Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
A largely understudied microbially mediated mercury (Hg) bioremediative pathway includes the volatilization of Hg
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Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Cellular senescence is a phenotypic state that contributes to age-related diseases through the secretion of matrix-degrading and inflammatory molecules. An emerging therapeutic strategy for osteoarthritis (OA) is to selectively eliminate senescent cells by initiating apoptosis. This study establishes a cartilage explant model of senescence induction and senolytic clearance usin
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Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Biofilm-mediated infection is a major cause of bone prosthesis failure. The lack of molecules able to act in biofilms has driven research aimed at identifying new anti-biofilm agents via chemical screens. However, to be able to accommodate a large number of compounds, the testing conditions of these screenings end up being typically far from the clinical scenario. In this study
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Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Co-stimulatory signals, cytokines and transcription factors regulate the balance between effector and memory cell differentiation during T cell activation. Here, we analyse the role of the TRAF2-/NCK-interacting kinase (TNIK), a signaling molecule downstream of the tumor necrosis factor superfamily receptors such as CD27, in the regulation of CD8
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the pathogen responsible of coronavirus disease 2019 (COVID-19), has devastated public health services and economies worldwide. Despite global efforts to contain the COVID-19 pandemic, SARS-CoV-2 is now found in over 200 countries and has caused an upward death toll of over 1 million human lives as o
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Reverse genetic systems are a critical tool for studying viruses and identifying countermeasures. In response to the ongoing COVID-19 pandemic, we recently developed an infectious complementary DNA (cDNA) clone for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The reverse genetic system can be used to rapidly engineer viruses with desired mutations to study the
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Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The lack of coronavirus-specific antiviral drugs has instigated multiple drug repurposing studies to redirect previously approved medicines for the treatment of SARS-CoV-2, the coronavirus behind the ongoing COVID-19 pandemic. A recent, large-scale, retrospective clinical study showed that famotidine, when administered at a high dose to hospitalized COVID-19 patients, reduced t
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Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
RNA viruses that replicate in the cytoplasm often disrupt nucleocytoplasmic transport to preferentially translate their own transcripts and prevent host antiviral responses. The
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Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Rapid diagnosis and case isolation are pivotal to controlling the current pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, a label-free DNA capacitive biosensor for the detection of SARS-CoV-2 that demonstrates real-time, low-cost, and high-throughput screening of nucleic acid samples is presented. Our novel biosensor composed of the inte
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Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Type I interferons (IFNs) are our first line of defense against virus infection. Recent studies have suggested the ability of SARS-CoV-2 proteins to inhibit IFN responses. Emerging data also suggest that timing and extent of IFN production is associated with manifestation of COVID-19 severity. In spite of progress in understanding how SARS-CoV-2 activates antiviral responses, m
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Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Crosstalk between immune cells and intestinal stem cells (ISCs)
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Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The influenza A virus (IAV) PA-X protein is a virulence factor that selectively degrades host mRNAs leading to protein shutoff. This function modulates host inflammation, antiviral responses, cell apoptosis, and pathogenesis. In this work we describe a novel approach based on the use of bacteria and plasmid encoding of the PA-X gene under the control of the bacteriophage T7 pro
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Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Shanxi-aged vinegar, a traditional Chinese grain-fermented food that is rich in polyphenols, has been shown to have therapeutic effects on a variety of diseases. However, there has been no comprehensive evaluation of the anti-inflammatory activity of polyphenols extracted from Shanxi-aged vinegar (SAVEP) to date. The anti-inflammatory activities of SAVEP, both in RAW 264.7 macr
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Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
SARS-CoV-2 can infect multiple organs, including lung, intestine, kidney, heart, liver, and brain. The molecular details of how the virus navigates through diverse cellular environments and establishes replication are poorly defined. Here, we generated a panel of phenotypically diverse, SARS-CoV-2-infectible human cell lines representing different body organs and performed long
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Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Heat shock (HS) protein 70 (HSP70), a well-known HS-induced protein, acts as an intracellular chaperone to protect cells against stress conditions. Although HS induces HSP70 expression to confer stress resistance to cells, HS causes cell toxicity by increasing reactive oxygen species (ROS) levels. Recently, a standardized extract of Asparagus officinalis stem (EAS), produced fr
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
SARS-CoV-2, the etiological agent of COVID-19, is characterized by a delay in type I interferon (IFN-I)-mediated antiviral defenses alongside robust cytokine production. Here, we investigate the underlying molecular basis for this imbalance and implicate virus-mediated activation of NF-κB in the absence of other canonical IFN-I-related transcription factors. Epigenetic and sing
