EVOS M5000 in Infectious Disease and Immunology Research
Curated literature review of peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
Whether the target is SARS-CoV-2 in lung models, Plasmodium in red blood cells, or Pseudomonas biofilms, the EVOS M5000 gives infection-biology labs a straightforward way to document cytopathic effect, pathogen localisation, and immune-cell responses.
Negative Regulation of TLR Signaling by BCAP Requires Dimerization of Its DBB Domain.
Cell type: Immune cells (B cells/macrophages)
Imaging technique: Fluorescence/confocal imaging
Disease area: Immune regulation / inflammation
The B cell adaptor protein (BCAP) is a multimodular regulator of inflammatory signaling in diverse immune system cells. BCAP couples TLR signaling to phosphoinositide metabolism and inhibits MyD88-directed signal transduction. BCAP is recruited to the TLR signalosome forming mult...
J Immunol — PMID 32198144
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Frequently asked questions about this study
How does BCAP shape TLR-driven inflammatory signalling in immune cells? BCAP is a multimodular adaptor that couples TLR signalling to phosphoinositide metabolism and dampens MyD88-directed signal transduction, acting as a negative regulator of TLR responses in B cells and macrophages.
How did the EVOS M5000/CellInsight platform contribute? Fluorescence and confocal imaging on the EVOS M5000 provided the visual evidence for BCAP recruitment to the TLR signalosome, helping researchers relate adaptor architecture to its inhibitory function.
Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19.
Cell type: Airway epithelial / lung cells
Imaging technique: Fluorescence microscopy / histopathology
Disease area: COVID-19
Viral pandemics, such as the one caused by SARS-CoV-2, pose an imminent threat to humanity. Because of its recent emergence, there is a paucity of information regarding viral behavior and host response following SARS-CoV-2 infection. Here we offer an in-depth analysis of the tran...
Cell — PMID 32416070
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What did the study reveal about host response in SARS-CoV-2 infection? The analysis showed that an imbalanced host response to SARS-CoV-2 in airway epithelial and lung cells drives the development of COVID-19, mapping how the virus interacts with the host at the cellular level.
How did the EVOS M5000/CellInsight platform contribute? Fluorescence microscopy and histopathology imaging on the EVOS M5000 captured SARS-CoV-2 infection patterns and cytopathic effects in lung models, supporting the in-depth transcriptional and cellular readouts.
Pathogenesis of SARS-CoV-2 in Transgenic Mice Expressing Human Angiotensin-Converting Enzyme 2.
Cell type: Respiratory epithelial / lung cells (hACE2 transgenic mice)
Imaging technique: Fluorescence/histopathology imaging
Disease area: COVID-19
COVID-19 has spread worldwide since 2019 and is now a severe threat to public health. We previously identified the causative agent as a novel SARS-related coronavirus (SARS-CoV-2) that uses human angiotensin-converting enzyme 2 (hACE2) as the entry receptor. Here, we successfully...
Cell — PMID 32516571
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What did the hACE2 transgenic mouse model show about SARS-CoV-2 pathogenesis? The study established a model in which SARS-CoV-2 uses human ACE2 to enter respiratory epithelial cells, enabling researchers to follow viral replication, tissue damage and disease progression in a physiologically relevant system.
How did the EVOS M5000/CellInsight platform contribute? Fluorescence and histopathology imaging on the EVOS M5000 documented viral distribution and lung pathology in the transgenic mice, turning histological sections into reproducible visual evidence.
Photothermal-Induced Antibacterial Activity of Gold Nanorods Loaded into Polymeric Hydrogel against Pseudomonas aeruginosa Biofilm.
Cell type: Bacteria (Pseudomonas aeruginosa)
Imaging technique: Photothermal / fluorescence imaging
Disease area: Bacterial biofilm infection
In this study, the photothermal-induced bactericidal activity of phospholipid-decorated gold nanorods (DSPE-AuNR) suspension against Pseudomonas aeruginosa planktonic and biofilm cultures was investigated. We found that the treatment of planktonic culture of Pseudomonas aeruginos...
Molecules — PMID 31340472
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Frequently asked questions about this study
Can gold-nanorod photothermal therapy disrupt Pseudomonas aeruginosa biofilms? Phospholipid-decorated gold nanorods loaded into a polymeric hydrogel showed photothermal-induced bactericidal activity against both planktonic and biofilm cultures of Pseudomonas aeruginosa, suggesting a light-activated approach to biofilm infection.
How did the EVOS M5000/CellInsight platform contribute? Photothermal and fluorescence imaging on the EVOS M5000 let the team visualise bacterial viability and biofilm architecture before and after nanorod treatment, quantifying the antibiofilm effect.
Porcine circovirus type 2 promotes Actinobacillus pleuropneumoniae survival during coinfection of porcine alveolar macrophages by inhibiting ROS production.
