EVOS M5000 in Cell Biology Research — Wave 3 Part 3

Effect of

Cell type: general | Technique: general | Disease/area: general | Animals : an open access journal from MDPI (2023)

Palm oil (PO) is currently the most widely used fat source for food production, but insect fat from

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Senataxin helicase, the causal gene defect in ALS4, is a significant modifier of C9orf72 ALS G4C2 and arginine-containing dipeptide repeat toxicity.

Cell type: HEK293, neuron | Technique: general | Disease/area: general | Acta neuropathologica communications (2023)

Identifying genetic modifiers of familial amyotrophic lateral sclerosis (ALS) may reveal targets for therapeutic modulation with potential application to sporadic ALS. GGGGCC (G4C2) repeat expansions in the C9orf72 gene underlie the most common form of familia

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Crosstalk between Aldosterone and Glycation through Rac-1 Induces Diabetic Nephropathy.

Cell type: general | Technique: general | Disease/area: general | ACS omega (2023)

(Abstract unavailable)

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Factors Influencing Marker Expressions of Cultured Human Cord Blood-Derived Mast Cells.

Cell type: T cell | Technique: EdU proliferation | Disease/area: general | International journal of molecular sciences (2023)

Mast cells (MCs) are tissue-resident immune cells of a hematopoietic origin that play vital roles in innate and adaptive immunity. Human MCs can be isolated and differentiated from various tissue sources, including cord blood, when supplemented with cytokines

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Investigating proliferation and differentiation capacities of Hanwoo steer myosatellite cells at different passages for developing cell-cultured meat.

Cell type: general | Technique: general | Disease/area: general | Scientific reports (2023)

The aim of study was to investigate proliferation and differentiation capacities of Hanwoo myosatellite cells for the development of Hanwoo cell cultures. From P1 to P19, the number of live cells decreased and the cell size increased. It was confirmed that the

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(Title unavailable)

Cell type: microglia | Technique: general | Disease/area: general | International journal of molecular sciences (2023)

Extracellular vesicles (EVs) of protozoan parasites have diverse biological functions that are essential for parasite survival and host-parasite interactions. In this study, we characterized the functional properties of EVs from

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Vitamin B

Cell type: general | Technique: general | Disease/area: general | Science advances (2023)

Vitamin B

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Preclinical Study on Biodistribution of Mesenchymal Stem Cells after Local Transplantation into the Brain.

Cell type: MSC | Technique: general | Disease/area: general | International journal of stem cells (2023)

Therapeutic efficacy of mesenchymal stem cells (MSCs) is determined by biodistribution and engraftment

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Cyclic strain of poly (methyl methacrylate) surfaces triggered the pathogenicity of Candida albicans.

Cell type: general | Technique: general | Disease/area: general | Acta biomaterialia (2023)

Candida albicans is an opportunistic yeast and the primary etiological factor in oral candidiasis and denture stomatitis. The pathogenesis of C. albicans could be triggered by several variables, including environmental, nutritional, and biomaterial surface cue

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Fluorinated Silane-Modified Filtroporation Devices Enable Gene Knockout in Human Hematopoietic Stem and Progenitor Cells.

Cell type: T cell | Technique: CRISPR, knockout | Disease/area: general | ACS applied materials & interfaces (2023)

Intracellular delivery technologies that are cost-effective, non-cytotoxic, efficient, and cargo-agnostic are needed to enable the manufacturing of cell-based therapies as well as gene manipulation for research applications. Current technologies capable of del

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Rapid Characterization of the Functional and Pharmacological Consequences of Cantú Syndrome K

Cell type: HEK293, T cell | Technique: general | Disease/area: general | The Journal of pharmacology and experimental therapeutics (2023)

Gain-of-function of K

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Identification of a small-molecule inhibitor that selectively blocks DNA-binding by Trypanosoma brucei replication protein A1.

Cell type: general | Technique: general | Disease/area: general | Nature communications (2023)

Replication Protein A (RPA) is a broadly conserved complex comprised of the RPA1, 2 and 3 subunits. RPA protects the exposed single-stranded DNA (ssDNA) during DNA replication and repair. Using structural modeling, we discover an inhibitor, JC-229, that target

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A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs.

Cell type: endothelial | Technique: general | Disease/area: general | Cell reports (2023)

Inner ear disorders are among the most common congenital abnormalities; however, current tissue culture models lack the cell type diversity to study these disorders and normal otic development. Here, we demonstrate the robustness of human pluripotent stem cell

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Patients with sporadic FTLD exhibit similar increases in lysosomal proteins and storage material as patients with FTD due to GRN mutations.

