EVOS M5000 in Cell Biology & Signalling: Independent Studies — Wave 3 Part 3
Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.
EVOS M5000IndependentWave 3
Parts: Part 1 | Part 2 | Part 3
← Back to EVOS M5000 Research Index
Effect of CTMP1 gene on pulmonary fibrosis.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Protein kinase B (PKB/AKT) is a very important member of the protein kinase family, playing significant roles in various crucial processes including insulin-signaling, cell survival, growth, and metabolism. The carboxyl-terminal modulator protein 1 (CTMP1) inhibits PKB, primarily by attenuating its phosphorylation. Idiopathic pulmonary fibrosis (IPF) is an irreversible, chronic
Google Scholar | PubMed | PMC | DOI
Structured Light Projection Using Image Guide Fibers for In Situ Photo-biofabrication.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Light-based biofabrication techniques have revolutionized the field of tissue engineering and regenerative medicine. Specifically, the projection of structured light, where the spatial distribution of light is controlled at both macro and microscale, has enabled precise fabrication of complex three dimensional structures with high resolution and speed. However, despite tremendo
Google Scholar | PubMed | PMC | DOI
Extrusion of BMP2+ surface colonocytes promotes stromal remodeling and tissue regeneration.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The colon epithelium frequently incurs damage through toxic influences. Repair is rapid, mediated by cellular plasticity and acquisition of the highly proliferative regenerative state. However, the mechanisms that promote the regenerative state are not well understood. Here, we reveal that upon injury and subsequent inflammatory response, IFN-γ drives widespread epithelial remo
Google Scholar | PubMed | PMC | DOI
Comprehensive secretome profiling and CRISPR screen identifies SFRP1 as a key inhibitor of epidermal progenitor proliferation.
Cell type: Keratinocyte | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Secreted proteins are crucial for the structure and functions of the human epidermis, but the full repertoire of the keratinocyte secretome has not been experimentally defined. In this study, we performed mass spectrometry on conditioned media from primary human keratinocytes, identifying 406 proteins with diverse roles in adhesion, migration, proliferation, proteolysis, signal
Google Scholar | PubMed | PMC | DOI
Posttranscriptional RNA stabilization of telomeric RNAs FRG2, DBE-T, D4Z4 at human 4q35 in response to genotoxic stress and D4Z4 macrosatellite repeat length.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Reduced copy number of the D4Z4 macrosatellite at human chromosome 4q35 is associated with facioscapulohumeral muscular dystrophy (FSHD). A pervasive idea is that chromatin alterations at the 4q35 locus following D4Z4 repeat unit deletion lead to disease via inappropriate expression of nearby genes. Here, we sought to analyze transcription and chromatin characteristics at speci
Google Scholar | PubMed | PMC | DOI
The Mechanism of a Novel Mitochondrial-Targeted Icaritin Derivative in Regulating Apoptosis of BEL-7402 Cells Based on the SIRT3 and CypD-Mediated ROS/p38 MAPK Signaling Pathway.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Tumorigenesis and progression are closely associated with apoptosis and primarily regulated by mitochondria, which are considered major targets for cancer therapy. In this study, twelve novel icaritin (ICT) derivatives were designed and synthesized, four of which were specifically targeted to mitochondria. Biological studies demonstrated that all compounds containing triphenylp
Google Scholar | PubMed | PMC | DOI
Distinct signalling dynamics of BMP4 and BMP9 in brown versus white adipocytes.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Adipocyte dysfunction contributes to lipotoxicity and cardiometabolic diseases. Bone morphogenetic protein 4 (BMP4) is expressed in white adipocytes and remodels white adipose tissue, while liver-derived BMP9, a key circulating BMP, influences adipocyte lipid metabolism. The gene sets regulated by BMP4 and BMP9 signalling in mature adipocytes remain unclear. Here, we directly c
Google Scholar | PubMed | PMC | DOI
MiR-122-5p inhibits the epithelial mesenchymal transition of liver cancer cells by inducing hiPSCs to differentiate into hepatocyte-like cells.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Epithelial-mesenchymal transition (EMT) is closely linked to liver cancer prognosis, invasiveness, and aggressiveness. One promising treatment for liver cancer is cell therapy, where stem cells are stimulated to develop into functional liver cells. This study aimed to investigate the effect of miR-122-5p on the differentiation of human induced pluripotent stem cells (hiPSCs) in
