EVOS M5000 in Cancer Research: Cell Models, Imaging Techniques, and Disease Targets
Curated literature review of peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
From lung and breast tumours to uveal melanoma spheroids, the EVOS M5000 Imaging System has become a routine workhorse for oncology labs that need brightfield and fluorescence imaging without a darkroom. The papers below show how researchers use the system to visualise morphology, viability, migration, and treatment response across a range of cancer cell models.
TOPORS, a tumor suppressor protein, contributes to the maintenance of higher-order chromatin architecture.
Cell type: Cancer cells (U2OS/HeLa nuclear architecture)
Imaging technique: Fluorescence microscopy
Disease area: Cancer
In the nucleus, chromosomes are hierarchically folded into active (A) and inactive (B) compartments composed of topologically associating domains (TADs). Genomic regions interact with nuclear lamina, termed lamina-associated domains (LADs). However, the molecular mechanisms under...
Biochim Biophys Acta Gene Regul Mech β PMID 32113985
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The Killing of Human Neuroblastoma Cells by the Small Molecule JQ1 Occurs in a p53-Dependent Manner.
Cell type: Neuroblastoma cells (neuronal cancer)
Imaging technique: Viability / fluorescence imaging
Disease area: Neuroblastoma
BACKGROUND: MYCN amplification is a prognostic biomarker associated with poor prognosis of neuroblastoma in children. The overall survival of children with MYCN-amplified neuroblastoma has only marginally improved within the last 20 years. The Bromodomain and Extra-Terminal motif...
Anticancer Agents Med Chem β PMID 32329693
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STAT3-induced long noncoding RNA LINC00668 promotes migration and invasion of non-small cell lung cancer via the miR-193a/KLF7 axis.
Cell type: Non-small cell lung cancer cells
Imaging technique: Migration/invasion assays, live-cell imaging
Disease area: Non-small cell lung cancer
Long noncoding RNAs (lncRNAs) have been demonstrated to play significant roles in non-small cell lung cancer (NSCLC) progression. Recently, a newly identified lncRNA, LncRNA LINC00668 (LINC00668), was reported to be involved in the regulation of progression of several tumors. How...
Biomed Pharmacother β PMID 31150989
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Decreased expression of miR-551b predicts poor prognosis and promotes tumorigenesis by targeting PTP4A3 in human colorectal cancer.
Cell type: Colorectal cancer cells
Imaging technique: Proliferation/viability, fluorescence imaging
Disease area: Colorectal cancer
OBJECTIVE: MiR-551b has been reported to display tumor-suppressive and oncogenic potential in several cancers, but there has been no study on the roles of miR-551b in colorectal cancer (CRC). In this work, we aimed to explore the potential functions and mechanisms of miR-551b in ...
Eur Rev Med Pharmacol Sci β PMID 31298325
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Transcriptional repression of SIRT3 potentiates mitochondrial aconitase activation to drive aggressive prostate cancer to the bone.
Cell type: Prostate cancer cells
Imaging technique: Metastasis imaging / fluorescence
Disease area: Prostate cancer / bone metastasis
Metabolic dysregulation is a known hallmark of cancer progression, yet the oncogenic signals that promote metabolic adaptations to drive metastatic cancer remain unclear. Here, we show that transcriptional repression of mitochondrial deacetylase sirtuin 3 (SIRT3) by androgen rece...
Cancer Res β PMID 33115805
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Hydrazinocurcumin and 5-fluorouracil enhance apoptosis and restrain tumorigenicity of HepG2 cells via disrupting the PTEN-mediated PI3K/Akt signaling pathway.
Cell type: HepG2 hepatocellular carcinoma cells
Imaging technique: Apoptosis/viability, fluorescence imaging
Disease area: Liver cancer
Hydrazinocurcumin (HZC), a curcumin analogue, serves as a tumor suppressor in breast cancer and lung cancer. In this study, we investigate the role and mechanism of HZC in regulating HepG2 cell apoptosis and tumorigenicity, and its synergistic effects with 5-fluorouracil (5-Fu). ...
Biomed Pharmacother β PMID 32559627
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Golgi Acidification by NHE7 Regulates Cytosolic pH Homeostasis in Pancreatic Cancer Cells.
Cell type: Pancreatic cancer cells
Imaging technique: Live-cell fluorescence (pH sensors)
Disease area: Pancreatic cancer
Cancer cells reprogram their metabolism to meet elevated energy demands and favor glycolysis for energy production. This boost in glycolytic flux supports proliferation, but also generates acid in the form of hydrogen ions that must be eliminated from the cytoplasm to maintain th...
Cancer Discov β PMID 32200349
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TRIM11 promotes breast cancer cell proliferation by stabilizing estrogen receptor a.
Cell type: Breast cancer cells (ERΞ±+)
Imaging technique: Proliferation, fluorescence imaging
Disease area: Breast cancer
Breast cancer is the most commonly diagnosed malignancy in female worldwide, over 70% of which are estrogen receptor Ξ± (ERΞ±) positive. ERΞ± has a crucial role in the initiation and progression of breast cancer and is an indicator of endocrine therapy, while endocrine resistance is...
Neoplasia β PMID 32599554
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Characterization of Uveal Melanoma Cell Lines and Primary Tumor Samples in 3D Culture.
Cell type: Uveal melanoma cells
Imaging technique: 3D spheroid, live-cell/fluorescence
Disease area: Uveal melanoma / eye cancer
PURPOSE: Uveal melanoma (UM) typically spreads to the liver, where it is incurable, as there are limited therapeutic interventions available. This study aimed to standardize laboratory methods for generating three-dimensional (3D) spheroids using UM cell lines and primary UM (PUM...
Transl Vis Sci Technol β PMID 32832244
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The intensification of anticancer activity of LFM-A13 by erythropoietin as a possible option for inhibition of breast cancer.
Cell type: Breast cancer cells
Imaging technique: Viability/proliferation, fluorescence
Disease area: Breast cancer
Recombinant human erythropoietin (Epo) is an effective and convenient treatment for cancer-related anaemia. In our study for the first time, we evaluated the effect of simultaneous use of Epo and Bruton's tyrosine kinase (BTK) inhibitor LFM-A13 on the viability and tumour develop...
J Enzyme Inhib Med Chem β PMID 32912025
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