Streamlined spatial analysis of the tumor microenvironment and associated disease states using multiplex immunohistochemistry
2025 Journal of Immunology abstract describing a streamlined multiplex immunohistochemistry workflow for spatial analysis of tumor microenvironments and disease states. The work aligns with the EVOS S1000’s role in making multiplex tissue imaging accessible for translational immuno-oncology studies.
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What problem does this workflow solve? The authors present a streamlined multiplex immunohistochemistry approach for spatially dissecting the tumor microenvironment and linked disease states, reducing the practical barriers to mapping multiple tissue markers in a single section.
How does the EVOS S1000 fit into the workflow? With its accessible multiplex fluorescence and slide-scanning setup, the EVOS S1000 is well suited to capture the multiple stained markers and spatial relationships described in the workflow, bringing high-plex tissue analysis within reach of translational immuno-oncology labs.
Cellular phenotyping of tumor microenvironments with a high-plex fluorescent labeling and spectral unmixing approach
2025 Journal of Immunology abstract on high-plex fluorescent labeling and spectral unmixing to phenotype tumor microenvironments. The approach matches the spectral multiplexing and unmixing capabilities built into the EVOS S1000 Spatial Imaging System.
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What is the paper’s main contribution? It demonstrates how high-plex fluorescent labelling combined with spectral unmixing can phenotype diverse cell populations inside tumor microenvironments, going beyond simple marker presence to resolve overlapping signals in complex tissue.
How did the EVOS S1000 enable this phenotyping? The EVOS S1000’s built-in spectral multiplexing and unmixing capabilities let researchers separate closely overlapping fluorophores on tissue sections, which is exactly the capability needed to turn densely labelled tumor microenvironment samples into cleanly resolved cell phenotypes.
a high-throughput imaging and quantification pipeline for the EVOS imaging platform
2020 PLOS One methods article presenting a high-throughput imaging and quantification pipeline built around the EVOS imaging platform. While published before the S1000 launch, the pipeline principles — batch acquisition, standardised export formats and automated quantification — are directly applicable to EVOS S1000 spatial-imaging workflows.
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What does this methods paper deliver? It introduces an automated high-throughput imaging and quantification pipeline built around the EVOS platform, combining batch image acquisition with standardised export formats and downstream automated analysis for scalable cell assays.
Why is this relevant to the EVOS S1000? The pipeline principles of batch acquisition, consistent data export and automated quantification map directly onto EVOS S1000 spatial-imaging workflows, giving labs a ready-made route to scale up multiplex tissue imaging projects on the same platform family.
Moving forward with multiplexing
2024 feature in The Pathologist discussing how the EVOS S1000 Spatial Imaging System improves scanning and processing of stained multiplexed tissue samples. The article highlights faster slide scanning, cleaner unmixing and a biology-focused interface for pathology and spatial-biology labs.
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What does this feature article cover? It discusses practical progress in multiplexed tissue imaging, highlighting faster slide scanning, cleaner spectral unmixing and interfaces that let pathologists focus on biology rather than instrument complexity.
How does the EVOS S1000 advance the themes in the article? The EVOS S1000 Spatial Imaging System exemplifies the shift toward simpler multiplexing by combining rapid whole-slide scanning, spectral unmixing and a biologist-friendly interface in one pathology-oriented spatial platform.
Evaluation of antibody labelling kits for multiplex imaging of tissue sections
2025 KTH Royal Institute of Technology thesis evaluating antibody-labelling kits for multiplex imaging of tissue sections. The work supports the reagent-validation step that underpins reliable multiplex immunofluorescence on spatial imaging systems such as the EVOS S1000.
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What is the practical outcome of this thesis? It evaluates and compares antibody-labelling kits for multiplex imaging of tissue sections, helping researchers choose reagent chemistries that produce reliable, reproducible staining before moving to high-plex spatial acquisition.
How does the EVOS S1000 connect to this validation step? Because the EVOS S1000 is designed for multiplex immunofluorescence on tissue sections, the reagent-validation criteria tested in the thesis directly determine the quality of the spectral images and downstream protein maps it can produce.
Combining protein interactions with cellular phenotyping to predict response to immunotherapy
2024 KTH thesis exploring how protein-interaction data and cellular phenotyping can together predict immunotherapy response in metastatic bladder cancer. The phenotyping layer is conceptually aligned with high-plex spatial imaging workflows such as those enabled by the EVOS S1000.
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Frequently asked questions about this study
What approach does this thesis explore? It combines protein-interaction data with cellular phenotyping to predict immunotherapy response in metastatic bladder cancer, layering molecular wiring information onto cell-level observations for a richer predictive model.
How did the EVOS S1000 relate to the cellular phenotyping? The high-plex spatial imaging workflow enabled by the EVOS S1000 is conceptually aligned with the phenotyping layer, offering a way to capture many cell states and their spatial context in bladder cancer tissue in support of response prediction.
Accelerate and simplify spatial proteomics for translational research with the EVOS S1000 Spatial Imaging System
2025 on-demand webinar from LabRoots featuring Thermo Fisher Scientific, focused on how the EVOS S1000 simplifies spatial proteomics entry for translational research. It covers 9-plex spectral fluorescence imaging, tissue-slide workflows and rapid data generation compared to traditional spatial platforms.
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What is the webinar’s central message? It shows how the EVOS S1000 can lower the barrier to entry for spatial proteomics in translational research by delivering 9-plex spectral fluorescence imaging, tissue-slide workflows and faster data generation than traditional spatial platforms.
How does the EVOS S1000 itself enable this simplification? By integrating 9-colour spectral imaging, tissue-slide handling and streamlined analysis in one system, the EVOS S1000 removes the need for a dedicated core-facility instrument while still producing multiplex protein maps from intact tissue sections.
Spectral multiplex fluorescence imaging with the Invitrogen EVOS S1000 system
2025 technology spotlight demonstrating spectral multiplex fluorescence imaging on the EVOS S1000. The presentation focuses on practical panel design, spectral unmixing and generating high-quality spatial protein maps without the complexity of a core-facility instrument.
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Frequently asked questions about this study
What does this technology spotlight demonstrate? It walks through spectral multiplex fluorescence imaging on the EVOS S1000, focusing on practical panel design, spectral unmixing and the generation of high-quality spatial protein maps without the complexity of a core-facility instrument.
What makes the EVOS S1000 central to the spotlight? The system’s spectral multiplexing and unmixing engine is the key technology being showcased; it is what lets users design compact, multi-marker panels and extract clean, spatially resolved protein maps from tissue slides.