility and therapeutic efficiency. Herein, this study investigated the engineering of
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Effect of Crude Polysaccharides from
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
<i>Ecklonia cava</i>, a brown seaweed native to the East Asian coast, is known for its unique composition, including polysaccharides, polyphenols, and phlorotannins. Fucoidan is a sulfated polysaccharide widely used as a functional ingredient in foods. This study obtained crude polysaccharides (ECC_CPS) from <i>E. cava</i> celluclast enzymatic hydrolysate using ethanol precipit
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5-HT
Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Vascular and mitochondrial dysfunction are well-established consequences of multiple central nervous system (CNS) disorders, including neurodegenerative diseases and traumatic injuries. We previously reported that 5-hydroxytryptamine 1F receptor (5-HT Lasmiditan is a selective 5-HT Lasmiditan treatment increased the maximal oxygen consumption rate, mitochondrial proteins and mi
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Mitochondrial Transplantation Therapy Ameliorates Muscular Dystrophy in
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Duchenne muscular dystrophy is caused by loss of the dystrophin protein. This pathology is accompanied by mitochondrial dysfunction contributing to muscle fiber instability. It is known that mitochondria-targeted in vivo therapy mitigates pathology and improves the quality of life of model animals. In the present work, we applied mitochondrial transplantation therapy (MTT) to c
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Age-dependent loss of Crls1 causes myopathy and skeletal muscle regeneration failure.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Skeletal muscle aging results in the gradual suppression of myogenesis, leading to muscle mass loss. However, the specific role of cardiolipin in myogenesis has not been determined. This study investigated the crucial role of mitochondrial cardiolipin and cardiolipin synthase 1 (Crls1) in age-related muscle deterioration and myogenesis. Our findings demonstrated that cardiolipi
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An adapted protocol to derive microglia from stem cells and its application in the study of CSF1R-related disorders.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Induced pluripotent stem cell-derived microglia (iMGL) represent an excellent tool in studying microglial function in health and disease. Yet, since differentiation and survival of iMGL are highly reliant on colony-stimulating factor 1 receptor (CSF1R) signaling, it is difficult to use iMGL to study microglial dysfunction associated with pathogenic defects in CSF1R. Serial modi
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Microfluidic Fabricated Liposomes for Nutlin-3a Ocular Delivery as Potential Candidate for Proliferative Vitreoretinal Diseases Treatment.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Proliferative vitreoretinal diseases (PVDs) represent a heterogeneous group of pathologies characterized by the presence of retinal proliferative membranes, in whose development retinal pigment epithelium (RPE) is deeply involved. As the only effective treatment for PVDs at present is surgery, we aimed to investigate the potential therapeutic activity of Nutlin-3a, a small non-
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ASPSCR1::TFE3 Drives Alveolar Soft Part Sarcoma by Inducing Targetable Transcriptional Programs.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Alveolar soft part sarcoma (ASPS) is a rare mesenchymal malignancy driven by the ASPSCR1::TFE3 fusion. A better understanding of the mechanisms by which this oncogenic transcriptional regulator drives cancer growth is needed to help identify potential therapeutic targets. In this study, we characterized the transcriptional and chromatin landscapes of ASPS tumors and preclinical
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Saturated Iso-Type Fatty Acids from the Marine Bacterium
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Chagas disease is a Neglected Tropical Disease with limited and ineffective therapy. In a search for new anti-trypanosomal compounds, we investigated the potential of the metabolites from the bacteria living in the corals and sediments of the southeastern Brazilian coast. Three corals,
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Generation of an Alagille Syndrome (ALGS) patient-derived induced pluripotent stem cell line (TRNDi036-A) carrying a heterozygous mutation (p.Cys693*) in the JAG1 gene.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Alagille syndrome (ALGS) is an autosomal dominant, multisystemic disorder due to haploinsufficiency in JAG1 or less frequently, mutations in NOTCH2. The disease has been difficult to diagnose and treat due to variable expression. The generation of this iPSC line (TRNDi036-A) carrying a heterozygous mutation (p.Cys693*) in the JAG1 gene provides a means of studying the disease a
