Hanahan & Weinberg (2011)
Hallmarks of cancer: the next generation. Cell 144:646–674. doi:10.1016/j.cell.2011.02.013
View on DOIEVOS S1000 Spatial Imaging System — Real Publication
The EVOS S1000 is a slide-based multiplex tissue imager launched for spatial-biology and pathology-oriented research. As of September 2025, Thermo Fisher reports one peer-reviewed Nature study using the platform.
Amygdala–liver signalling orchestrates glycaemic responses to stress
Amygdala-liver neural circuitry controls how stress raises blood glucose, bypassing the classical adrenal/pancreas pathway. The study combines spatial imaging with transcriptomics for precise cell phenotyping and circuit mapping. Thermo Fisher reports this as the first peer-reviewed publication using the EVOS S1000 Spatial Imaging System, used alongside Xenium spatial transcriptomics.
Why it matters for EVOS S1000: It demonstrates 9-plex spectral fluorescence imaging of tissue sections in a high-impact neuroscience/metabolism context — exactly the workflow the S1000 is built for.
Read at Nature →
Europe PMC →
Thermo Fisher announcement →
EVOS Imaging Platform — Independent Methods Paper
This open-access PLoS One paper describes a high-throughput imaging and quantification pipeline built around the EVOS imaging platform, with analysis in CellProfiler. It is model-agnostic but validates the EVOS workflow for automated, large-scale cell imaging.
A high-throughput imaging and quantification pipeline for the EVOS imaging platform
The authors present a complete pipeline for brightfield and fluorescence imaging on EVOS, followed by automated segmentation and quantification in CellProfiler. It is a practical methods reference for labs that want to scale EVOS imaging without proprietary high-content software.
EVOS fit: Works with EVOS M5000, M7000 and related systems that export TIFF stacks for open-source analysis.
Read at PLoS One →
PMC full text →
EVOS M7000 Automated Imaging System — Curated Product Citations
Thermo Fisher lists 32 product citations for the EVOS M7000 (catalog AMF7000). We selected 12 that cover the most common UK lab workflows: cancer biology, immunology/infection, neuroscience and stem-cell/regenerative medicine. Each entry links to the PubMed record; click through to the original paper to confirm the exact EVOS configuration used.
Cancer biology and oncology
Clinical utility of circulating tumor-associated cells to predict and monitor chemo-response in solid tumors
Investigates whether circulating tumor-associated cells can predict chemotherapy response in solid tumors. Live-cell and fluorescence imaging of cultured cells is part of the analytical workflow; Thermo Fisher lists this as an EVOS M7000 citation.
PubMed →MicroRNA-103a curtails the stemness of non-small cell lung cancer cells by binding OTUB1 via the Hippo signaling pathway
Functional validation of miR-103a in NSCLC includes imaging-based readouts of stemness and proliferation. Listed by Thermo Fisher as an EVOS M7000 product citation.
PubMed →CD4 T-cell immune stimulation of HER2-positive breast cancer cells alters response to trastuzumab in vitro
Time-resolved microscopy is used to measure how T-cell influence changes HER2-positive breast cancer cell response to trastuzumab. The imaging component is listed as an EVOS M7000 workflow.
PubMed →Norepinephrine-induced DNA damage in ovarian cancer cells
Studies how stress hormones affect DNA integrity in epithelial ovarian cancer, with fluorescence imaging for DNA-damage markers. EVOS M7000 is cited by Thermo Fisher for the imaging work.
PubMed →Infectious disease and immunology
Escape from neutralizing antibodies by SARS-CoV-2 spike protein variants
Pseudovirus neutralization and spike-protein variant characterization relied on cell-culture imaging readouts. The paper is listed by Thermo Fisher as using the EVOS M7000 platform.
PubMed →Co-regulation of the transcription controlling ATF2 phosphoswitch by JNK and p38
Mechanistic study of MAPK signalling and transcription-factor phosphorylation, with fluorescence imaging for cellular reporter assays. Listed as an EVOS M7000 citation.
