EVOS M5000 in Reproductive and Developmental Biology

Curated literature review of peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.

Reproductive and developmental biology requires gentle, long-term imaging of primary cells and tissues. The EVOS M5000 supports studies of trophoblasts, placental macrophages, and gametes with benchtop simplicity.

Long Non-Coding RNA Nuclear-Enriched Abundant Transcript 1 (NEAT1) Represses Proliferation of Trophoblast Cells in Rats with Preeclampsia via the MicroRNA-373/FLT1 Axis.

Cell type: Trophoblast cells

Imaging technique: Live-cell / fluorescence microscopy

Disease area: Preeclampsia

BACKGROUND Preeclampsia (PE) remains one of the primary causes of maternal morbidity and mortality worldwide. This study was designed to investigate the relevance of long non-coding RNA (lncRNA) nuclear-enriched abundant transcript 1 (NEAT1) and downstream molecules in trophoblas...

Med Sci Monit — PMID 33093438

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Effect of microfluidic processing on the viability of boar and bull spermatozoa.

Cell type: Boar and bull spermatozoa

Imaging technique: Live-cell / brightfield imaging

Disease area: Reproductive / infertility

The use of microfluidics in artificial reproductive technologies for manipulation or assessment of spermatozoa is unique in the sense that it is not always an end point measurement and the sample may be used afterward. During microfluidic processing, spermatozoa are exposed to sh...

Biomicrofluidics — PMID 32774586

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Phenotypic and functional characterization of first-trimester human placental macrophages, Hofbauer cells.

Cell type: Placental macrophages (Hofbauer cells)

Imaging technique: Phenotypic brightfield/fluorescence imaging

Disease area: Pregnancy / placental immunology

Hofbauer cells (HBCs) are a population of macrophages found in high abundance within the stroma of the first-trimester human placenta. HBCs are the only fetal immune cell population within the stroma of healthy placenta. However, the functional properties of these cells are poorl...

J Exp Med — PMID 33075123

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