EVOS M5000 in Infectious Disease & Immunology: Independent Studies — Wave 3 Part 5
Peer-reviewed studies discovered independently of Thermo Fisher's curated EVOS M5000 citations. Each paper was full-text verified to mention the EVOS M5000 Imaging System as a microscope. Cards link to Google Scholar, PubMed, PMC and DOI.
EVOS M5000IndependentWave 3
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Insights into growth retardation and dwarfism caused by goose parvovirus in goslings: a transcriptomic profiling study.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Goose parvovirus (GPV) poses a significant threat to the waterfowl industry as it results in a high mortality rate and stunted growth in surviving goslings, leading to significant economic losses. We used 120 goslings and goose embryo fibroblasts inoculated with the GPV SYG61 strain to study the pathogenesis of GPV by pathological and gene expression profile changes. Fourteen d
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Chicken interferon-induced transmembrane proteins inhibit Newcastle disease virus infection by affecting viral entry and W protein expression.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Interferon-induced transmembrane proteins (IFITMs) are essential components of the innate immune system, demonstrating potent resistance to various enveloped viruses (such as influenza, West Nile, and dengue viruses) both in laboratory settings and in living organisms. Newcastle disease (ND), resulting from Newcastle disease virus (NDV), is a severe avian viral ailment with not
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Exogenous prion-like proteins and their potential to trigger cognitive dysfunction.
Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The gut is exposed to a wide range of proteins, including ingested proteins and those produced by the resident microbiota. While ingested prion-like proteins can propagate across species, their implications for disease development remain largely unknown. Here, we apply a multidisciplinary approach to examine the relationship between the biophysical properties of exogenous prion
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WNK1 mediates M-CSF-induced macropinocytosis to enforce macrophage lineage fidelity.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Tissue-resident macrophages (TRM) are critical for mammalian organismal development and homeostasis. Here we report that with-no-lysine 1 (WNK1) controls myeloid progenitor fate, with Csf1r
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GLUT1-mediated HMGB1 O-GlcNAcylation drives hyperglycemia-Induced neutrophil extracellular trap networks formation via TLR4 signaling and exacerbates fibroblast inflammation.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Neutrophil extracellular traps (NETs) exacerbate fibroblast inflammatory injury in hyperglycemic conditions, yet the role of glucose metabolism and O-linked N-acetylglucosamine (O-GlcNAc) glycosylation in this process remains unclear. Here, we investigate how glucose transporter protein 1 (GLUT1)-dependent glucose uptake regulates O-GlcNAcylation of high-mobility group box 1 (H
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HIF-1α Promotes Inflammatory Responses in Aspergillus Fumigatus Keratitis by Activating Pyroptosis Through Caspase-8/GSDMD Pathway.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
This research was designed to explore the expression patterns and functional significance of hypoxia-inducible factor-1α (HIF-1α) in the inflammatory response associated with Aspergillus fumigatus (A. fumigatus) keratitis. Mouse models of A. fumigatus keratitis were created by scraping the corneal epithelium and applying A. fumigatus on the corneal surface. In the in vitro expe
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The macrophage-intrinsic MDA5/IRF5 axis drives HIV-1 intron-containing RNA-induced inflammatory responses.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Despite effective antiretroviral therapy, transcriptionally competent HIV-1 reservoirs remain and contribute to persistent immune activation in people living with HIV (PWH). HIV-1-infected macrophages are important mediators of chronic innate immune activation, though the mechanisms remain unclear. We previously reported that nuclear export and cytoplasmic expression of HIV-1 i
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Human genetic variation reveals FCRL3 is a lymphocyte receptor for Yersinia pestis.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Yersinia pestis is the bacterium responsible for plague, one of the deadliest diseases in history. To discover human genetic determinants of Y. pestis infection, we utilized nearly 1,000 genetically diverse lymphoblastoid cell lines in a cellular genome-wide association study. A nonsynonymous SNP, rs2282284 (N721S), in Fc receptor-like 3 (FCRL3) was associated with bacterial in
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Cas9- and Cas12a-mediated excision and replacement of the celiac disease-related α-gliadin immunogenic complex in hexaploid wheat.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Celiac disease (CD) is a chronic enteropathy affecting approximately 1% of the global population. Wheat α-gliadins are a major contributor to the autoimmune response, as they contain one of the most immunogenic peptides, the 33-mer, along with numerous variants. In this study, we used CRISPR/Cas technology to mutate genes encoding α-gliadins. This approach employed paired sgRNA
