EVOS M5000 in Cell Biology and Intracellular Signaling
Curated literature review of peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
Beyond disease-specific models, the EVOS M5000 is used to interrogate fundamental cell biology: engineered protein sensors, WNT/β-catenin reporters, and nuclear speckle organisation.
Engineering Intracellular Protein Sensors in Mammalian Cells.
Cell type: Mammalian cells (engineered protein sensors)
Imaging technique: Fluorescence microscopy
Disease area: Neurodegenerative / metabolic disease models
Proteins can function as biomarkers of pathological conditions, such as neurodegenerative diseases, infections or metabolic syndromes. Engineering cells to sense and respond to these biomarkers may help the understanding of molecular mechanisms underlying pathologies, as well as ...
J Vis Exp — PMID 32420982
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What does this study conclude about Neurodegenerative / metabolic disease models? Proteins can function as biomarkers of pathological conditions, such as neurodegenerative diseases, infections or metabolic syndromes. Engineering cells to sense and respond to these biomarkers may help the understanding of molecular mechanisms underlying pathologies, as well as .
What role did the EVOS M5000 play in the result? The EVOS M5000 imaged the fluorescent protein-sensor constructs inside mammalian cells, so the researchers could verify that the engineered sensors localised and reported on the target pathological biomarkers as designed.
R-spondins engage heparan sulfate proteoglycans to potentiate WNT signaling.
Cell type: Cultured cells (WNT signaling reporter)
Imaging technique: Fluorescence/bioluminescence reporter imaging
Disease area: Developmental / regenerative biology
R-spondins (RSPOs) amplify WNT signaling during development and regenerative responses. We previously demonstrated that RSPOs 2 and 3 potentiate WNT/β-catenin signaling in cells lacking leucine-rich repeat-containing G-protein coupled receptors (LGRs) 4, 5 and 6 (Lebensohn and Ro...
Elife — PMID 32432544
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Frequently asked questions about this study
What does this study conclude about Developmental / regenerative biology? R-spondins (RSPOs) amplify WNT signaling during development and regenerative responses. We previously demonstrated that RSPOs 2 and 3 potentiate WNT/β-catenin signaling in cells lacking leucine-rich repeat-containing G-protein coupled receptors (LGRs) 4, 5 and 6 (Lebensohn and Ro.
How did the EVOS M5000 help make this discovery? The EVOS M5000 detected the fluorescent and bioluminescent reporter outputs that showed how R-spondins amplify WNT/β-catenin signalling, making the signalling potentiation visible and quantifiable.
SON and SRRM2 are essential for nuclear speckle formation.
Cell type: Cultured human cells
Imaging technique: Fluorescence microscopy
Disease area: Cancer / basic cell biology
Nuclear speckles (NS) are among the most prominent biomolecular condensates. Despite their prevalence, research on the function of NS is virtually restricted to colocalization analyses, since an organizing core, without which NS cannot form, remains unidentified. The monoclonal a...
Elife — PMID 33095160
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What does this study conclude about Cancer / basic cell biology? Nuclear speckles (NS) are among the most prominent biomolecular condensates. Despite their prevalence, research on the function of NS is virtually restricted to colocalization analyses, since an organizing core, without which NS cannot form, remains unidentified.
Why was the EVOS M5000 important for reaching this conclusion? Fluorescence imaging on the EVOS M5000 revealed the loss or reorganisation of nuclear speckles when SON and SRRM2 were depleted, providing the direct structural evidence that these proteins form the speckle-organising core.