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Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Platelet-rich plasma (PRP) is a platelet-based application used to treat osteoarthritis (OA) clinically. The co-stimulatory molecule CD226 is expressed in T cells, NK cells, and also platelets. However, exact effects of CD226 on platelets and whether its expression level influences PRP efficacy are largely unknown. Here, CD226
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Cell type: Endothelial | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Rho-associated coiled-coil-containing protein kinase (ROCK) is a serine/threonine kinase with essential roles in cytoskeletal functions. Substantial evidence implicates ROCK as a critical regulator in the inception and progression of diabetic nephropathy through a mechanism involving mesangial fibrosis, podocyte apoptosis, and endothelial inflammation. Despite these experimenta
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
African swine fever virus (ASFV) is prevalent in many countries and is a contagious and lethal virus that infects pigs, posing a threat to the global pig industry and public health. The interaction between the virus and the host is key to unlocking the mystery behind viral pathogenesis. A comprehensive understanding of the viral and host protein interaction may provide clues fo
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Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Senescent cells constantly experience stressful conditions and restrain their protein translation to cope with it. Here, we present a detailed protocol to measure the rate of global protein synthesis using L-azidohomoalanine (L-AHA)-based click chemistry in human senescent fibroblasts. We optimized several aspects of the procedure, including senescence induction, a flow cytomet
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Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Adeno-associated viral (AAV) vectors are widely used for gene therapy, providing treatment for diseases caused by absent or defective genes. Despite the success of gene therapy, AAV manufacturing is still challenging, with production yields being limited. With increased patient demand, improvements in host cell productivity through various engineering strategies will be necessa
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Cell type: Parasite | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Chagas disease is caused by the protozoan parasite
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Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The most studied mechanism of quorum sensing in Gram-negative bacteria is mediated by autoinducer 1 (AI-1), namely, acyl-homoserine lactone (AHL). This system allows communication among different bacterial species and regulates the expression of virulence genes in many pathogens. Although AHL-producing bacteria have been detected in the intestines of humans and other animals, n
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Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The SARS-CoV-2 (SARS-CoV-2) virus has caused a worldwide pandemic because of the virus's ability to transmit efficiently human-to-human. A key determinant of infection is the attachment of the viral spike protein to the host receptor angiotensin-converting enzyme 2 (ACE2). Because of the presumed zoonotic origin of SARS-CoV-2, there is no practical way to assess the susceptibil
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Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Exposure to organic dust (OD) in agriculture is known to cause respiratory symptoms including loss of lung function. OD exposure activates multiple signaling pathways since it contains a variety of microbial products and particulate matter. Previously, we have shown how OD exposure leads to the secretion of HMGB1 and HMGB1-RAGE signaling, and how this can be a possible therapeu
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Cell type: Parasite | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Isopentenyl pyrophosphate (IPP) is an essential metabolic output of the apicoplast organelle in
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Cell type: B cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Measles virus envelope pseudotyped LV (MV-LV) can achieve high B cell transduction rates (up to 50%), but suffers from low titers. To overcome current limitations, we developed an optimized MV-LV production protocol that achieved consistent B cell transduction efficiency up to 75%. We detail this protocol along with analytical assays to assess the results of MV-LV mediated B ce
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Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Mesenchymal stem cells (MSCs) are multipotent cells found in various tissues and are easily cultivated. For use in clinical protocols, MSCs must be expanded to obtain an adequate number of cells, but a senescence state may be instituted after some passages, reducing their replicative potential. In this study, we report a case where MSC derived from an elderly donor acquired a s
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Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
<i>Haemaphysalis longicornis</i> (Neumann, 1901) (Acari: Ixodidae), the Asian longhorned tick, is an invasive tick species present in the USA since at least 2017 and has been detected in one-third of Virginia counties. While this species is associated with the transmission of multiple pathogens in its native geographical range of eastern Asia, little is known about its ability
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Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Ischemic stroke (IS) is a severe neurological disease that is difficult to recovery. Previous studies have shown that repetitive transcranial magnetic stimulation (rTMS) is a promising therapeutic approach, while the exact therapy mechanisms of rTMS in improving neural functional recovery remain unclear. Furthermore, the inflammatory environment may influence the rehabilitation
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Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Drug repurposing has the advantage of shortening regulatory preclinical development steps. Here, we screened a library of drug compounds, already registered in one or several geographical areas, to identify those exhibiting antiviral activity against SARS-CoV-2 with relevant potency. Of the 1,942 compounds tested, 21 exhibited a substantial antiviral activity in Vero-81 cells.