Google Scholar | PubMed | PMC | DOI
Ribosome association inhibits stress-induced gene mRNA localization to stress granules.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The integrated stress response (ISR) is critical for resilience to stress and is implicated in numerous diseases. During the ISR, translation is repressed, stress-induced genes are expressed, and mRNAs condense into stress granules. The relationship between stress granules and stress-induced gene expression is unclear. We measured endogenous stress-induced gene mRNA localizatio
Google Scholar | PubMed | PMC | DOI
Evernic Acid: A Low-Toxic and Selective Alternative to Chemotherapeutic Agents in the Treatment of Ovarian Cancer.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Evernic acid (EA) has emerged as a potential therapeutic agent with its low toxicity and anticancer properties. In this study, the anticancer effect of EA on ovarian cancer cell lines and normal ovarian surface epithelial cells (OSE) was evaluated. The antiproliferative effect of EA was evaluated by xCELLigence Real-Time Cell analysis, colony formation assay, and acridine orang
Google Scholar | PubMed | PMC | DOI
Protective Action of 3,5-Diiodo-L-Thyronine on Cigarette Smoke-Induced Mitochondrial Dysfunction in Human Alveolar Epithelial Cells.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
<b>Background</b>: Cigarette smoke (CS) is a major risk factor for chronic lung conditions. Oxidative stress and mitochondrial dysfunction play a crucial role in CS-induced pulmonary injury. 3,5-Diiodothyronine (T2) affects energy metabolism, having mitochondria as a major target. However, the underlying mechanisms of T2 related to lung diseases are poorly understood. <b>Aims</
Google Scholar | PubMed | PMC | DOI
Harnessing cell size to separate genetically and functionally distinct dental pulp-derived mesenchymal stromal cell subpopulations.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
BACKGROUND: Dental pulp-derived mesenchymal stromal cells (DPMSCs) represent a promising avenue for regenerative medicine. However, the therapeutic potency of DPMSCs is substantially influenced by their functional heterogeneity emerging during ex vivo expansion. Therefore, identifying and selecting the most potent subpopulation of DPMSCs following expansion is key to improving
Google Scholar | PubMed | PMC | DOI
Muscle stem cells in Duchenne muscular dystrophy exhibit molecular impairments and altered cell fate trajectories impacting regenerative capacity.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Satellite cells are muscle-resident stem cells that maintain and repair muscle. Increasing evidence supports the contributing role of satellite cells in Duchenne muscular dystrophy (DMD), a lethal degenerative muscle disease caused by loss of dystrophin. However, whether or not satellite cells exhibit dysfunction due to loss of dystrophin remains unresolved. Here, we used singl
Google Scholar | PubMed | PMC | DOI
Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Lamellipodia are sheet-like protrusions essential for cell migration and endocytosis, but their ultrastructural dynamics remain poorly understood because conventional electron microscopy lacks temporal resolution. Here, we combined optogenetics with cryo-electron tomography (cryo-ET) to visualize the actin cytoskeleton and membrane structures during lamellipodia formation with
Google Scholar | PubMed | PMC | DOI
Signaling Centers Drive Brachyury Dynamics and Lineage Commitment in mESC Aggregates.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Stem cell-based models of embryogenesis have exploded in popularity, resulting in protocols with overlapping use of some reagents and differential use of others. As such, the precise contributions of individual signaling molecules, such as Chiron and BMP4, applied to whole or part of aggregates, and matrices, such as Matrigel, to the development of these models are unknown. Fur
Google Scholar | PubMed | PMC | DOI
Rat Hair Follicle Stem Cell-Derived Exosomes: Isolation, Characterization and Comparative Analysis of Their In Vitro Wound Healing Potential.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Stem cell-derived secretome and exosomes present a promising cell-free strategy for tissue repair and wound healing. This study aimed to isolate and characterize, for the first time, exosomes derived from rat hair follicle stem cells (rHFSCs) and to evaluate their wound-healing potential alongside rHFSC secretome. Exosomes were isolated via ultracentrifugation and characterized
Google Scholar | PubMed | PMC | DOI
Repeated Low-Level Inflammatory Challenge Leads to Alterations in the TNF-CXCL10 Signalling Pathway in Mouse Cerebral Endothelial Cells In Vitro.
Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The mechanism by which chronic systemic inflammation contributes to cerebral endothelial dysfunction and neurological disorders is unclear, although endothelial inflammatory signalling is considered a cornerstone of this process. Here, we have performed transcriptomic analysis of published RNASeq datasets and identified consistent upregulation of the Tumour Necrosis Factor-C-X-
Google Scholar | PubMed | PMC | DOI
Clinical Scale MSC-Derived Extracellular Vesicles Enhance Poststroke Neuroplasticity in Rodents and Non-Human Primates.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Stroke is a leading cause of death and disability. The therapeutic potential of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) has shown considerable promise in rodent models of stroke. However, the therapeutic efficacy and safety of clinical-scale MSC-EVs for ischemic stroke are not well elucidated, especially in non-human primates. We developed a scalable prod
Google Scholar | PubMed | PMC | DOI
BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
<b>Background:</b> Taste disorders in patients with type 2 diabetes mellitus (T2DM) have a negative impact on their quality of life and glycemic control, and treatment options are limited. Buzhong yiqi formula (BZYQF) improves T2DM symptoms but its effects on T2DM-induced taste disorders have not been sufficiently studied. <b>Methods</b>: Molecular docking was utilized to evalu
Google Scholar | PubMed | PMC | DOI
Dual treatment with Val-083 and AZD1775 shows potent anti-tumor activity in diffuse midline glioma models.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
H3K27M diffuse midline gliomas (DMG) are characterized by p53 mutations and hypomethylation of MGMT, a DNA-repair enzyme, leading to resistance towards chemotherapeutic agents such as temozolomide (TMZ). As an alternative, we investigated the efficacy of a functionally different DNA-damaging agent, Val-083, on our DMG models. Val-083 is a blood-brain barrier penetrant DNA targe
Google Scholar | PubMed | PMC | DOI
Bioactive lipid mediator class switching regulates myogenic cell progression and muscle regeneration.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The muscle stem cell niche is well-described as influencing myogenic cell fate decision; however, the intrinsic mechanisms driving muscle stem cell progression during myogenesis are not yet fully elucidated. Here, we demonstrate that bioactive lipid class switching, an auto-regulatory mechanism originally described during the inflammatory process, is conserved during myogenesis
Google Scholar | PubMed | PMC | DOI
Mitochondrial apoptosis induced by MNAT1 in laryngeal squamous cell carcinoma cells reverses drug resistance.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Laryngeal squamous cell carcinoma (LSCC), the predominant histological subtype of laryngeal cancer with a poor diagnosis, requires further exploration of its molecular mechanisms and potential therapeutic targets. The expression of MNAT1 in LSCC was detected by western blotting and IHC. EDU analysis, colony formation assay, scratch assay, transwell assay and flow cytometry were
Google Scholar | PubMed | PMC | DOI
Facile Single-Nanocomposite 4D Bioprinting of Dynamic Hydrogel Constructs with Thickness-Controlled Gradient.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The advent of 4D bioprinting has fueled significant progress in tissue engineering, but it faces major challenges such as limited options of smart bioinks and complexity of designing printing paths, limiting its broader application in tissue engineering. In this study, a smart composite hydrogel is first developed by combining gelatin, gelatin methacryloyl, and MXene (MX/GG), e
Google Scholar | PubMed | PMC | DOI
Odontogenesis-associated phosphoprotein (ODAPH) Promotes Ameloblast adhesion and alkaline phosphatase (ALP) expression via LAMC2/ ITGB6/TGF-β1 signaling pathway.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Recessive hypomineralized amelogenesis imperfecta has been linked to mutations in Odontogenesis-Associated Phosphoprotein (ODAPH). Consistent with human phenotypes, Odaph-null mice exhibit defective enamel mineralization with ameloblast detachment from the enamel surface. To elucidate the mechanistic basis, we investigated ODAPH's role in ameloblast adhesion and mineralization