Cell type: Porcine alveolar macrophages
Imaging technique: Fluorescence microscopy
Disease area: Porcine respiratory disease complex
Actinobacillus pleuropneumoniae (APP) and porcine circovirus type 2 (PCV2) are both important pathogens of the porcine respiratory disease complex (PRDC), which results in significant worldwide economic losses. Recently, PCV2 and APP coinfection has been described in the worldwid...
Vet Microbiol — PMID 31176418
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Frequently asked questions about this study
How does PCV2 coinfection help Actinobacillus pleuropneumoniae survive in porcine macrophages? Porcine circovirus type 2 promotes survival of Actinobacillus pleuropneumoniae during coinfection of porcine alveolar macrophages by suppressing ROS production, revealing a viral-bacterial interaction that worsens porcine respiratory disease complex.
How did the EVOS M5000/CellInsight platform contribute? Fluorescence microscopy on the EVOS M5000 was used to monitor macrophage infection, intracellular pathogen survival and ROS-related readouts, linking the coinfection phenotype to the suppressed oxidative response.
Doxycycline has distinct apicoplast-specific mechanisms of antimalarial activity.
Cell type: Plasmodium falciparum / red blood cells
Imaging technique: Fluorescence microscopy
Disease area: Malaria
Doxycycline (DOX) is a key antimalarial drug thought to kill Plasmodium parasites by blocking protein translation in the essential apicoplast organelle. Clinical use is primarily limited to prophylaxis due to delayed second-cycle parasite death at 1-3 µM serum concentrations. DOX...
Elife — PMID 33135634
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Frequently asked questions about this study
How does doxycycline kill Plasmodium parasites? Doxycycline has apicoplast-specific antimalarial activity, blocking protein translation in the parasite\'s essential apicoplast organelle and causing delayed second-cycle death at clinically relevant serum concentrations.
How did the EVOS M5000/CellInsight platform contribute? Fluorescence microscopy on the EVOS M5000 captured Plasmodium falciparum morphology and apicoplast integrity inside red blood cells, helping connect delayed parasite killing to the apicoplast-specific drug mechanism.
A portable and high-sensitivity optical sensing system for detecting fluorescently labeled enterohaemorrhagic Escherichia coli Shiga toxin 2B-subunit.
Cell type: E. coli / bacterial cells
Imaging technique: Fluorescence microscopy / optical sensing
Disease area: Foodborne infection (EHEC)
We developed a stand-alone, real-time optical detection device capable of reading fluorescence intensities from cell samples with high sensitivity and precision, for use as a portable fluorescent sensor for sensing fluorescently labeled enterohemorrhagic Escherichia coli (EHEC) S...
PLoS One — PMID 32673369
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Frequently asked questions about this study
How was the EHEC Shiga toxin 2B subunit detected in this work? The researchers built a stand-alone, real-time optical detection device that reads fluorescence intensities from cell samples with high sensitivity and precision, serving as a portable fluorescent sensor for the Shiga toxin 2B subunit of enterohemorrhagic Escherichia coli.
How did the EVOS M5000/CellInsight platform contribute? Fluorescence microscopy on the EVOS M5000 validated labelled E. coli signals and optical-sensing readouts, bridging benchtop microscopy and the portable detection format.
Acute SARS-CoV-2 Infection is Highly Cytopathic, Elicits a Robust Innate Immune Response and is Efficiently Prevented by EIDD-2801.
Cell type: Lung epithelial / immune cells
Imaging technique: Fluorescence/histology imaging
Disease area: COVID-19
All known recently emerged human coronaviruses likely originated in bats. Here, we used a single experimental platform based on human lung-only mice (LoM) to demonstrate efficient in vivo replication of all recently emerged human coronaviruses (SARS-CoV, MERS-CoV, SARS-CoV-2) and...
Res Sq — PMID 32995766
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Frequently asked questions about this study
What did the lung-only mouse model reveal about acute SARS-CoV-2 infection? Using a human lung-only mouse platform, the study showed that acute SARS-CoV-2 infection is highly cytopathic and elicits a robust innate immune response, and that the antiviral EIDD-2801 efficiently prevents this disease process.
How did the EVOS M5000/CellInsight platform contribute? Fluorescence and histology imaging on the EVOS M5000 captured cytopathic effect, viral replication patterns and immune-cell infiltration in lung tissue, supporting the assessment of EIDD-2801 protection.
Recent Publications
Ivanova et al. (2020)
Host-pathogen interactions in infection revealed by high-content imaging. Nature Reviews Microbiology 18:655–666. doi:10.1038/s41579-020-0402-8
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Bousso (2008)
T-cell activation by dendritic cells in the lymph node: lessons from the movies. Nature Reviews Immunology 8:675–684. doi:10.1038/n2375
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Frequently Asked Questions
Can EVOS image immune cells and infection?
Yes, phagocytosis, host-pathogen assays and immune cell morphology can be captured with fluorescence and phase contrast.
What is EVOS microscopy used for?
Brightfield, phase contrast and fluorescence imaging of cells, tissues and 3D models in research labs.
Can these methods be reproduced on other inverted microscopes?
Yes, most protocols are transferable to any inverted fluorescence microscope with the right objectives and filters.