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Discovery and development of a safe and efficient COVID-19 mRNA vaccine, STP2104, using a novel capping library screening method.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Messenger RNA (mRNA) vaccines represent a critical avenue for coronavirus disease 2019 (COVID-19) prevention. We developed a COVID-19 mRNA vaccine encoding a codon-optimized full-length ancestral spike (S) protein with a signal peptide, which employs our novel patented co-transcriptional 5'-capping reagent, SmartCap
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B10 Promotes Polarization and Pro-Resolving Functions of Bone Marrow Derived Macrophages (BMDM) Through PD-1 Activation.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Regulatory B cells (B regs) are immune cells that help suppress excessive inflammatory responses by interacting with other immune components. Among them, B-10 cells are known for their strong immunoregulatory function. This study focused on how B-10 cells influence macrophage phenotype and function through the PD-1 signaling pathway. To investigate this, B-10 cells derived from
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Self-powered rapid antigen-specific T-cell response assay for Mycobacterium tuberculosis infections.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Interferon-gamma release assays (IGRAs) that evaluate an individual's T-cell activation response to Mycobacterium tuberculosis (M.tb)-specific peptides serve an important role in diagnosing tuberculosis (TB). However, there are substantial challenges to the use of IGRAs in resource-limited settings. Further, IGRA diagnostic performance can also be compromised in anergic individ
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The swine acute diarrhea syndrome coronavirus spike protein promotes syncytial formation via upregulation of cellular cholesterol synthesis.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel coronavirus that causes acute diarrhea, vomiting, and high mortality in suckling piglets. Research has demonstrated that certain viruses enhance their replication by modulating intracellular cholesterol metabolism. However, the impact of SADS-CoV infection on cellular cholesterol synthesis remains unclear. Here, we
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Mitochondrial transplantation: adaptive bio-enhancement.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Mitochondria, often referred to the powerhouse of the cell, are essential for cellular energy production, and their dysfunction can profoundly affect various organs. Transplantation of healthy mitochondria can restore the bioenergetics in diseased cells and address multiple conditions, but more potentials of this approach remain unclear. In this study, I demonstrated that the s
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In vitro antiviral activities of thymol and Limonin against influenza a viruses and SARS-CoV-2.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Emerging and re-emerging respiratory viruses represent a continuing threat to human health. The pandemic severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and influenza A viruses (IAVs) are co-circulating, presenting serious threats to public health. Therefore, screening for safe and broad-spectrum antiviral candidates to control such viral infections is prioritized.
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Tet Transgene Activation is Disrupted in Lipogenic Triple Negative Breast Cancer Cells.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
A critical challenge for mammalian cell engineering is the unexpected response of transgenes to native transcriptional regulation pathways. One transgene can show different levels of expression at different genomic sites, in different cell types, and under different growth conditions. Collisions between transcription and DNA replication, heterochromatin encroachment, and viral
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Modifying Antifungal Peptides as Safe Food Preservatives.
Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Food loss and waste (FLW) constitute a major global concern with significant environmental and economic ramifications. A major cause of FLW is fungal contamination, leading to food spoilage. Here, we investigate the use of antimicrobial peptides (AMPs) as a new source for food preservatives to combat filamentous fungi and yeast contamination. Specifically, we focused on two mod
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MK2 Inhibition as a Novel Treatment for Fibrosis in Primary Sclerosing Cholangitis via an IL-22-Dependent Mechanism.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Primary sclerosing cholangitis (PSC) is a chronic liver disease characterized by bile duct inflammation and fibrosis, leading to cirrhosis and liver failure. Current therapies are limited to symptom management, with no approved treatments targeting fibrosis. We have identified the MAP kinase-activated protein kinase 2 (MK2) pathway as a potential therapeutic target for treating
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Submicron-sized superantigen biomimetic liposomes with highly efficient pulmonary accumulation to remodel local immune microenvironment for cancer chemoimmunotherapy.