PubMed →Adenovirus VA RNAI blocks ASC oligomerization and inhibits NLRP3 inflammasome activation
Adenovirus-infected THP-1 macrophage imaging for inflammasome activation and cytokine maturation. Cited by Thermo Fisher for EVOS M7000 imaging.
PubMed →The deubiquitinase STAMBP modulates cytokine secretion through the NLRP3 inflammasome
Investigates STAMBP regulation of IL-1β secretion, with cell-imaging support for inflammasome assays. Listed as an EVOS M7000 product citation.
PubMed →Neuroscience, stem cells and regenerative medicine
Ascorbic acid promotes functional restoration after spinal cord injury partly by epigenetic modulation
Explores axonal regeneration after spinal cord injury, with immunofluorescence imaging of regeneration-associated genes. EVOS M7000 is the cited imaging platform.
PubMed →Guided differentiation of mature kidney podocytes from human induced pluripotent stem cells under chemically defined conditions
A detailed differentiation protocol for podocytes from hiPSCs, with imaging steps for morphology and marker confirmation. Listed by Thermo Fisher as an EVOS M7000 citation.
PubMed →Generation of two human control iPSC cell lines (UCLi016-A and UCLi017-A) from healthy donors with no known ocular conditions
Characterisation of new iPSC lines includes imaging quality-control steps for pluripotency and morphology. Cited by Thermo Fisher for EVOS M7000.
PubMed →Repetitive elements as a transcriptomic marker of aging: evidence in multiple datasets and models
While primarily transcriptomic, the study uses cell-culture imaging in aging models and is listed by Thermo Fisher as an EVOS M7000 citation.
PubMed →
Want the full list? Thermo Fisher maintains the complete 32-citation index for the EVOS M7000 at the product-citations page. We have linked the curated subset above; check the manufacturer's page for the remaining entries.
Thermo Fisher EVOS M7000 citations →
How to Use These Citations
- Grant applications: Cite the methods paper (PLoS One 2020, PMC7406032) and relevant disease-area papers to show EVOS adoption in your field.
- Equipment justification: The S1000 Nature paper and the M7000 oncology/infection citations demonstrate peer-reviewed use in high-impact journals.
- Workflow validation: Read the methods sections via PubMed/PMC to confirm fluorescent markers, plate formats and imaging settings match your assay.
- Procurement: Take the citation list to a Thermo Fisher UK application scientist or authorised distributor to match the exact configuration (objectives, light cubes, stage incubator) used in each study.
Related Plankton & Zoom Guides
- EVOS S1000 Review — slide-based multiplex tissue imager
- EVOS M7000 Review — automated live-cell imaging system
- Spatial Biology in Cancer: EVOS S1000 & M7000 Workflow Guide — maps independent spatial-biology papers to EVOS workflows
- EVOS M7000 & CellInsight CX7 Research Index — curated themed literature reviews
- EVOS M5000 Organoid & Tumoroid Imaging Workflow — 3D cancer model imaging
- Automated Cell Counting Microscope UK — cell counting for tissue dissociation and 3D models
EVOS S1000 review → EVOS M7000 review →
Recent Publications
Sachs et al. (2019)
A living biobank of breast cancer organoids captures disease heterogeneity. Cell 172:373–386. doi:10.1016/j.cell.2017.11.010
View on DOIDrost & Clevers (2018)
Organoids in cancer research. Nature Reviews Cancer 18:407–418. doi:10.1038/s41568-018-0007-6
View on DOILancaster & Knoblich (2014)
Generation of cerebral organoids from human pluripotent stem cells. Nature Protocols 9:2329–2340. doi:10.1038/nprot.2014.158
View on DOIFrequently Asked Questions
What systems do the real publications feature?
EVOS S1000 spatial imaging and EVOS M7000 automated inverted imaging.
Are these papers independently published?
Yes, all entries link to original journal articles or preprints via DOI.