Fluorescent marker prediction for non-invasive optical imaging in bovine satellite cells using deep learning
A 2025 Frontiers in Artificial Intelligence brief report that predicts DAPI and other fluorescent markers directly from brightfield EVOS M5000 images of bovine satellite cells using a U-Net convolutional neural network. It demonstrates how the EVOS M5000 can be used as a non-invasive imaging platform to train AI models that reduce the need for destructive fluorescent staining in cultured-cell quality control.
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Improving viral-based gene delivery to mammalian cells through simple co-incubation with transportan peptide in vitro
A 2025 Journal of Pharmaceutical Sciences study that used the EVOS M5000 to image mammalian cells and evaluate improvements in viral gene-delivery efficiency after co-incubation with transportan peptide. The work shows how routine fluorescence imaging on the EVOS M5000 supports quantitative readouts in gene-therapy process development.
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Single-cycle parainfluenza virus type 5 vectors for producing recombinant proteins, including a humanized anti-V5 tag antibody
A 2025 open-access research article from St George’s, University of London, describing single-cycle PIV5 vectors engineered for recombinant protein production. Fluorescence imaging performed on the EVOS M5000 was used to confirm reporter expression and vector transduction in cell culture, illustrating the system’s utility in viral-vector characterisation.
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Harnessing cell size to separate genetically and functionally distinct dental pulp-derived mesenchymal stromal cell subpopulations
A 2025 Journal of Biological Engineering paper that separated dental-pulp mesenchymal stromal-cell subpopulations by size and then used the EVOS M5000 for downstream fluorescence imaging to confirm cell identity and functional differences. The study highlights how the EVOS M5000 supports cell-therapy and regenerative-medicine characterisation workflows.
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Inhibition of early EHDV2-Ibaraki infection steps in bovine cells by endosome alkalinization or ikarugamycin, but not by blockage of individual endocytic pathways
A 2025 virology study that employed the EVOS M5000 to image bovine cells during early Epizootic Haemorrhagic Disease Virus infection. The microscope helped visualise viral entry and the impact of endosome-modulating compounds, demonstrating its value in infection-biology time-course imaging.
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Nanoplastic toxicity and uptake in kidney cells: differential effects of concentration, particle size, and polymer type
A 2025 Research Square preprint that used the EVOS M5000 to image nanoplastic uptake and toxicity in kidney cell cultures across different particle sizes and polymer chemistries. The work shows the system’s application in environmental toxicology and high-content phenotyping.
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APPswe mutation causes functional deficits in endothelial cells generated by transient ETV2 overexpression in human iPSCs
A 2025 Research Square preprint investigating an Alzheimer’s-related APPswe mutation in iPSC-derived endothelial cells. The EVOS M5000 was used for live-cell and fluorescence imaging to assess endothelial morphology and functional markers, linking the microscope to neurovascular disease modelling.
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Detection of Organelle-Specific Dyes Labeled Extracellular Vesicles with Colocalization-Fluorescence Nanoparticle Tracking Analysis
A 2026 ACS Omega article on extracellular-vesicle characterisation. The EVOS M5000 was used alongside nanoparticle-tracking analysis to image and validate organelle-specific dye labelling of EVs, supporting multi-modal analytical workflows in nanomedicine.
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