Google Scholar | PubMed | PMC | DOI
Involvement of miR-205-5p in mediating the development of nodular thyroid disease associated with coal worker's pneumoconiosis via pulmonary extracellular vesicles.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Coal worker's pneumoconiosis (CWP) is an occupational disease, and the mechanisms underlying the development of its complication, nodular thyroid disease (NTD), remain unclear. This study aimed to investigate the role of miR-205-5p (miR-205-5p) in the development of nodular thyroid disease associated with coal worker's pneumoconiosis. The potential role of pulmonary extracellul
Google Scholar | PubMed | PMC | DOI
Deferoxamine prevents dexamethasone-induced muscle atrophy by reducing MuRF1 and atrogin-1.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Muscle atrophy, commonly triggered by glucocorticoids such as dexamethasone (DEX), involves increased protein degradation via the ubiquitin-proteasome system. Recent findings suggest that iron imbalance can also induce muscle atrophy. However, there have been no reports indicating that DEX causes intracellular iron imbalance leading to muscle atrophy. This study evaluated wheth
Google Scholar | PubMed | PMC | DOI
Comparative analysis of periodontal ligament stem cell characteristics in type 2 diabetic and non-diabetic patients with periodontitis.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Periodontitis, whether associated with diabetes or not, may have an impact on periodontal ligament stem cells (PDLSCs), which can adversely affect the regeneration of lost periodontal tissues and therefore treatment outcome. This study aimed to analyse the characteristics and differentiation potential of PDLSCs isolated from periodontitis patients with and without diabetes. To
Google Scholar | PubMed | PMC | DOI
Protease Enzyme Supplementation in Weaning Piglets Fed Reduced Crude Protein Diets: Effects on Gut Health Integrity and Performance Response.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Two trials evaluated the effects of dietary protease inclusion in weaned piglets fed diets with or without crude protein (CP) reduction, focusing on performance, intestinal health, and amino acid digestibility. In Trial I, 270 piglets (21-63 days) received six treatments: control (PC), PC with 100 g/ton protease A (PC+A), CP reduced by 1.0% (NC1) or 1.5% (NC1.5), NC1.5 with 50
Google Scholar | PubMed | PMC | DOI
Secure biosystems design in
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The widespread application of recombinant DNA and synthetic biology approaches for microbial metabolic engineering pursuits has motivated the development of biocontainment strategies, targeting safe and secure deployment of genetically modified microorganisms (GMMs). However, the design rules and mechanistic drivers governing biocontainment efficacy, as well as impacts of bioco
Google Scholar | PubMed | PMC | DOI
ATE1 promotes breast cancer progression via arginylation-dependent regulation of MAPK-MYC signaling.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Arginyl-tRNA-protein transferase (ATE1) catalyzes N-terminal arginylation, a regulatory protein modification implicated in various cellular processes, including proliferation, apoptosis, and migration. Although ATE1 has context-dependent roles in cancer, its specific function in breast cancer remains unclear. This study investigates the oncogenic role of ATE1 across multiple br
Google Scholar | PubMed | PMC | DOI
Habbe Gule Aakh prevents glycolytic program and alleviates disease progression in a rheumatoid arthritis animal model.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The acquired tumorigenic phenotype of the resident fibroblast like synoviocytes (FLS) is cornerstone to exacerbating rheumatoid arthritis (RA) disease progression. Toll like receptor 4 (TLR4) signalling can sustain the proliferative and invasive phenotype of these synoviocytes resulting in cartilage degradation and bone damage. A marked increase in glycolytic activity also cont
Google Scholar | PubMed | PMC | DOI
Rapalink-1 reveals TOR-dependent genes and an agmatinergic axis-based metabolic feedback regulating TOR activity and lifespan in fission yeast.
Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The Target of Rapamycin, TOR, is a conserved signalling pathway with characterised chemical inhibitors such as rapamycin and torin1. Bi-steric third-generation inhibitors, such as rapalink-1 have been developed, however, their effects on organismal gene expression and lifespan have not been characterised. Here, we demonstrate that rapalink-1 affects fission yeast spatial and te
Google Scholar | PubMed | PMC | DOI
Role of Branched-Chain Amino Acids in Mitigating Osteosarcopenia: An Experimental Study Using Ovariectomised Mice Models.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Osteosarcopenia, characterised by concurrent bone loss and muscle atrophy, presents a significant challenge in aging populations, particularly in postmenopausal women. The current therapeutic options potentially treat bone and muscle loss independently, highlighting the importance of an integrated approach. This study aimed to investigate the effects of branched-chain amino aci
Google Scholar | PubMed | PMC | DOI
Benchmarking porcine pancreatic ductal organoids for drug screening applications.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Primary human pancreatic ductal organoids (HPDO) have emerged as a model to study pancreas biology and model disease like pancreatitis and pancreatic cancer. Yet, donor material availability, genetic variability and a lack of extensive benchmarking to healthy and disease pancreas limits the range of applications. To address this gap, we established porcine pancreatic ductal org
Google Scholar | PubMed | PMC | DOI
Exposure to the trichloroethylene metabolite S-(1,2-dichlorovinyl)-L-cysteine under hypoxic conditions (%O
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Trichloroethylene (TCE) is a volatile organic compound used as an industrial solvent until the recent EPA phaseout. Despite regulatory restrictions, legacy TCE pollution persists in soil and groundwater and poses a risk to human health. This chemical exerts its toxic effects through its metabolites including the glutathione conjugation metabolite S-(1,2-dichlorovinyl)-L-cystein
Google Scholar | PubMed | PMC | DOI
Corneal Nerve Regeneration via MSC-Derived EVs: Tissue Source and Culture Dimensionality Dictate miRNA Cargo and Therapeutic Efficacy.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Extracellular vesicles (EVs) are emerging as critical mediators of intercellular communication and tissue repair, offering a promising cell-free platform for regenerative therapies. In the cornea, sensory nerves are critical for maintaining epithelial integrity and ocular homeostasis. Nerve injury resulting from trauma, surgery, or disease leads to persistent epithelial defects
Google Scholar | PubMed | PMC | DOI
Bioinspired pH-sensitive liposomes for quercetin delivery to synergize with 5- FU in gastric cancer therapy.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Gastric cancer is a major cause of cancer-related mortality on a global scale. Although 5-fluorouracil (5-FU) is a cornerstone chemotherapeutic for digestive tract malignancies, its efficacy is limited by dose-dependent toxicity and acquired resistance. Quercetin (QUC), a natural flavonoid, can sensitize tumor cells to 5-FU by modulating cell cycle-regulatory proteins. However,
Google Scholar | PubMed | PMC | DOI
Impact of Alpha-mangostin on human esophageal adenocarcinoma cells reveals activation of key apoptotic signaling pathways.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Current chemotherapeutic modalities for the clinical management of esophageal adenocarcinoma, do not, in most cases, result in complete remission and also elicit adverse side effects. In this context, active entities from natural products that have anticancer activity need to be developed. These drugs could also have the benefit of fewer side effects. We opted to study the effe
Google Scholar | PubMed | PMC | DOI
PCSK9 inhibition ameliorates microplastic-induced endothelial redox imbalance via SIRT6 modulation.
Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Microplastics (MPs) have emerged as significant environmental pollutants, posing a threat to ecosystems and humans. The presence of MPs in atherosclerotic plaques, exacerbating cardiovascular risk, has been recently reported. However, the molecular mechanism underlying the effects of MPs on the vascular endothelium are still undefined. In this regard, this study aims to investi
Google Scholar | PubMed | PMC | DOI
Karyotypic Profiling of Induced Pluripotent Stem Cells Derived from a Xeroderma Pigmentosum Group C Patient.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Xeroderma Pigmentosum group C (XP-C) is an autosomal recessive disorder caused by mutations in the
Google Scholar | PubMed | PMC | DOI
