🎬 Watch: EVOS M7000 Analysis in Action
See automated confluence measurement and transfection efficiency analysis — 2 minutes
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The Problem: Manual Cell Culture Analysis
Cell culture labs spend hours every week on repetitive manual tasks:
- Confluence estimation: Looking down the eyepiece, guessing when cells are 80% confluent for passaging
- Transfection checks: Manually counting fluorescent cells to calculate transfection efficiency
- Inconsistent results: Different researchers estimate confluence differently (±20% variation between people)
- No documentation: No images saved to prove confluence was checked before passaging
The Solution: EVOS M7000 Automated Analysis
The EVOS M7000 imaging system with integrated Celleste™ software automates these tasks:
1. Automated Confluence Detection
- Scan entire multi-well plate automatically (6, 12, 24, 96 wells)
- Calculate exact confluence percentage for every well
- Export results to Excel with timestamped images
- Set alerts: notify when wells reach target confluence (e.g., 85%)
2. Transfection Efficiency Analysis
- Count total cells (DAPI/Hoechst stain)
- Count fluorescent cells (GFP/RFP expressing)
- Calculate % transfection automatically
- Compare multiple wells side-by-side
- Export statistics with images for publications
Time Savings in Real Labs
| Task | Manual Method | EVOS M7000 | Time Saved |
|---|---|---|---|
| 96-well confluence check | 45 min (eyepiece + notes) | 5 min automated scan | 40 min (89% faster) |
| Transfection efficiency | 30 min (manual count 3 fields) | 2 min automated | 28 min (93% faster) |
| Weekly reporting | 2 hours (compile images + data) | 10 min (auto-export CSV) | 1 hr 50 min (92% faster) |
Key Specifications
- Imaging: 4 fluorescence channels + brightfield + phase contrast
- Objectives: 4x, 10x, 20x, 40x long working distance
- Plate formats: 6, 12, 24, 48, 96, 384-well plates + slides + dishes
- Software: Celleste™ analysis with confluence, counting, transfection modules
- Output: JPEG, TIFF, PNG, CSV, Excel reports
💡 Pro Tip: Best Settings for Confluence Analysis
For most accurate confluence measurements on the EVOS M7000:
- Use 10x objective for 96-well plates (covers ~80% of well area)
- Use phase contrast instead of brightfield for better cell boundary detection
- Set "Flat Field Correction" ON to eliminate well edge artifacts
- Run background subtraction if medium has debris or precipitate
Applications in Cell Culture Labs
- Routine passage timing: Set 80% confluence alerts, never miss optimal split timing
- Drug screening: Compare confluence across dose-response plates automatically
- Viral production: Monitor HEK293T confluence before transfection for max yield
- CRISPR editing: Verify transfection efficiency before antibiotic selection
- Stem cell culture: Document colony growth rates for protocol optimization
🎬 Watch the Full Video
See the EVOS M7000 analyze confluence and transfection in real time:
▶ Watch on YouTubeRead EVOS M5000 Review →