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
C-type lectin-like receptor 2 (CLEC-2) is highly expressed on platelets and a subpopulation of myeloid cells, and is critical in lymphatic development. CLEC-2 has been shown to support thrombus formation at sites of inflammation, but to have a minor/negligible role in hemostasis. This identifies CLEC-2 as a promising therapeutic target in thromboinflammatory disorders, without
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Reporter-expressing recombinant severe acute respiratory syndrome coronavirus 2 (rSARS-CoV-2) represents an excellent tool to understand the biology of and ease studying viral infections in vitro and in vivo. The broad range of applications of reporter-expressing recombinant viruses is due to the facilitated expression of fluorescence or bioluminescence readouts. In this chapte
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Secondary experimental procedures such as immunostaining have been utilized to study wild-type influenza A viruses (IAV) but are inadequate to rapidly determine the virus in infected cells or for the high-throughput screening (HTS) of antivirals or neutralizing antibodies. Reverse genetics approaches have allowed the generation of recombinant IAV expressing bioluminescent (BL)
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Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Adequate serotonin levels are pivotal to human well-being; thus, serotonin can be used as a biomarker because it regulates a wide range of physical and psychological functions. As an imbalance of serotonin is highly likely to initiate the pathogenesis of various disorders, monitoring serotonin levels in real time is in high demand for the early detection of disease. We fabricat
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Cell type: Amoeba | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Primary amoebic meningoencephalitis (PAM) is a lethal and rapid infection that affects the central nervous system and is caused by the free-living amoeba Naegleria fowleri. The life cycle of this protozoa consists of three different stages: The trophozoite, flagellate and cyst stages. Currently, no fully effective molecules have been found to treat PAM. In the search of new ant
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Despite multifactorial evidence, the safe and effective elimination of free-floating micro-organisms remains a significant scientific challenge. ZeBox Technology exploits microbial Zeta Potential, to extract and eliminate them from free-flowing air, using a non-ionizing electric field, in combination with a microbicidal surface. Evaluation of ZeBox Technology against aerosolize
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Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
African swine fever virus (ASFV) represents a serious threat to the global swine industry, and there are no safe or commercially available vaccines. Previous studies have demonstrated that inactivated vaccines do not provide sufficient protection against ASFV and that attenuated vaccines are effective, but raise safety concerns. Here, we first constructed a deletion mutant in w
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the highly contagious agent responsible for the coronavirus disease 2019 (COVID-19) pandemic. An essential requirement for understanding SARS-CoV-2 biology and the impact of antiviral therapeutics is a robust method to detect the presence of the virus in infected cells or animal models. Despite the development and
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Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Cell size is tightly controlled in healthy tissues, but it is unclear how deviations in cell size affect cell physiology. To address this, we measured how the cell's proteome changes with increasing cell size. Size-dependent protein concentration changes are widespread and predicted by subcellular localization, size-dependent mRNA concentrations, and protein turnover. As prolif
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The canonical function of lentiviral Vif proteins is to counteract the mutagenic potential of APOBEC3 antiviral restriction factors. However, recent studies have discovered that Vif proteins from diverse HIV-1 and simian immunodeficiency virus (SIV) isolates degrade cellular B56 phosphoregulators to remodel the host phosphoproteome and induce G2/M cell cycle arrest. Here, we ev
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Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The small intestinal mucosa constitutes a physical barrier separating the gut lumen from sterile internal tissues. Junctional complexes between cells regulate transport across the barrier, preventing water loss and the entry of noxious molecules or pathogens. Inflammatory diseases in cattle disrupt this barrier; nonetheless, mechanisms of barrier disruption in cattle are poorly
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Cell type: Osteoblast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Osteogenesis imperfecta (OI) is characterized by repeated bone fractures. Recent studies have shown that T lymphocytes and regulatory T cells (Tregs) regulate the functions of osteoclasts and osteoblasts, thus playing a role in bone turnover. We demonstrate an activated effector phenotype and higher secretion of pro-inflammatory cytokines, IFN-γ, and TNF-α in OI peripheral T ce