Tuning Poly(dimethylsiloxane) Hydrophilization and Coating Stability via the Optimization of Polyethylene Glycol Molecular Weight.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Polydimethylsiloxane (PDMS) is widely used in microfluidics and medical devices; however, its inherent hydrophobicity limits its applications. This can be resolved by the formation of polyethylene glycol (PEG)-based hydrophilic coatings. Here, we aimed to prove that PDMS surfaces modified with low molecular weight PEG (400) provided a more stable hydrophilic surface. The lowest
Google Scholar | PubMed | PMC | DOI
Single-cell RNA-sequencing-guided reactive oxygen species-scavenging hydrogel design for regeneration of osteoporotic bone.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
The pathological microenvironment of osteoporosis poses a substantial clinical challenge for bone defect regeneration. Through single-cell RNA-sequencing (scRNA-seq) analysis, we identified a reactive oxygen species (ROS)-overloading osteoblast subpopulation as a critical pathological feature of osteoporotic niches. Guided by scRNA-seq analysis, we engineered a microenvironment
Google Scholar | PubMed | PMC | DOI
Shiga Toxin Induces Apoptosis
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Hemolytic uremic syndrome (HUS), a fatal complication of Shiga toxin-producing
Google Scholar | PubMed | PMC | DOI
METTL16 Inhibits Lens Epithelial Cells Function in Diabetic Cataract via m6A-Modified DKK1-Mediated Wnt/β-Catenin Signaling.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
To explore the role of methyltransferase-like protein 16 (METTL16), an N6-methyladenosine (m6A) methyltransferase, in the development and progression of diabetic cataracts (DCs), as its underlying molecular mechanisms remain unknown. We evaluated m6A methylation levels in total RNA isolated from DC anterior capsule tissue and high glucose (HG)-induced human lens epithelial cell
Google Scholar | PubMed | PMC | DOI
Nanoplastic toxicity and uptake in kidney cells: differential effects of concentration, particle size, and polymer type.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Nanoplastics (NPs, < 1 µm) are emerging environmental contaminants capable of crossing biological barriers and interacting at the cellular and subcellular level. Despite evidence of microplastics in human kidney tissue and urine, the renal effects of NPs remain poorly understood. This study investigated the short-term effects of NPs polymer type, size, and concentration on huma
Google Scholar | PubMed | PMC | DOI
CSF1R marks a subset of foetal haematopoietic multipotent progenitor cells with acute myeloid leukaemia propagation properties.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
KMT2A-rearranged infant leukaemia is one of the most severe malignancies in infants and children, and is characterised by a very aggressive phenotype and lineage plasticity. KMT2A::MLLT3 is among the most common translocations initiating leukaemia in infants, where it can manifest with a myeloid or lymphoid leukaemia phenotype. The cell-of-origin and the mechanisms driving line
Google Scholar | PubMed | PMC | DOI
Inhibition of FOS-Like Antigen 1 Reduces Chemoresistance to Temozolomide Through Stemness Reprogramming via IL-6/STAT3
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Glioblastoma (GBM) is the most lethal brain tumor, characterized by strong resistance to conventional therapies. Despite recent therapeutic advancements, overcoming chemoresistance remains a major challenge. Here, we identified FOS-like antigen 1 (FOSL1) as a novel therapeutic target in GBM, particularly in patients with resistance to conventional drugs, including temozolomide
Google Scholar | PubMed | PMC | DOI
STARTER: a stand-alone reconfigurable and translational organ-on-chip platform based on modularity and open design principles.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Organ-on-chips (OoC) have the potential to revolutionize drug testing. However, the fragmented landscape of existing OoC systems leads to wasted resources and collaboration barriers, slowing broader adoption. To unite the ecosystem, there is an urgent need for generic OoC platforms based on interoperability and modularity. Technology platforms based on open designs would enable
Google Scholar | PubMed | PMC | DOI
Single-cell exon deletion profiling reveals splicing events that shape gene expression and cell state dynamics.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Alternative splicing is a pervasive gene regulatory mechanism critical for diversifying the human proteome. To systematically investigate its role in cell fate determination, we develop scCHyMErA-Seq, a scalable CRISPR-based exon deletion screening platform integrated with 10x Genomics single-cell transcriptomic readouts. This tool enables efficient exon deletion while simultan
Google Scholar | PubMed | PMC | DOI
A VPS33B CRISPR knockout study: In vitro evidence of an adhesion defect.
