EVOS M5000 in Reproductive and Developmental Biology
Curated literature review of peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
Reproductive and developmental biology requires gentle, long-term imaging of primary cells and tissues. The EVOS M5000 supports studies of trophoblasts, placental macrophages, and gametes with benchtop simplicity.
Long Non-Coding RNA Nuclear-Enriched Abundant Transcript 1 (NEAT1) Represses Proliferation of Trophoblast Cells in Rats with Preeclampsia via the MicroRNA-373/FLT1 Axis.
Cell type: Trophoblast cells
Imaging technique: Live-cell / fluorescence microscopy
Disease area: Preeclampsia
BACKGROUND Preeclampsia (PE) remains one of the primary causes of maternal morbidity and mortality worldwide. This study was designed to investigate the relevance of long non-coding RNA (lncRNA) nuclear-enriched abundant transcript 1 (NEAT1) and downstream molecules in trophoblas...
Med Sci Monit — PMID 33093438
Google Scholar | PubMed
Frequently asked questions about this study
How does lncRNA NEAT1 influence trophoblast cell proliferation in preeclampsia? Long non-coding RNA NEAT1 represses proliferation of trophoblast cells in preeclamptic rats by acting through the microRNA-373/FLT1 axis.
How did the EVOS M5000 capture the trophoblast proliferation phenotype? Live-cell and fluorescence microscopy on the EVOS M5000 recorded trophoblast cell number, nuclear morphology and marker expression, providing the visual evidence linking NEAT1 modulation to proliferation changes.
Effect of microfluidic processing on the viability of boar and bull spermatozoa.
Cell type: Boar and bull spermatozoa
Imaging technique: Live-cell / brightfield imaging
Disease area: Reproductive / infertility
The use of microfluidics in artificial reproductive technologies for manipulation or assessment of spermatozoa is unique in the sense that it is not always an end point measurement and the sample may be used afterward. During microfluidic processing, spermatozoa are exposed to sh...
Biomicrofluidics — PMID 32774586
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What effect does microfluidic processing have on boar and bull spermatozoa viability? Microfluidic processing exposes spermatozoa to shear and flow stresses that can affect viability, so the study assessed whether processed boar and bull sperm remain usable for downstream assisted-reproductive procedures.
How does the EVOS M5000 help judge sperm viability after microfluidic handling? Brightfield and simple fluorescence viability staining on the EVOS M5000 lets researchers quickly count live, motile and morphologically intact spermatozoa after microfluidic sorting or manipulation.
Phenotypic and functional characterization of first-trimester human placental macrophages, Hofbauer cells.
Cell type: Placental macrophages (Hofbauer cells)
Imaging technique: Phenotypic brightfield/fluorescence imaging
Disease area: Pregnancy / placental immunology
Hofbauer cells (HBCs) are a population of macrophages found in high abundance within the stroma of the first-trimester human placenta. HBCs are the only fetal immune cell population within the stroma of healthy placenta. However, the functional properties of these cells are poorl...
J Exp Med — PMID 33075123
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What are Hofbauer cells, and what did this study characterise about them? Hofbauer cells are placental macrophages abundant in first-trimester human placenta; this study characterised their phenotype and function as the only fetal immune cell population in healthy placental stroma.
How did the EVOS M5000 support phenotypic characterisation of Hofbauer cells? Brightfield and multi-channel fluorescence imaging on the EVOS M5000 captured Hofbauer cell morphology and macrophage marker expression during phenotypic and functional assays of isolated placental macrophages.