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SGLT2 inhibitor promotes mitochondrial dysfunction and ER-phagy in colorectal cancer cells.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Sodium-glucose transporter 2 (SGLT2) inhibitors (iSGLT2) are approved medications for type 2 diabetes. Recent studies indicate that iSGLT2 inhibit the growth of some cancer cells. However, the mechanism(s) remains to be fully elucidated. The SGLT2 levels were determined in normal colon CCD 841 CoN and, HCT 116, HT-29, SW480 and LoVo colorectal cancer (CRC) cell lines by quantit
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Enhanced chondrogenic potential in GelMA-based 3D cartilage model via Wnt3a surface immobilization.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Cartilage tissue engineering aims to develop functional substitutes for treating cartilage defects and osteoarthritis. Traditional two-dimensional (2D) cell culture systems lack the complexity of native cartilage, leading to the development of 3D regenerative cartilage models. In this study, we developed a 3D model using Gelatin Methacryloyl (GelMA)-based hydrogels seeded with
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An insight into carcinogenic activity and molecular mechanisms of Bis(2-ethylhexyl) phthalate.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Di(ethylhexyl) phthalate (DEHP) is a ubiquitous environmental contaminant to which humans are exposed via multiple routes. Human health risk assessments for this substance have recently been updated, focusing on reproductive toxicity, including DEHP, in the list of chemicals classified as carcinogenic, mutagenic, or toxic to reproduction (CMR). Moreover, DEHP has also been defi
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Inhibition of miR-10b treats metastatic breast cancer by targeting stem cell-like properties.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Despite advances in breast cancer screening and treatment, prognosis for metastatic disease remains dismal at 30% five-year survival. This is due, in large, to the failure of current therapeutics to target properties unique to metastatic cells. One of the drivers of metastasis is miR-10b, a small noncoding RNA implicated in cancer cell invasion, migration, viability, and prolif
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In Vitro Studies to Evaluate the Intestinal Permeation of an Ursodeoxycholic Acid-Conjugated Oligonucleotide for Duchenne Muscular Dystrophy Treatment.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Delivery represents a major hurdle to the clinical advancement of oligonucleotide therapeutics for the treatment of disorders such as Duchenne muscular dystrophy (DMD). In this preliminary study, we explored the ability of 2'-
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Precision therapy targeting CAMK2 to overcome resistance to EGFR inhibitors in FAT1 -mutated oral squamous cell carcinoma.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Oral squamous cell carcinoma (OSCC) is a prevalent type of cancer with a high mortality rate in its late stages. One of the major challenges in OSCC treatment is the resistance to epidermal growth factor receptor (EGFR) inhibitors. Therefore, it is imperative to elucidate the mechanism underlying drug resistance and develop appropriate precision therapy strategies to enhance cl
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Comparison of Muscle Fiber and Meat Quality Characteristics of Beef Strip Loin, Tenderloin, and Round Cuts among Jeju Black Cattle, Hanwoo, and Their Crossbreeds.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
This study assessed and compared meat quality and fiber characteristics of
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Decoding Cold Therapy Mechanisms of Enhanced Bone Repair through Sensory Receptors and Molecular Pathways.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Applying cold to a bone injury can aid healing, though its mechanisms are complex. This study investigates how cold therapy impacts bone repair to optimize healing. Cold was applied to a rodent bone model, with the physiological responses analyzed. Vasoconstriction was mediated by an increase in the transient receptor protein channels (TRPs), transient receptor potential ankyri
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Reduction of Mitochondrial Calcium Overload via MKT077-Induced Inhibition of Glucose-Regulated Protein 75 Alleviates Skeletal Muscle Pathology in Dystrophin-Deficient
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Duchenne muscular dystrophy is secondarily accompanied by Ca
