EVOS M5000 in Infectious Disease and Immunology Research
Curated literature review of peer-reviewed studies that used the EVOS M5000 Imaging System. Each paper is summarised by cell type, imaging technique, and disease area, with links to Google Scholar and PubMed. Plankton & Zoom does not host paywalled content.
Whether the target is SARS-CoV-2 in lung models, Plasmodium in red blood cells, or Pseudomonas biofilms, the EVOS M5000 gives infection-biology labs a straightforward way to document cytopathic effect, pathogen localisation, and immune-cell responses.
Negative Regulation of TLR Signaling by BCAP Requires Dimerization of Its DBB Domain.
Cell type: Immune cells (B cells/macrophages)
Imaging technique: Fluorescence/confocal imaging
Disease area: Immune regulation / inflammation
The B cell adaptor protein (BCAP) is a multimodular regulator of inflammatory signaling in diverse immune system cells. BCAP couples TLR signaling to phosphoinositide metabolism and inhibits MyD88-directed signal transduction. BCAP is recruited to the TLR signalosome forming mult...
J Immunol — PMID 32198144
Google Scholar | PubMed | DOI
Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19.
Cell type: Airway epithelial / lung cells
Imaging technique: Fluorescence microscopy / histopathology
Disease area: COVID-19
Viral pandemics, such as the one caused by SARS-CoV-2, pose an imminent threat to humanity. Because of its recent emergence, there is a paucity of information regarding viral behavior and host response following SARS-CoV-2 infection. Here we offer an in-depth analysis of the tran...
Cell — PMID 32416070
Google Scholar | PubMed | DOI
Pathogenesis of SARS-CoV-2 in Transgenic Mice Expressing Human Angiotensin-Converting Enzyme 2.
Cell type: Respiratory epithelial / lung cells (hACE2 transgenic mice)
Imaging technique: Fluorescence/histopathology imaging
Disease area: COVID-19
COVID-19 has spread worldwide since 2019 and is now a severe threat to public health. We previously identified the causative agent as a novel SARS-related coronavirus (SARS-CoV-2) that uses human angiotensin-converting enzyme 2 (hACE2) as the entry receptor. Here, we successfully...
Cell — PMID 32516571
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Photothermal-Induced Antibacterial Activity of Gold Nanorods Loaded into Polymeric Hydrogel against Pseudomonas aeruginosa Biofilm.
Cell type: Bacteria (Pseudomonas aeruginosa)
Imaging technique: Photothermal / fluorescence imaging
Disease area: Bacterial biofilm infection
In this study, the photothermal-induced bactericidal activity of phospholipid-decorated gold nanorods (DSPE-AuNR) suspension against Pseudomonas aeruginosa planktonic and biofilm cultures was investigated. We found that the treatment of planktonic culture of Pseudomonas aeruginos...
Molecules — PMID 31340472
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Porcine circovirus type 2 promotes Actinobacillus pleuropneumoniae survival during coinfection of porcine alveolar macrophages by inhibiting ROS production.
Cell type: Porcine alveolar macrophages
Imaging technique: Fluorescence microscopy
Disease area: Porcine respiratory disease complex
Actinobacillus pleuropneumoniae (APP) and porcine circovirus type 2 (PCV2) are both important pathogens of the porcine respiratory disease complex (PRDC), which results in significant worldwide economic losses. Recently, PCV2 and APP coinfection has been described in the worldwid...
Vet Microbiol — PMID 31176418
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Doxycycline has distinct apicoplast-specific mechanisms of antimalarial activity.
Cell type: Plasmodium falciparum / red blood cells
Imaging technique: Fluorescence microscopy
Disease area: Malaria
Doxycycline (DOX) is a key antimalarial drug thought to kill Plasmodium parasites by blocking protein translation in the essential apicoplast organelle. Clinical use is primarily limited to prophylaxis due to delayed second-cycle parasite death at 1-3 µM serum concentrations. DOX...
Elife — PMID 33135634
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A portable and high-sensitivity optical sensing system for detecting fluorescently labeled enterohaemorrhagic Escherichia coli Shiga toxin 2B-subunit.
Cell type: E. coli / bacterial cells
Imaging technique: Fluorescence microscopy / optical sensing
Disease area: Foodborne infection (EHEC)
We developed a stand-alone, real-time optical detection device capable of reading fluorescence intensities from cell samples with high sensitivity and precision, for use as a portable fluorescent sensor for sensing fluorescently labeled enterohemorrhagic Escherichia coli (EHEC) S...
PLoS One — PMID 32673369
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Acute SARS-CoV-2 Infection is Highly Cytopathic, Elicits a Robust Innate Immune Response and is Efficiently Prevented by EIDD-2801.
Cell type: Lung epithelial / immune cells
Imaging technique: Fluorescence/histology imaging
Disease area: COVID-19
All known recently emerged human coronaviruses likely originated in bats. Here, we used a single experimental platform based on human lung-only mice (LoM) to demonstrate efficient in vivo replication of all recently emerged human coronaviruses (SARS-CoV, MERS-CoV, SARS-CoV-2) and...
Res Sq — PMID 32995766
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