Cell type: general | Technique: general | Disease/area: general | Acta neuropathologica communications (2023)

Loss of function progranulin (GRN) mutations are a major autosomal dominant cause of frontotemporal dementia (FTD). Patients with FTD due to GRN mutations (FTD-GRN) develop frontotemporal lobar degeneration with TDP-43 pathology type A (FTLD-TDP type A) and ex

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Chamigrane-Type Sesquiterpenes from

Cell type: general | Technique: general | Disease/area: general | Marine drugs (2023)

(Abstract unavailable)

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Genes expression in

Cell type: general | Technique: general | Disease/area: general | Fish and shellfish immunology reports (2023)

(Abstract unavailable)

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Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy.

Cell type: iPSC, neuron | Technique: CRISPR, knockout | Disease/area: general | Brain : a journal of neurology (2023)

Spinal bulbar muscular atrophy (SBMA), the first identified CAG-repeat expansion disorder, is an X-linked neuromuscular disorder involving CAG-repeat-expansion mutations in the androgen receptor (AR) gene. We utilized CRISPR-Cas9 gene editing to engineer novel

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Protective effects of Gypenoside XVII against cerebral ischemia/reperfusion injury via SIRT1-FOXO3A- and Hif1a-BNIP3-mediated mitochondrial autophagy.

Cell type: general | Technique: general | Disease/area: general | Journal of translational medicine (2022)

Mitochondrial autophagy maintains mitochondrial function and cellular homeostasis and plays a critical role in the pathological process of cerebral ischemia/reperfusion injury (CIRI). Whether Gypenoside XVII (GP17) has regulatory effects on mitochondrial autop

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BK

Cell type: general | Technique: general | Disease/area: general | Pharmaceutics (2022)

Duchenne muscular dystrophy (DMD) is a progressive hereditary disease caused by the absence of the dystrophin protein. This is secondarily accompanied by a dysregulation of ion homeostasis, in which mitochondria play an important role. In the present work, we

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Ethyl acetate produced by

Cell type: general | Technique: general | Disease/area: general | Frontiers in microbiology (2022)

In this study, an oomycete strain FQ01 of

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Severe congenital myasthenic syndromes caused by agrin mutations affecting secretion by motoneurons.

Cell type: neuron | Technique: EdU proliferation | Disease/area: general | Acta neuropathologica (2022)

Congenital myasthenic syndromes (CMS) are predominantly characterized by muscle weakness and fatigability and can be caused by a variety of mutations in genes required for neuromuscular junction formation and maintenance. Among them, AGRN encodes agrin, an ess

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Expanding the phenotypic variability of MORC2 gene mutations: From Charcot-Marie-Tooth disease to late-onset pure motor neuropathy.

Cell type: cell line, neuron | Technique: general | Disease/area: general | Human mutation (2022)

MORC2 gene encodes a ubiquitously expressed nuclear protein involved in chromatin remodeling, DNA repair, and transcriptional regulation. Heterozygous mutations in MORC2 gene have been associated with a spectrum of disorders affecting the peripheral nervous sy

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Fucosterol Isolated from Dietary Brown Alga

Cell type: general | Technique: general | Disease/area: general | Antioxidants (Basel, Switzerland) (2022)

(Abstract unavailable)

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Differences in the Autophagy Response to Hypoxia in the Hippocampus and Neocortex of Rats.

Cell type: neuron | Technique: immunohistochemistry | Disease/area: general | International journal of molecular sciences (2022)

Autophagy is a regulated mechanism of degradation of misfolded proteins and organelles in the cell. Neurons are highly differentiated cells with extended projections, and therefore, their functioning largely depends on the mechanisms of autophagy. For the firs

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Real-time monitoring of serotonin with highly selective aptamer-functionalized conducting polymer nanohybrids.

Cell type: general | Technique: general | Disease/area: general | Nano convergence (2022)

Adequate serotonin levels are pivotal to human well-being; thus, serotonin can be used as a biomarker because it regulates a wide range of physical and psychological functions. As an imbalance of serotonin is highly likely to initiate the pathogenesis of vario

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Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental Samples.

Cell type: general | Technique: general | Disease/area: general | Sensors (Basel, Switzerland) (2022)

The development of predictive

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Mapping cis-regulatory elements in human neurons links psychiatric disease heritability and activity-regulated transcriptional programs.

Cell type: neuron | Technique: general | Disease/area: general | Cell reports (2022)

Genome-wide association studies (GWASs) have identified hundreds of loci associated with psychiatric diseases, yet there is a lack of understanding of disease pathophysiology. Common risk variants can shed light on the underlying molecular mechanisms; however,

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IL-33 enhances Jagged1 mediated NOTCH1 intracellular domain (NICD) deubiquitination and pathological angiogenesis in proliferative retinopathy.