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ZIC2 and ZIC3 promote SWI/SNF recruitment to safeguard progression towards human primed pluripotency.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The primed epiblast acts as a transitional stage between the relatively homogeneous naïve epiblast and the gastrulating embryo. Its formation entails coordinated changes in regulatory circuits driven by transcription factors and epigenetic modifications. Using a multi-omic approach in human embryonic stem cell models across the spectrum of peri-implantation development, we demo
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<i>In vitro</i> and <i>in vivo</i> expansion of CD33/HBG promoter-edited HSPCs with Mylotarg.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
We developed an
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The GTPase RAB6 is required for stem cell maintenance and cell migration in the gut epithelium.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Intestinal epithelial cells, which are instrumental in nutrient absorption, fluid regulation, and pathogen defense, undergo continuous proliferation and differentiation within the intestinal crypts, migrating towards the luminal surface where they are eventually shed. RAB GTPases are key regulators of intracellular vesicular trafficking and are involved in various cellular proc
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GCN2-SLC7A11 axis coordinates autophagy, cell cycle and apoptosis and regulates cell growth in retinoblastoma upon arginine deprivation.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Arginine deprivation was previously shown to inhibit retinoblastoma cell proliferation and induce cell death in vitro. However, the mechanisms by which retinoblastoma cells respond to arginine deprivation remain to be elucidated. The human-derived retinoblastoma cell lines Y79 and WERI-Rb-1 were subjected to arginine depletion, and the effects on inhibiting cell growth and surv
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Ion channel Piezo1 induces ferroptosis of trabecular meshwork cells: a novel observation in the pathogenesis in primary open angle glaucoma.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
This study aims to elucidate the role of Piezo1, a mechanosensitive molecule, in trabecular meshwork cells (TMCs) in the context of primary open angle glaucoma (POAG), a leading cause of irreversible visual impairment. Dysfunction of the trabecular meshwork (TM) is a key factor in the elevated intraocular pressure (IOP) observed in POAG, yet the specific mechanisms leading to T
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PTPN1/2 inhibition promotes muscle stem cell differentiation in Duchenne muscular dystrophy.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Duchenne muscular dystrophy (DMD) is a lethal disease caused by mutations in the
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Role of Peroxisome Proliferator-Activated Receptor α-Dependent Mitochondrial Metabolism in Ovarian Cancer Stem Cells.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Peroxisome proliferator-activated receptors (PPARs), including PPAR-α, PPAR-β/δ, and PPAR-γ, are involved in various cellular responses, including metabolism and cell proliferation. Increasing evidence suggests that PPARs are closely associated with tumorigenesis and metastasis. However, the exact role of PPARs in energy metabolism and cancer stem cell (CSC) proliferation remai
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Neuromodulation Improves Stress Urinary Incontinence-Like Deficits in Female Rabbits.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
<i>Objective:</i> Stress urinary incontinence (SUI) affects a third of the female population and is characterized by involuntary urine leakage during abdominal efforts such as sneezing, laughing, or coughing. Acute neuromodulation of the bulbospongiosus nerve (BsN) was shown to increase bladder efficiency in aged and multiparous rabbits. This study investigates the efficacy of
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Morphoregulatory ADD3 underlies glioblastoma growth and formation of tumor-tumor connections.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Glioblastoma is a major unmet clinical need characterized by striking inter- and intra-tumoral heterogeneity and a population of glioblastoma stem cells (GSCs), conferring aggressiveness and therapy resistance. GSCs communicate through a network of tumor-tumor connections (TTCs), including nanotubes and microtubes, promoting tumor progression. However, very little is known abou
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R-loop formation contributes to mTORC1 activation-dependent DNA replication stress induced by p53 deficiency.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
DNA replication stress is a significant contributor to spontaneous DNA damage and genome instability. While the impact of p53 deficiency on increasing DNA replication stress is known, the specific molecular mechanism underlying this phenomenon remains poorly understood. This study explores how p53 deficiency induces DNA replication stress by activating mTORC1 through R-loop for