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Metastatic lung cancer continues to cause a high number of deaths due to high malignancy and poor prognosis, and the efficacy of typical chemotherapy or immunotherapy is less than ideal due to the low pulmonary accumulation and targeting of therapeutics. Here, a submicron-sized biomimetic liposome was formulated for the lung-targeted co-delivery of bacterial superantigen and pa
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Protocol for reconstituting enzymatic activities for ultra-large histone methyltransferases NSD1 and SETD2 using a baculovirus expression system.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Expression and reconstitution of large proteins in the human proteome are challenging and often require the use of a mammalian cell expression system that is costly and inefficient. Here, we present a protocol to reconstitute catalytically active, full-length NSD1 (nuclear receptor binding SET domain protein 1) and SETD2 (SET domain containing 2, histone lysine methyltransferas
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A multifunctional T-cell engager containing CD80 enhances prostate cancer treatment.
Cell type: Cancer cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
T-cell engagers (TCEs), the most extensively studied class of bispecific antibodies, redirect effector T cells to tumor cells to induce immune synapse formation, T-cell activation, and subsequent tumor cell killing. However, their therapeutic efficacy in solid tumors is limited by immunosuppressive mechanisms within the tumor microenvironment (TME). To address this challenge, w
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Octenidine effectively reduces Candida auris colonisation on human skin.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Candidozyma (formerly Candida) auris (C. auris), a WHO critical priority pathogen known for its multi-drug resistance and strong skin tropism, is posing a significant health threat. This study evaluates the efficacy of commercial octenidine-based antiseptics in reducing C. auris colonisation on intact and wounded human skin. Using an established ex vivo human skin model to simu
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Commensal colonization of
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The ubiquitous fungal pathogen
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Rapid generation of HCoV-229E and HCoV-OC43 reporter viruses and replicons for antiviral research.
Cell type: Yeast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The large size of coronavirus genome, along with the instability of certain genomic sequences, makes the construction of reverse genetics for coronaviruses particularly challenging. The rapid development and application of reverse genetics systems for coronaviruses require further exploration. Using transformation-associated recombination (TAR) cloning in yeast and the We rapid
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Nrf2/Nlrp3 signaling in aging BMSCs: Traf6 intervention as a novel approach to osteoporosis treatment.
Cell type: Stem cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Senile osteoporosis progression is closely related to the decreased osteogenic differentiation capacity of senescent bone marrow stromal stem cells (BMSCs). This study demonstrated that the Traf6-mediated Nrf2/Nlrp3 signaling axis significantly influences inflammatory senescence progression in BMSCs, and targeting Traf6 can effectively alleviate bone loss caused by inflammatory
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Artesunate Treats Aspergillus fumigatus Keratitis by Inhibiting Fungal Activity and Activating Autophagy Pathway to Reduce Corneal Inflammation.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The purpose of this study was to investigate the therapeutic effect of artesunate (ART) on fungal keratitis (FK). The antifungal properties of ART against Aspergillus fumigatus (A. fumigatus) were confirmed by minimum inhibitory concentration (MIC), biofilm formation inhibition test, propidium iodide fluorescence, and calcium fluorescence white test. The levels of HSP90, BrlA,
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TGF-β1-dependent expression of FOXS1 attenuates adipogenic potential and enhances a myofibroblast cellular phenotype.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
White adipose tissue (WAT) fibrosis is a major determinant of obesity-induced cardiometabolic dysfunction and is characterized by excessive extracellular matrix deposition and myofibroblast activation. Transforming growth factor (TGF)-β1 is a profibrotic cytokine that potently induces myofibroblast activation in adipocyte stem cells (ASCs). How TGF-β1 orchestrates ASC activatio
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Essential function of the integrator complex in Kaposi's sarcoma-associated herpesvirus lytic replication.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The integrator complex (INT) is an essential regulator of RNA biogenesis across evolution. Most current findings describe INT's function in states of equilibrium, presenting a research gap in INT's role in dynamic states, such as in infections and cancers. Viruses hijack cellular RNA machinery to transcribe their genes and produce viral progeny, presenting a unique condition to
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A broadly neutralizing antibody recognizes a unique epitope with a signature motif common across coronaviruses.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Cross-reactive antibodies targeting multiple epitopes have been identified in Sarbecoviruses, but the precise molecular mechanism(s) behind the crossreactivity remain poorly understood. Here, we isolate 3D1, a broadly neutralizing antibody (bnAb) derived from a human combinatorial antibody library targeting the conserved HR1 domain. 3D1 uniquely recognizes a β-turn fold compris
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Cerebral Dopamine Neurotrophic Factor (CDNF) Acts as a Trophic Factor Promoting Neuritogenesis in the Dorsal Root Ganglia (DRG) Neurons Through Activation of the PI3K Signaling Pathway.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The cerebral dopamine neurotrophic factor (CDNF) is a neurotrophic factor extensively studied in the central nervous system because of its neuroprotective effects; however, its role in the peripheral nervous system (PNS) remains less explored. In this study, we used primary dorsal root ganglia (DRG) explants to investigate the neuritogenic potential of exogenous CDNF, as well a
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Complete Loss of Cramp Promotes Experimental Osteoarthritis with Enhanced Chondrocyte Apoptosis in Mice.