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
A critical risk factor for head and neck squamous cell carcinoma (HNSCC), particularly of the oropharynx, and the response to radiotherapy is human papillomavirus (HPV) type-16/18 infection. Specifically, HPV-positive HNSCC display increased radiosensitivity and improved outcomes, which has been linked with defective signalling and repair of DNA double-strand breaks (DSBs). Thi
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Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The study aims to elucidate the physicochemical properties and immunomodulatory activity of two polysaccharides (ACP
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Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Type III and type I interferon have similar mechanisms of action, and their different receptors lead to different distributions in tissue. On mucosal surfaces, type III interferon exhibits strong antiviral activity. Porcine epidemic diarrhea virus (PEDV) is an economically important enteropathogenic coronavirus, which can cause a high incidence rate and mortality in
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Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Nitric oxide (NO) is the critical regulator of VEGFR2-induced angiogenesis. Neither VEGF-A over-expression nor L-Arginine (NO-precursor) supplementation has been effective in helping patients with Peripheral Artery Disease (PAD) in clinical trials. One incompletely studied reason may be due to the presence of the less characterized anti-angiogenic VEGF-A (VEGF
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The spread of SARS-CoV-2 has led to a devastating pandemic, with infections resulting in a range of symptoms collectively known as COVID-19. The full repertoire of human tissues and organs susceptible to infection is an area of active investigation, and some studies have implicated the reproductive system. The effects of COVID-19 on human reproduction remain poorly understood,
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Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
<i>Polysiphonia morrowii</i> is a well-known red alga that has promising pharmacological characteristics. The current study evaluates the protective effect of 3-bromo-4,5-dihydroxybenzaldehyde (BDB) isolated from <i>P. morrowii</i> on tumor necrosis factor (TNF)-α/interferon (IFN)-γ-stimulated inflammation and skin barrier deterioration in HaCaT keratinocytes. The anti-inflamma
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Cell type: Cell culture models | Technique: Confocal / fluorescence | Disease/area: Infectious Disease & Immunology
The SARS-CoV-2 pandemic and the urgent need for massive antiviral testing highlighted the lack of a good cell-based assay that allowed for a fast, automated screening of antivirals in high-throughput content with minimal handling requirements in a BSL-3 environment. The present paper describes the construction of a green fluorescent substrate that, upon cleavage by the SARS-CoV
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Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Loss-of-function (LoF) variants in the low-density lipoprotein receptor-related protein 10 gene (
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Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
<i>Helicobacter pylori</i> colonization and persistence could precede gastric adenocarcinoma. Elucidating immune recognition strategies of <i>H. pylori</i> is therefore imperative to curb chronic persistence in the human host. Toll-like receptor 7 (TLR7) and TLR8 are widely known as viral single-stranded RNA (ssRNA) sensors yet less studied in the bacteria context. Here, we inv
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Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Mixed hematopoietic chimerism can promote immune tolerance of donor-matched transplanted tissues, like pancreatic islets. However, adoption of this strategy is limited by the toxicity of standard treatments that enable donor hematopoietic cell engraftment. Here, we address these concerns with a non-myeloablative conditioning regimen that enables hematopoietic chimerism and allo
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Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Killian's (antrochoanal) polyp is a unilateral nasal polypoid lesion of the maxillary sinus especially affecting children and young adults with unilateral nasal obstruction, pus discharge, and headache. Although its etiology is unclear, chronic inflammation, autoreactivity, allergies, and viral infections are implicated in its formation and development, causing nasal tissue rem
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Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Chronic exposure to airborne carbon black ultrafine (nCB) particles generated from incomplete combustion of organic matter drives IL-17A-dependent emphysema. However, whether and how they alter the immune responses to lung cancer remains unknown. Here, we show that exposure to nCB particles increased PD-L1
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