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Impact of Drying on Phytonutritional Compounds, In Vitro Antioxidant Activity and Cytotoxicity of Spiny Saltbush (
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The Spiny saltbush (
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Evaluation of the Efficacy of a Full-Spectrum Low-THC
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Evidence has accumulated that
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Pyrvinium Pamoate Alone and With Gemcitabine Exhibits Anti-Pancreatic Cancer Activity in 2D and 3D Cell Culture Models.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Pancreatic cancer is an intractable disease with the worst prognosis of all common cancers. The treatment regimens currently used for pancreatic cancer do not significantly impact patient survival, and therefore, effective treatment strategies are urgently needed. Drug repurposing, which identifies new indications for existing and approved drugs, has proven to be a desirable ap
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Generation of induced pluripotent stem cell lines TRNDi037-A and TRNDi038-A from two patients with Alagille syndrome carrying heterozygous JAG1 mutations.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Human induced pluripotent stem cell (iPSC) lines TRNDi037-A and TRNDi038-A were generated from the lymphoblastoid cell lines (LCL) of two patients with different heterozygous JAG1 variants resulting in Alagille syndrome (ALGS). ALGS is a rare genetic disease of haploinsufficiency that affects the formation of the bile duct, in addition to other symptoms. These ALGS iPSC lines c
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iPSC-derived human sensory neurons reveal a subset of TRPV1 antagonists as anti-pruritic compounds.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Signaling interplay between the histamine 1 receptor (H1R) and transient receptor potential cation channel subfamily V member 1 (TRPV1) in mediating histaminergic itch has been well-established in mammalian models, but whether this is conserved in humans remains to be confirmed due to the difficulties in obtaining human sensory neurons (SNs) for experimentation. Additionally, p
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Mitochondrial epigenetics brings new perspectives on doubly uniparental inheritance in bivalves.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Mitochondrial epigenetics, particularly mtDNA methylation, is a flourishing field of research. MtDNA methylation appears to play multiple roles, including regulating mitochondrial transcription, cell metabolism and mitochondrial inheritance. In animals, bivalves with doubly uniparental inheritance (DUI) of mitochondria are the exception to the rule of maternal mitochondrial inh
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Demystifying the functions of the mitochondrial ORFan proteins in bivalves with doubly uniparental inheritance.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Strict maternal inheritance of mitochondria is known to be the rule in animals, but over 100 species across six orders of bivalves possess doubly uniparental inheritance (DUI) of mitochondria. Under DUI, two distinctive sex-specific mitogenomes coexist. In marine and freshwater mussels, each mitogenome has an additional protein-coding gene, called female- and male-specific open
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Bile acid sequestrant inhibits gluconeogenesis via inducing hepatic cysteine dioxygenase type 1 to reduce cysteine availability.
Cell type: Hepatocyte | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Bile acid sequestrants such as cholestyramine (ChTM) are gut-restricted bile acid-binding resins that block intestine bile acid absorption and attenuate hepatic bile acid signaling. Bile acid sequestrants induce hepatic bile acid synthesis to promote cholesterol catabolism and are cholesterol-lowering drugs. Bile acid sequestrants also reduce blood glucose in clinical trials an
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BRCA1 is involved in sustaining rapid antler growth possibly via balancing of the p53/endoplasmic reticulum stress signaling pathway.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Regeneration is the preferred approach to restore the structure and function after tissue damage. Rapid proliferation of cells over the site of damage is integral to the process of regeneration. However, even subtle mutations in proliferating cells may cause detrimental effects by eliciting abnormal differentiation. Interestingly deer antlers, arguably the fastest regenerating
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FAM43A coordinates mtDNA replication and mitochondrial biogenesis in response to mtDNA depletion.
Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Mitochondrial retrograde signaling (MRS) pathways relay the functional status of mitochondria to elicit homeostatic or adaptive changes in nuclear gene expression. Budding yeast have "intergenomic signaling" pathways that sense the amount of mitochondrial DNA (mtDNA) independently of oxidative phosphorylation (OXPHOS), the primary function of genes encoded by mtDNA. However, MR
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Circular RNA Telomerase Reverses Endothelial Senescence in Progeria.
Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Telomeres shorten with each cell division, acting as a chronometer of cell age. The enzyme telomerase, primarily active in stem cells, reverses telomere erosion. We have previously observed that transient transfection with human TERT mRNA extends telomeres and mitigates hallmarks of senescence in replicatively aged human cells or those affected by Hutchinson-Gilford progeroid s
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Nasal irrigation solution is less cytotoxic to nasal epithelial cells than nasal spray.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Recent studies have demonstrated that corticosteroid delivered by nasal irrigation is superior to nasal spray in the treatment of chronic rhinosinusitis patients who have undergone sinus surgery. However, the local cytotoxicity of both delivery methods has not been previously evaluated. In this study we aimed to evaluate the cytotoxicity of corticosteroid prepared nasal irrigat
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Obese Adipose Tissue Extracellular Vesicles Activate Mitochondrial Fatty Acid β-oxidation to Drive Colonic Stemness.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Patients with obesity and mouse models of obesity exhibit abnormalities in intestinal epithelial cells, including enhanced stemness. Adipose tissue (AT) is the largest endocrine organ secreting cytokines, hormones, and extracellular vesicles (EVs). Here, we characterized EV protein cargo from obese and non-obese AT and demonstrate the role of obese adipose-derived EVs in enhanc
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Haem biosynthesis regulates BCAA catabolism and thermogenesis in brown adipose tissue.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The distinctive colour of brown adipose tissue (BAT) is attributed to its high content of haem-rich mitochondria. However, the mechanisms by which BAT regulates intracellular haem levels remain largely unexplored. Here we demonstrate that haem biosynthesis is the primary source of haem in brown adipocytes. Inhibiting haem biosynthesis results in an accumulation of the branched-
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NEDD4 E3 ligase-catalyzed NAMPT ubiquitination and autophagy activation are essential for pyroptosis-independent NAMPT secretion in human monocytes.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
NAMPT is an important intracellular metabolic enzyme (iNAMPT) regulating the NAD
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Allogeneic RPE cell suspension manufactured at scale demonstrating preclinical safety and efficacy led to IND approval.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Cell replacement therapy is a promising therapeutic option for dry age-related macular degeneration (AMD). In this study, we outline our design for scalable manufacture with appropriate quality gates and present in vivo data for establishing preclinical safety and efficacy of an induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium (RPE) product, thus laying t
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RXFP2-positive mesenchymal stem cells in the antlerogenic periosteum contribute to postnatal development of deer antlers.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The postnatal development of secondary sexual characteristics is a highly complex process governed by diverse molecular signals and serves as a key marker of sexual maturity. Deer antlers exemplify such traits, distinguished not only by their unique ability to regenerate annually but also by their initiation in postnatal life. It is well established that the antlerogenic perios
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Sevoflurane: A dual modulator of miR‑211‑5p and mitochondrial apoptosis in glioma therapy.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The present study aimed to investigate how sevoflurane (SEV) regulated the apoptosis of glioma cells through the mitochondrial apoptosis pathway. First, an evaluation was performed on the viability, apoptosis, mitochondrial reactive oxygen species levels, mitochondrial membrane potential and apoptosis and autophagy‑related protein expression of glioma cells according to experim
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l-Carnitine and Acetyl-l-Carnitine Induce Metabolism Alteration and Mitophagy-Related Cell Death in Colorectal Cancer Cells.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
<b>Background/Objectives</b>: Colorectal cancer (CRC) remains one of the most common and deadly malignancies worldwide, driven by metabolic reprogramming and mitochondrial dysfunction, which support tumor growth and progression. Several studies showed that nutrition is a contributing factor in the prevention and management of CRC. In this context, carnitines, amino acid derivat
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