Cell type: Epithelial | Technique: Brightfield + fluorescence | Disease/area: Cell Biology & Signalling
VPS33B is a ubiquitously expressed regulator of vesicular membrane fusion and protein sorting involved in a broad range of cellular functions from organelle biogenesis to the establishment of apicobasal polarity. Loss-of-function mutations in VPS33B cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, a rare autosomal recessive disorder with multi-organ involvemen
Google Scholar | PubMed | PMC | DOI
Dehydrocorydaline treatment suppresses Th17 differentiation via the mTOR-mediated STAT3-RORγt signaling in systemic lupus erythematosus.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
BACKGROUND: Recent studies have demonstrated the pivotal role of pathogenic Th17 cells during the target organ damage in systemic lupus erythematosus (SLE). Therefore, identifying Th17-related precision therapeutic targets is essential for developing effective treatments. METHODS: Potential targets of a novel alkaloid compound, Dehydrocorydaline (DHC), in SLE were investigated
Google Scholar | PubMed | PMC | DOI
Primary Cilia Are Required for Efficient BMP Signaling in Traumatic Heterotopic Ossification.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
<b>Background/Objectives:</b> Heterotopic ossification (HO), the aberrant formation of bone within soft tissues, arises either from rare genetic mutations or more commonly from traumatic insults. It is a major cause of morbidity not only in individuals harboring causative mutations, but also in those undergoing musculoskeletal surgery or trauma and in soldiers sustaining blast
Google Scholar | PubMed | PMC | DOI
A phosphorous-based dendrimer targets mitochondria and normalizes the keratinocyte proliferation/differentiation balance to improve psoriasis.
Cell type: Keratinocyte | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Psoriasis vulgaris is a common chronic inflammatory skin disease associated with hyperproliferation and defective differentiation of keratinocytes. Despite new effective treatments using biologicals targeting key cytokines or their receptors, innovative therapeutic approaches remain to be discovered. Here, psoriasis-like imiquimod-induced skin lesions in mice were improved when
Google Scholar | PubMed | PMC | DOI
Metabolite mimicry identifies butyrate analogs with select protective functions in the intestinal mucosa.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Microbial-derived short-chain fatty acids regulate a variety of pathways in the healthy colonic mucosa. In particular, butyrate serves as the primary energy source for colonocytes and regulates gene transcription by stabilizing the transcription factor hypoxia-inducible-factors (HIF) and functioning as a histone deacetylase (HDAC) inhibitor. A limitation of butyrate as a therap
Google Scholar | PubMed | PMC | DOI
Dasatinib and quercetin senolytic treatment delays early onset intervertebral disc degeneration in SM/J mice.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Genetic background is a major determinant of disc degeneration, a leading cause of chronic back pain and disability. Herein, we demonstrate that premature disc cell senescence contributes to early-onset degeneration in SM/J mice and test two systemic senotherapeutic strategies to mitigate it: Navitoclax (Nav.) and a cocktail of Dasatinib and Quercetin (DQ). While Nav. treatment
Google Scholar | PubMed | PMC | DOI
Keratinocytes regulate intraepithelial lymphocytes homing and mediate mucosal barrier integrity via JAK2/STAT3 signaling in oral lichen planus.
Cell type: Keratinocyte | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Oral lichen planus (OLP) is a common T-cell-mediated inflammatory disease affecting the oral mucosa. Intraepithelial lymphocytes (IELs), a unique subset of T cells, play a crucial role in regulating mucosal immune responses. However, the mechanisms by which keratinocytes (KCs) regulate the homing migration of OLP IELs and their involvement in mucosal barrier disruption remain u
Google Scholar | PubMed | PMC | DOI
In vitro and in vivo anti-piroplasm activity of PBT2 against Theileria annulata and Babesia microti.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Theileriosis and babesiosis remain major constraints to livestock production, and increasing drug resistance necessitates alternative therapeutic strategies. Here, we evaluated the metal ionophore PBT2 for anti-piroplasm activity. PBT2 inhibited proliferation of two Theileria annulata schizont infected cell lines with IC
Google Scholar | PubMed | PMC | DOI
Cell alignment on novel polymeric micro-hollow fiber membranes for neural and musculoskeletal tissue engineering.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Precise positioning and alignment of specific cell types, such as those in the central nervous system and the muscular system, is essential for their functional integration, their migration, and proliferation in vivo. Cell alignment in physiologically relevant tissue models and constructs is challenging to reproduce in vitro unless a three-dimensional scaffold is used. This stu
Google Scholar | PubMed | PMC | DOI
Prostatic fibroblast reprogramming by Interleukin-30 activates prostate cancer metastasis programs.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Cell Biology & Signalling
Prostate cancer (PC) progression and mortality are largely driven by therapeutic resistance, increasingly linked to dynamic tumor-stroma interactions. However, the molecular basis of cancer-fibroblast crosstalk remains incompletely understood, particularly the contribution of Interleukin (IL)-30, an emerging regulator of PC aggressiveness. Here, we demonstrate that IL30 produce
Google Scholar | PubMed | PMC | DOI