Cell type: Chondrocyte | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Osteoarthritis (OA) is the most prevalent form of joint arthritis, frequently associated with aging, mechanical wear, and inflammation. Our previous work demonstrated that cathelicidin-related antimicrobial peptide (Cramp) is upregulated in mouse OA cartilage, and that transient knockdown (KD) of Cramp in cultured chondrocytes decreases IL-1β-induced expression of matrix-degrad
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Repurposing Analysis of Nitroxoline (8-Hydroxy-5-nitroquinoline) as an Antichagasic Compound.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
<b>Background/Objectives:</b> Chagas disease, caused by the protozoan parasite <i>Trypanosoma cruzi</i>, remains a major neglected tropical disease, with over six million cases concentrated, primarily in Latin America. Despite decades of research, treatment continues to rely on two outdated drugs-benznidazole and nifurtimox-both of which exhibit limited efficacy and are associa
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IDHP Mitigates LPS-Induced Cardiomyocyte Injury via the GAS6/Axl-AMPK Axis: A Multi-Target Strategy Counteracting Inflammation, Oxidative Stress, and Apoptosis.
Cell type: Cardiomyocyte | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
<b>Background:</b> Sepsis-induced myocardial injury (SIMI) significantly contributes to sepsis-related mortality, yet effective therapies remain limited. This study investigated the cardioprotective potential of isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate (IDHP), a bioactive metabolite from <i>Salvia miltiorrhiza</i>, focusing on its mechanism via the GAS6/Axl signali
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Type A cholesterol-dependent cytolysins translocate to the trans-Golgi network for NLRP3 inflammasome activation.
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Cholesterol-dependent cytolysins (CDCs) constitute the largest group of pore-forming toxins and serve as critical virulence factors for diverse pathogenic bacteria. Several CDCs are known to activate the NLRP3 inflammasome, although the mechanisms are unclear. Here we discovered that multiple CDCs, which we referred to as type A CDCs, were internalized and translocated to the t
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Shiga Toxin Type 2 Aggravates G1/S Phase Cell Cycle Arrest, Mediating Caspase-Independent Cell Death under Hyperosmotic Conditions in the Kidney.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Shiga toxin (Stx) is a virulence factor produced by
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Aging-associated DNA methylation of LEF1 modulates inflammation and neurodegenerative pathways.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Aging is accompanied by profound changes in immune regulation and epigenetic landscapes, yet the molecular drivers underlying these alterations are not fully understood. Transcriptional profiles of peripheral blood samples from young and elderly individuals, together with aging-associated methylation probe data, were used to identify aging biomarkers. Transcriptomics and chroma
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Breaking the reproducibility barrier with standardized protocols for plant-microbiome research.
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Inter-laboratory replicability is crucial yet challenging in microbiome research. Leveraging microbiomes to promote soil health and plant growth requires understanding underlying molecular mechanisms using reproducible experimental systems. In a global collaborative effort involving five laboratories, we aimed to help advance reproducibility in microbiome studies by testing our
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Shiitake Mushroom-Derived Vesicle-like Nanoparticles Improve Cognitive Function and Reshape Gut Microbiota and Fecal Metabolome in Aged Mice.
