EVOS M5000 in Regenerative Medicine: Muscle, Bone, and Stem Cell Research
Curated literature review of peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
Regenerative medicine depends on watching cells differentiate, proliferate, and interact with biomaterials. The EVOS M5000 is frequently cited for brightfield and fluorescence monitoring of myoblasts, mesenchymal stem cells, osteoblasts, and 3D scaffold cultures.
The panniculus carnosus muscle: A novel model of striated muscle regeneration that exhibits sex differences in the mdx mouse.
Cell type: Striated muscle myofibers (mouse panniculus carnosus)
Imaging technique: Brightfield/phase-contrast morphology imaging
Disease area: Muscular dystrophy (mdx model)
The dermal striated muscle panniculus carnosus (PC), prevalent in lower mammals with remnants in humans, is highly regenerative, and whose function is purported to be linked to defence and shivering thermogenesis. Given the heterogeneity of responses of different muscles to disea...
Sci Rep — PMID 31685850
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3D Printed Polyurethane Scaffolds for the Repair of Bone Defects.
Cell type: Bone marrow mesenchymal stem cells / osteoblasts
Imaging technique: 3D culture, fluorescence/brightfield imaging
Disease area: Bone defect / orthopaedic
Critical-size bone defects are those that will not heal without intervention and can arise secondary to trauma, infection, and surgical resection of tumors. Treatment options are currently limited to filling the defect with autologous bone, of which there is not always an abundan...
Front Bioeng Biotechnol — PMID 33195122
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ZNF521 Represses Osteoblastic Differentiation in Human Adipose-Derived Stem Cells.
Cell type: Adipose-derived stem cells / osteoblasts
Imaging technique: Brightfield/fluorescence imaging
Disease area: Bone regeneration
Human adipose-derived stem cells (hADSCs) are multipotent mesenchymal cells that can differentiate into adipocytes, chondrocytes, and osteocytes. During osteoblastogenesis, the osteoprogenitor cells differentiate into mature osteoblasts and synthesize bone matrix components. Zinc...
Int J Mol Sci — PMID 30567301
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Screening method to identify hydrogel formulations that facilitate myotube formation from encapsulated primary myoblasts.
Cell type: Primary myoblasts
Imaging technique: 3D hydrogel culture, live-cell imaging
Disease area: Muscle regeneration / muscular dystrophy
Hydrogel-based three-dimensional (3D) cellular models are attractive for bioengineering and pharmaceutical development as they can more closely resemble the cellular function of native tissue outside of the body. In general, these models are composed of tissue specific cells embe...
Bioeng Transl Med — PMID 33005743
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Real-time tracking of stem cell viability, proliferation, and differentiation with autonomous bioluminescence imaging.
Cell type: Stem cells (mesenchymal)
Imaging technique: Bioluminescence imaging, live-cell
Disease area: Regenerative medicine / cell therapy
BACKGROUND: Luminescent reporter proteins are vital tools for visualizing cells and cellular activity. Among the current toolbox of bioluminescent systems, only bacterial luciferase has genetically defined luciferase and luciferin synthesis pathways that are functional at the mam...
BMC Biol — PMID 32620121
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Essential oil impregnated luminescent hydroxyapatite: Antibacterial and cytotoxicity studies.
Cell type: Bacteria / mammalian cells
Imaging technique: Fluorescence microscopy
Disease area: Infection / bone repair
In this study, porous fluorescent nanocrystalline erbium doped hydroxyapatite (eHAp) was synthesized via hydrothermal assisted co-precipitation method. Eucalyptus oil (EU), frankincense oil (FO), Tea tree oil (TTO), wintergreen oil (WO) were successfully absorbed into eHAp pellet...
Mater Sci Eng C Mater Biol Appl — PMID 32806309
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