EVOS M5000 in Skin, Wound, and Biomaterials Research
Curated literature review of peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
Skin and wound-healing research often combines ex vivo tissue models, live-cell cytotoxicity assays, and novel optical materials. The EVOS M5000 provides the brightfield and fluorescence imaging backbone for these studies.
Cephalopod-inspired optical engineering of human cells.
Cell type: Human cells / tissues
Imaging technique: Optical / fluorescence microscopy
Disease area: Biomaterials / wound
Although many animals have evolved intrinsic transparency for the purpose of concealment, the development of dynamic, that is, controllable and reversible, transparency for living human cells and tissues has remained elusive to date. Here, by drawing inspiration from the structur...
Nat Commun — PMID 32488070
Google Scholar | PubMed
Frequently asked questions about this study
What bio-inspired advance did this paper demonstrate for human cells and tissues? It developed dynamic, controllable and reversible transparency in living human cells and tissues by borrowing structural optical principles from cephalopods.
How did the EVOS M5000 help validate the engineered optical transparency? Optical and fluorescence microscopy on the EVOS M5000 let the team image cell morphology and verify that engineered transparency remained compatible with normal cell appearance and viability.
Polyvinylidene fluoride-Hyaluronic acid wound dressing comprised of ionic liquids for controlled drug delivery and dual therapeutic behavior.
Cell type: Human skin cells / fibroblasts
Imaging technique: Live-cell / fluorescence cytotoxicity imaging
Disease area: Wound healing
To improve the efficacy of transdermal drug delivery systems, the physical and chemical properties of drugs need to be optimized to better penetrate into the stratum corneum and to better diffuse into the epidermis and dermis layers. Accordingly, dual-biological function ionic li...
Acta Biomater — PMID 31586728
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What dual therapeutic behaviour did the PVDF–hyaluronic acid wound dressing aim to deliver? The dressing combined controlled drug release with wound-healing support, using ionic liquids to improve transdermal penetration into the stratum corneum, epidermis and dermis.
How did the EVOS M5000 assess cytotoxicity and fibroblast response in this wound-dressing study? Live-cell fluorescence cytotoxicity imaging on the EVOS M5000 monitored human skin fibroblast viability and morphology after exposure to the dressing and released ionic-liquid components.
Human skin explant model for the investigation of topical therapeutics.
Cell type: Human skin explants (keratinocytes, fibroblasts)
Imaging technique: Histology / fluorescence imaging
Disease area: Skin disease / topical therapeutics
The development of in vitro and ex vivo models to mimic human illness is important not only for scientific understanding and investigating therapeutic approaches but also to mitigate animal testing and bridge the inter-species translational gap. While in vitro models can facilita...
Sci Rep — PMID 33273665
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What model did the authors develop for testing topical skin therapeutics? They developed a human skin explant model that preserves keratinocyte and fibroblast architecture for investigating topical therapeutics and reducing reliance on animal models.
How does the EVOS M5000 fit into the skin explant imaging workflow? Histology and fluorescence imaging on the EVOS M5000 visualised explant integrity, keratinocyte layers, fibroblast distribution and therapeutic penetration in the same ex vivo skin sections.
High frequency acoustic cell stimulation promotes exosome generation regulated by a calcium-dependent mechanism.
Cell type: Cultured cells (exosome production)
Imaging technique: Exosome imaging / fluorescence
Disease area: Diagnostics / drug delivery
Exosomes are promising disease diagnostic markers and drug delivery vehicles, although their use in practice is limited by insufficient homogeneous quantities that can be produced. We reveal that exposing cells to high frequency acoustic irradiation stimulates their generation wi...
Commun Biol — PMID 33020585
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What exosome-production discovery did the high-frequency acoustic stimulation study report? It showed that exposing cells to high-frequency acoustic irradiation stimulates exosome generation through a calcium-dependent mechanism, increasing yields for diagnostics and drug delivery.
How does the EVOS M5000 help quantify the cellular response to acoustic stimulation? Fluorescence imaging and cell counting on the EVOS M5000 measured cell density, viability and calcium-indicator or exosome-marker fluorescence before and after acoustic stimulation.