EVOS M5000 in Cardiovascular and Endothelial Research

Curated literature review of peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.

Cardiovascular research often sits at the intersection of live-cell imaging, 3D hydrogel models, and long-term phenotype tracking. The EVOS M5000's on-stage incubation and easy-to-share image formats make it a practical choice for endothelial, smooth-muscle, and cardiomyocyte work.

Sirtuin 6 deficiency induces endothelial cell senescence via downregulation of forkhead box M1 expression.

Cell type: Endothelial cells

Imaging technique: Fluorescence/senescence imaging

Disease area: Atherosclerosis / vascular aging

Cellular senescence of endothelial cells causes vascular dysfunction, promotes atherosclerosis, and contributes to the development of age-related vascular diseases. Sirtuin 6 (SIRT6), a conserved NAD+-dependent protein deacetylase, has beneficial effects against aging, despite th...

Aging (Albany NY) — PMID 33171439

Google Scholar | PubMed | DOI

Dysfunction of iPSC-derived endothelial cells in human Hutchinson-Gilford progeria syndrome.

Cell type: iPSC-derived endothelial cells

Imaging technique: Brightfield/fluorescence imaging

Disease area: Hutchinson-Gilford progeria / cardiovascular aging

Children with Hutchinson-Gilford progeria syndrome (HGPS) succumb to myocardial infarction and stroke in their teen years. Endothelial dysfunction is an early event in more common forms of atherosclerosis. Endothelial pathobiology may contribute to HGPS, but a comprehensive chara...

Cell Cycle — PMID 31411525

Google Scholar | PubMed | DOI

Three-dimensional encapsulation of adult mouse cardiomyocytes in hydrogels with tunable stiffness.

Cell type: Adult mouse cardiomyocytes

Imaging technique: 3D hydrogel encapsulation, live-cell imaging

Disease area: Cardiovascular / heart disease

Numerous diseases, including those of the heart, are characterized by increased stiffness due to excessive deposition of extracellular matrix proteins. Cardiomyocytes continuously adapt their morphology and function to the mechanical changes of their microenvironment. Because tra...

Prog Biophys Mol Biol — PMID 31122749

Google Scholar | PubMed | DOI

Endothelial cell-derived extracellular vesicles alter vascular smooth muscle cell phenotype through high-mobility group box proteins.

Cell type: Endothelial cells and vascular smooth muscle cells

Imaging technique: Exosome/vesicle imaging, fluorescence

Disease area: Cardiovascular disease

The vascular endothelium and smooth muscle form adjacent cellular layers that comprise part of the vascular wall. Each cell type can regulate the other's structure and function through a variety of paracrine effectors. Extracellular vesicles (EVs) are released from and transit be...

J Extracell Vesicles — PMID 32944170

Google Scholar | PubMed | DOI

STAT3-induced up-regulation of lncRNA NEAT1 as a ceRNA facilitates abdominal aortic aneurysm formation by elevating TULP3.

Cell type: Vascular smooth muscle cells / aortic tissue

Imaging technique: Live-cell / fluorescence imaging

Disease area: Abdominal aortic aneurysm

Long noncoding RNAs (lncRNAs) were viewed as crucial participants in the pathogenesis of abdominal aortic aneurysm (AAA). LncRNA NEAT1 was recognized as an oncogenic gene in various diseases. However, its function and mechanism in AAA were not precisely documented. Here, we explo...

Biosci Rep — PMID 31868202

Google Scholar | PubMed | DOI