Cell type: endothelial | Technique: EdU proliferation | Disease/area: general | Communications biology (2022)

Pathological retinal neovascularization (NV) is a clinical manifestation of various proliferative retinopathies, and treatment of NV using anti-VEGF therapies is not selective, as it also impairs normal retinal vascular growth and function. Here, we show that

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Muscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue.

Cell type: general | Technique: general | Disease/area: general | NPJ science of food (2022)

Cultured meat is an emergent technology with the potential for significant environmental and animal welfare benefits. Accurate mimicry of traditional meat requires fat tissue; a key contributor to both the flavour and texture of meat. Here, we show that fibro-

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Human amniotic epithelial cell transplantation improves scar remodeling in a rabbit model of acute vocal fold injury: a pilot study.

Cell type: epithelial | Technique: general | Disease/area: general | Stem cell research & therapy (2022)

To gain insight into the molecular mechanisms underlying the early stages of vocal fold extracellular matrix (ECM) remodeling after a mid-membranous injury resulting from the use of human amniotic epithelial cells (hAEC), as a novel regenerative medicine cell-

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Blockade of GRTH/DDX25 Phosphorylation by Cyclic Peptides Provides an Avenue for Developing a Nonhormonal Male Contraceptive.

Cell type: general | Technique: general | Disease/area: general | Journal of medicinal chemistry (2021)

Gonadotropin-regulated testicular RNA helicase (GRTH)/DDX25 is a DEAD-box RNA helicase essential for the completion of spermatogenesis. Our previous studies indicated that blocking the GRTH phospho-site or perturbing the GRTH/protein kinase A (PKA) interface c

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Antifungal Effect of Piezoelectric Charges on PMMA Dentures.

Cell type: general | Technique: general | Disease/area: general | ACS biomaterials science & engineering (2021)

Candida-associated denture stomatitis is a recurring disease affecting up to 67% of denture wearers. Poly(methyl methacrylate) (PMMA) remains the main material employed in the fabrication of dentures due to its desirable physical, mechanical, and aesthetic pro

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A flow-cytometry-based assessment of global protein synthesis in human senescent cells.

Cell type: HEK293, T cell, cell line, fibroblast | Technique: EdU proliferation, flow cytometry | Disease/area: general | STAR protocols (2021)

Senescent cells constantly experience stressful conditions and restrain their protein translation to cope with it. Here, we present a detailed protocol to measure the rate of global protein synthesis using L-azidohomoalanine (L-AHA)-based click chemistry in hu

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Formoterol PLGA-PEG Nanoparticles Induce Mitochondrial Biogenesis in Renal Proximal Tubules.

Cell type: general | Technique: general | Disease/area: general | The AAPS journal (2021)

Formoterol is a long-acting β

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Cytotoxic and apoptotic data of BPA and BPA alternatives TMBPF, BPAF, and BPS in female adult rat and human stem cells.

Cell type: general | Technique: general | Disease/area: general | Data in brief (2021)

Here, we used female adult rat adipose-derived stem cells (rASCs) and human adipose-derived stem cells (hASCs) to compare the toxicities and potencies of several widespread environmental toxins that may be endocrine-disrupting chemicals, including bisphenol A

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Effect of F16-Betulin Conjugate on Mitochondrial Membranes and Its Role in Cell Death Initiation.

Cell type: general | Technique: general | Disease/area: general | Membranes (2021)

This work demonstrates the effects of a newly synthesized conjugate of the plant triterpenoid betulin and the penetrating cation F16 used for mitochondrial targeting. The resulting F16-betulin conjugate revealed a mitochondria-targeted effect, decreasing the m

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Lung on a Chip Development from Off-Stoichiometry Thiol-Ene Polymer.

Cell type: A549, cell line, endothelial | Technique: immunofluorescence | Disease/area: general | Micromachines (2021)

Current in vitro models have significant limitations for new respiratory disease research and rapid drug repurposing. Lung on a chip (LOAC) technology offers a potential solution to these problems. However, these devices typically are fabricated from polydimet

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Protocol to profile the bioenergetics of organoids using Seahorse.

Cell type: general | Technique: general | Disease/area: general | STAR protocols (2021)

Addressing bioenergetics is key to evaluate the impact of metabolism on the regulation of biological processes and its alteration in disease. Organoids are

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In Vitro Induction of Pluripotency from Equine Fibroblasts in 20% or 5% Oxygen.

Cell type: fibroblast | Technique: general | Disease/area: general | Stem cells international (2020)

The cellular reprogramming into pluripotency is influenced by external and internal cellular factors, such as

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