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Population aging and its associated chronic conditions have become an unprecedented challenge in the United States and worldwide. Many aged individuals experience certain forms of cognitive decline, which increases their risk of developing a pre-dementia condition called mild cognitive impairment and even dementia. No effective pharmacological treatments are available to treat
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Inhibiting TIRAP-mediated inflammatory signaling: A promising therapeutic strategy against sepsis.
Cell type: Bacteria | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
The timely therapeutic targeting of the dysregulated immune response in sepsis is essential to restore immune homeostasis and prevent progression to organ dysfunction. In this study, we investigated whether a combination therapy with an antibiotic exhibiting anti-inflammatory properties and the anti-inflammatory compound dorzolamide will improve the bacterial sepsis outcome. Us
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Dynamic cholangiocyte responses in a murine model of reversible cholestasis: macrophage remodeling and NF-Y-mediated TGFβ1 expression.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
During cholestasis, cholangiocytes become activated, promoting macrophage-associated periductal infiltration and fibrosis. The cholangiocyte-specific mechanisms responsible for these processes are unclear. To gain insight into the cholangiocyte signaling mechanisms contributing to these pathophysiologic processes, mice were fed a 3,5-diethoxycarbonyl-1,4-dihydro-collidine (DDC)
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Detection of electrical signals in fungal mycelia in response to external stimuli.
Cell type: Cell culture models | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Electrical signaling is a crucial mechanism for intercellular communication across diverse biological systems. Despite evidence of electrical activity in fungal mycelia, a standardized, reproducible method for detecting these signals is lacking. In this study, we developed a novel approach using printed circuit boards with embedded differential electrodes to record extracellula
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Nanoparticle-boosted myeloid-derived suppressor cell therapy for immune reprogramming in multiple sclerosis.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Massive immune cell infiltration and persistent inflammation in the central nervous system (CNS) are key hallmarks of multiple sclerosis. Here, we report a myeloid-derived suppressor cell (MDSC)-based therapeutic strategy, named CNS Immune Targeting Enabled by MDSCs (CITED), which uses surface-decorated MDSCs carrying rapamycin nanoparticles (NPs) for targeted multimodal immune
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Iron overload-induced ferroptosis in CD8
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Endometriosis (EM) exhibits localised iron overload. However, the contribution of ferroptosis to EM pathogenesis remains unclear. We investigated how iron overload affects CD8⁺ T cell immune function and the underlying ferroptotic mechanisms. We collected eutopic and ectopic endometrial tissues from 57 patients with stage III-IV EM and eutopic tissues from 31 controls. Iron dep
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LMT2368 (1-(4-Chlorophenyl)-3-(3-fluoro-5-(trifluoromethyl)phenyl)urea) Negatively Regulates Inflammation by Inhibiting NLRP3 Inflammasome Activation.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
<b>Background/Objectives</b>: The dysregulation of NLRP3 inflammasome activation has been established as a key driver of inflammatory disease pathology, which marks NLRP3 as an attractive therapeutic target. However, the clinical development of NLRP3 inhibitors such as MCC950 has been hampered by their associated toxicity profiles, highlighting an unmet clinical need. <b>Method
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CTRP6 as a negative regulator of anti-inflammatory M2 macrophage polarization.
Cell type: Macrophage | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Chronic low-grade inflammation in adipose tissue, primarily driven by macrophages, plays a central role in obesity pathophysiology. C1q/TNF-related protein 6 (CTRP6), a member of the CTRP family, has emerged as a key regulator of this inflammatory process. Here, we demonstrate that CTRP6 expression is upregulated in adipose tissue macrophages during obesity, where it acts as a
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Biofilm-Antagonist Ginger-Based 3D-Printable Photoresins for Complex Implant Designs Exhibiting Advanced Multifunctional Biomedical Applications.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Since the discovery of 3D-printing, it has revolutionized personalized drug delivery and implants by enabling intricate, customizable designs. However, key challenges remain, including complex design, host immune response, biofilm formation, and infection-induced inflammation at the implant site. This work offers, first-ever, unique ginger-based 3D-printable resins by chemicall
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Parechovirus-3 infection disrupts immunometabolism and leads to glutamate excitotoxicity in neural organoids.
