EVOS M5000 in Regenerative Medicine: Muscle, Bone, and Stem Cell Research
Curated literature review of peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
Regenerative medicine depends on watching cells differentiate, proliferate, and interact with biomaterials. The EVOS M5000 is frequently cited for brightfield and fluorescence monitoring of myoblasts, mesenchymal stem cells, osteoblasts, and 3D scaffold cultures.
The panniculus carnosus muscle: A novel model of striated muscle regeneration that exhibits sex differences in the mdx mouse.
Cell type: Striated muscle myofibers (mouse panniculus carnosus)
Imaging technique: Brightfield/phase-contrast morphology imaging
Disease area: Muscular dystrophy (mdx model)
The dermal striated muscle panniculus carnosus (PC), prevalent in lower mammals with remnants in humans, is highly regenerative, and whose function is purported to be linked to defence and shivering thermogenesis. Given the heterogeneity of responses of different muscles to disea...
Sci Rep — PMID 31685850
Google Scholar | PubMed
Frequently asked questions about this study
What makes the panniculus carnosus a useful model for striated muscle regeneration? The dermal striated muscle panniculus carnosus is highly regenerative in lower mammals, retains remnants in humans, and shows sex-different responses in the mdx muscular dystrophy mouse.
How does the EVOS M5000 capture regeneration in this muscle model? Brightfield and phase-contrast morphology imaging on the EVOS M5000 lets researchers monitor myofiber morphology and regenerative changes in cultured or sectioned panniculus carnosus tissue without complex confocal setup.
3D Printed Polyurethane Scaffolds for the Repair of Bone Defects.
Cell type: Bone marrow mesenchymal stem cells / osteoblasts
Imaging technique: 3D culture, fluorescence/brightfield imaging
Disease area: Bone defect / orthopaedic
Critical-size bone defects are those that will not heal without intervention and can arise secondary to trauma, infection, and surgical resection of tumors. Treatment options are currently limited to filling the defect with autologous bone, of which there is not always an abundan...
Front Bioeng Biotechnol — PMID 33195122
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What bone-repair problem do 3D-printed polyurethane scaffolds address? They address critical-size bone defects that cannot heal spontaneously, which commonly result from trauma, infection or surgical tumour resection where autologous bone is limited.
How does the EVOS M5000 evaluate scaffold-supported bone repair? The EVOS M5000 images mesenchymal stem cell and osteoblast behaviour in 3D culture on the scaffolds through brightfield and fluorescence channels, supporting assessment of cell infiltration and matrix deposition.
ZNF521 Represses Osteoblastic Differentiation in Human Adipose-Derived Stem Cells.
Cell type: Adipose-derived stem cells / osteoblasts
Imaging technique: Brightfield/fluorescence imaging
Disease area: Bone regeneration
Human adipose-derived stem cells (hADSCs) are multipotent mesenchymal cells that can differentiate into adipocytes, chondrocytes, and osteocytes. During osteoblastogenesis, the osteoprogenitor cells differentiate into mature osteoblasts and synthesize bone matrix components. Zinc...
Int J Mol Sci — PMID 30567301
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What effect does ZNF521 have on adipose-derived stem cell differentiation? ZNF521 represses osteoblastic differentiation in human adipose-derived stem cells, limiting their progression to mature bone-forming osteoblasts.
How did the EVOS M5000 document osteoblastic differentiation in this study? Brightfield and fluorescence imaging on the EVOS M5000 tracked changes in hADSC morphology and osteogenic marker expression as the cells moved toward mature osteoblasts.
Screening method to identify hydrogel formulations that facilitate myotube formation from encapsulated primary myoblasts.
Cell type: Primary myoblasts
Imaging technique: 3D hydrogel culture, live-cell imaging
Disease area: Muscle regeneration / muscular dystrophy
Hydrogel-based three-dimensional (3D) cellular models are attractive for bioengineering and pharmaceutical development as they can more closely resemble the cellular function of native tissue outside of the body. In general, these models are composed of tissue specific cells embe...
Bioeng Transl Med — PMID 33005743
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What was the aim of screening hydrogel formulations in this muscle study? The screen aimed to identify hydrogel formulations that promote myotube formation from encapsulated primary myoblasts, improving 3D muscle tissue models for bioengineering and drug development.
Why is the EVOS M5000 suited to screening myotube formation in hydrogels? Live-cell brightfield and fluorescence imaging on the EVOS M5000 allows repeated, non-destructive monitoring of myoblast fusion and myotube morphology inside 3D hydrogel cultures across multiple formulations.
Real-time tracking of stem cell viability, proliferation, and differentiation with autonomous bioluminescence imaging.
Cell type: Stem cells (mesenchymal)
Imaging technique: Bioluminescence imaging, live-cell
Disease area: Regenerative medicine / cell therapy
BACKGROUND: Luminescent reporter proteins are vital tools for visualizing cells and cellular activity. Among the current toolbox of bioluminescent systems, only bacterial luciferase has genetically defined luciferase and luciferin synthesis pathways that are functional at the mam...
BMC Biol — PMID 32620121
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What capability did the autonomous bioluminescence reporter bring to stem-cell tracking? It enabled real-time tracking of stem cell viability, proliferation and differentiation without adding external luciferin, because the bacterial luciferase system includes genetically encoded luciferin synthesis.
What complementary role can the EVOS M5000 play alongside bioluminescence tracking? The EVOS M5000 provides brightfield and fluorescence morphology checks that calibrate bioluminescence reporter signals against actual cell number and differentiation state in the same culture wells.
Essential oil impregnated luminescent hydroxyapatite: Antibacterial and cytotoxicity studies.
Cell type: Bacteria / mammalian cells
Imaging technique: Fluorescence microscopy
Disease area: Infection / bone repair
In this study, porous fluorescent nanocrystalline erbium doped hydroxyapatite (eHAp) was synthesized via hydrothermal assisted co-precipitation method. Eucalyptus oil (EU), frankincense oil (FO), Tea tree oil (TTO), wintergreen oil (WO) were successfully absorbed into eHAp pellet...
Mater Sci Eng C Mater Biol Appl — PMID 32806309
Google Scholar | PubMed | DOI
Frequently asked questions about this study
What material did the researchers create for antibacterial and cytotoxicity testing? They synthesized porous fluorescent nanocrystalline erbium-doped hydroxyapatite loaded with essential oils and then tested its antibacterial activity and mammalian cell cytotoxicity.
How does the EVOS M5000 support cytotoxicity and antibacterial assessment of this hydroxyapatite? Fluorescence microscopy on the EVOS M5000 visualises cell viability and bacterial survival in the presence of the luminescent hydroxyapatite, turning material-cytotoxicity assays into reproducible imaging readouts.