Cell type: Neuron | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Parechovirus ahumpari 3 (HPeV-3) is among the main agents causing severe neonatal neurological infections such as encephalitis and meningitis. However, the underlying molecular mechanisms and changes to the host cellular landscape leading to neurological disease has been understudied. Through quantitative proteomic analysis of HPeV-3 infected neural organoids, we identified uni
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Cytokine responses to respiratory syncytial virus infection in well-differentiated infant nasal epithelial cell cultures.
Cell type: Epithelial | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Differentiated airway epithelial cell cultures closely mimic the in vivo airways. We compared the structure and cytokine response of well-differentiated human nasal epithelial (HNE) cells obtained from healthy infants (iHNE) and adults (aHNE). Human bronchial epithelial (HBE) progenitor (n=4) and primary HNE cells (n=4 aHNE; n=10 iHNE) were cultured using a modification of the
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A DNA vaccine based on hemagglutinin and conserved epitopes of influenza B virus provides cross-lineage protection in mice.
Cell type: T cell | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Conventional influenza vaccine can prevent infection and reduce the risk of post-infection complications. However, they lack the capacity to effectively respond to influenza virus mutations. This results in the vaccine becoming ineffective due to a reduced antigenic match. It is necessary to develop a new strategy for vaccine that will provide broad cross-reactive protection. A
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Tandem split-GFP influenza A viruses for sensitive and accurate replication analyses.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Reporter influenza A viruses (IAVs) are valuable tools for studying virus fitness, screening antivirals, and assessing host-virus interactions. However, the compact and segmented nature of the IAV genome presents major challenges for engineering genetically stable reporter viruses with minimal fitness defects. To address this, we developed a replication-competent IAV incorporat
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Structural insights into mechanisms of zinc scavenging by the Candida albicans zincophore Pra1.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Candida albicans causes over 400,000 life-threatening, and an additional half a billion of mucosal infections annually. In response to infection, the host limits essential micronutrient availability, including zinc, to restrict growth of the invading pathogen. As assimilation of zinc is essential for C. albicans pathogenicity, limitation induces secretion of the zincophore prot
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CRE-Ter enhances murine bone differentiation, improves muscle cell atrophy, and increases irisin expression.
Cell type: Osteoblast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Ternary complex of curcuminoid-rich extract (CRE-Ter) is a developed water-soluble Curcuma longa extract containing 14% w/w curcuminoids, hydroxypropyl-β-cyclodextrin, and polyvinylpyrrolidone K30. This study aimed to investigate the biomolecular effects of CRE-Ter on differentiation of bone cells (murine MC3T3-E1 preosteoblasts), muscle cells (murine dexamethasone-treated C2C1
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Molecular mechanism of resistance to lonafarnib conferred by mutations in the cysteine-rich region of respiratory syncytial virus fusion glycoprotein and discovery of a lonafarnib-derived antiviral PROTAC.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Lonafarnib, an oral antiviral that targets the fusion glycoprotein of respiratory syncytial virus (RSV), has demonstrated efficacy
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A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection.
Cell type: Biological samples | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
Rotavirus (RV) infection remains a leading cause of hospitalization and mortality among infants and young children. Despite global implementation of RV vaccines, hundreds of thousands of infants and young children still succumb to this disease each year due to ineffective treatment. In this study, we demonstrated that NVP-HSP990, a novel small-molecule heat shock protein 90 (HS
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Alkaline Bromodeoxyuridine (BrdU) Comet Assay to Detect Replication-Associated DNA Damage.
Cell type: Fibroblast | Technique: Fluorescence imaging | Disease/area: Infectious Disease & Immunology
DNA replication is often challenged by endogenous and exogenous sources of DNA damage, which can stall replication forks and result in single-stranded DNA (ssDNA) gaps, double-strand breaks (DSBs), and genomic instability. Detecting DNA damage specifically in newly synthesized DNA strands is essential for understanding how eukaryotic cells respond to replication stress and cont
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